In Vivo Adipogenesis in Rats Measured by Cell Kinetics in Adipocytes and Plastic-Adherent Stroma-Vascular Cells in Response to High-Fat Diet and Thiazolidinedione
Impairment of adipogenesis contributes to the development of obesity-related insulin resistance. The current in vitro approaches for its assessment represent crude estimates of the adipogenic potential because of the disruption of the in vivo microenvironment. A novel assessment of in vivo adipogene...
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| Veröffentlicht in: | Diabetes (New York, N.Y.) N.Y.), 2012-01, Vol.61 (1), p.137-144 |
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| creator | TCHOUKALOVA, Yourka D FITCH, Mark ROGERS, Pamela M COVINGTON, Jeffrey D HENAGAN, Tara M JIANPING YE HELLERSTEIN, Marc K RAVUSSIN, Eric |
| description | Impairment of adipogenesis contributes to the development of obesity-related insulin resistance. The current in vitro approaches for its assessment represent crude estimates of the adipogenic potential because of the disruption of the in vivo microenvironment. A novel assessment of in vivo adipogenesis using the incorporation of the stable isotope deuterium ((2)H) into the DNA of isolated adipocytes and stroma-vascular fraction from adipose tissue has been developed. In the current study, we have refined this technique by purifying the adipocytes via a negative immune selection and sorting the plastic adherent stroma-vascular (aSV) subfraction (using 3 h culture) that contains mostly adipocyte progenitor cells and ∼10% of small adipocytes. Using a 3-week 8% (2)H(2)O ingestion with a high-fat diet (HFD) or HFD plus pioglitazone (HFD-P), we demonstrate that the fractions of new aSV cells (f(aSV)) and immunopurified adipocytes (f(AD)) (the ratio of their (2)H-enrichment of DNA to the maximal (2)H-enrichment of DNA of bone marrow reference cells) recapitulate the known hyperplastic mechanism of weight gain with pioglitazone treatment. We conclude that f(aSV) and f(AD) are reliable indices of in vivo adipogenesis. The proposed method represents a valuable tool for studying the effect of interventions (drugs, diets, and exercise) on in vivo adipogenesis. |
| doi_str_mv | 10.2337/db10-1768 |
| format | Article |
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The current in vitro approaches for its assessment represent crude estimates of the adipogenic potential because of the disruption of the in vivo microenvironment. A novel assessment of in vivo adipogenesis using the incorporation of the stable isotope deuterium ((2)H) into the DNA of isolated adipocytes and stroma-vascular fraction from adipose tissue has been developed. In the current study, we have refined this technique by purifying the adipocytes via a negative immune selection and sorting the plastic adherent stroma-vascular (aSV) subfraction (using 3 h culture) that contains mostly adipocyte progenitor cells and ∼10% of small adipocytes. Using a 3-week 8% (2)H(2)O ingestion with a high-fat diet (HFD) or HFD plus pioglitazone (HFD-P), we demonstrate that the fractions of new aSV cells (f(aSV)) and immunopurified adipocytes (f(AD)) (the ratio of their (2)H-enrichment of DNA to the maximal (2)H-enrichment of DNA of bone marrow reference cells) recapitulate the known hyperplastic mechanism of weight gain with pioglitazone treatment. We conclude that f(aSV) and f(AD) are reliable indices of in vivo adipogenesis. The proposed method represents a valuable tool for studying the effect of interventions (drugs, diets, and exercise) on in vivo adipogenesis.</description><identifier>ISSN: 0012-1797</identifier><identifier>EISSN: 1939-327X</identifier><identifier>DOI: 10.2337/db10-1768</identifier><identifier>PMID: 22124466</identifier><identifier>CODEN: DIAEAZ</identifier><language>eng</language><publisher>Alexandria, VA: American Diabetes Association</publisher><subject>Adipocytes ; Adipocytes - drug effects ; Adipocytes - physiology ; Adipogenesis - drug effects ; Adipogenesis - physiology ; Adipose tissue ; Adipose tissues ; Animals ; Biological and medical sciences ; Body fat ; Bone marrow ; Calories ; Cell Adhesion ; Cell Culture Techniques - instrumentation ; Cell Differentiation - drug effects ; Cell Differentiation - physiology ; Cells, Cultured ; Development and progression ; Diabetes. Impaired glucose tolerance ; Diet ; Diet, High-Fat ; Dietary Fats - pharmacology ; Endocrine pancreas. Apud cells (diseases) ; Endocrinopathies ; Endothelial Cells - cytology ; Endothelial Cells - drug effects ; Endothelial Cells - physiology ; Etiopathogenesis. Screening. Investigations. Target tissue resistance ; Fat cells ; Intervention ; Kinetics ; Laboratories ; Male ; Medical sciences ; Obesity ; Obesity Studies ; Physiological aspects ; Plastics ; Rats ; Rats, Long-Evans ; Stem cells ; Stromal Cells - cytology ; Stromal Cells - drug effects ; Stromal Cells - physiology ; Thiazolidinediones - pharmacology</subject><ispartof>Diabetes (New York, N.Y.), 2012-01, Vol.61 (1), p.137-144</ispartof><rights>2015 INIST-CNRS</rights><rights>COPYRIGHT 2012 American Diabetes Association</rights><rights>COPYRIGHT 2012 American Diabetes Association</rights><rights>Copyright American Diabetes Association Jan 2012</rights><rights>2012 by the American Diabetes Association. 2012</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c608t-cbc3283e9691d8a93b6b1efd2d969999002bb64ea24fab6152cb1e73668dc8793</citedby><cites>FETCH-LOGICAL-c608t-cbc3283e9691d8a93b6b1efd2d969999002bb64ea24fab6152cb1e73668dc8793</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3237665/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3237665/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=25488177$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22124466$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>TCHOUKALOVA, Yourka D</creatorcontrib><creatorcontrib>FITCH, Mark</creatorcontrib><creatorcontrib>ROGERS, Pamela M</creatorcontrib><creatorcontrib>COVINGTON, Jeffrey D</creatorcontrib><creatorcontrib>HENAGAN, Tara M</creatorcontrib><creatorcontrib>JIANPING YE</creatorcontrib><creatorcontrib>HELLERSTEIN, Marc K</creatorcontrib><creatorcontrib>RAVUSSIN, Eric</creatorcontrib><title>In Vivo Adipogenesis in Rats Measured by Cell Kinetics in Adipocytes and Plastic-Adherent Stroma-Vascular Cells in Response to High-Fat Diet and Thiazolidinedione</title><title>Diabetes (New York, N.Y.)</title><addtitle>Diabetes</addtitle><description>Impairment of adipogenesis contributes to the development of obesity-related insulin resistance. The current in vitro approaches for its assessment represent crude estimates of the adipogenic potential because of the disruption of the in vivo microenvironment. A novel assessment of in vivo adipogenesis using the incorporation of the stable isotope deuterium ((2)H) into the DNA of isolated adipocytes and stroma-vascular fraction from adipose tissue has been developed. In the current study, we have refined this technique by purifying the adipocytes via a negative immune selection and sorting the plastic adherent stroma-vascular (aSV) subfraction (using 3 h culture) that contains mostly adipocyte progenitor cells and ∼10% of small adipocytes. Using a 3-week 8% (2)H(2)O ingestion with a high-fat diet (HFD) or HFD plus pioglitazone (HFD-P), we demonstrate that the fractions of new aSV cells (f(aSV)) and immunopurified adipocytes (f(AD)) (the ratio of their (2)H-enrichment of DNA to the maximal (2)H-enrichment of DNA of bone marrow reference cells) recapitulate the known hyperplastic mechanism of weight gain with pioglitazone treatment. We conclude that f(aSV) and f(AD) are reliable indices of in vivo adipogenesis. The proposed method represents a valuable tool for studying the effect of interventions (drugs, diets, and exercise) on in vivo adipogenesis.</description><subject>Adipocytes</subject><subject>Adipocytes - drug effects</subject><subject>Adipocytes - physiology</subject><subject>Adipogenesis - drug effects</subject><subject>Adipogenesis - physiology</subject><subject>Adipose tissue</subject><subject>Adipose tissues</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Body fat</subject><subject>Bone marrow</subject><subject>Calories</subject><subject>Cell Adhesion</subject><subject>Cell Culture Techniques - instrumentation</subject><subject>Cell Differentiation - drug effects</subject><subject>Cell Differentiation - physiology</subject><subject>Cells, Cultured</subject><subject>Development and progression</subject><subject>Diabetes. Impaired glucose tolerance</subject><subject>Diet</subject><subject>Diet, High-Fat</subject><subject>Dietary Fats - pharmacology</subject><subject>Endocrine pancreas. Apud cells (diseases)</subject><subject>Endocrinopathies</subject><subject>Endothelial Cells - cytology</subject><subject>Endothelial Cells - drug effects</subject><subject>Endothelial Cells - physiology</subject><subject>Etiopathogenesis. Screening. Investigations. Target tissue resistance</subject><subject>Fat cells</subject><subject>Intervention</subject><subject>Kinetics</subject><subject>Laboratories</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Obesity</subject><subject>Obesity Studies</subject><subject>Physiological aspects</subject><subject>Plastics</subject><subject>Rats</subject><subject>Rats, Long-Evans</subject><subject>Stem cells</subject><subject>Stromal Cells - cytology</subject><subject>Stromal Cells - drug effects</subject><subject>Stromal Cells - physiology</subject><subject>Thiazolidinediones - pharmacology</subject><issn>0012-1797</issn><issn>1939-327X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>BEC</sourceid><sourceid>BENPR</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNp9kltv0zAUxyMEYmXwwBdAFgghHjJ8SZ3kZVJV2EUUDcGYeLMc-6T1lNolTibKx-GTctKWwVCF_WDpnN_5-9yS5CmjR1yI_I2tGE1ZLot7yYiVokwFz7_eT0aUMo72Mj9IHsV4TSmVeB8mB5wznmVSjpKf555cuZtAJtatwhw8RBeJ8-ST7iL5ADr2LVhSrckUmoa8dx46ZzbEJsKsO4hEe0s-NjqiK53YBbTgO_K5a8NSp1c6mr7R7UZgKw1xFXwE0gVy5uaL9ER35K2DbqNzuXD6R2icxa-sCx4eJw9q3UR4snsPky8n7y6nZ-ns4vR8OpmlRtKiS01lBC8ElLJkttClqGTFoLbcogUPpbyqZAaaZ7WuJBtzg_5cSFlYU-SlOEyOt7qrvlqCNVhDqxu1at1St2sVtFN3Pd4t1DzcKMFFLuUYBV7tBNrwrYfYqaWLBqvWHkIfVYlN55JnDMnn_5DXoW89VocQ5kJ5Mci92EJz3YByvg74qxkk1YTnucyzgg5Zp3uoYZCYInavdmi-wx_t4fFaWDqzN-D1nQBkOvjezXUfoypOZ_9LZsea0DQwB4Xjml7s1TZtiLGF-rbbjKphtdWw2mpYbWSf_T2eW_L3LiPwcgfgxummbrU3Lv7hxllRsDwXvwDqf_76</recordid><startdate>20120101</startdate><enddate>20120101</enddate><creator>TCHOUKALOVA, Yourka D</creator><creator>FITCH, Mark</creator><creator>ROGERS, Pamela M</creator><creator>COVINGTON, Jeffrey D</creator><creator>HENAGAN, Tara M</creator><creator>JIANPING YE</creator><creator>HELLERSTEIN, Marc K</creator><creator>RAVUSSIN, Eric</creator><general>American Diabetes Association</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8GL</scope><scope>3V.</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88I</scope><scope>8AF</scope><scope>8AO</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BEC</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>HCIFZ</scope><scope>K9-</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0R</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M2P</scope><scope>M7P</scope><scope>MBDVC</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>S0X</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20120101</creationdate><title>In Vivo Adipogenesis in Rats Measured by Cell Kinetics in Adipocytes and Plastic-Adherent Stroma-Vascular Cells in Response to High-Fat Diet and Thiazolidinedione</title><author>TCHOUKALOVA, Yourka D ; FITCH, Mark ; ROGERS, Pamela M ; COVINGTON, Jeffrey D ; HENAGAN, Tara M ; JIANPING YE ; HELLERSTEIN, Marc K ; RAVUSSIN, Eric</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c608t-cbc3283e9691d8a93b6b1efd2d969999002bb64ea24fab6152cb1e73668dc8793</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Adipocytes</topic><topic>Adipocytes - drug effects</topic><topic>Adipocytes - physiology</topic><topic>Adipogenesis - drug effects</topic><topic>Adipogenesis - physiology</topic><topic>Adipose tissue</topic><topic>Adipose tissues</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Body fat</topic><topic>Bone marrow</topic><topic>Calories</topic><topic>Cell Adhesion</topic><topic>Cell Culture Techniques - instrumentation</topic><topic>Cell Differentiation - drug effects</topic><topic>Cell Differentiation - physiology</topic><topic>Cells, Cultured</topic><topic>Development and progression</topic><topic>Diabetes. Impaired glucose tolerance</topic><topic>Diet</topic><topic>Diet, High-Fat</topic><topic>Dietary Fats - pharmacology</topic><topic>Endocrine pancreas. Apud cells (diseases)</topic><topic>Endocrinopathies</topic><topic>Endothelial Cells - cytology</topic><topic>Endothelial Cells - drug effects</topic><topic>Endothelial Cells - physiology</topic><topic>Etiopathogenesis. Screening. Investigations. Target tissue resistance</topic><topic>Fat cells</topic><topic>Intervention</topic><topic>Kinetics</topic><topic>Laboratories</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Obesity</topic><topic>Obesity Studies</topic><topic>Physiological aspects</topic><topic>Plastics</topic><topic>Rats</topic><topic>Rats, Long-Evans</topic><topic>Stem cells</topic><topic>Stromal Cells - cytology</topic><topic>Stromal Cells - drug effects</topic><topic>Stromal Cells - physiology</topic><topic>Thiazolidinediones - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>TCHOUKALOVA, Yourka D</creatorcontrib><creatorcontrib>FITCH, Mark</creatorcontrib><creatorcontrib>ROGERS, Pamela M</creatorcontrib><creatorcontrib>COVINGTON, Jeffrey D</creatorcontrib><creatorcontrib>HENAGAN, Tara M</creatorcontrib><creatorcontrib>JIANPING YE</creatorcontrib><creatorcontrib>HELLERSTEIN, Marc K</creatorcontrib><creatorcontrib>RAVUSSIN, Eric</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: High School</collection><collection>ProQuest Central (Corporate)</collection><collection>ProQuest Nursing & Allied Health Database</collection><collection>Health & Medicine (ProQuest)</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>STEM Database</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest Public Health Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>eLibrary</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>SciTech Premium Collection</collection><collection>Consumer Health Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Biological Sciences</collection><collection>ProQuest Consumer Health Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>ProQuest Research Library</collection><collection>ProQuest Science Journals</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>SIRS Editorial</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Diabetes (New York, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>TCHOUKALOVA, Yourka D</au><au>FITCH, Mark</au><au>ROGERS, Pamela M</au><au>COVINGTON, Jeffrey D</au><au>HENAGAN, Tara M</au><au>JIANPING YE</au><au>HELLERSTEIN, Marc K</au><au>RAVUSSIN, Eric</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In Vivo Adipogenesis in Rats Measured by Cell Kinetics in Adipocytes and Plastic-Adherent Stroma-Vascular Cells in Response to High-Fat Diet and Thiazolidinedione</atitle><jtitle>Diabetes (New York, N.Y.)</jtitle><addtitle>Diabetes</addtitle><date>2012-01-01</date><risdate>2012</risdate><volume>61</volume><issue>1</issue><spage>137</spage><epage>144</epage><pages>137-144</pages><issn>0012-1797</issn><eissn>1939-327X</eissn><coden>DIAEAZ</coden><abstract>Impairment of adipogenesis contributes to the development of obesity-related insulin resistance. The current in vitro approaches for its assessment represent crude estimates of the adipogenic potential because of the disruption of the in vivo microenvironment. A novel assessment of in vivo adipogenesis using the incorporation of the stable isotope deuterium ((2)H) into the DNA of isolated adipocytes and stroma-vascular fraction from adipose tissue has been developed. In the current study, we have refined this technique by purifying the adipocytes via a negative immune selection and sorting the plastic adherent stroma-vascular (aSV) subfraction (using 3 h culture) that contains mostly adipocyte progenitor cells and ∼10% of small adipocytes. Using a 3-week 8% (2)H(2)O ingestion with a high-fat diet (HFD) or HFD plus pioglitazone (HFD-P), we demonstrate that the fractions of new aSV cells (f(aSV)) and immunopurified adipocytes (f(AD)) (the ratio of their (2)H-enrichment of DNA to the maximal (2)H-enrichment of DNA of bone marrow reference cells) recapitulate the known hyperplastic mechanism of weight gain with pioglitazone treatment. We conclude that f(aSV) and f(AD) are reliable indices of in vivo adipogenesis. The proposed method represents a valuable tool for studying the effect of interventions (drugs, diets, and exercise) on in vivo adipogenesis.</abstract><cop>Alexandria, VA</cop><pub>American Diabetes Association</pub><pmid>22124466</pmid><doi>10.2337/db10-1768</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Diabetes (New York, N.Y.), 2012-01, Vol.61 (1), p.137-144 |
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| subjects | Adipocytes Adipocytes - drug effects Adipocytes - physiology Adipogenesis - drug effects Adipogenesis - physiology Adipose tissue Adipose tissues Animals Biological and medical sciences Body fat Bone marrow Calories Cell Adhesion Cell Culture Techniques - instrumentation Cell Differentiation - drug effects Cell Differentiation - physiology Cells, Cultured Development and progression Diabetes. Impaired glucose tolerance Diet Diet, High-Fat Dietary Fats - pharmacology Endocrine pancreas. Apud cells (diseases) Endocrinopathies Endothelial Cells - cytology Endothelial Cells - drug effects Endothelial Cells - physiology Etiopathogenesis. Screening. Investigations. Target tissue resistance Fat cells Intervention Kinetics Laboratories Male Medical sciences Obesity Obesity Studies Physiological aspects Plastics Rats Rats, Long-Evans Stem cells Stromal Cells - cytology Stromal Cells - drug effects Stromal Cells - physiology Thiazolidinediones - pharmacology |
| title | In Vivo Adipogenesis in Rats Measured by Cell Kinetics in Adipocytes and Plastic-Adherent Stroma-Vascular Cells in Response to High-Fat Diet and Thiazolidinedione |
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