A Highly Dynamic ER-Derived Phosphatidylinositol-Synthesizing Organelle Supplies Phosphoinositides to Cellular Membranes
Polyphosphoinositides are lipid signaling molecules generated from phosphatidylinositol (PtdIns) with critical roles in vesicular trafficking and signaling. It is poorly understood where PtdIns is located within cells and how it moves around between membranes. Here we identify a hitherto-unrecognize...
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| Veröffentlicht in: | Developmental cell 2011-11, Vol.21 (5), p.813-824 |
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| creator | Kim, Yeun Ju Guzman-Hernandez, Maria Luisa Balla, Tamas |
| description | Polyphosphoinositides are lipid signaling molecules generated from phosphatidylinositol (PtdIns) with critical roles in vesicular trafficking and signaling. It is poorly understood where PtdIns is located within cells and how it moves around between membranes. Here we identify a hitherto-unrecognized highly mobile membrane compartment as the site of PtdIns synthesis and a likely source of PtdIns of all membranes. We show that the PtdIns-synthesizing enzyme PIS associates with a rapidly moving compartment of ER origin that makes ample contacts with other membranes. In contrast, CDP-diacylglycerol synthases that provide PIS with its substrate reside in the tubular ER. Expression of a PtdIns-specific bacterial PLC generates diacylglycerol also in rapidly moving cytoplasmic objects. We propose a model in which PtdIns is synthesized in a highly mobile lipid distribution platform and is delivered to other membranes during multiple contacts by yet-to-be-defined lipid transfer mechanisms.
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► PtdIns synthesis takes place in a highly mobile organelle ► The PtdIns-synthesizing organelle is ER derived ► The PtdIns-synthesizing organelle is the ultimate source of polyphosphoinositides ► PtdIns depletion at the plasma membrane but not the ER impacts PtdIns(4,5)P2 pools |
| doi_str_mv | 10.1016/j.devcel.2011.09.005 |
| format | Article |
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[Display omitted]
► PtdIns synthesis takes place in a highly mobile organelle ► The PtdIns-synthesizing organelle is ER derived ► The PtdIns-synthesizing organelle is the ultimate source of polyphosphoinositides ► PtdIns depletion at the plasma membrane but not the ER impacts PtdIns(4,5)P2 pools</description><identifier>ISSN: 1534-5807</identifier><identifier>ISSN: 1878-1551</identifier><identifier>EISSN: 1878-1551</identifier><identifier>DOI: 10.1016/j.devcel.2011.09.005</identifier><identifier>PMID: 22075145</identifier><language>eng</language><publisher>Cambridge, MA: Elsevier Inc</publisher><subject>Animals ; Biological and medical sciences ; Cell differentiation, maturation, development, hematopoiesis ; Cell Membrane - chemistry ; Cell Membrane - metabolism ; Cell physiology ; Chlorocebus aethiops ; COS Cells ; Diacylglycerol Cholinephosphotransferase - chemistry ; Diacylglycerol Cholinephosphotransferase - metabolism ; Endoplasmic Reticulum - chemistry ; Endoplasmic Reticulum - enzymology ; Endoplasmic Reticulum - metabolism ; Fundamental and applied biological sciences. Psychology ; HEK293 Cells ; Humans ; Molecular and cellular biology ; Organelles - chemistry ; Organelles - metabolism ; Phosphatidylinositols - biosynthesis ; Phosphatidylinositols - chemistry ; Phosphatidylinositols - metabolism ; Signal Transduction</subject><ispartof>Developmental cell, 2011-11, Vol.21 (5), p.813-824</ispartof><rights>2011 Elsevier Inc.</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2011 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c558t-b34e015590114d060ebef886be0bc39b1173d8ac17de550a76862822849b4cb93</citedby><cites>FETCH-LOGICAL-c558t-b34e015590114d060ebef886be0bc39b1173d8ac17de550a76862822849b4cb93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1534580711004035$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>230,314,776,780,881,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=25229727$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22075145$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kim, Yeun Ju</creatorcontrib><creatorcontrib>Guzman-Hernandez, Maria Luisa</creatorcontrib><creatorcontrib>Balla, Tamas</creatorcontrib><title>A Highly Dynamic ER-Derived Phosphatidylinositol-Synthesizing Organelle Supplies Phosphoinositides to Cellular Membranes</title><title>Developmental cell</title><addtitle>Dev Cell</addtitle><description>Polyphosphoinositides are lipid signaling molecules generated from phosphatidylinositol (PtdIns) with critical roles in vesicular trafficking and signaling. It is poorly understood where PtdIns is located within cells and how it moves around between membranes. Here we identify a hitherto-unrecognized highly mobile membrane compartment as the site of PtdIns synthesis and a likely source of PtdIns of all membranes. We show that the PtdIns-synthesizing enzyme PIS associates with a rapidly moving compartment of ER origin that makes ample contacts with other membranes. In contrast, CDP-diacylglycerol synthases that provide PIS with its substrate reside in the tubular ER. Expression of a PtdIns-specific bacterial PLC generates diacylglycerol also in rapidly moving cytoplasmic objects. We propose a model in which PtdIns is synthesized in a highly mobile lipid distribution platform and is delivered to other membranes during multiple contacts by yet-to-be-defined lipid transfer mechanisms.
[Display omitted]
► PtdIns synthesis takes place in a highly mobile organelle ► The PtdIns-synthesizing organelle is ER derived ► The PtdIns-synthesizing organelle is the ultimate source of polyphosphoinositides ► PtdIns depletion at the plasma membrane but not the ER impacts PtdIns(4,5)P2 pools</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cell differentiation, maturation, development, hematopoiesis</subject><subject>Cell Membrane - chemistry</subject><subject>Cell Membrane - metabolism</subject><subject>Cell physiology</subject><subject>Chlorocebus aethiops</subject><subject>COS Cells</subject><subject>Diacylglycerol Cholinephosphotransferase - chemistry</subject><subject>Diacylglycerol Cholinephosphotransferase - metabolism</subject><subject>Endoplasmic Reticulum - chemistry</subject><subject>Endoplasmic Reticulum - enzymology</subject><subject>Endoplasmic Reticulum - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>HEK293 Cells</subject><subject>Humans</subject><subject>Molecular and cellular biology</subject><subject>Organelles - chemistry</subject><subject>Organelles - metabolism</subject><subject>Phosphatidylinositols - biosynthesis</subject><subject>Phosphatidylinositols - chemistry</subject><subject>Phosphatidylinositols - metabolism</subject><subject>Signal Transduction</subject><issn>1534-5807</issn><issn>1878-1551</issn><issn>1878-1551</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU9v1DAQxS0EomXhGyCUC-opwX_i2LkgVdvSIhUVUThbjjO78cqJg52sCJ--rnZp4cLJlv17M_PmIfSW4IJgUn3YFS3sDbiCYkIKXBcY82folEghc8I5eZ7unJU5l1icoFcx7nCSEYlfohNKseCk5Kfo13l2bbedW7KLZdC9Ndnlt_wCgt1Dm33tfBw7Pdl2cXbw0U7e5XfLMHUQ7W87bLPbsNUDOAfZ3TyOzkI8ivyBt216mXy2TszsdMi-QN-EJImv0YuNdhHeHM8V-vHp8vv6Or-5vfq8Pr_JDedyyhtWAk526mSybHGFoYGNlFUDuDGsbggRrJXaENEC51iLSlZUUirLuilNU7MV-nioO85ND62BYQraqTHYXodFeW3Vvz-D7dTW7xWjjAsmUoGzY4Hgf84QJ9XbmPbukgs_R1XjEhPGEr5C5YE0wccYYPPYhWD1kJnaqUNm6iEzhWuVMkuyd39P-Cj6E1IC3h8BHY12m7Q_Y-MTxymtBRVPViHtc28hqGgsDAZaG8BMqvX2_5PcA_87ufQ</recordid><startdate>20111115</startdate><enddate>20111115</enddate><creator>Kim, Yeun Ju</creator><creator>Guzman-Hernandez, Maria Luisa</creator><creator>Balla, Tamas</creator><general>Elsevier Inc</general><general>Cell Press</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20111115</creationdate><title>A Highly Dynamic ER-Derived Phosphatidylinositol-Synthesizing Organelle Supplies Phosphoinositides to Cellular Membranes</title><author>Kim, Yeun Ju ; Guzman-Hernandez, Maria Luisa ; Balla, Tamas</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c558t-b34e015590114d060ebef886be0bc39b1173d8ac17de550a76862822849b4cb93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cell differentiation, maturation, development, hematopoiesis</topic><topic>Cell Membrane - chemistry</topic><topic>Cell Membrane - metabolism</topic><topic>Cell physiology</topic><topic>Chlorocebus aethiops</topic><topic>COS Cells</topic><topic>Diacylglycerol Cholinephosphotransferase - chemistry</topic><topic>Diacylglycerol Cholinephosphotransferase - metabolism</topic><topic>Endoplasmic Reticulum - chemistry</topic><topic>Endoplasmic Reticulum - enzymology</topic><topic>Endoplasmic Reticulum - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>HEK293 Cells</topic><topic>Humans</topic><topic>Molecular and cellular biology</topic><topic>Organelles - chemistry</topic><topic>Organelles - metabolism</topic><topic>Phosphatidylinositols - biosynthesis</topic><topic>Phosphatidylinositols - chemistry</topic><topic>Phosphatidylinositols - metabolism</topic><topic>Signal Transduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kim, Yeun Ju</creatorcontrib><creatorcontrib>Guzman-Hernandez, Maria Luisa</creatorcontrib><creatorcontrib>Balla, Tamas</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Developmental cell</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kim, Yeun Ju</au><au>Guzman-Hernandez, Maria Luisa</au><au>Balla, Tamas</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A Highly Dynamic ER-Derived Phosphatidylinositol-Synthesizing Organelle Supplies Phosphoinositides to Cellular Membranes</atitle><jtitle>Developmental cell</jtitle><addtitle>Dev Cell</addtitle><date>2011-11-15</date><risdate>2011</risdate><volume>21</volume><issue>5</issue><spage>813</spage><epage>824</epage><pages>813-824</pages><issn>1534-5807</issn><issn>1878-1551</issn><eissn>1878-1551</eissn><abstract>Polyphosphoinositides are lipid signaling molecules generated from phosphatidylinositol (PtdIns) with critical roles in vesicular trafficking and signaling. It is poorly understood where PtdIns is located within cells and how it moves around between membranes. Here we identify a hitherto-unrecognized highly mobile membrane compartment as the site of PtdIns synthesis and a likely source of PtdIns of all membranes. We show that the PtdIns-synthesizing enzyme PIS associates with a rapidly moving compartment of ER origin that makes ample contacts with other membranes. In contrast, CDP-diacylglycerol synthases that provide PIS with its substrate reside in the tubular ER. Expression of a PtdIns-specific bacterial PLC generates diacylglycerol also in rapidly moving cytoplasmic objects. We propose a model in which PtdIns is synthesized in a highly mobile lipid distribution platform and is delivered to other membranes during multiple contacts by yet-to-be-defined lipid transfer mechanisms.
[Display omitted]
► PtdIns synthesis takes place in a highly mobile organelle ► The PtdIns-synthesizing organelle is ER derived ► The PtdIns-synthesizing organelle is the ultimate source of polyphosphoinositides ► PtdIns depletion at the plasma membrane but not the ER impacts PtdIns(4,5)P2 pools</abstract><cop>Cambridge, MA</cop><pub>Elsevier Inc</pub><pmid>22075145</pmid><doi>10.1016/j.devcel.2011.09.005</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Biological and medical sciences Cell differentiation, maturation, development, hematopoiesis Cell Membrane - chemistry Cell Membrane - metabolism Cell physiology Chlorocebus aethiops COS Cells Diacylglycerol Cholinephosphotransferase - chemistry Diacylglycerol Cholinephosphotransferase - metabolism Endoplasmic Reticulum - chemistry Endoplasmic Reticulum - enzymology Endoplasmic Reticulum - metabolism Fundamental and applied biological sciences. Psychology HEK293 Cells Humans Molecular and cellular biology Organelles - chemistry Organelles - metabolism Phosphatidylinositols - biosynthesis Phosphatidylinositols - chemistry Phosphatidylinositols - metabolism Signal Transduction |
| title | A Highly Dynamic ER-Derived Phosphatidylinositol-Synthesizing Organelle Supplies Phosphoinositides to Cellular Membranes |
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