Azidohomoalanine: A Conformationally Sensitive IR Probe of Protein Folding, Protein Structure, and Electrostatics
Highly sensitive: The azido analogue of methionine, azidohomoalanine (see picture), is shown to be a sensitive IR probe of protein structure, folding, and electrostatics, as demonstrated for ribosomal protein NTL9. It can be readily incorporated in to proteins, and the azido frequency is significant...
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| Veröffentlicht in: | Angewandte Chemie (International ed.) 2010-10, Vol.49 (41), p.7473-7475 |
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| container_issue | 41 |
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| container_title | Angewandte Chemie (International ed.) |
| container_volume | 49 |
| creator | Taskent-Sezgin, Humeyra Chung, Juah Banerjee, Partha S Nagarajan, Sureshbabu Dyer, R. Brian Carrico, Isaac Raleigh, Daniel P |
| description | Highly sensitive: The azido analogue of methionine, azidohomoalanine (see picture), is shown to be a sensitive IR probe of protein structure, folding, and electrostatics, as demonstrated for ribosomal protein NTL9. It can be readily incorporated in to proteins, and the azido frequency is significantly blue‐shifted in the thermally unfolded state. |
| doi_str_mv | 10.1002/anie.201003325 |
| format | Article |
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| identifier | ISSN: 1433-7851 |
| ispartof | Angewandte Chemie (International ed.), 2010-10, Vol.49 (41), p.7473-7475 |
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| language | eng |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Alanine - analogs & derivatives Alanine - chemistry azidohomoalanine electrostatics infrared spectroscopy Models, Molecular Protein Conformation Protein Folding protein modifications Protein Structure, Tertiary Proteins - chemistry Ribosomal Proteins - chemistry Spectrophotometry, Infrared - methods Static Electricity |
| title | Azidohomoalanine: A Conformationally Sensitive IR Probe of Protein Folding, Protein Structure, and Electrostatics |
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