Cloning of the human glucocorticoid receptor cDNA
We show that the human glucocorticoid receptor (GR), isolated from the breast cancer cell line MCF-7, has an apparent molecular weight identical to that of rat liver GR (94 kDa) and reacts with antibodies raised against the latter. These antibodies were used to clone cDNA sequences corresponding to...
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| Veröffentlicht in: | Nucleic acids research 1985-12, Vol.13 (23), p.8293-8304 |
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| container_title | Nucleic acids research |
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| creator | Govindan, Manjapra Variath Devic, Martine Green, Stephen Gronemeyer, Hinrich Chambon, P. |
| description | We show that the human glucocorticoid receptor (GR), isolated from the breast cancer cell line MCF-7, has an apparent molecular weight identical to that of rat liver GR (94 kDa) and reacts with antibodies raised against the latter. These antibodies were used to clone cDNA sequences corresponding to the human GR from a λgt11 expression library constructed using MCF-7 poly(A)+ RNA. Three non-homologous cONA clones with Inserts of 125, 220 and 350 bp, which express epitopes recognised by the rat liver GR antibodies, were isolated. Rat liver GR antibodies, immunopurified using the immobilised purified β-galactos1dase fusion proteins, detect partially purified rat liver and human GRs on Western blots. In addition, these antibodies immuno-adsorb rat liver and human GRs affinity-labelled with [3H] triamcinolone acetonide. Northern blot analysis, using all three cDNA probes, reveals the presence of a major MCF-7 poly(A)+ RNA species of approximately 7 kb. |
| doi_str_mv | 10.1093/nar/13.23.8293 |
| format | Article |
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These antibodies were used to clone cDNA sequences corresponding to the human GR from a λgt11 expression library constructed using MCF-7 poly(A)+ RNA. Three non-homologous cONA clones with Inserts of 125, 220 and 350 bp, which express epitopes recognised by the rat liver GR antibodies, were isolated. Rat liver GR antibodies, immunopurified using the immobilised purified β-galactos1dase fusion proteins, detect partially purified rat liver and human GRs on Western blots. In addition, these antibodies immuno-adsorb rat liver and human GRs affinity-labelled with [3H] triamcinolone acetonide. Northern blot analysis, using all three cDNA probes, reveals the presence of a major MCF-7 poly(A)+ RNA species of approximately 7 kb.</description><identifier>ISSN: 0305-1048</identifier><identifier>EISSN: 1362-4962</identifier><identifier>DOI: 10.1093/nar/13.23.8293</identifier><identifier>PMID: 2417195</identifier><identifier>CODEN: NARHAD</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>Biological and medical sciences ; Biotechnology ; Cloning, Molecular ; DNA - genetics ; Fundamental and applied biological sciences. Psychology ; Genetic engineering ; Genetic technics ; Humans ; Methods. Procedures. 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These antibodies were used to clone cDNA sequences corresponding to the human GR from a λgt11 expression library constructed using MCF-7 poly(A)+ RNA. Three non-homologous cONA clones with Inserts of 125, 220 and 350 bp, which express epitopes recognised by the rat liver GR antibodies, were isolated. Rat liver GR antibodies, immunopurified using the immobilised purified β-galactos1dase fusion proteins, detect partially purified rat liver and human GRs on Western blots. In addition, these antibodies immuno-adsorb rat liver and human GRs affinity-labelled with [3H] triamcinolone acetonide. Northern blot analysis, using all three cDNA probes, reveals the presence of a major MCF-7 poly(A)+ RNA species of approximately 7 kb.</description><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Cloning, Molecular</subject><subject>DNA - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetic engineering</subject><subject>Genetic technics</subject><subject>Humans</subject><subject>Methods. Procedures. Technologies</subject><subject>Poly A - genetics</subject><subject>Receptors, Glucocorticoid - genetics</subject><subject>RNA - genetics</subject><subject>RNA, Messenger</subject><subject>Transcription, Genetic</subject><issn>0305-1048</issn><issn>1362-4962</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1PGzEQxa0KRFPaa2-V9oB62-Dx-PPQAwotIBD00EqoF8txvIlhsw72blX--y5KFLUnLjOH93ujmXmEfAQ6BWrwtHP5FHDKcKqZwTdkAihZzY1kB2RCkYoaKNdvybtSHigFDoIfkSPGQYEREwKzNnWxW1apqfpVqFbD2nXVsh188in30ae4qHLwYdOnXPnz27P35LBxbQkfdv2Y_Pz29cfssr65u7iand3UnmvW14YqpRqQc6qFAcBGoGZUCs-kWjiKzs1ZEwxnihnDw0KBbyijwXvUWo71mHzZzt0M83VY-ND12bV2k-Pa5WebXLT_K11c2WX6bZExQDH6P-_8OT0NofR2HYsPbeu6kIZilRQoBX0dBIOSG6NHcLoFfU6l5NDslwFqX8KwYxgW0DK0L2GMhk__nrDHd98f9ZOd7op3bZNd52PZY1pTwaUcsXqLxdKHP3vZ5UcrFSphL-9_2WvzHWf3yOw5_gVgwKBO</recordid><startdate>19851209</startdate><enddate>19851209</enddate><creator>Govindan, Manjapra Variath</creator><creator>Devic, Martine</creator><creator>Green, Stephen</creator><creator>Gronemeyer, Hinrich</creator><creator>Chambon, P.</creator><general>Oxford University Press</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19851209</creationdate><title>Cloning of the human glucocorticoid receptor cDNA</title><author>Govindan, Manjapra Variath ; Devic, Martine ; Green, Stephen ; Gronemeyer, Hinrich ; Chambon, P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c482t-90777f16b0859113f5382065c267da03aab2fe94272994ed71cf020ecc3886cc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1985</creationdate><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Cloning, Molecular</topic><topic>DNA - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetic engineering</topic><topic>Genetic technics</topic><topic>Humans</topic><topic>Methods. Procedures. Technologies</topic><topic>Poly A - genetics</topic><topic>Receptors, Glucocorticoid - genetics</topic><topic>RNA - genetics</topic><topic>RNA, Messenger</topic><topic>Transcription, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Govindan, Manjapra Variath</creatorcontrib><creatorcontrib>Devic, Martine</creatorcontrib><creatorcontrib>Green, Stephen</creatorcontrib><creatorcontrib>Gronemeyer, Hinrich</creatorcontrib><creatorcontrib>Chambon, P.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Nucleic acids research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Govindan, Manjapra Variath</au><au>Devic, Martine</au><au>Green, Stephen</au><au>Gronemeyer, Hinrich</au><au>Chambon, P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cloning of the human glucocorticoid receptor cDNA</atitle><jtitle>Nucleic acids research</jtitle><addtitle>Nucleic Acids Res</addtitle><date>1985-12-09</date><risdate>1985</risdate><volume>13</volume><issue>23</issue><spage>8293</spage><epage>8304</epage><pages>8293-8304</pages><issn>0305-1048</issn><eissn>1362-4962</eissn><coden>NARHAD</coden><abstract>We show that the human glucocorticoid receptor (GR), isolated from the breast cancer cell line MCF-7, has an apparent molecular weight identical to that of rat liver GR (94 kDa) and reacts with antibodies raised against the latter. These antibodies were used to clone cDNA sequences corresponding to the human GR from a λgt11 expression library constructed using MCF-7 poly(A)+ RNA. Three non-homologous cONA clones with Inserts of 125, 220 and 350 bp, which express epitopes recognised by the rat liver GR antibodies, were isolated. Rat liver GR antibodies, immunopurified using the immobilised purified β-galactos1dase fusion proteins, detect partially purified rat liver and human GRs on Western blots. In addition, these antibodies immuno-adsorb rat liver and human GRs affinity-labelled with [3H] triamcinolone acetonide. Northern blot analysis, using all three cDNA probes, reveals the presence of a major MCF-7 poly(A)+ RNA species of approximately 7 kb.</abstract><cop>Oxford</cop><pub>Oxford University Press</pub><pmid>2417195</pmid><doi>10.1093/nar/13.23.8293</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Nucleic acids research, 1985-12, Vol.13 (23), p.8293-8304 |
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| source | MEDLINE; Oxford University Press Journals Digital Archive Legacy; PubMed Central |
| subjects | Biological and medical sciences Biotechnology Cloning, Molecular DNA - genetics Fundamental and applied biological sciences. Psychology Genetic engineering Genetic technics Humans Methods. Procedures. Technologies Poly A - genetics Receptors, Glucocorticoid - genetics RNA - genetics RNA, Messenger Transcription, Genetic |
| title | Cloning of the human glucocorticoid receptor cDNA |
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