β-ionone activates and bleaches visual pigment in salamander photoreceptors
Vision begins with photoisomerization of 11-cis retinal to the all-trans conformation within the chromophore-binding pocket of opsin, leading to activation of a biochemical cascade. Release of all-trans retinal from the binding pocket curtails but does not fully quench the ability of opsin to activa...
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| Veröffentlicht in: | Visual neuroscience 2009-05, Vol.26 (3), p.267-274 |
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| creator | ISAYAMA, TOMOKI McCABE ENGLAND, S.L. CROUCH, R.K. ZIMMERMAN, A.L. MAKINO, C.L. |
| description | Vision begins with photoisomerization of 11-cis retinal to the all-trans conformation within the chromophore-binding pocket of opsin, leading to activation of a biochemical cascade. Release of all-trans retinal from the binding pocket curtails but does not fully quench the ability of opsin to activate transducin. All-trans retinal and some other analogs, such as β-ionone, enhance opsin’s activity, presumably on binding the empty chromophore-binding pocket. By recording from isolated salamander photoreceptors and from patches of rod outer segment membrane, we now show that high concentrations of β-ionone suppressed circulating current in dark-adapted green-sensitive rods by inhibiting the cyclic nucleotide-gated channels. There were also decreases in circulating current and flash sensitivity, and accelerated flash response kinetics in dark-adapted blue-sensitive (BS) rods and cones, and in ultraviolet-sensitive cones, at concentrations too low to inhibit the channels. These effects persisted in BS rods even after incubation with 9-cis retinal to ensure complete regeneration of their visual pigment. After long exposures to high concentrations of β-ionone, recovery was incomplete unless 9-cis retinal was given, indicating that visual pigment had been bleached. Therefore, we propose that β-ionone activates and bleaches some types of visual pigments, mimicking the effects of light. |
| doi_str_mv | 10.1017/S0952523809090105 |
| format | Article |
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Release of all-trans retinal from the binding pocket curtails but does not fully quench the ability of opsin to activate transducin. All-trans retinal and some other analogs, such as β-ionone, enhance opsin’s activity, presumably on binding the empty chromophore-binding pocket. By recording from isolated salamander photoreceptors and from patches of rod outer segment membrane, we now show that high concentrations of β-ionone suppressed circulating current in dark-adapted green-sensitive rods by inhibiting the cyclic nucleotide-gated channels. There were also decreases in circulating current and flash sensitivity, and accelerated flash response kinetics in dark-adapted blue-sensitive (BS) rods and cones, and in ultraviolet-sensitive cones, at concentrations too low to inhibit the channels. These effects persisted in BS rods even after incubation with 9-cis retinal to ensure complete regeneration of their visual pigment. 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Psychology ; In Vitro Techniques ; Kinetics ; Larva ; Membranes - drug effects ; Membranes - metabolism ; Membranes - physiology ; Neurophysiology ; Norisoprenoids - administration & dosage ; Norisoprenoids - pharmacology ; Photoreceptor Cells, Vertebrate - drug effects ; Photoreceptor Cells, Vertebrate - metabolism ; Photoreceptor Cells, Vertebrate - physiology ; Phototransduction ; Retinal Pigments - metabolism ; Retinal Rod Photoreceptor Cells - drug effects ; Retinal Rod Photoreceptor Cells - physiology ; Retinoids ; Rhodopsin ; Rod Cell Outer Segment - drug effects ; Rod Cell Outer Segment - metabolism ; Rod Cell Outer Segment - physiology ; Time Factors ; Urodela - growth & development ; Urodela - metabolism ; Vertebrates: nervous system and sense organs</subject><ispartof>Visual neuroscience, 2009-05, Vol.26 (3), p.267-274</ispartof><rights>Copyright © Cambridge University Press 2009</rights><rights>2009 INIST-CNRS</rights><rights>Copyright © 2009 Cambridge University Press 2009</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c540t-49635aabca244d65bc26b3172559a3097eb44a938f8220620928944d1d7b1f0d3</citedby><cites>FETCH-LOGICAL-c540t-49635aabca244d65bc26b3172559a3097eb44a938f8220620928944d1d7b1f0d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.cambridge.org/core/product/identifier/S0952523809090105/type/journal_article$$EHTML$$P50$$Gcambridge$$H</linktohtml><link.rule.ids>164,230,314,776,780,881,27901,27902,55603</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21820048$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19500430$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>ISAYAMA, TOMOKI</creatorcontrib><creatorcontrib>McCABE ENGLAND, S.L.</creatorcontrib><creatorcontrib>CROUCH, R.K.</creatorcontrib><creatorcontrib>ZIMMERMAN, A.L.</creatorcontrib><creatorcontrib>MAKINO, C.L.</creatorcontrib><title>β-ionone activates and bleaches visual pigment in salamander photoreceptors</title><title>Visual neuroscience</title><addtitle>Vis Neurosci</addtitle><description>Vision begins with photoisomerization of 11-cis retinal to the all-trans conformation within the chromophore-binding pocket of opsin, leading to activation of a biochemical cascade. Release of all-trans retinal from the binding pocket curtails but does not fully quench the ability of opsin to activate transducin. All-trans retinal and some other analogs, such as β-ionone, enhance opsin’s activity, presumably on binding the empty chromophore-binding pocket. By recording from isolated salamander photoreceptors and from patches of rod outer segment membrane, we now show that high concentrations of β-ionone suppressed circulating current in dark-adapted green-sensitive rods by inhibiting the cyclic nucleotide-gated channels. There were also decreases in circulating current and flash sensitivity, and accelerated flash response kinetics in dark-adapted blue-sensitive (BS) rods and cones, and in ultraviolet-sensitive cones, at concentrations too low to inhibit the channels. These effects persisted in BS rods even after incubation with 9-cis retinal to ensure complete regeneration of their visual pigment. After long exposures to high concentrations of β-ionone, recovery was incomplete unless 9-cis retinal was given, indicating that visual pigment had been bleached. Therefore, we propose that β-ionone activates and bleaches some types of visual pigments, mimicking the effects of light.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Caudata</subject><subject>Cyclic Nucleotide-Gated Cation Channels - antagonists & inhibitors</subject><subject>Cyclic nucleotide-gated channel</subject><subject>Dark Adaptation - physiology</subject><subject>Dose-Response Relationship, Drug</subject><subject>Electric Conductivity</subject><subject>Eye and associated structures. Visual pathways and centers. Vision</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>In Vitro Techniques</subject><subject>Kinetics</subject><subject>Larva</subject><subject>Membranes - drug effects</subject><subject>Membranes - metabolism</subject><subject>Membranes - physiology</subject><subject>Neurophysiology</subject><subject>Norisoprenoids - administration & dosage</subject><subject>Norisoprenoids - pharmacology</subject><subject>Photoreceptor Cells, Vertebrate - drug effects</subject><subject>Photoreceptor Cells, Vertebrate - metabolism</subject><subject>Photoreceptor Cells, Vertebrate - physiology</subject><subject>Phototransduction</subject><subject>Retinal Pigments - metabolism</subject><subject>Retinal Rod Photoreceptor Cells - drug effects</subject><subject>Retinal Rod Photoreceptor Cells - physiology</subject><subject>Retinoids</subject><subject>Rhodopsin</subject><subject>Rod Cell Outer Segment - drug effects</subject><subject>Rod Cell Outer Segment - metabolism</subject><subject>Rod Cell Outer Segment - physiology</subject><subject>Time Factors</subject><subject>Urodela - growth & development</subject><subject>Urodela - metabolism</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0952-5238</issn><issn>1469-8714</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kc1u1DAUhS1ERaeFB2CDsoFdwL9xvEFCVWmRRhQErK0bx5lxSexgJyN4LR6kz1SPJhpASJUXV9b57vGRD0LPCX5NMJFvvmAlqKCsxiofgsUjtCK8UmUtCX-MVnu53Oun6CylW4wJI4I9QadECYw5wyu0vvtduuCDtwWYye1gsqkA3xZNb8Fs82Xn0gx9MbrNYP1UOF8k6GHIjI3FuA1TiNbYMY_0FJ100Cf7bJnn6Nv7y68X1-X65urDxbt1aQTHU8lVxQRAY4By3laiMbRqGJFUCAUMK2kbzkGxuqspxRXFitYqk6SVDelwy87R24PvODeDbU3OFaHXY3QDxF86gNP_Kt5t9SbsNMt-VLJs8GoxiOHHbNOkB5eM7XvwNsxJUywxU0JlkBxAE0NK0XbHRwjW-w70fx3knRd_p_uzsXx6Bl4uACQDfRfBG5eOHCU1zWCdufLAuTTZn0cd4nddSSaFrq4-64-MSvLputbrzLMlLAxNdO3G6tswR5-reCDuPXyJrek</recordid><startdate>20090501</startdate><enddate>20090501</enddate><creator>ISAYAMA, TOMOKI</creator><creator>McCABE ENGLAND, S.L.</creator><creator>CROUCH, R.K.</creator><creator>ZIMMERMAN, A.L.</creator><creator>MAKINO, C.L.</creator><general>Cambridge University Press</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>5PM</scope></search><sort><creationdate>20090501</creationdate><title>β-ionone activates and bleaches visual pigment in salamander photoreceptors</title><author>ISAYAMA, TOMOKI ; McCABE ENGLAND, S.L. ; CROUCH, R.K. ; ZIMMERMAN, A.L. ; MAKINO, C.L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c540t-49635aabca244d65bc26b3172559a3097eb44a938f8220620928944d1d7b1f0d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Caudata</topic><topic>Cyclic Nucleotide-Gated Cation Channels - antagonists & inhibitors</topic><topic>Cyclic nucleotide-gated channel</topic><topic>Dark Adaptation - physiology</topic><topic>Dose-Response Relationship, Drug</topic><topic>Electric Conductivity</topic><topic>Eye and associated structures. Visual pathways and centers. Vision</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>In Vitro Techniques</topic><topic>Kinetics</topic><topic>Larva</topic><topic>Membranes - drug effects</topic><topic>Membranes - metabolism</topic><topic>Membranes - physiology</topic><topic>Neurophysiology</topic><topic>Norisoprenoids - administration & dosage</topic><topic>Norisoprenoids - pharmacology</topic><topic>Photoreceptor Cells, Vertebrate - drug effects</topic><topic>Photoreceptor Cells, Vertebrate - metabolism</topic><topic>Photoreceptor Cells, Vertebrate - physiology</topic><topic>Phototransduction</topic><topic>Retinal Pigments - metabolism</topic><topic>Retinal Rod Photoreceptor Cells - drug effects</topic><topic>Retinal Rod Photoreceptor Cells - physiology</topic><topic>Retinoids</topic><topic>Rhodopsin</topic><topic>Rod Cell Outer Segment - drug effects</topic><topic>Rod Cell Outer Segment - metabolism</topic><topic>Rod Cell Outer Segment - physiology</topic><topic>Time Factors</topic><topic>Urodela - growth & development</topic><topic>Urodela - metabolism</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>ISAYAMA, TOMOKI</creatorcontrib><creatorcontrib>McCABE ENGLAND, S.L.</creatorcontrib><creatorcontrib>CROUCH, R.K.</creatorcontrib><creatorcontrib>ZIMMERMAN, A.L.</creatorcontrib><creatorcontrib>MAKINO, C.L.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Visual neuroscience</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>ISAYAMA, TOMOKI</au><au>McCABE ENGLAND, S.L.</au><au>CROUCH, R.K.</au><au>ZIMMERMAN, A.L.</au><au>MAKINO, C.L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>β-ionone activates and bleaches visual pigment in salamander photoreceptors</atitle><jtitle>Visual neuroscience</jtitle><addtitle>Vis Neurosci</addtitle><date>2009-05-01</date><risdate>2009</risdate><volume>26</volume><issue>3</issue><spage>267</spage><epage>274</epage><pages>267-274</pages><issn>0952-5238</issn><eissn>1469-8714</eissn><abstract>Vision begins with photoisomerization of 11-cis retinal to the all-trans conformation within the chromophore-binding pocket of opsin, leading to activation of a biochemical cascade. Release of all-trans retinal from the binding pocket curtails but does not fully quench the ability of opsin to activate transducin. All-trans retinal and some other analogs, such as β-ionone, enhance opsin’s activity, presumably on binding the empty chromophore-binding pocket. By recording from isolated salamander photoreceptors and from patches of rod outer segment membrane, we now show that high concentrations of β-ionone suppressed circulating current in dark-adapted green-sensitive rods by inhibiting the cyclic nucleotide-gated channels. There were also decreases in circulating current and flash sensitivity, and accelerated flash response kinetics in dark-adapted blue-sensitive (BS) rods and cones, and in ultraviolet-sensitive cones, at concentrations too low to inhibit the channels. These effects persisted in BS rods even after incubation with 9-cis retinal to ensure complete regeneration of their visual pigment. 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| ispartof | Visual neuroscience, 2009-05, Vol.26 (3), p.267-274 |
| issn | 0952-5238 1469-8714 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3220273 |
| source | MEDLINE; Cambridge University Press Journals Complete |
| subjects | Animals Biological and medical sciences Caudata Cyclic Nucleotide-Gated Cation Channels - antagonists & inhibitors Cyclic nucleotide-gated channel Dark Adaptation - physiology Dose-Response Relationship, Drug Electric Conductivity Eye and associated structures. Visual pathways and centers. Vision Fundamental and applied biological sciences. Psychology In Vitro Techniques Kinetics Larva Membranes - drug effects Membranes - metabolism Membranes - physiology Neurophysiology Norisoprenoids - administration & dosage Norisoprenoids - pharmacology Photoreceptor Cells, Vertebrate - drug effects Photoreceptor Cells, Vertebrate - metabolism Photoreceptor Cells, Vertebrate - physiology Phototransduction Retinal Pigments - metabolism Retinal Rod Photoreceptor Cells - drug effects Retinal Rod Photoreceptor Cells - physiology Retinoids Rhodopsin Rod Cell Outer Segment - drug effects Rod Cell Outer Segment - metabolism Rod Cell Outer Segment - physiology Time Factors Urodela - growth & development Urodela - metabolism Vertebrates: nervous system and sense organs |
| title | β-ionone activates and bleaches visual pigment in salamander photoreceptors |
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