Cloning and characterization of the gene for the yeast cytoplasmic threonyl-tRNA synthetase

A fragment of DNA from the yeast nuclear gene MST1 that codes for the mitochondrial tRNAThr1 synthetase was used as a probe to screen for other yeast threonyl-tRNA synthetase genes. At low stringency, the MST1 probe hybridizes strongly to a 6.6 kb EcoRl fragment of yeast genomic DNA with the homolog...

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Veröffentlicht in:	Nucleic acids research 1985-09, Vol.13 (17), p.6171-6183
Hauptverfasser:	Pape, Louis K., Tzagoloff, Alexander
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Amino Acyl-tRNA Synthetases - genetics Base Sequence Biological and medical sciences Biotechnology Cloning, Molecular DNA Restriction Enzymes Fundamental and applied biological sciences. Psychology Genes Genes, Fungal Genetic engineering Genetic technics Methods. Procedures. Technologies Molecular cloning Nucleic Acid Hybridization Saccharomyces cerevisiae - enzymology Saccharomyces cerevisiae - genetics Threonine-tRNA Ligase - genetics
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