Cloning and characterization of the gene for the yeast cytoplasmic threonyl-tRNA synthetase
A fragment of DNA from the yeast nuclear gene MST1 that codes for the mitochondrial tRNAThr1 synthetase was used as a probe to screen for other yeast threonyl-tRNA synthetase genes. At low stringency, the MST1 probe hybridizes strongly to a 6.6 kb EcoRl fragment of yeast genomic DNA with the homolog...
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| Veröffentlicht in: | Nucleic acids research 1985-09, Vol.13 (17), p.6171-6183 |
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| creator | Pape, Louis K. Tzagoloff, Alexander |
| description | A fragment of DNA from the yeast nuclear gene MST1 that codes for the mitochondrial tRNAThr1 synthetase was used as a probe to screen for other yeast threonyl-tRNA synthetase genes. At low stringency, the MST1 probe hybridizes strongly to a 6.6 kb EcoRl fragment of yeast genomic DNA with the homologous gene and in addition hybridizes more weakly to a smaller 3.6 kb EcoRl fragment with a second threonyl-tRNA synthetase gene (THS1). To clone THS1, a library was constructed by ligation to pUC18 of size selected (3–4.5 kb) EcoRI fragments of genomic DNA. Several clones containing the 3.6 kb EcoRI fragment were isolated. A 2,202 nucleotide long open reading frame corresponding to THS1 has been identified in the cloned fragment of DNA. The predicted protein encoded by THS1 is 38% identical to the E. coli threonyl-tRNA synthetase over the latter′s length (642 amino acids) and is 42% identical to the predicted MST1 product over its 462 residues. In situ disruption of the chromosomal copy of THS1 is lethal to die cell, indicating that this gene codes for the cytoplasmic threonyl-tRNA synthetase. |
| doi_str_mv | 10.1093/nar/13.17.6171 |
| format | Article |
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At low stringency, the MST1 probe hybridizes strongly to a 6.6 kb EcoRl fragment of yeast genomic DNA with the homologous gene and in addition hybridizes more weakly to a smaller 3.6 kb EcoRl fragment with a second threonyl-tRNA synthetase gene (THS1). To clone THS1, a library was constructed by ligation to pUC18 of size selected (3–4.5 kb) EcoRI fragments of genomic DNA. Several clones containing the 3.6 kb EcoRI fragment were isolated. A 2,202 nucleotide long open reading frame corresponding to THS1 has been identified in the cloned fragment of DNA. The predicted protein encoded by THS1 is 38% identical to the E. coli threonyl-tRNA synthetase over the latter′s length (642 amino acids) and is 42% identical to the predicted MST1 product over its 462 residues. In situ disruption of the chromosomal copy of THS1 is lethal to die cell, indicating that this gene codes for the cytoplasmic threonyl-tRNA synthetase.</description><identifier>ISSN: 0305-1048</identifier><identifier>EISSN: 1362-4962</identifier><identifier>DOI: 10.1093/nar/13.17.6171</identifier><identifier>PMID: 2995918</identifier><identifier>CODEN: NARHAD</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>Amino Acid Sequence ; Amino Acyl-tRNA Synthetases - genetics ; Base Sequence ; Biological and medical sciences ; Biotechnology ; Cloning, Molecular ; DNA Restriction Enzymes ; Fundamental and applied biological sciences. Psychology ; Genes ; Genes, Fungal ; Genetic engineering ; Genetic technics ; Methods. Procedures. Technologies ; Molecular cloning ; Nucleic Acid Hybridization ; Saccharomyces cerevisiae - enzymology ; Saccharomyces cerevisiae - genetics ; Threonine-tRNA Ligase - genetics</subject><ispartof>Nucleic acids research, 1985-09, Vol.13 (17), p.6171-6183</ispartof><rights>1986 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4281-15e9422b220deac907182ffc119c71abfcce2ee2d3c3620017a18873c00fe8f33</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC321945/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC321945/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8711440$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2995918$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pape, Louis K.</creatorcontrib><creatorcontrib>Tzagoloff, Alexander</creatorcontrib><title>Cloning and characterization of the gene for the yeast cytoplasmic threonyl-tRNA synthetase</title><title>Nucleic acids research</title><addtitle>Nucleic Acids Res</addtitle><description>A fragment of DNA from the yeast nuclear gene MST1 that codes for the mitochondrial tRNAThr1 synthetase was used as a probe to screen for other yeast threonyl-tRNA synthetase genes. At low stringency, the MST1 probe hybridizes strongly to a 6.6 kb EcoRl fragment of yeast genomic DNA with the homologous gene and in addition hybridizes more weakly to a smaller 3.6 kb EcoRl fragment with a second threonyl-tRNA synthetase gene (THS1). To clone THS1, a library was constructed by ligation to pUC18 of size selected (3–4.5 kb) EcoRI fragments of genomic DNA. Several clones containing the 3.6 kb EcoRI fragment were isolated. A 2,202 nucleotide long open reading frame corresponding to THS1 has been identified in the cloned fragment of DNA. The predicted protein encoded by THS1 is 38% identical to the E. coli threonyl-tRNA synthetase over the latter′s length (642 amino acids) and is 42% identical to the predicted MST1 product over its 462 residues. In situ disruption of the chromosomal copy of THS1 is lethal to die cell, indicating that this gene codes for the cytoplasmic threonyl-tRNA synthetase.</description><subject>Amino Acid Sequence</subject><subject>Amino Acyl-tRNA Synthetases - genetics</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Cloning, Molecular</subject><subject>DNA Restriction Enzymes</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes</subject><subject>Genes, Fungal</subject><subject>Genetic engineering</subject><subject>Genetic technics</subject><subject>Methods. Procedures. Technologies</subject><subject>Molecular cloning</subject><subject>Nucleic Acid Hybridization</subject><subject>Saccharomyces cerevisiae - enzymology</subject><subject>Saccharomyces cerevisiae - genetics</subject><subject>Threonine-tRNA Ligase - genetics</subject><issn>0305-1048</issn><issn>1362-4962</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUFvEzEQhS0EKqFw5Ya0B8RtU4_tXdsHDlVEKagqCAGq4GA5zjhZ2NjBdhDh1-OSKIJTT9b4fW80T4-Qp0CnQDU_CzadAZ-CnPYg4R6ZAO9ZK3TP7pMJ5bRrgQr1kDzK-RulIKATJ-SEad1pUBPydTbGMIRlY8OicSubrCuYht-2DDE00Tdlhc0SAzY-pr_DDm0ujduVuBltXg-u_iaMYTe25cP1eZN3oWLFZnxMHng7ZnxyeE_Jp4tXH2eX7dW7129m51etE0xBCx1qwdicMbpA6zSVoJj3DkA7CXbunUOGyBbc1Wg1g7SglOSOUo_Kc35KXu73brbzNS4chpLsaDZpWNu0M9EO5n8lDCuzjD8NZ6BFV_0vDv4Uf2wxF7MessNxtAHjNhvZc90xzu8EGWVUqZ7dCYLoO6o6UcHpHnQp5pzQH68Gam77NbVfA9yANLf9VsOzf7Me8UOhVX9-0G12dvTJBjfkI6YkgBC0Yu0eG3LBX0fZpu-ml1x25vLmi3k_-3yj315cG8n_AEXrvro</recordid><startdate>19850911</startdate><enddate>19850911</enddate><creator>Pape, Louis K.</creator><creator>Tzagoloff, Alexander</creator><general>Oxford University Press</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19850911</creationdate><title>Cloning and characterization of the gene for the yeast cytoplasmic threonyl-tRNA synthetase</title><author>Pape, Louis K. ; Tzagoloff, Alexander</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4281-15e9422b220deac907182ffc119c71abfcce2ee2d3c3620017a18873c00fe8f33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1985</creationdate><topic>Amino Acid Sequence</topic><topic>Amino Acyl-tRNA Synthetases - genetics</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Cloning, Molecular</topic><topic>DNA Restriction Enzymes</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes</topic><topic>Genes, Fungal</topic><topic>Genetic engineering</topic><topic>Genetic technics</topic><topic>Methods. Procedures. Technologies</topic><topic>Molecular cloning</topic><topic>Nucleic Acid Hybridization</topic><topic>Saccharomyces cerevisiae - enzymology</topic><topic>Saccharomyces cerevisiae - genetics</topic><topic>Threonine-tRNA Ligase - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pape, Louis K.</creatorcontrib><creatorcontrib>Tzagoloff, Alexander</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Nucleic acids research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pape, Louis K.</au><au>Tzagoloff, Alexander</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cloning and characterization of the gene for the yeast cytoplasmic threonyl-tRNA synthetase</atitle><jtitle>Nucleic acids research</jtitle><addtitle>Nucleic Acids Res</addtitle><date>1985-09-11</date><risdate>1985</risdate><volume>13</volume><issue>17</issue><spage>6171</spage><epage>6183</epage><pages>6171-6183</pages><issn>0305-1048</issn><eissn>1362-4962</eissn><coden>NARHAD</coden><abstract>A fragment of DNA from the yeast nuclear gene MST1 that codes for the mitochondrial tRNAThr1 synthetase was used as a probe to screen for other yeast threonyl-tRNA synthetase genes. At low stringency, the MST1 probe hybridizes strongly to a 6.6 kb EcoRl fragment of yeast genomic DNA with the homologous gene and in addition hybridizes more weakly to a smaller 3.6 kb EcoRl fragment with a second threonyl-tRNA synthetase gene (THS1). To clone THS1, a library was constructed by ligation to pUC18 of size selected (3–4.5 kb) EcoRI fragments of genomic DNA. Several clones containing the 3.6 kb EcoRI fragment were isolated. A 2,202 nucleotide long open reading frame corresponding to THS1 has been identified in the cloned fragment of DNA. The predicted protein encoded by THS1 is 38% identical to the E. coli threonyl-tRNA synthetase over the latter′s length (642 amino acids) and is 42% identical to the predicted MST1 product over its 462 residues. In situ disruption of the chromosomal copy of THS1 is lethal to die cell, indicating that this gene codes for the cytoplasmic threonyl-tRNA synthetase.</abstract><cop>Oxford</cop><pub>Oxford University Press</pub><pmid>2995918</pmid><doi>10.1093/nar/13.17.6171</doi><tpages>13</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Amino Acid Sequence Amino Acyl-tRNA Synthetases - genetics Base Sequence Biological and medical sciences Biotechnology Cloning, Molecular DNA Restriction Enzymes Fundamental and applied biological sciences. Psychology Genes Genes, Fungal Genetic engineering Genetic technics Methods. Procedures. Technologies Molecular cloning Nucleic Acid Hybridization Saccharomyces cerevisiae - enzymology Saccharomyces cerevisiae - genetics Threonine-tRNA Ligase - genetics |
| title | Cloning and characterization of the gene for the yeast cytoplasmic threonyl-tRNA synthetase |
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