A bidirectionally active signal for termination of transcription is located between tetA and orfL on transposon Tn10
A terminator of transcription with bidirectional activity has been located between the translation termination codons of the genes tetA and orfL on Tn 10. These genes are transcribed towards each other. Each orientation of the intervening sequence is shown to reduce the expression of the lacZ and ga...
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| Veröffentlicht in: | Nucleic acids research 1985-06, Vol.13 (12), p.4227-4237 |
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| container_title | Nucleic acids research |
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| creator | Schollmeier, Klaus Gärtner, Dagmar Hillen, Wolfgang |
| description | A terminator of transcription with bidirectional activity has been located between the translation termination codons of the genes tetA and orfL on Tn 10. These genes are transcribed towards each other. Each orientation of the intervening sequence is shown to reduce the expression of the lacZ and galK genes when cloned between the respective structural gene and its promotor. The 3′ends of the respective mRNAs were determined by S1 mapping. The results confirm that the same sequence capable of forming a stem-loop structure with a GC rich stem is the termination signal for both orientations. In the more efficient tetA orientation (99%–96% reduction of expression) this sequence is followed by a run of six thymines. In the less efficient orfL orientation (96%–78% reduction of expression) it is followed by an AT rich sequence with seven thymines out of eleven base pairs |
| doi_str_mv | 10.1093/nar/13.12.4227 |
| format | Article |
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These genes are transcribed towards each other. Each orientation of the intervening sequence is shown to reduce the expression of the lacZ and galK genes when cloned between the respective structural gene and its promotor. The 3′ends of the respective mRNAs were determined by S1 mapping. The results confirm that the same sequence capable of forming a stem-loop structure with a GC rich stem is the termination signal for both orientations. In the more efficient tetA orientation (99%–96% reduction of expression) this sequence is followed by a run of six thymines. In the less efficient orfL orientation (96%–78% reduction of expression) it is followed by an AT rich sequence with seven thymines out of eleven base pairs</description><identifier>ISSN: 0305-1048</identifier><identifier>EISSN: 1362-4962</identifier><identifier>DOI: 10.1093/nar/13.12.4227</identifier><identifier>PMID: 2989790</identifier><identifier>CODEN: NARHAD</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>Bacterial Proteins - genetics ; Base Sequence ; beta-Galactosidase - genetics ; Biological and medical sciences ; DNA Restriction Enzymes ; DNA Transposable Elements ; Endonucleases ; Escherichia coli - enzymology ; Escherichia coli - genetics ; Fundamental and applied biological sciences. Psychology ; Galactosidases - genetics ; Genes ; Genes, Bacterial ; Genes, Regulator ; Molecular and cellular biology ; Molecular genetics ; Nucleic Acid Conformation ; Plasmids ; Single-Strand Specific DNA and RNA Endonucleases ; Terminator Regions, Genetic ; Transcription, Genetic ; Transcription. Transcription factor. Splicing. Rna processing</subject><ispartof>Nucleic acids research, 1985-06, Vol.13 (12), p.4227-4237</ispartof><rights>1985 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC321784/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC321784/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=9281507$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2989790$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Schollmeier, Klaus</creatorcontrib><creatorcontrib>Gärtner, Dagmar</creatorcontrib><creatorcontrib>Hillen, Wolfgang</creatorcontrib><title>A bidirectionally active signal for termination of transcription is located between tetA and orfL on transposon Tn10</title><title>Nucleic acids research</title><addtitle>Nucleic Acids Res</addtitle><description>A terminator of transcription with bidirectional activity has been located between the translation termination codons of the genes tetA and orfL on Tn 10. These genes are transcribed towards each other. Each orientation of the intervening sequence is shown to reduce the expression of the lacZ and galK genes when cloned between the respective structural gene and its promotor. The 3′ends of the respective mRNAs were determined by S1 mapping. The results confirm that the same sequence capable of forming a stem-loop structure with a GC rich stem is the termination signal for both orientations. In the more efficient tetA orientation (99%–96% reduction of expression) this sequence is followed by a run of six thymines. In the less efficient orfL orientation (96%–78% reduction of expression) it is followed by an AT rich sequence with seven thymines out of eleven base pairs</description><subject>Bacterial Proteins - genetics</subject><subject>Base Sequence</subject><subject>beta-Galactosidase - genetics</subject><subject>Biological and medical sciences</subject><subject>DNA Restriction Enzymes</subject><subject>DNA Transposable Elements</subject><subject>Endonucleases</subject><subject>Escherichia coli - enzymology</subject><subject>Escherichia coli - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Galactosidases - genetics</subject><subject>Genes</subject><subject>Genes, Bacterial</subject><subject>Genes, Regulator</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Nucleic Acid Conformation</subject><subject>Plasmids</subject><subject>Single-Strand Specific DNA and RNA Endonucleases</subject><subject>Terminator Regions, Genetic</subject><subject>Transcription, Genetic</subject><subject>Transcription. Transcription factor. Splicing. Rna processing</subject><issn>0305-1048</issn><issn>1362-4962</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1vVCEUxYnR1LG6dWfCwrh7Uy7w4LFw0TbWMZnoZkyMG8Ljo6JvYIQ31f730nYy0ZUrDvf8zs0JIPQSyBKIYmfJlDNgS6BLTql8hBbABO24EvQxWhBG-g4IH56iZ7V-JwQ49PwEnVA1KKnIAs3neIwuFm_nmJOZpltsmrzxuMbrdschFzz7so3J3BE4BzwXk6otcXc_iBVP2ZrZOzz6-Zf3qfFtrUkO5xLWuDH3iV2uTW4SkOfoSTBT9S8O5yn6fPVuc7nq1p_ef7g8X3eR9cPcGUecG4UExiB4x7ykwgnrHIyjU7J3TRoqAzAQpkG873kAGbizTAkxsFP09mHvbj9uvbM-tSKT3pW4NeVWZxP1v06K3_R1vtGMghx4y7855Ev-ufd11ttYrZ8mk3zeVy0FDAMF-l8QFBM9sLuNr_5udKxy-I_mvz74plozhfZuNtYjpugAPZEN6x6wWGf_-2ib8kMLyWSvV1--aoCPV6sLSfSG_QELTauv</recordid><startdate>19850625</startdate><enddate>19850625</enddate><creator>Schollmeier, Klaus</creator><creator>Gärtner, Dagmar</creator><creator>Hillen, Wolfgang</creator><general>Oxford University Press</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7TM</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19850625</creationdate><title>A bidirectionally active signal for termination of transcription is located between tetA and orfL on transposon Tn10</title><author>Schollmeier, Klaus ; Gärtner, Dagmar ; Hillen, Wolfgang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-i358t-ad0ddb671331fed3e726d6cdd1bbd975dcdda27f1316a1334554f17f4dc396683</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1985</creationdate><topic>Bacterial Proteins - genetics</topic><topic>Base Sequence</topic><topic>beta-Galactosidase - genetics</topic><topic>Biological and medical sciences</topic><topic>DNA Restriction Enzymes</topic><topic>DNA Transposable Elements</topic><topic>Endonucleases</topic><topic>Escherichia coli - enzymology</topic><topic>Escherichia coli - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Galactosidases - genetics</topic><topic>Genes</topic><topic>Genes, Bacterial</topic><topic>Genes, Regulator</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Nucleic Acid Conformation</topic><topic>Plasmids</topic><topic>Single-Strand Specific DNA and RNA Endonucleases</topic><topic>Terminator Regions, Genetic</topic><topic>Transcription, Genetic</topic><topic>Transcription. Transcription factor. Splicing. Rna processing</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Schollmeier, Klaus</creatorcontrib><creatorcontrib>Gärtner, Dagmar</creatorcontrib><creatorcontrib>Hillen, Wolfgang</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Nucleic acids research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Schollmeier, Klaus</au><au>Gärtner, Dagmar</au><au>Hillen, Wolfgang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A bidirectionally active signal for termination of transcription is located between tetA and orfL on transposon Tn10</atitle><jtitle>Nucleic acids research</jtitle><addtitle>Nucleic Acids Res</addtitle><date>1985-06-25</date><risdate>1985</risdate><volume>13</volume><issue>12</issue><spage>4227</spage><epage>4237</epage><pages>4227-4237</pages><issn>0305-1048</issn><eissn>1362-4962</eissn><coden>NARHAD</coden><abstract>A terminator of transcription with bidirectional activity has been located between the translation termination codons of the genes tetA and orfL on Tn 10. These genes are transcribed towards each other. Each orientation of the intervening sequence is shown to reduce the expression of the lacZ and galK genes when cloned between the respective structural gene and its promotor. The 3′ends of the respective mRNAs were determined by S1 mapping. The results confirm that the same sequence capable of forming a stem-loop structure with a GC rich stem is the termination signal for both orientations. In the more efficient tetA orientation (99%–96% reduction of expression) this sequence is followed by a run of six thymines. In the less efficient orfL orientation (96%–78% reduction of expression) it is followed by an AT rich sequence with seven thymines out of eleven base pairs</abstract><cop>Oxford</cop><pub>Oxford University Press</pub><pmid>2989790</pmid><doi>10.1093/nar/13.12.4227</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0305-1048 |
| ispartof | Nucleic acids research, 1985-06, Vol.13 (12), p.4227-4237 |
| issn | 0305-1048 1362-4962 |
| language | eng |
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| source | MEDLINE; PubMed Central; Oxford University Press Journals Digital Archive Legacy |
| subjects | Bacterial Proteins - genetics Base Sequence beta-Galactosidase - genetics Biological and medical sciences DNA Restriction Enzymes DNA Transposable Elements Endonucleases Escherichia coli - enzymology Escherichia coli - genetics Fundamental and applied biological sciences. Psychology Galactosidases - genetics Genes Genes, Bacterial Genes, Regulator Molecular and cellular biology Molecular genetics Nucleic Acid Conformation Plasmids Single-Strand Specific DNA and RNA Endonucleases Terminator Regions, Genetic Transcription, Genetic Transcription. Transcription factor. Splicing. Rna processing |
| title | A bidirectionally active signal for termination of transcription is located between tetA and orfL on transposon Tn10 |
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