Gel electrophoresis of partially denatured DNA. Retardation effect: its analysis and application

The hypothesis about the role of partial denaturatlon in DNA retardation during its electrophoreeis in denaturing gel/1,/2 was tested. We used partially melted DNA molecules in which the size of the melted regions and their location were known. They were obtained through glyoxal treatment of the mel...

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Veröffentlicht in:	Nucleic acids research 1982-08, Vol.10 (15), p.4813-4826
Hauptverfasser:	Lyamichev, V.I., Panyutin, I.G., Lyubchenko, Yu.L.
Format:	Artikel
Sprache:	eng
Schlagworte:	DNA - isolation & purification DNA, Bacterial - isolation & purification DNA, Viral - isolation & purification Electrophoresis, Polyacrylamide Gel - methods Indicators and Reagents Molecular Weight Nucleic Acid Denaturation Plasmids
Online-Zugang:	Volltext
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