Gel electrophoresis of partially denatured DNA. Retardation effect: its analysis and application
The hypothesis about the role of partial denaturatlon in DNA retardation during its electrophoreeis in denaturing gel/1,/2 was tested. We used partially melted DNA molecules in which the size of the melted regions and their location were known. They were obtained through glyoxal treatment of the mel...
Gespeichert in:
| Veröffentlicht in: | Nucleic acids research 1982-08, Vol.10 (15), p.4813-4826 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 4826 |
|---|---|
| container_issue | 15 |
| container_start_page | 4813 |
| container_title | Nucleic acids research |
| container_volume | 10 |
| creator | Lyamichev, V.I. Panyutin, I.G. Lyubchenko, Yu.L. |
| description | The hypothesis about the role of partial denaturatlon in DNA retardation during its electrophoreeis in denaturing gel/1,/2 was tested. We used partially melted DNA molecules in which the size of the melted regions and their location were known. They were obtained through glyoxal treatment of the melted regions by a prooedure allowing the denatured state to be fixed at any point within the melting range. The approach and the availability of the melting maps of DNAs made it possible to investigate DNA molecules differing in length and in the size of the melted regions. The presence of a denatured region at the end of the molecule or inside of it was shown to decrease its electrophoretio mobility, the effect depending on the size of the melted region and on the DNA length. On the basis of the experimental results an explanation is proposed for the cause of retardation in the case of partially denatured DNA. |
| doi_str_mv | 10.1093/nar/10.15.4813 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_321131</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>19351840</sourcerecordid><originalsourceid>FETCH-LOGICAL-c484t-187f61a68241b1fb8648619fac8a784dd1ea509ad93213b14a1ff353ee01de7f3</originalsourceid><addsrcrecordid>eNqFkUFvEzEQRlcIVErhyg3JJ26betb2ro3EoUohRUSAECDUi5nsjqnB2V1sB5F_z6aJIjj15JG-90YefUXxFPgMuBHnPcbz3axmUoO4V5yCqKtSmrq6X5xywVUJXOqHxaOUfnAOEpQ8KU4aEMIYc1p8W1BgFKjNcRhvhkjJJzY4NmLMHkPYso56zJtIHbt8dzFjHylj7DD7oWfk3CS-YD4nhj2G7U7GvmM4jsG3t9Dj4oHDkOjJ4T0rPr9-9Wl-VS7fL97ML5ZlK7XMJejG1YC1riSswK10LXUNxmGrsdGy64BQcYOdERWIFUgE54QSRBw6apw4K17u946b1Zq6lvocMdgx-jXGrR3Q2_-T3t_Y78NvO-0DAZP__ODH4deGUrZrn1oKAXsaNsk2sqpBVfJOEIxQoCW_G1RS60Y1Ezjbg20cUorkjr8Gbncl26nk21nZXcmT8OzfW4_4odUpL_e5T5n-HGOMP23diEbZq6_XVi8-XC_n5q39Iv4Ct_Oz5g</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>15488757</pqid></control><display><type>article</type><title>Gel electrophoresis of partially denatured DNA. Retardation effect: its analysis and application</title><source>MEDLINE</source><source>Oxford University Press Journals Digital Archive Legacy</source><source>PubMed Central</source><creator>Lyamichev, V.I. ; Panyutin, I.G. ; Lyubchenko, Yu.L.</creator><creatorcontrib>Lyamichev, V.I. ; Panyutin, I.G. ; Lyubchenko, Yu.L.</creatorcontrib><description>The hypothesis about the role of partial denaturatlon in DNA retardation during its electrophoreeis in denaturing gel/1,/2 was tested. We used partially melted DNA molecules in which the size of the melted regions and their location were known. They were obtained through glyoxal treatment of the melted regions by a prooedure allowing the denatured state to be fixed at any point within the melting range. The approach and the availability of the melting maps of DNAs made it possible to investigate DNA molecules differing in length and in the size of the melted regions. The presence of a denatured region at the end of the molecule or inside of it was shown to decrease its electrophoretio mobility, the effect depending on the size of the melted region and on the DNA length. On the basis of the experimental results an explanation is proposed for the cause of retardation in the case of partially denatured DNA.</description><identifier>ISSN: 0305-1048</identifier><identifier>EISSN: 1362-4962</identifier><identifier>DOI: 10.1093/nar/10.15.4813</identifier><identifier>PMID: 7133999</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>DNA - isolation & purification ; DNA, Bacterial - isolation & purification ; DNA, Viral - isolation & purification ; Electrophoresis, Polyacrylamide Gel - methods ; Indicators and Reagents ; Molecular Weight ; Nucleic Acid Denaturation ; Plasmids</subject><ispartof>Nucleic acids research, 1982-08, Vol.10 (15), p.4813-4826</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c484t-187f61a68241b1fb8648619fac8a784dd1ea509ad93213b14a1ff353ee01de7f3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC321131/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC321131/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7133999$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lyamichev, V.I.</creatorcontrib><creatorcontrib>Panyutin, I.G.</creatorcontrib><creatorcontrib>Lyubchenko, Yu.L.</creatorcontrib><title>Gel electrophoresis of partially denatured DNA. Retardation effect: its analysis and application</title><title>Nucleic acids research</title><addtitle>Nucleic Acids Res</addtitle><description>The hypothesis about the role of partial denaturatlon in DNA retardation during its electrophoreeis in denaturing gel/1,/2 was tested. We used partially melted DNA molecules in which the size of the melted regions and their location were known. They were obtained through glyoxal treatment of the melted regions by a prooedure allowing the denatured state to be fixed at any point within the melting range. The approach and the availability of the melting maps of DNAs made it possible to investigate DNA molecules differing in length and in the size of the melted regions. The presence of a denatured region at the end of the molecule or inside of it was shown to decrease its electrophoretio mobility, the effect depending on the size of the melted region and on the DNA length. On the basis of the experimental results an explanation is proposed for the cause of retardation in the case of partially denatured DNA.</description><subject>DNA - isolation & purification</subject><subject>DNA, Bacterial - isolation & purification</subject><subject>DNA, Viral - isolation & purification</subject><subject>Electrophoresis, Polyacrylamide Gel - methods</subject><subject>Indicators and Reagents</subject><subject>Molecular Weight</subject><subject>Nucleic Acid Denaturation</subject><subject>Plasmids</subject><issn>0305-1048</issn><issn>1362-4962</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1982</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUFvEzEQRlcIVErhyg3JJ26betb2ro3EoUohRUSAECDUi5nsjqnB2V1sB5F_z6aJIjj15JG-90YefUXxFPgMuBHnPcbz3axmUoO4V5yCqKtSmrq6X5xywVUJXOqHxaOUfnAOEpQ8KU4aEMIYc1p8W1BgFKjNcRhvhkjJJzY4NmLMHkPYso56zJtIHbt8dzFjHylj7DD7oWfk3CS-YD4nhj2G7U7GvmM4jsG3t9Dj4oHDkOjJ4T0rPr9-9Wl-VS7fL97ML5ZlK7XMJejG1YC1riSswK10LXUNxmGrsdGy64BQcYOdERWIFUgE54QSRBw6apw4K17u946b1Zq6lvocMdgx-jXGrR3Q2_-T3t_Y78NvO-0DAZP__ODH4deGUrZrn1oKAXsaNsk2sqpBVfJOEIxQoCW_G1RS60Y1Ezjbg20cUorkjr8Gbncl26nk21nZXcmT8OzfW4_4odUpL_e5T5n-HGOMP23diEbZq6_XVi8-XC_n5q39Iv4Ct_Oz5g</recordid><startdate>19820811</startdate><enddate>19820811</enddate><creator>Lyamichev, V.I.</creator><creator>Panyutin, I.G.</creator><creator>Lyubchenko, Yu.L.</creator><general>Oxford University Press</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19820811</creationdate><title>Gel electrophoresis of partially denatured DNA. Retardation effect: its analysis and application</title><author>Lyamichev, V.I. ; Panyutin, I.G. ; Lyubchenko, Yu.L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c484t-187f61a68241b1fb8648619fac8a784dd1ea509ad93213b14a1ff353ee01de7f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1982</creationdate><topic>DNA - isolation & purification</topic><topic>DNA, Bacterial - isolation & purification</topic><topic>DNA, Viral - isolation & purification</topic><topic>Electrophoresis, Polyacrylamide Gel - methods</topic><topic>Indicators and Reagents</topic><topic>Molecular Weight</topic><topic>Nucleic Acid Denaturation</topic><topic>Plasmids</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lyamichev, V.I.</creatorcontrib><creatorcontrib>Panyutin, I.G.</creatorcontrib><creatorcontrib>Lyubchenko, Yu.L.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Nucleic acids research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lyamichev, V.I.</au><au>Panyutin, I.G.</au><au>Lyubchenko, Yu.L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Gel electrophoresis of partially denatured DNA. Retardation effect: its analysis and application</atitle><jtitle>Nucleic acids research</jtitle><addtitle>Nucleic Acids Res</addtitle><date>1982-08-11</date><risdate>1982</risdate><volume>10</volume><issue>15</issue><spage>4813</spage><epage>4826</epage><pages>4813-4826</pages><issn>0305-1048</issn><eissn>1362-4962</eissn><abstract>The hypothesis about the role of partial denaturatlon in DNA retardation during its electrophoreeis in denaturing gel/1,/2 was tested. We used partially melted DNA molecules in which the size of the melted regions and their location were known. They were obtained through glyoxal treatment of the melted regions by a prooedure allowing the denatured state to be fixed at any point within the melting range. The approach and the availability of the melting maps of DNAs made it possible to investigate DNA molecules differing in length and in the size of the melted regions. The presence of a denatured region at the end of the molecule or inside of it was shown to decrease its electrophoretio mobility, the effect depending on the size of the melted region and on the DNA length. On the basis of the experimental results an explanation is proposed for the cause of retardation in the case of partially denatured DNA.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>7133999</pmid><doi>10.1093/nar/10.15.4813</doi><tpages>14</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0305-1048 |
| ispartof | Nucleic acids research, 1982-08, Vol.10 (15), p.4813-4826 |
| issn | 0305-1048 1362-4962 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_321131 |
| source | MEDLINE; Oxford University Press Journals Digital Archive Legacy; PubMed Central |
| subjects | DNA - isolation & purification DNA, Bacterial - isolation & purification DNA, Viral - isolation & purification Electrophoresis, Polyacrylamide Gel - methods Indicators and Reagents Molecular Weight Nucleic Acid Denaturation Plasmids |
| title | Gel electrophoresis of partially denatured DNA. Retardation effect: its analysis and application |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-02T21%3A05%3A43IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Gel%20electrophoresis%20of%20partially%20denatured%20DNA.%20Retardation%20effect:%20its%20analysis%20and%20application&rft.jtitle=Nucleic%20acids%20research&rft.au=Lyamichev,%20V.I.&rft.date=1982-08-11&rft.volume=10&rft.issue=15&rft.spage=4813&rft.epage=4826&rft.pages=4813-4826&rft.issn=0305-1048&rft.eissn=1362-4962&rft_id=info:doi/10.1093/nar/10.15.4813&rft_dat=%3Cproquest_pubme%3E19351840%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=15488757&rft_id=info:pmid/7133999&rfr_iscdi=true |