Epsilon globin gene transcripts originating upstream of the mRNA cap site in K562 cells and normal human embryos
RNA transcribed from the human epsilon globin gene was studied in K562 cells and human embryos of 5–10 weeks gestation. Using both primer extension and S1 analysis RNA molecules were found which extend 53±1 bp, 55±1 bp and 270±1 bp upstream from the first coding base and are colinear with the gene....
Gespeichert in:
| Veröffentlicht in: | Nucleic acids research 1982-09, Vol.10 (17), p.5133-5147 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 5147 |
|---|---|
| container_issue | 17 |
| container_start_page | 5133 |
| container_title | Nucleic acids research |
| container_volume | 10 |
| creator | Alan, Maggi Grindlay, G. Joan Stefani, Lorraine Paul, John |
| description | RNA transcribed from the human epsilon globin gene was studied in K562 cells and human embryos of 5–10 weeks gestation. Using both primer extension and S1 analysis RNA molecules were found which extend 53±1 bp, 55±1 bp and 270±1 bp upstream from the first coding base and are colinear with the gene. The first pair of molecules represent transcripts initiating at the canonical cap site but the other species represents a transcript over 200 nucleotides longer which initiates upstream of the CAAT and ATAA boxes. |
| doi_str_mv | 10.1093/nar/10.17.5133 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_320860</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>15503432</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3993-eb8104f7d85477fcfeaaea3b8bdd97c9841038ea80e64fdea39f22d9d7f5ca643</originalsourceid><addsrcrecordid>eNqFUU1v1DAUtBBVWQpXbkg-ccvWH3FsHzhUq8IiKipVfKy4WE7ysmtI7GAniP77etnVCk49vZFm5mlGg9ArSpaUaH7pbbzcY7kUlPMnaEF5xYpSV-wpWhBOREFJqZ6h5yn9IISWVJTn6LximilOF2i8HpPrg8fbPtQuH_CAp2h9aqIbp4RDdFvn7eT8Fs9jmiLYAYcOTzvAw92nK9zYESc3Ac7uj6JiuIG-T9j6FvsQB9vj3TxYj2Go431IL9BZZ_sEL4_3An15d_15tS5ubt9_WF3dFA3XmhdQq5y7k60SpZRd04G1YHmt6rbVstGqpIQrsIpAVXZtpnTHWKtb2YnGViW_QG8Pf8e5HqBtwOdWvRmjG2y8N8E68z_j3c5sw2_DGVEVyf43R38Mv2ZIkxlc2lezHsKcjCJU56SPC6muqGZCPy4UgvCSsyxcHoRNDClF6E6pKTH70U0e_S-WZj96Nrz-t-tJflw588WBd2mCPyfaxp-mklwKs958N3yt2Orb143Z8AeTMbnS</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>15503432</pqid></control><display><type>article</type><title>Epsilon globin gene transcripts originating upstream of the mRNA cap site in K562 cells and normal human embryos</title><source>MEDLINE</source><source>Oxford University Press Journals Digital Archive Legacy</source><source>PubMed Central</source><creator>Alan, Maggi ; Grindlay, G. Joan ; Stefani, Lorraine ; Paul, John</creator><creatorcontrib>Alan, Maggi ; Grindlay, G. Joan ; Stefani, Lorraine ; Paul, John</creatorcontrib><description>RNA transcribed from the human epsilon globin gene was studied in K562 cells and human embryos of 5–10 weeks gestation. Using both primer extension and S1 analysis RNA molecules were found which extend 53±1 bp, 55±1 bp and 270±1 bp upstream from the first coding base and are colinear with the gene. The first pair of molecules represent transcripts initiating at the canonical cap site but the other species represents a transcript over 200 nucleotides longer which initiates upstream of the CAAT and ATAA boxes.</description><identifier>ISSN: 0305-1048</identifier><identifier>EISSN: 1362-4962</identifier><identifier>DOI: 10.1093/nar/10.17.5133</identifier><identifier>PMID: 6292831</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>Base Composition ; Cell Line ; DNA Restriction Enzymes ; Embryo, Mammalian ; Female ; Fetus ; Genes ; Globins - genetics ; Hemoglobins - genetics ; Humans ; Leukemia, Lymphoid ; Nucleic Acid Hybridization ; Pregnancy ; RNA Caps - genetics ; RNA, Messenger - genetics ; Transcription, Genetic</subject><ispartof>Nucleic acids research, 1982-09, Vol.10 (17), p.5133-5147</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3993-eb8104f7d85477fcfeaaea3b8bdd97c9841038ea80e64fdea39f22d9d7f5ca643</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC320860/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC320860/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,725,778,782,883,27907,27908,53774,53776</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6292831$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Alan, Maggi</creatorcontrib><creatorcontrib>Grindlay, G. Joan</creatorcontrib><creatorcontrib>Stefani, Lorraine</creatorcontrib><creatorcontrib>Paul, John</creatorcontrib><title>Epsilon globin gene transcripts originating upstream of the mRNA cap site in K562 cells and normal human embryos</title><title>Nucleic acids research</title><addtitle>Nucleic Acids Res</addtitle><description>RNA transcribed from the human epsilon globin gene was studied in K562 cells and human embryos of 5–10 weeks gestation. Using both primer extension and S1 analysis RNA molecules were found which extend 53±1 bp, 55±1 bp and 270±1 bp upstream from the first coding base and are colinear with the gene. The first pair of molecules represent transcripts initiating at the canonical cap site but the other species represents a transcript over 200 nucleotides longer which initiates upstream of the CAAT and ATAA boxes.</description><subject>Base Composition</subject><subject>Cell Line</subject><subject>DNA Restriction Enzymes</subject><subject>Embryo, Mammalian</subject><subject>Female</subject><subject>Fetus</subject><subject>Genes</subject><subject>Globins - genetics</subject><subject>Hemoglobins - genetics</subject><subject>Humans</subject><subject>Leukemia, Lymphoid</subject><subject>Nucleic Acid Hybridization</subject><subject>Pregnancy</subject><subject>RNA Caps - genetics</subject><subject>RNA, Messenger - genetics</subject><subject>Transcription, Genetic</subject><issn>0305-1048</issn><issn>1362-4962</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1982</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUU1v1DAUtBBVWQpXbkg-ccvWH3FsHzhUq8IiKipVfKy4WE7ysmtI7GAniP77etnVCk49vZFm5mlGg9ArSpaUaH7pbbzcY7kUlPMnaEF5xYpSV-wpWhBOREFJqZ6h5yn9IISWVJTn6LximilOF2i8HpPrg8fbPtQuH_CAp2h9aqIbp4RDdFvn7eT8Fs9jmiLYAYcOTzvAw92nK9zYESc3Ac7uj6JiuIG-T9j6FvsQB9vj3TxYj2Go431IL9BZZ_sEL4_3An15d_15tS5ubt9_WF3dFA3XmhdQq5y7k60SpZRd04G1YHmt6rbVstGqpIQrsIpAVXZtpnTHWKtb2YnGViW_QG8Pf8e5HqBtwOdWvRmjG2y8N8E68z_j3c5sw2_DGVEVyf43R38Mv2ZIkxlc2lezHsKcjCJU56SPC6muqGZCPy4UgvCSsyxcHoRNDClF6E6pKTH70U0e_S-WZj96Nrz-t-tJflw588WBd2mCPyfaxp-mklwKs958N3yt2Orb143Z8AeTMbnS</recordid><startdate>19820911</startdate><enddate>19820911</enddate><creator>Alan, Maggi</creator><creator>Grindlay, G. Joan</creator><creator>Stefani, Lorraine</creator><creator>Paul, John</creator><general>Oxford University Press</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19820911</creationdate><title>Epsilon globin gene transcripts originating upstream of the mRNA cap site in K562 cells and normal human embryos</title><author>Alan, Maggi ; Grindlay, G. Joan ; Stefani, Lorraine ; Paul, John</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3993-eb8104f7d85477fcfeaaea3b8bdd97c9841038ea80e64fdea39f22d9d7f5ca643</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1982</creationdate><topic>Base Composition</topic><topic>Cell Line</topic><topic>DNA Restriction Enzymes</topic><topic>Embryo, Mammalian</topic><topic>Female</topic><topic>Fetus</topic><topic>Genes</topic><topic>Globins - genetics</topic><topic>Hemoglobins - genetics</topic><topic>Humans</topic><topic>Leukemia, Lymphoid</topic><topic>Nucleic Acid Hybridization</topic><topic>Pregnancy</topic><topic>RNA Caps - genetics</topic><topic>RNA, Messenger - genetics</topic><topic>Transcription, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Alan, Maggi</creatorcontrib><creatorcontrib>Grindlay, G. Joan</creatorcontrib><creatorcontrib>Stefani, Lorraine</creatorcontrib><creatorcontrib>Paul, John</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Nucleic acids research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Alan, Maggi</au><au>Grindlay, G. Joan</au><au>Stefani, Lorraine</au><au>Paul, John</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Epsilon globin gene transcripts originating upstream of the mRNA cap site in K562 cells and normal human embryos</atitle><jtitle>Nucleic acids research</jtitle><addtitle>Nucleic Acids Res</addtitle><date>1982-09-11</date><risdate>1982</risdate><volume>10</volume><issue>17</issue><spage>5133</spage><epage>5147</epage><pages>5133-5147</pages><issn>0305-1048</issn><eissn>1362-4962</eissn><abstract>RNA transcribed from the human epsilon globin gene was studied in K562 cells and human embryos of 5–10 weeks gestation. Using both primer extension and S1 analysis RNA molecules were found which extend 53±1 bp, 55±1 bp and 270±1 bp upstream from the first coding base and are colinear with the gene. The first pair of molecules represent transcripts initiating at the canonical cap site but the other species represents a transcript over 200 nucleotides longer which initiates upstream of the CAAT and ATAA boxes.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>6292831</pmid><doi>10.1093/nar/10.17.5133</doi><tpages>15</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0305-1048 |
| ispartof | Nucleic acids research, 1982-09, Vol.10 (17), p.5133-5147 |
| issn | 0305-1048 1362-4962 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_320860 |
| source | MEDLINE; Oxford University Press Journals Digital Archive Legacy; PubMed Central |
| subjects | Base Composition Cell Line DNA Restriction Enzymes Embryo, Mammalian Female Fetus Genes Globins - genetics Hemoglobins - genetics Humans Leukemia, Lymphoid Nucleic Acid Hybridization Pregnancy RNA Caps - genetics RNA, Messenger - genetics Transcription, Genetic |
| title | Epsilon globin gene transcripts originating upstream of the mRNA cap site in K562 cells and normal human embryos |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-16T13%3A11%3A29IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Epsilon%20globin%20gene%20transcripts%20originating%20upstream%20of%20the%20mRNA%20cap%20site%20in%20K562%20cells%20and%20normal%20human%20embryos&rft.jtitle=Nucleic%20acids%20research&rft.au=Alan,%20Maggi&rft.date=1982-09-11&rft.volume=10&rft.issue=17&rft.spage=5133&rft.epage=5147&rft.pages=5133-5147&rft.issn=0305-1048&rft.eissn=1362-4962&rft_id=info:doi/10.1093/nar/10.17.5133&rft_dat=%3Cproquest_pubme%3E15503432%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=15503432&rft_id=info:pmid/6292831&rfr_iscdi=true |