Mechanisms of Edible Bird's Nest Extract-Induced Proliferation of Human Adipose-Derived Stem Cells
Although edible bird's nest (EBN) has been shown to potentiate mitogenic responses, scientific evidence of its efficacy is still limited. In addition, human adipose-derived stem cells (hADSCs) are increasingly accepted as a source for stem cell therapy. Therefore, the aim of this study was to i...
Gespeichert in:
| Veröffentlicht in: | Evidence-based complementary and alternative medicine 2012-01, Vol.2012 (2012), p.1-11 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 11 |
|---|---|
| container_issue | 2012 |
| container_start_page | 1 |
| container_title | Evidence-based complementary and alternative medicine |
| container_volume | 2012 |
| creator | Park, Deokhoon Kim, Jang-Hyun Park, Junho Kim, Young-Soo Lee, Jienny Roh, Kyung-Baeg Lee, Jongsung |
| description | Although edible bird's nest (EBN) has been shown to potentiate mitogenic responses, scientific evidence of its efficacy is still limited. In addition, human adipose-derived stem cells (hADSCs) are increasingly accepted as a source for stem cell therapy. Therefore, the aim of this study was to investigate the effects of the EBN extract (EBNE) on the proliferation of hADSCs and its action mechanisms. We found that EBNE strongly promoted the proliferation of hADSCs. In addition, EBNE-induced proliferation was found to be mediated through the production of IL-6 and VEGF, which was induced by activation of AP-1 and NF-κB. Specially, we found that production of IL-6 and VEGF was induced by EBNE. In addition, EBNE-induced production of IL-6 and VEGF was inhibited by PD98059 (a p44/42 MAPK inhibitor), SB203580 (a p38 MAPK inhibitor), and PDTC (a NF-κB inhibitor), but not SP600125 (a JNK inhibitor). Similarly, EBNE-induced proliferation of hADSCs was also attenuated by PD98059, SB203580, and PDTC but not SP600125. Taken together, these findings suggest that the EBNE-induced proliferation of hADSCs primarily occurs through increased expression of IL-6 and VEGF genes, which is mediated by the activation of NF-κB and AP-1 through p44/42 MAPK and p38 MAPK. |
| doi_str_mv | 10.1155/2012/797520 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3206510</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2060811526</sourcerecordid><originalsourceid>FETCH-LOGICAL-c465t-62790c2f73f4fd5ca54206ebc1cf942b0db0f17d63b740c8bafcaa7b32cf69d53</originalsourceid><addsrcrecordid>eNqFkc1rFTEUxUNR-mVXrpUBFwVlbJKZTN5shPb1aQttFargLuTjxpcyk7wmM9X-9-Yx7cO66eoG7i_nnsNB6DXBHwlh7IhiQo94yxnFW2iX8JqUNZ3NXmze_OcO2kvpBmPacs630Q6lhGBW812kLkEvpXepT0WwxcI41UFx4qI5TMUVpKFY_Bmi1EN57s2owRTfYuichSgHF_z6z9nYS18cG7cKCcpTiO4uY9cD9MUcui69Qi-t7BIcPMx99OPz4vv8rLz4-uV8fnxR6rphQ9lQ3mJNLa9sbQ3TktUUN6A00batqcJGYUu4aSrFa6xnSlotJVcV1bZpDav20adJdzWqHowGn413YhVdL-O9CNKJpxvvluJXuBNVvsMIzgKHDwIx3I45u-hd0jmC9BDGJFpCWtrgan3q3X_kTRijz-lE1sKz3AttMvVhonQMKUWwGy8Ei3V1Yl2dmKrL9Nt_7W_Yx64y8H4Cls4b-ds9o_ZmgiEjYOUGbnMERqu_ZRWq6A</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2060811526</pqid></control><display><type>article</type><title>Mechanisms of Edible Bird's Nest Extract-Induced Proliferation of Human Adipose-Derived Stem Cells</title><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central Open Access</source><source>Wiley-Blackwell Open Access Titles</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><creator>Park, Deokhoon ; Kim, Jang-Hyun ; Park, Junho ; Kim, Young-Soo ; Lee, Jienny ; Roh, Kyung-Baeg ; Lee, Jongsung</creator><contributor>Ventura, Carlo ; Carlo Ventura</contributor><creatorcontrib>Park, Deokhoon ; Kim, Jang-Hyun ; Park, Junho ; Kim, Young-Soo ; Lee, Jienny ; Roh, Kyung-Baeg ; Lee, Jongsung ; Ventura, Carlo ; Carlo Ventura</creatorcontrib><description>Although edible bird's nest (EBN) has been shown to potentiate mitogenic responses, scientific evidence of its efficacy is still limited. In addition, human adipose-derived stem cells (hADSCs) are increasingly accepted as a source for stem cell therapy. Therefore, the aim of this study was to investigate the effects of the EBN extract (EBNE) on the proliferation of hADSCs and its action mechanisms. We found that EBNE strongly promoted the proliferation of hADSCs. In addition, EBNE-induced proliferation was found to be mediated through the production of IL-6 and VEGF, which was induced by activation of AP-1 and NF-κB. Specially, we found that production of IL-6 and VEGF was induced by EBNE. In addition, EBNE-induced production of IL-6 and VEGF was inhibited by PD98059 (a p44/42 MAPK inhibitor), SB203580 (a p38 MAPK inhibitor), and PDTC (a NF-κB inhibitor), but not SP600125 (a JNK inhibitor). Similarly, EBNE-induced proliferation of hADSCs was also attenuated by PD98059, SB203580, and PDTC but not SP600125. Taken together, these findings suggest that the EBNE-induced proliferation of hADSCs primarily occurs through increased expression of IL-6 and VEGF genes, which is mediated by the activation of NF-κB and AP-1 through p44/42 MAPK and p38 MAPK.</description><identifier>ISSN: 1741-427X</identifier><identifier>EISSN: 1741-4288</identifier><identifier>DOI: 10.1155/2012/797520</identifier><identifier>PMID: 22110547</identifier><language>eng</language><publisher>Cairo, Egypt: Hindawi Publishing Corporation</publisher><subject>Activation ; Amino acids ; Bone marrow ; Cell growth ; Cell proliferation ; Cytokines ; Enzymes ; Fibroblasts ; Hypoxia ; Interleukin 6 ; Kinases ; MAP kinase ; NF-κB protein ; Penicillin ; Physiology ; Proteins ; Signal transduction ; Stem cells ; Transcription factors ; Vascular endothelial growth factor</subject><ispartof>Evidence-based complementary and alternative medicine, 2012-01, Vol.2012 (2012), p.1-11</ispartof><rights>Copyright © 2012 Kyung-Baeg Roh et al.</rights><rights>Copyright © 2012 Kyung-Baeg Roh et al. This is an open access article distributed under the Creative Commons Attribution License (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. https://creativecommons.org/licenses/by/4.0</rights><rights>Copyright © 2012 Kyung-Baeg Roh et al. 2012</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c465t-62790c2f73f4fd5ca54206ebc1cf942b0db0f17d63b740c8bafcaa7b32cf69d53</citedby><cites>FETCH-LOGICAL-c465t-62790c2f73f4fd5ca54206ebc1cf942b0db0f17d63b740c8bafcaa7b32cf69d53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3206510/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3206510/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,724,777,781,882,27905,27906,53772,53774</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22110547$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Ventura, Carlo</contributor><contributor>Carlo Ventura</contributor><creatorcontrib>Park, Deokhoon</creatorcontrib><creatorcontrib>Kim, Jang-Hyun</creatorcontrib><creatorcontrib>Park, Junho</creatorcontrib><creatorcontrib>Kim, Young-Soo</creatorcontrib><creatorcontrib>Lee, Jienny</creatorcontrib><creatorcontrib>Roh, Kyung-Baeg</creatorcontrib><creatorcontrib>Lee, Jongsung</creatorcontrib><title>Mechanisms of Edible Bird's Nest Extract-Induced Proliferation of Human Adipose-Derived Stem Cells</title><title>Evidence-based complementary and alternative medicine</title><addtitle>Evid Based Complement Alternat Med</addtitle><description>Although edible bird's nest (EBN) has been shown to potentiate mitogenic responses, scientific evidence of its efficacy is still limited. In addition, human adipose-derived stem cells (hADSCs) are increasingly accepted as a source for stem cell therapy. Therefore, the aim of this study was to investigate the effects of the EBN extract (EBNE) on the proliferation of hADSCs and its action mechanisms. We found that EBNE strongly promoted the proliferation of hADSCs. In addition, EBNE-induced proliferation was found to be mediated through the production of IL-6 and VEGF, which was induced by activation of AP-1 and NF-κB. Specially, we found that production of IL-6 and VEGF was induced by EBNE. In addition, EBNE-induced production of IL-6 and VEGF was inhibited by PD98059 (a p44/42 MAPK inhibitor), SB203580 (a p38 MAPK inhibitor), and PDTC (a NF-κB inhibitor), but not SP600125 (a JNK inhibitor). Similarly, EBNE-induced proliferation of hADSCs was also attenuated by PD98059, SB203580, and PDTC but not SP600125. Taken together, these findings suggest that the EBNE-induced proliferation of hADSCs primarily occurs through increased expression of IL-6 and VEGF genes, which is mediated by the activation of NF-κB and AP-1 through p44/42 MAPK and p38 MAPK.</description><subject>Activation</subject><subject>Amino acids</subject><subject>Bone marrow</subject><subject>Cell growth</subject><subject>Cell proliferation</subject><subject>Cytokines</subject><subject>Enzymes</subject><subject>Fibroblasts</subject><subject>Hypoxia</subject><subject>Interleukin 6</subject><subject>Kinases</subject><subject>MAP kinase</subject><subject>NF-κB protein</subject><subject>Penicillin</subject><subject>Physiology</subject><subject>Proteins</subject><subject>Signal transduction</subject><subject>Stem cells</subject><subject>Transcription factors</subject><subject>Vascular endothelial growth factor</subject><issn>1741-427X</issn><issn>1741-4288</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>RHX</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqFkc1rFTEUxUNR-mVXrpUBFwVlbJKZTN5shPb1aQttFargLuTjxpcyk7wmM9X-9-Yx7cO66eoG7i_nnsNB6DXBHwlh7IhiQo94yxnFW2iX8JqUNZ3NXmze_OcO2kvpBmPacs630Q6lhGBW812kLkEvpXepT0WwxcI41UFx4qI5TMUVpKFY_Bmi1EN57s2owRTfYuichSgHF_z6z9nYS18cG7cKCcpTiO4uY9cD9MUcui69Qi-t7BIcPMx99OPz4vv8rLz4-uV8fnxR6rphQ9lQ3mJNLa9sbQ3TktUUN6A00batqcJGYUu4aSrFa6xnSlotJVcV1bZpDav20adJdzWqHowGn413YhVdL-O9CNKJpxvvluJXuBNVvsMIzgKHDwIx3I45u-hd0jmC9BDGJFpCWtrgan3q3X_kTRijz-lE1sKz3AttMvVhonQMKUWwGy8Ei3V1Yl2dmKrL9Nt_7W_Yx64y8H4Cls4b-ds9o_ZmgiEjYOUGbnMERqu_ZRWq6A</recordid><startdate>20120101</startdate><enddate>20120101</enddate><creator>Park, Deokhoon</creator><creator>Kim, Jang-Hyun</creator><creator>Park, Junho</creator><creator>Kim, Young-Soo</creator><creator>Lee, Jienny</creator><creator>Roh, Kyung-Baeg</creator><creator>Lee, Jongsung</creator><general>Hindawi Publishing Corporation</general><general>Hindawi Limited</general><scope>ADJCN</scope><scope>AHFXO</scope><scope>RHU</scope><scope>RHW</scope><scope>RHX</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7T5</scope><scope>7TO</scope><scope>7X7</scope><scope>7XB</scope><scope>88G</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>K9.</scope><scope>KB0</scope><scope>M0S</scope><scope>M2M</scope><scope>M2O</scope><scope>MBDVC</scope><scope>NAPCQ</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PSYQQ</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20120101</creationdate><title>Mechanisms of Edible Bird's Nest Extract-Induced Proliferation of Human Adipose-Derived Stem Cells</title><author>Park, Deokhoon ; Kim, Jang-Hyun ; Park, Junho ; Kim, Young-Soo ; Lee, Jienny ; Roh, Kyung-Baeg ; Lee, Jongsung</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c465t-62790c2f73f4fd5ca54206ebc1cf942b0db0f17d63b740c8bafcaa7b32cf69d53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Activation</topic><topic>Amino acids</topic><topic>Bone marrow</topic><topic>Cell growth</topic><topic>Cell proliferation</topic><topic>Cytokines</topic><topic>Enzymes</topic><topic>Fibroblasts</topic><topic>Hypoxia</topic><topic>Interleukin 6</topic><topic>Kinases</topic><topic>MAP kinase</topic><topic>NF-κB protein</topic><topic>Penicillin</topic><topic>Physiology</topic><topic>Proteins</topic><topic>Signal transduction</topic><topic>Stem cells</topic><topic>Transcription factors</topic><topic>Vascular endothelial growth factor</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Park, Deokhoon</creatorcontrib><creatorcontrib>Kim, Jang-Hyun</creatorcontrib><creatorcontrib>Park, Junho</creatorcontrib><creatorcontrib>Kim, Young-Soo</creatorcontrib><creatorcontrib>Lee, Jienny</creatorcontrib><creatorcontrib>Roh, Kyung-Baeg</creatorcontrib><creatorcontrib>Lee, Jongsung</creatorcontrib><collection>الدوريات العلمية والإحصائية - e-Marefa Academic and Statistical Periodicals</collection><collection>معرفة - المحتوى العربي الأكاديمي المتكامل - e-Marefa Academic Complete</collection><collection>Hindawi Publishing Complete</collection><collection>Hindawi Publishing Subscription Journals</collection><collection>Hindawi Publishing Open Access</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Immunology Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Psychology Database (Alumni)</collection><collection>ProQuest Pharma Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>ProQuest Psychology</collection><collection>Research Library</collection><collection>Research Library (Corporate)</collection><collection>Nursing & Allied Health Premium</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest One Psychology</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Evidence-based complementary and alternative medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Park, Deokhoon</au><au>Kim, Jang-Hyun</au><au>Park, Junho</au><au>Kim, Young-Soo</au><au>Lee, Jienny</au><au>Roh, Kyung-Baeg</au><au>Lee, Jongsung</au><au>Ventura, Carlo</au><au>Carlo Ventura</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mechanisms of Edible Bird's Nest Extract-Induced Proliferation of Human Adipose-Derived Stem Cells</atitle><jtitle>Evidence-based complementary and alternative medicine</jtitle><addtitle>Evid Based Complement Alternat Med</addtitle><date>2012-01-01</date><risdate>2012</risdate><volume>2012</volume><issue>2012</issue><spage>1</spage><epage>11</epage><pages>1-11</pages><issn>1741-427X</issn><eissn>1741-4288</eissn><abstract>Although edible bird's nest (EBN) has been shown to potentiate mitogenic responses, scientific evidence of its efficacy is still limited. In addition, human adipose-derived stem cells (hADSCs) are increasingly accepted as a source for stem cell therapy. Therefore, the aim of this study was to investigate the effects of the EBN extract (EBNE) on the proliferation of hADSCs and its action mechanisms. We found that EBNE strongly promoted the proliferation of hADSCs. In addition, EBNE-induced proliferation was found to be mediated through the production of IL-6 and VEGF, which was induced by activation of AP-1 and NF-κB. Specially, we found that production of IL-6 and VEGF was induced by EBNE. In addition, EBNE-induced production of IL-6 and VEGF was inhibited by PD98059 (a p44/42 MAPK inhibitor), SB203580 (a p38 MAPK inhibitor), and PDTC (a NF-κB inhibitor), but not SP600125 (a JNK inhibitor). Similarly, EBNE-induced proliferation of hADSCs was also attenuated by PD98059, SB203580, and PDTC but not SP600125. Taken together, these findings suggest that the EBNE-induced proliferation of hADSCs primarily occurs through increased expression of IL-6 and VEGF genes, which is mediated by the activation of NF-κB and AP-1 through p44/42 MAPK and p38 MAPK.</abstract><cop>Cairo, Egypt</cop><pub>Hindawi Publishing Corporation</pub><pmid>22110547</pmid><doi>10.1155/2012/797520</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1741-427X |
| ispartof | Evidence-based complementary and alternative medicine, 2012-01, Vol.2012 (2012), p.1-11 |
| issn | 1741-427X 1741-4288 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3206510 |
| source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central Open Access; Wiley-Blackwell Open Access Titles; PubMed Central; Alma/SFX Local Collection |
| subjects | Activation Amino acids Bone marrow Cell growth Cell proliferation Cytokines Enzymes Fibroblasts Hypoxia Interleukin 6 Kinases MAP kinase NF-κB protein Penicillin Physiology Proteins Signal transduction Stem cells Transcription factors Vascular endothelial growth factor |
| title | Mechanisms of Edible Bird's Nest Extract-Induced Proliferation of Human Adipose-Derived Stem Cells |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-20T19%3A58%3A52IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Mechanisms%20of%20Edible%20Bird's%20Nest%20Extract-Induced%20Proliferation%20of%20Human%20Adipose-Derived%20Stem%20Cells&rft.jtitle=Evidence-based%20complementary%20and%20alternative%20medicine&rft.au=Park,%20Deokhoon&rft.date=2012-01-01&rft.volume=2012&rft.issue=2012&rft.spage=1&rft.epage=11&rft.pages=1-11&rft.issn=1741-427X&rft.eissn=1741-4288&rft_id=info:doi/10.1155/2012/797520&rft_dat=%3Cproquest_pubme%3E2060811526%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2060811526&rft_id=info:pmid/22110547&rfr_iscdi=true |