Physiological Response to Membrane Protein Overexpression in E. coli

Overexpression represents a principal bottleneck in structural and functional studies of integral membrane proteins (IMPs). Although E. coli remains the leading organism for convenient and economical protein overexpression, many IMPs exhibit toxicity on induction in this host and give low yields of...

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Veröffentlicht in:	Molecular & cellular proteomics 2011-10, Vol.10 (10), p.M111.007930-M111.007930, Article M111.007930
Hauptverfasser:	Gubellini, Francesca, Verdon, Grégory, Karpowich, Nathan K., Luff, Jon D., Boël, Grégory, Gauthier, Nils, Handelman, Samuel K., Ades, Sarah E., Hunt, John F.
Format:	Artikel
Sprache:	eng
Schlagworte:	Biochemistry, Molecular Biology Bioinformatics Biological Physics Cellular Biology Chemical Sciences Computer Science Cristallography Escherichia coli Escherichia coli - genetics Escherichia coli - growth & development Escherichia coli Proteins Escherichia coli Proteins - biosynthesis Escherichia coli Proteins - genetics Gene Expression Regulation, Bacterial Genetic Vectors Life Sciences Membrane Proteins Membrane Proteins - biosynthesis Membrane Proteins - chemistry Membrane Proteins - genetics Physics Protein Array Analysis Protein Array Analysis - methods Protein Folding RNA, Messenger RNA, Messenger - genetics RNA, Messenger - metabolism Structural Biology Transcription Factors Transcription Factors - biosynthesis Transcription Factors - genetics Transcription, Genetic
Online-Zugang:	Volltext
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