The orderly splicing of the first three leaders of the adenovirus-2 major late transcript
A strategy based on the hybridization of labeled nucleoplasmic RNA to a short cloned cDNA probe was devised to study the ligation of the three first leader sequences (Le1, Le2, Le3) of the major late adenovirus-2 transcript. The hybridized RNA was subsequently fractionated by electrophoresis and ide...
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| Veröffentlicht in: | Nucleic acids research 1982-02, Vol.10 (4), p.1215-1229 |
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| container_title | Nucleic acids research |
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| creator | Keohavong, P. Gattoni, R. LeMoullec, J.M. Jacob, M. Stévenin, J. |
| description | A strategy based on the hybridization of labeled nucleoplasmic RNA to a short cloned cDNA probe was devised to study the ligation of the three first leader sequences (Le1, Le2, Le3) of the major late adenovirus-2 transcript. The hybridized RNA was subsequently fractionated by electrophoresis and identified with the aid of restriction fragments of the DNA probe. The ligations were shown to occur stepwise and in an orderly fashion. Le1 and Le2 were first ligated without detectable lag time. The tripartite leader was formed after a lag time of 10–15 min probably due, for a large part, to the stepwise excision of the intervening sequence between Le2 and Le3. The possible processing intermediate Le2-Le3 was not detected. |
| doi_str_mv | 10.1093/nar/10.4.1215 |
| format | Article |
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The hybridized RNA was subsequently fractionated by electrophoresis and identified with the aid of restriction fragments of the DNA probe. The ligations were shown to occur stepwise and in an orderly fashion. Le1 and Le2 were first ligated without detectable lag time. The tripartite leader was formed after a lag time of 10–15 min probably due, for a large part, to the stepwise excision of the intervening sequence between Le2 and Le3. 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The hybridized RNA was subsequently fractionated by electrophoresis and identified with the aid of restriction fragments of the DNA probe. The ligations were shown to occur stepwise and in an orderly fashion. Le1 and Le2 were first ligated without detectable lag time. The tripartite leader was formed after a lag time of 10–15 min probably due, for a large part, to the stepwise excision of the intervening sequence between Le2 and Le3. The possible processing intermediate Le2-Le3 was not detected.</description><subject>Adenoviruses, Human - genetics</subject><subject>Base Composition</subject><subject>Base Sequence</subject><subject>Cloning, Molecular</subject><subject>DNA - metabolism</subject><subject>DNA Restriction Enzymes</subject><subject>HeLa Cells - metabolism</subject><subject>Humans</subject><subject>Nucleic Acid Hybridization</subject><subject>Poly A - genetics</subject><subject>RNA - genetics</subject><subject>RNA, Messenger - genetics</subject><subject>Transcription, Genetic</subject><issn>0305-1048</issn><issn>1362-4962</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1982</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUFPGzEQha2qiKbQY4-V9tTbgsce2_GhhyoqUAkJVQIJuFiOMwumm3Vqb1D593VEmtITJz_7fTOe0WPsI_Aj4FYeDz4fV41HIEC9YROQWrRotXjLJlxy1QLH6Tv2vpQHzgFB4T7b12CUVThhN5f31KS8oNw_NWXVxxCHuyZ1zVjfu5jLWFUmanryFSp_rXoZ0mPM69KKZukfUm56P1IzZj-UkONqPGR7ne8LfdieB-zq5Nvl7Kw9vzj9Pvt63gZptWql4FMQAg0GXJARWkNQqA0FDh6smlu0i6AEwNySgamUBtHPdddZX3fp5AH78tx3tZ4vaRFoqDP0bpXj0ucnl3x0_ztDvHd36dHVn5Xgtf7ztj6nX2sqo1vGEqjv_UBpXZxBjhpQvQqCldpqLl8HVQXV1FawfQZDTqVk6nZTA3ebcF0Nd6PRbcKt_KeXq-7obZr_-sUy0u-d7fNPp400yp1d37rZ7TXC6ckPB_IPpOGvIA</recordid><startdate>19820225</startdate><enddate>19820225</enddate><creator>Keohavong, P.</creator><creator>Gattoni, R.</creator><creator>LeMoullec, J.M.</creator><creator>Jacob, M.</creator><creator>Stévenin, J.</creator><general>Oxford University Press</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19820225</creationdate><title>The orderly splicing of the first three leaders of the adenovirus-2 major late transcript</title><author>Keohavong, P. ; Gattoni, R. ; LeMoullec, J.M. ; Jacob, M. ; Stévenin, J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3965-3208122474c4de72661c5467ec01a195b949dc5211b9e71833744ab6ff9a154f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1982</creationdate><topic>Adenoviruses, Human - genetics</topic><topic>Base Composition</topic><topic>Base Sequence</topic><topic>Cloning, Molecular</topic><topic>DNA - metabolism</topic><topic>DNA Restriction Enzymes</topic><topic>HeLa Cells - metabolism</topic><topic>Humans</topic><topic>Nucleic Acid Hybridization</topic><topic>Poly A - genetics</topic><topic>RNA - genetics</topic><topic>RNA, Messenger - genetics</topic><topic>Transcription, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Keohavong, P.</creatorcontrib><creatorcontrib>Gattoni, R.</creatorcontrib><creatorcontrib>LeMoullec, J.M.</creatorcontrib><creatorcontrib>Jacob, M.</creatorcontrib><creatorcontrib>Stévenin, J.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Nucleic acids research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Keohavong, P.</au><au>Gattoni, R.</au><au>LeMoullec, J.M.</au><au>Jacob, M.</au><au>Stévenin, J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The orderly splicing of the first three leaders of the adenovirus-2 major late transcript</atitle><jtitle>Nucleic acids research</jtitle><addtitle>Nucleic Acids Res</addtitle><date>1982-02-25</date><risdate>1982</risdate><volume>10</volume><issue>4</issue><spage>1215</spage><epage>1229</epage><pages>1215-1229</pages><issn>0305-1048</issn><eissn>1362-4962</eissn><abstract>A strategy based on the hybridization of labeled nucleoplasmic RNA to a short cloned cDNA probe was devised to study the ligation of the three first leader sequences (Le1, Le2, Le3) of the major late adenovirus-2 transcript. The hybridized RNA was subsequently fractionated by electrophoresis and identified with the aid of restriction fragments of the DNA probe. The ligations were shown to occur stepwise and in an orderly fashion. Le1 and Le2 were first ligated without detectable lag time. The tripartite leader was formed after a lag time of 10–15 min probably due, for a large part, to the stepwise excision of the intervening sequence between Le2 and Le3. The possible processing intermediate Le2-Le3 was not detected.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>6175954</pmid><doi>10.1093/nar/10.4.1215</doi><tpages>15</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0305-1048 |
| ispartof | Nucleic acids research, 1982-02, Vol.10 (4), p.1215-1229 |
| issn | 0305-1048 1362-4962 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_320520 |
| source | MEDLINE; PubMed Central; Oxford University Press Journals Digital Archive Legacy |
| subjects | Adenoviruses, Human - genetics Base Composition Base Sequence Cloning, Molecular DNA - metabolism DNA Restriction Enzymes HeLa Cells - metabolism Humans Nucleic Acid Hybridization Poly A - genetics RNA - genetics RNA, Messenger - genetics Transcription, Genetic |
| title | The orderly splicing of the first three leaders of the adenovirus-2 major late transcript |
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