A plasmid vehicle suitable for the molecular cloning and characterization of mammalian promoters

We have constructed a plasmid, pPSG4, that carries only the coding sequences for polyomavirus (Py) small, middle and truncated large T-antigens. A unique Hindi 11 site allows the introduction of foreign promoters directly in front of viral coding sequences. The simian virus 40 (SV40) early and late,...

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Veröffentlicht in:	Nucleic acids research 1984-09, Vol.12 (18), p.7235-7249
Hauptverfasser:	Featherstone, M.S., Naujokas, M.A., Pomerantz, B.J., Hassell, J.A.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antigens, Viral - genetics Base Sequence Cell Line Cell Transformation, Neoplastic Chromosome Deletion cloning vectors Cloning, Molecular DNA Restriction Enzymes DNA, Recombinant - metabolism Genetic Vectors Herpes simplex virus 1 Mammalia Mutation Operon Plasmids Polyomavirus Polyomavirus - genetics promoters Rats Simian virus 40
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