A plasmid vehicle suitable for the molecular cloning and characterization of mammalian promoters
We have constructed a plasmid, pPSG4, that carries only the coding sequences for polyomavirus (Py) small, middle and truncated large T-antigens. A unique Hindi 11 site allows the introduction of foreign promoters directly in front of viral coding sequences. The simian virus 40 (SV40) early and late,...
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| Veröffentlicht in: | Nucleic acids research 1984-09, Vol.12 (18), p.7235-7249 |
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| container_issue | 18 |
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| container_title | Nucleic acids research |
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| creator | Featherstone, M.S. Naujokas, M.A. Pomerantz, B.J. Hassell, J.A. |
| description | We have constructed a plasmid, pPSG4, that carries only the coding sequences for polyomavirus (Py) small, middle and truncated large T-antigens. A unique Hindi 11 site allows the introduction of foreign promoters directly in front of viral coding sequences. The simian virus 40 (SV40) early and late, adenovirus-2 (Ad-2) major late and herpes simplex virus-1 (HSV-1) thymidine kinase (TK) promoters all confer on pPSG4 the ability to transform rat embryonic fibroblasts with high efficiency. Sequential deletion of the 72 bp repeats, the 21 bp repeats and the TATA box from the SV40 early region in pPSG4 produced a 50, then 30 and then a further 5 to 10-fold decrease in transformation efficiency, respectively. Thus pPSG4 is a convenient vector for the cloning and characterization of mammalian promoters. |
| doi_str_mv | 10.1093/nar/12.18.7235 |
| format | Article |
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A unique Hindi 11 site allows the introduction of foreign promoters directly in front of viral coding sequences. The simian virus 40 (SV40) early and late, adenovirus-2 (Ad-2) major late and herpes simplex virus-1 (HSV-1) thymidine kinase (TK) promoters all confer on pPSG4 the ability to transform rat embryonic fibroblasts with high efficiency. Sequential deletion of the 72 bp repeats, the 21 bp repeats and the TATA box from the SV40 early region in pPSG4 produced a 50, then 30 and then a further 5 to 10-fold decrease in transformation efficiency, respectively. 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A unique Hindi 11 site allows the introduction of foreign promoters directly in front of viral coding sequences. The simian virus 40 (SV40) early and late, adenovirus-2 (Ad-2) major late and herpes simplex virus-1 (HSV-1) thymidine kinase (TK) promoters all confer on pPSG4 the ability to transform rat embryonic fibroblasts with high efficiency. Sequential deletion of the 72 bp repeats, the 21 bp repeats and the TATA box from the SV40 early region in pPSG4 produced a 50, then 30 and then a further 5 to 10-fold decrease in transformation efficiency, respectively. Thus pPSG4 is a convenient vector for the cloning and characterization of mammalian promoters.</description><subject>Animals</subject><subject>Antigens, Viral - genetics</subject><subject>Base Sequence</subject><subject>Cell Line</subject><subject>Cell Transformation, Neoplastic</subject><subject>Chromosome Deletion</subject><subject>cloning vectors</subject><subject>Cloning, Molecular</subject><subject>DNA Restriction Enzymes</subject><subject>DNA, Recombinant - metabolism</subject><subject>Genetic Vectors</subject><subject>Herpes simplex virus 1</subject><subject>Mammalia</subject><subject>Mutation</subject><subject>Operon</subject><subject>Plasmids</subject><subject>Polyomavirus</subject><subject>Polyomavirus - genetics</subject><subject>promoters</subject><subject>Rats</subject><subject>Simian virus 40</subject><issn>0305-1048</issn><issn>1362-4962</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1984</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1v1DAQxS0EKkvhyg3JJ27Z-ivO5MChVJQiVSDxIVW9mInjdA2OvdhJBfz1pOxqBaeeZqTfe6OZeYQ852zNWStPIuYTLtYc1o2Q9QOy4lKLSrVaPCQrJlldcabgMXlSyjfGuOK1OiJHmrWcab4iX0_pNmAZfU9v3cbb4GiZ_YTd0gwp02nj6JiCs3PATG1I0ccbirGndoMZ7eSy_42TT5GmgY44jhg8RrrNaUwLLE_JowFDcc_29Zh8OX_z-eyiuvzw9t3Z6WVlFaipGgAHaRvd9D3wWspGWuCsszVjDqB12krseNcP0A-KobK6RdF3rVRLA1DLY_JqN3c7d6PrrYtTxmC22Y-Yf5mE3vxPot-Ym3RrpGDLTxb_y70_px-zK5MZfbEuBIwuzcUAF6ABxL1C3tZ1oxq4XyhbYM3fieud0OZUSnbDYWvOzF3IZgnZcGE4mLuQF8OLf289yPepLrzacV8m9_OAMX83upFNbS6urs3786tPH1_ra9PKPwoytME</recordid><startdate>19840925</startdate><enddate>19840925</enddate><creator>Featherstone, M.S.</creator><creator>Naujokas, M.A.</creator><creator>Pomerantz, B.J.</creator><creator>Hassell, J.A.</creator><general>Oxford University Press</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19840925</creationdate><title>A plasmid vehicle suitable for the molecular cloning and characterization of mammalian promoters</title><author>Featherstone, M.S. ; Naujokas, M.A. ; Pomerantz, B.J. ; Hassell, J.A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c484t-f8af3c767dd8153373c810bc500e889e6c3ab1bdf8df40a4c69a2db93469a8853</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1984</creationdate><topic>Animals</topic><topic>Antigens, Viral - genetics</topic><topic>Base Sequence</topic><topic>Cell Line</topic><topic>Cell Transformation, Neoplastic</topic><topic>Chromosome Deletion</topic><topic>cloning vectors</topic><topic>Cloning, Molecular</topic><topic>DNA Restriction Enzymes</topic><topic>DNA, Recombinant - metabolism</topic><topic>Genetic Vectors</topic><topic>Herpes simplex virus 1</topic><topic>Mammalia</topic><topic>Mutation</topic><topic>Operon</topic><topic>Plasmids</topic><topic>Polyomavirus</topic><topic>Polyomavirus - genetics</topic><topic>promoters</topic><topic>Rats</topic><topic>Simian virus 40</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Featherstone, M.S.</creatorcontrib><creatorcontrib>Naujokas, M.A.</creatorcontrib><creatorcontrib>Pomerantz, B.J.</creatorcontrib><creatorcontrib>Hassell, J.A.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Nucleic acids research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Featherstone, M.S.</au><au>Naujokas, M.A.</au><au>Pomerantz, B.J.</au><au>Hassell, J.A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A plasmid vehicle suitable for the molecular cloning and characterization of mammalian promoters</atitle><jtitle>Nucleic acids research</jtitle><addtitle>Nucleic Acids Res</addtitle><date>1984-09-25</date><risdate>1984</risdate><volume>12</volume><issue>18</issue><spage>7235</spage><epage>7249</epage><pages>7235-7249</pages><issn>0305-1048</issn><eissn>1362-4962</eissn><abstract>We have constructed a plasmid, pPSG4, that carries only the coding sequences for polyomavirus (Py) small, middle and truncated large T-antigens. A unique Hindi 11 site allows the introduction of foreign promoters directly in front of viral coding sequences. The simian virus 40 (SV40) early and late, adenovirus-2 (Ad-2) major late and herpes simplex virus-1 (HSV-1) thymidine kinase (TK) promoters all confer on pPSG4 the ability to transform rat embryonic fibroblasts with high efficiency. Sequential deletion of the 72 bp repeats, the 21 bp repeats and the TATA box from the SV40 early region in pPSG4 produced a 50, then 30 and then a further 5 to 10-fold decrease in transformation efficiency, respectively. Thus pPSG4 is a convenient vector for the cloning and characterization of mammalian promoters.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>6091061</pmid><doi>10.1093/nar/12.18.7235</doi><tpages>15</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Nucleic acids research, 1984-09, Vol.12 (18), p.7235-7249 |
| issn | 0305-1048 1362-4962 |
| language | eng |
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| source | MEDLINE; Oxford University Press Journals Digital Archive Legacy; PubMed Central |
| subjects | Animals Antigens, Viral - genetics Base Sequence Cell Line Cell Transformation, Neoplastic Chromosome Deletion cloning vectors Cloning, Molecular DNA Restriction Enzymes DNA, Recombinant - metabolism Genetic Vectors Herpes simplex virus 1 Mammalia Mutation Operon Plasmids Polyomavirus Polyomavirus - genetics promoters Rats Simian virus 40 |
| title | A plasmid vehicle suitable for the molecular cloning and characterization of mammalian promoters |
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