The expression of ADAM23 and its correlation with promoter methylation in non-small-cell lung carcinoma
Summary ADAM23, a member of a disintegrin and metalloprotease (ADAM) family, has been reported to be expressed in several types of tumours. The exact role of ADAM23 and the possible mechanisms in which it is involved in non‐small‐cell lung carcinoma (NSCLC) remains unclear. Therefore, this study was...
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ADAM23, a member of a disintegrin and metalloprotease (ADAM) family, has been reported to be expressed in several types of tumours. The exact role of ADAM23 and the possible mechanisms in which it is involved in non‐small‐cell lung carcinoma (NSCLC) remains unclear. Therefore, this study was designed to explore the expression of ADAM23 and its correlation with promoter methylation in NSCLC. Immunohistochemistry and RT‐PCR together with Western blotting methods were used to analyse the expression of ADAM23 in 52 cancer tissue samples and eight benign pulmonary lesions as well as four cell lines. The methylated status of ADAM23 gene was determined with methylation‐specific PCR (MSP). The results of immunohistochemistry showed that the expression of ADAM23 protein was lower in NSCLC than that in corresponding normal tissues and benign pulmonary lesions (38.5%vs. 86.5% and 87.5%, P |
| doi_str_mv | 10.1111/j.1365-2613.2011.00766.x |
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ADAM23, a member of a disintegrin and metalloprotease (ADAM) family, has been reported to be expressed in several types of tumours. The exact role of ADAM23 and the possible mechanisms in which it is involved in non‐small‐cell lung carcinoma (NSCLC) remains unclear. Therefore, this study was designed to explore the expression of ADAM23 and its correlation with promoter methylation in NSCLC. Immunohistochemistry and RT‐PCR together with Western blotting methods were used to analyse the expression of ADAM23 in 52 cancer tissue samples and eight benign pulmonary lesions as well as four cell lines. The methylated status of ADAM23 gene was determined with methylation‐specific PCR (MSP). The results of immunohistochemistry showed that the expression of ADAM23 protein was lower in NSCLC than that in corresponding normal tissues and benign pulmonary lesions (38.5%vs. 86.5% and 87.5%, P < 0.05), and decreased as NSCLC progressed. Meanwhile, methylation of ADAM23 gene was observed in 21 of 52 NSCLC tissues (40.4%), much higher than that of adjacent normal tissues (7.6%) and benign pulmonary lesions (0/8). In the cancer tissues of ADAM23‐negative samples, the rate of ADAM23 gene methylation was 50.3% (17/32). ADAM23 expression and its promoter methylation were negatively associated (r = −0.328, P = 0.017). Moreover, weak expression of ADAM23 in methylated cancer cells increased after treatment with 5‐aza‐2′‐deoxycytidine (5‐Aza‐2′‐dC), confirming that methylation was responsible for the gene downregulation. Our results demonstrate that the expression level of ADAM23 is likely to be involved in the progression of NSCLC and its downregulation is probably correlated with promoter methylation. These findings may provide potential diagnostic and prognostic information about NSCLC.</description><identifier>ISSN: 0959-9673</identifier><identifier>EISSN: 1365-2613</identifier><identifier>DOI: 10.1111/j.1365-2613.2011.00766.x</identifier><identifier>PMID: 21429053</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>5-aza-2'-deoxycytidine ; 5-Aza-2′-dC ; ADAM Proteins - genetics ; ADAM Proteins - metabolism ; ADAM23 gene ; Adult ; Aged ; Biomarkers, Tumor - genetics ; Biomarkers, Tumor - metabolism ; cancer cells lines ; Carcinoma, Non-Small-Cell Lung - genetics ; Carcinoma, Non-Small-Cell Lung - metabolism ; Carcinoma, Non-Small-Cell Lung - pathology ; Case-Control Studies ; Cell Line, Tumor ; Gene Expression Regulation, Neoplastic - physiology ; Humans ; Lung - metabolism ; Lung - pathology ; Lung Neoplasms - genetics ; Lung Neoplasms - metabolism ; Lung Neoplasms - pathology ; Methylation ; methylation-specific PCR ; Middle Aged ; non-small-cell lung carcinoma ; Original ; Promoter Regions, Genetic - genetics ; Promoter Regions, Genetic - physiology ; RNA, Messenger - metabolism</subject><ispartof>International journal of experimental pathology, 2011-10, Vol.92 (5), p.333-339</ispartof><rights>2011 The Authors. International Journal of Experimental Pathology © 2011 International Journal of Experimental Pathology</rights><rights>2011 The Authors. International Journal of Experimental Pathology © 2011 International Journal of Experimental Pathology.</rights><rights>International Journal of Experimental Pathology © 2011 International Journal of Experimental Pathology 2011</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5436-c348aa0d3dd58ef858be6f96f73c9eb53fcf61d4460df5436d34be46100cb8a53</citedby><cites>FETCH-LOGICAL-c5436-c348aa0d3dd58ef858be6f96f73c9eb53fcf61d4460df5436d34be46100cb8a53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3193147/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3193147/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,1411,27901,27902,45550,45551,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21429053$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hu, Chunyan</creatorcontrib><creatorcontrib>Lv, Hui</creatorcontrib><creatorcontrib>Pan, Guoqing</creatorcontrib><creatorcontrib>Cao, Huiqiu</creatorcontrib><creatorcontrib>Deng, Zhenghao</creatorcontrib><creatorcontrib>Hu, Chuanyu</creatorcontrib><creatorcontrib>Wen, Jifang</creatorcontrib><creatorcontrib>Zhou, Jianhua</creatorcontrib><title>The expression of ADAM23 and its correlation with promoter methylation in non-small-cell lung carcinoma</title><title>International journal of experimental pathology</title><addtitle>Int J Exp Pathol</addtitle><description>Summary
ADAM23, a member of a disintegrin and metalloprotease (ADAM) family, has been reported to be expressed in several types of tumours. The exact role of ADAM23 and the possible mechanisms in which it is involved in non‐small‐cell lung carcinoma (NSCLC) remains unclear. Therefore, this study was designed to explore the expression of ADAM23 and its correlation with promoter methylation in NSCLC. Immunohistochemistry and RT‐PCR together with Western blotting methods were used to analyse the expression of ADAM23 in 52 cancer tissue samples and eight benign pulmonary lesions as well as four cell lines. The methylated status of ADAM23 gene was determined with methylation‐specific PCR (MSP). The results of immunohistochemistry showed that the expression of ADAM23 protein was lower in NSCLC than that in corresponding normal tissues and benign pulmonary lesions (38.5%vs. 86.5% and 87.5%, P < 0.05), and decreased as NSCLC progressed. Meanwhile, methylation of ADAM23 gene was observed in 21 of 52 NSCLC tissues (40.4%), much higher than that of adjacent normal tissues (7.6%) and benign pulmonary lesions (0/8). In the cancer tissues of ADAM23‐negative samples, the rate of ADAM23 gene methylation was 50.3% (17/32). ADAM23 expression and its promoter methylation were negatively associated (r = −0.328, P = 0.017). Moreover, weak expression of ADAM23 in methylated cancer cells increased after treatment with 5‐aza‐2′‐deoxycytidine (5‐Aza‐2′‐dC), confirming that methylation was responsible for the gene downregulation. Our results demonstrate that the expression level of ADAM23 is likely to be involved in the progression of NSCLC and its downregulation is probably correlated with promoter methylation. These findings may provide potential diagnostic and prognostic information about NSCLC.</description><subject>5-aza-2'-deoxycytidine</subject><subject>5-Aza-2′-dC</subject><subject>ADAM Proteins - genetics</subject><subject>ADAM Proteins - metabolism</subject><subject>ADAM23 gene</subject><subject>Adult</subject><subject>Aged</subject><subject>Biomarkers, Tumor - genetics</subject><subject>Biomarkers, Tumor - metabolism</subject><subject>cancer cells lines</subject><subject>Carcinoma, Non-Small-Cell Lung - genetics</subject><subject>Carcinoma, Non-Small-Cell Lung - metabolism</subject><subject>Carcinoma, Non-Small-Cell Lung - pathology</subject><subject>Case-Control Studies</subject><subject>Cell Line, Tumor</subject><subject>Gene Expression Regulation, Neoplastic - physiology</subject><subject>Humans</subject><subject>Lung - metabolism</subject><subject>Lung - pathology</subject><subject>Lung Neoplasms - genetics</subject><subject>Lung Neoplasms - metabolism</subject><subject>Lung Neoplasms - pathology</subject><subject>Methylation</subject><subject>methylation-specific PCR</subject><subject>Middle Aged</subject><subject>non-small-cell lung carcinoma</subject><subject>Original</subject><subject>Promoter Regions, Genetic - genetics</subject><subject>Promoter Regions, Genetic - physiology</subject><subject>RNA, Messenger - metabolism</subject><issn>0959-9673</issn><issn>1365-2613</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU1v0zAYxy0EYmXwFZBvcElmx7HjSAipGmObNF4ORRwtx3nSuiR2Z6es_fZzaKnggvDFlv4veh7_EMKU5DSdi3VOmeBZISjLC0JpTkglRL57gmYn4SmakZrXWS0qdoZexLgmhLKCVs_RWUHLoiaczdBysQIMu02AGK132Hd4_mH-qWBYuxbbMWLjQ4Bej5P6YMcV3gQ_-BECHmBc7Y-Kddh5l8VB931moO9xv3VLbHQw1vlBv0TPOt1HeHW8z9G3j1eLy5vs7sv17eX8LjO8ZCIzrJRak5a1LZfQSS4bEF0tuoqZGhrOOtMJ2palIG03JVpWNlAKSohppObsHL0_9G62zQCtATcG3atNsIMOe-W1VX8rzq7U0v9UjNaMllUqeHMsCP5-C3FUg43TQtqB30Yla5I-VFKWnG__6aSlkAmG4DJZ5cFqgo8xQHcaiBI1EVVrNYFTEzg1EVW_iKpdir7-c6FT8DfCZHh3MDzYHvb_Xaxur76mR4pnh7iNI-xOcR1-qLRnxdX3z9eKpw5W3yyUYI9Oub-h</recordid><startdate>201110</startdate><enddate>201110</enddate><creator>Hu, Chunyan</creator><creator>Lv, Hui</creator><creator>Pan, Guoqing</creator><creator>Cao, Huiqiu</creator><creator>Deng, Zhenghao</creator><creator>Hu, Chuanyu</creator><creator>Wen, Jifang</creator><creator>Zhou, Jianhua</creator><general>Blackwell Publishing Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>201110</creationdate><title>The expression of ADAM23 and its correlation with promoter methylation in non-small-cell lung carcinoma</title><author>Hu, Chunyan ; Lv, Hui ; Pan, Guoqing ; Cao, Huiqiu ; Deng, Zhenghao ; Hu, Chuanyu ; Wen, Jifang ; Zhou, Jianhua</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5436-c348aa0d3dd58ef858be6f96f73c9eb53fcf61d4460df5436d34be46100cb8a53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>5-aza-2'-deoxycytidine</topic><topic>5-Aza-2′-dC</topic><topic>ADAM Proteins - genetics</topic><topic>ADAM Proteins - metabolism</topic><topic>ADAM23 gene</topic><topic>Adult</topic><topic>Aged</topic><topic>Biomarkers, Tumor - genetics</topic><topic>Biomarkers, Tumor - metabolism</topic><topic>cancer cells lines</topic><topic>Carcinoma, Non-Small-Cell Lung - genetics</topic><topic>Carcinoma, Non-Small-Cell Lung - metabolism</topic><topic>Carcinoma, Non-Small-Cell Lung - pathology</topic><topic>Case-Control Studies</topic><topic>Cell Line, Tumor</topic><topic>Gene Expression Regulation, Neoplastic - physiology</topic><topic>Humans</topic><topic>Lung - metabolism</topic><topic>Lung - pathology</topic><topic>Lung Neoplasms - genetics</topic><topic>Lung Neoplasms - metabolism</topic><topic>Lung Neoplasms - pathology</topic><topic>Methylation</topic><topic>methylation-specific PCR</topic><topic>Middle Aged</topic><topic>non-small-cell lung carcinoma</topic><topic>Original</topic><topic>Promoter Regions, Genetic - genetics</topic><topic>Promoter Regions, Genetic - physiology</topic><topic>RNA, Messenger - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hu, Chunyan</creatorcontrib><creatorcontrib>Lv, Hui</creatorcontrib><creatorcontrib>Pan, Guoqing</creatorcontrib><creatorcontrib>Cao, Huiqiu</creatorcontrib><creatorcontrib>Deng, Zhenghao</creatorcontrib><creatorcontrib>Hu, Chuanyu</creatorcontrib><creatorcontrib>Wen, Jifang</creatorcontrib><creatorcontrib>Zhou, Jianhua</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>International journal of experimental pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hu, Chunyan</au><au>Lv, Hui</au><au>Pan, Guoqing</au><au>Cao, Huiqiu</au><au>Deng, Zhenghao</au><au>Hu, Chuanyu</au><au>Wen, Jifang</au><au>Zhou, Jianhua</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The expression of ADAM23 and its correlation with promoter methylation in non-small-cell lung carcinoma</atitle><jtitle>International journal of experimental pathology</jtitle><addtitle>Int J Exp Pathol</addtitle><date>2011-10</date><risdate>2011</risdate><volume>92</volume><issue>5</issue><spage>333</spage><epage>339</epage><pages>333-339</pages><issn>0959-9673</issn><eissn>1365-2613</eissn><abstract>Summary
ADAM23, a member of a disintegrin and metalloprotease (ADAM) family, has been reported to be expressed in several types of tumours. The exact role of ADAM23 and the possible mechanisms in which it is involved in non‐small‐cell lung carcinoma (NSCLC) remains unclear. Therefore, this study was designed to explore the expression of ADAM23 and its correlation with promoter methylation in NSCLC. Immunohistochemistry and RT‐PCR together with Western blotting methods were used to analyse the expression of ADAM23 in 52 cancer tissue samples and eight benign pulmonary lesions as well as four cell lines. The methylated status of ADAM23 gene was determined with methylation‐specific PCR (MSP). The results of immunohistochemistry showed that the expression of ADAM23 protein was lower in NSCLC than that in corresponding normal tissues and benign pulmonary lesions (38.5%vs. 86.5% and 87.5%, P < 0.05), and decreased as NSCLC progressed. Meanwhile, methylation of ADAM23 gene was observed in 21 of 52 NSCLC tissues (40.4%), much higher than that of adjacent normal tissues (7.6%) and benign pulmonary lesions (0/8). In the cancer tissues of ADAM23‐negative samples, the rate of ADAM23 gene methylation was 50.3% (17/32). ADAM23 expression and its promoter methylation were negatively associated (r = −0.328, P = 0.017). Moreover, weak expression of ADAM23 in methylated cancer cells increased after treatment with 5‐aza‐2′‐deoxycytidine (5‐Aza‐2′‐dC), confirming that methylation was responsible for the gene downregulation. Our results demonstrate that the expression level of ADAM23 is likely to be involved in the progression of NSCLC and its downregulation is probably correlated with promoter methylation. These findings may provide potential diagnostic and prognostic information about NSCLC.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>21429053</pmid><doi>10.1111/j.1365-2613.2011.00766.x</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | 5-aza-2'-deoxycytidine 5-Aza-2′-dC ADAM Proteins - genetics ADAM Proteins - metabolism ADAM23 gene Adult Aged Biomarkers, Tumor - genetics Biomarkers, Tumor - metabolism cancer cells lines Carcinoma, Non-Small-Cell Lung - genetics Carcinoma, Non-Small-Cell Lung - metabolism Carcinoma, Non-Small-Cell Lung - pathology Case-Control Studies Cell Line, Tumor Gene Expression Regulation, Neoplastic - physiology Humans Lung - metabolism Lung - pathology Lung Neoplasms - genetics Lung Neoplasms - metabolism Lung Neoplasms - pathology Methylation methylation-specific PCR Middle Aged non-small-cell lung carcinoma Original Promoter Regions, Genetic - genetics Promoter Regions, Genetic - physiology RNA, Messenger - metabolism |
| title | The expression of ADAM23 and its correlation with promoter methylation in non-small-cell lung carcinoma |
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