Magnetic Manipulation of Nanorods in the Nucleus of Living Cells

The organization of chromatin in the cell nucleus is crucial for gene expression regulation. However, physically probing the nuclear interior is challenging because high forces have to be applied using minimally invasive techniques. Here, magnetic nanorods embedded in the nucleus of living cells are...

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Veröffentlicht in:Biophysical journal 2011-10, Vol.101 (8), p.1880-1886
Hauptverfasser: Celedon, Alfredo, Hale, Christopher M., Wirtz, Denis
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creator Celedon, Alfredo
Hale, Christopher M.
Wirtz, Denis
description The organization of chromatin in the cell nucleus is crucial for gene expression regulation. However, physically probing the nuclear interior is challenging because high forces have to be applied using minimally invasive techniques. Here, magnetic nanorods embedded in the nucleus of living cells are subjected to controlled rotational forces, producing micron-sized displacements in the nuclear interior. The resulting time-dependent rotation of the nanorods is analyzed in terms of viscoelastic parameters of the nucleus, in wild-type and Lamin A/C deficient cells. This method and analysis reveal that Lamin A/C knockout, together perhaps with other changes that result from the knockout, induce significant decreases in the nuclear viscosity and elasticity.
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source MEDLINE; Cell Press Free Archives; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; ScienceDirect Journals (5 years ago - present); PubMed Central
subjects Animals
Biomechanical Phenomena
Cell Line
Cell Nucleus - metabolism
Cell Survival
Cells
Cellular Biophysics and Electrophysiology
Chromatin
Chromatin - metabolism
Elasticity
Gene expression
gene expression regulation
Gene Knockout Techniques
Lamin Type A - deficiency
Lamin Type A - genetics
Magnetic Fields
Mice
nanorods
Nanotubes
Rotation
Viscoelasticity
Viscosity
title Magnetic Manipulation of Nanorods in the Nucleus of Living Cells
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