Therapeutic hypothermia alters microRNA responses to traumatic brain injury in rats
Therapeutic hypothermia promotes protection after traumatic brain injury (TBI). The mechanisms underlying hypothermic protection are multifactorial and may include the modulation of microRNA (miRNA) expression after trauma. We utilized microarrays to examine the effects of posttraumatic hypothermia...
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| Veröffentlicht in: | Journal of cerebral blood flow and metabolism 2011-09, Vol.31 (9), p.1897-1907 |
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| creator | Truettner, Jessie S Alonso, Ofelia F Bramlett, Helen M Dietrich, W Dalton |
| description | Therapeutic hypothermia promotes protection after traumatic brain injury (TBI). The mechanisms underlying hypothermic protection are multifactorial and may include the modulation of microRNA (miRNA) expression after trauma. We utilized microarrays to examine the effects of posttraumatic hypothermia on the expression of 388 rat miRNAs. Animals were subjected to sham or moderate fluid percussion brain injury, followed by 4 hours of hypothermia (33°C) or normothermia (37°C) and euthanized at 7 or 24 hours. At 7hours, 47 miRNAs were significantly different (P < 0.05) between TBI and sham (15 higher in TBI and 31 lower). After 24hours, 15 miRNAs differed by P < 0.05 (7 higher and 9 lower). The expression of miRNAs was altered by posttraumatic hypothermia. At 7hours, seven were higher in hypothermia than normothermia and five were lower. Some miRNAs (e.g., miR-874 and miR-451) showed the most difference with hypothermia, with changes verified by quantitative reverse transcriptase-PCR. Regionally specific miRNAs also showed responses to TBI and hypothermia treatments by in situ hybridization. In addition, in vitro neuronal stretch injury studies showed similar temperature-sensitive responses to specific miRNAs. These novel data indicate that the reported beneficial effects of early hypothermia on traumatic outcome may include temperature-sensitive miRNAs involved in basic cell-processing events. |
| doi_str_mv | 10.1038/jcbfm.2011.33 |
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The mechanisms underlying hypothermic protection are multifactorial and may include the modulation of microRNA (miRNA) expression after trauma. We utilized microarrays to examine the effects of posttraumatic hypothermia on the expression of 388 rat miRNAs. Animals were subjected to sham or moderate fluid percussion brain injury, followed by 4 hours of hypothermia (33°C) or normothermia (37°C) and euthanized at 7 or 24 hours. At 7hours, 47 miRNAs were significantly different (P < 0.05) between TBI and sham (15 higher in TBI and 31 lower). After 24hours, 15 miRNAs differed by P < 0.05 (7 higher and 9 lower). The expression of miRNAs was altered by posttraumatic hypothermia. At 7hours, seven were higher in hypothermia than normothermia and five were lower. Some miRNAs (e.g., miR-874 and miR-451) showed the most difference with hypothermia, with changes verified by quantitative reverse transcriptase-PCR. Regionally specific miRNAs also showed responses to TBI and hypothermia treatments by in situ hybridization. In addition, in vitro neuronal stretch injury studies showed similar temperature-sensitive responses to specific miRNAs. These novel data indicate that the reported beneficial effects of early hypothermia on traumatic outcome may include temperature-sensitive miRNAs involved in basic cell-processing events.</description><identifier>ISSN: 0271-678X</identifier><identifier>EISSN: 1559-7016</identifier><identifier>DOI: 10.1038/jcbfm.2011.33</identifier><identifier>PMID: 21505482</identifier><identifier>CODEN: JCBMDN</identifier><language>eng</language><publisher>London, England: SAGE Publications</publisher><subject>Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy ; Animals ; Biological and medical sciences ; Brain - metabolism ; Brain Injuries - genetics ; Brain Injuries - therapy ; Cells, Cultured ; Emergency and intensive care: comas and nervous system diseases ; Gene Expression Regulation ; Hypothermia, Induced ; In Situ Hybridization ; Intensive care medicine ; Male ; Medical sciences ; MicroRNAs - genetics ; Neurology ; Neurons - metabolism ; Original ; Rats ; Rats, Sprague-Dawley ; Reverse Transcriptase Polymerase Chain Reaction ; Vascular diseases and vascular malformations of the nervous system</subject><ispartof>Journal of cerebral blood flow and metabolism, 2011-09, Vol.31 (9), p.1897-1907</ispartof><rights>2011 ISCBFM</rights><rights>2015 INIST-CNRS</rights><rights>Copyright Nature Publishing Group Sep 2011</rights><rights>Copyright © 2011 International Society for Cerebral Blood Flow & Metabolism, Inc. 2011 International Society for Cerebral Blood Flow & Metabolism, Inc.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c676t-ff4f7d53697c199064a842bad3b6ddb5d7081194fed2cccf2a807c43d1ef03fd3</citedby><cites>FETCH-LOGICAL-c676t-ff4f7d53697c199064a842bad3b6ddb5d7081194fed2cccf2a807c43d1ef03fd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3185878/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3185878/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,21819,27924,27925,43621,43622,53791,53793</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=24516205$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21505482$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Truettner, Jessie S</creatorcontrib><creatorcontrib>Alonso, Ofelia F</creatorcontrib><creatorcontrib>Bramlett, Helen M</creatorcontrib><creatorcontrib>Dietrich, W Dalton</creatorcontrib><title>Therapeutic hypothermia alters microRNA responses to traumatic brain injury in rats</title><title>Journal of cerebral blood flow and metabolism</title><addtitle>J Cereb Blood Flow Metab</addtitle><description>Therapeutic hypothermia promotes protection after traumatic brain injury (TBI). The mechanisms underlying hypothermic protection are multifactorial and may include the modulation of microRNA (miRNA) expression after trauma. We utilized microarrays to examine the effects of posttraumatic hypothermia on the expression of 388 rat miRNAs. Animals were subjected to sham or moderate fluid percussion brain injury, followed by 4 hours of hypothermia (33°C) or normothermia (37°C) and euthanized at 7 or 24 hours. At 7hours, 47 miRNAs were significantly different (P < 0.05) between TBI and sham (15 higher in TBI and 31 lower). After 24hours, 15 miRNAs differed by P < 0.05 (7 higher and 9 lower). The expression of miRNAs was altered by posttraumatic hypothermia. At 7hours, seven were higher in hypothermia than normothermia and five were lower. Some miRNAs (e.g., miR-874 and miR-451) showed the most difference with hypothermia, with changes verified by quantitative reverse transcriptase-PCR. Regionally specific miRNAs also showed responses to TBI and hypothermia treatments by in situ hybridization. In addition, in vitro neuronal stretch injury studies showed similar temperature-sensitive responses to specific miRNAs. These novel data indicate that the reported beneficial effects of early hypothermia on traumatic outcome may include temperature-sensitive miRNAs involved in basic cell-processing events.</description><subject>Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Brain - metabolism</subject><subject>Brain Injuries - genetics</subject><subject>Brain Injuries - therapy</subject><subject>Cells, Cultured</subject><subject>Emergency and intensive care: comas and nervous system diseases</subject><subject>Gene Expression Regulation</subject><subject>Hypothermia, Induced</subject><subject>In Situ Hybridization</subject><subject>Intensive care medicine</subject><subject>Male</subject><subject>Medical sciences</subject><subject>MicroRNAs - genetics</subject><subject>Neurology</subject><subject>Neurons - metabolism</subject><subject>Original</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Vascular diseases and vascular malformations of the nervous system</subject><issn>0271-678X</issn><issn>1559-7016</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqF0duL1DAUB-AgijuuPvoqVRBB6JhL06QvwrJ4g0VBV_AtnOayk6FNa9IK89-bzoyzKohPIeTLLyfnIPSY4DXBTL7a6tb1a4oJWTN2B60I500pMKnvohWmgpS1kN_O0IOUthhjyTi_j84o4ZhXkq7Ql-uNjTDaefK62OzGYcr73kMB3WRjKnqv4_D540URbRqHkGwqpqGYIsw9LFfaCD4UPmznuMtLEWFKD9E9B12yj47rOfr69s315fvy6tO7D5cXV6WuRT2VzlVOGM7qRmjSNLiuQFa0BcPa2piWG4ElIU3lrKFaa0dBYqErZoh1mDnDztHrQ-44t7012oZcV6fG6HuIOzWAV3-eBL9RN8MPxYjkUsgc8OIYEIfvs02T6n3Stusg2GFOqqklEdmS_0opBccUM5bls7_kdphjyH1QsqmIoEwsceUB5eamFK07FU2wWsaq9mNVy1jVPvTJ7z896V9zzOD5EUDS0LkIQft06ypOaop5di8PLsGNva3sX68-PeAA0xztKW2vFpTNTxkZxoM</recordid><startdate>20110901</startdate><enddate>20110901</enddate><creator>Truettner, Jessie S</creator><creator>Alonso, Ofelia F</creator><creator>Bramlett, Helen M</creator><creator>Dietrich, W Dalton</creator><general>SAGE Publications</general><general>Nature Publishing Group</general><general>Sage Publications Ltd</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>7TK</scope><scope>5PM</scope></search><sort><creationdate>20110901</creationdate><title>Therapeutic hypothermia alters microRNA responses to traumatic brain injury in rats</title><author>Truettner, Jessie S ; Alonso, Ofelia F ; Bramlett, Helen M ; Dietrich, W Dalton</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c676t-ff4f7d53697c199064a842bad3b6ddb5d7081194fed2cccf2a807c43d1ef03fd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Anesthesia. Intensive care medicine. Transfusions. 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The mechanisms underlying hypothermic protection are multifactorial and may include the modulation of microRNA (miRNA) expression after trauma. We utilized microarrays to examine the effects of posttraumatic hypothermia on the expression of 388 rat miRNAs. Animals were subjected to sham or moderate fluid percussion brain injury, followed by 4 hours of hypothermia (33°C) or normothermia (37°C) and euthanized at 7 or 24 hours. At 7hours, 47 miRNAs were significantly different (P < 0.05) between TBI and sham (15 higher in TBI and 31 lower). After 24hours, 15 miRNAs differed by P < 0.05 (7 higher and 9 lower). The expression of miRNAs was altered by posttraumatic hypothermia. At 7hours, seven were higher in hypothermia than normothermia and five were lower. Some miRNAs (e.g., miR-874 and miR-451) showed the most difference with hypothermia, with changes verified by quantitative reverse transcriptase-PCR. Regionally specific miRNAs also showed responses to TBI and hypothermia treatments by in situ hybridization. In addition, in vitro neuronal stretch injury studies showed similar temperature-sensitive responses to specific miRNAs. These novel data indicate that the reported beneficial effects of early hypothermia on traumatic outcome may include temperature-sensitive miRNAs involved in basic cell-processing events.</abstract><cop>London, England</cop><pub>SAGE Publications</pub><pmid>21505482</pmid><doi>10.1038/jcbfm.2011.33</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy Animals Biological and medical sciences Brain - metabolism Brain Injuries - genetics Brain Injuries - therapy Cells, Cultured Emergency and intensive care: comas and nervous system diseases Gene Expression Regulation Hypothermia, Induced In Situ Hybridization Intensive care medicine Male Medical sciences MicroRNAs - genetics Neurology Neurons - metabolism Original Rats Rats, Sprague-Dawley Reverse Transcriptase Polymerase Chain Reaction Vascular diseases and vascular malformations of the nervous system |
| title | Therapeutic hypothermia alters microRNA responses to traumatic brain injury in rats |
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