Automated polymerase chain reaction in capillary tubes with hot air
We describe a simple, compact, inexpensive thermal cycler that can be used for the polymerase chain reaction. Based on heat transfer with air to samples in sealed capillary tubes, the apparatus resembles a recirculating hair dryer. The temperature is regulated via thermocouple input to a programmabl...
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| Veröffentlicht in: | Nucleic acids research 1989-06, Vol.17 (11), p.4353-4357 |
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| container_end_page | 4357 |
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| container_issue | 11 |
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| container_title | Nucleic acids research |
| container_volume | 17 |
| creator | WITTWER, C. T FILLMORE, G. C HILLYARD, D. R |
| description | We describe a simple, compact, inexpensive thermal cycler that can be used for the polymerase chain reaction. Based on heat transfer with air to samples in sealed capillary tubes, the apparatus resembles a recirculating hair dryer. The temperature is regulated via thermocouple input to a programmable set-point process controller that provides proportional output to a solid state relay controlling a heating coil. For efficient cooling after the denaturation step, the controller activates a solenoid that opens a door to vent hot air and allows cool air to enter. Temperature-time profiles and amplification results approximate those obtained using water baths and microfuge tubes. |
| doi_str_mv | 10.1093/nar/17.11.4353 |
| format | Article |
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T</creatorcontrib><creatorcontrib>FILLMORE, G. C</creatorcontrib><creatorcontrib>HILLYARD, D. R</creatorcontrib><title>Automated polymerase chain reaction in capillary tubes with hot air</title><title>Nucleic acids research</title><addtitle>Nucleic Acids Res</addtitle><description>We describe a simple, compact, inexpensive thermal cycler that can be used for the polymerase chain reaction. Based on heat transfer with air to samples in sealed capillary tubes, the apparatus resembles a recirculating hair dryer. The temperature is regulated via thermocouple input to a programmable set-point process controller that provides proportional output to a solid state relay controlling a heating coil. For efficient cooling after the denaturation step, the controller activates a solenoid that opens a door to vent hot air and allows cool air to enter. 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Psychology</subject><subject>Gene Amplification</subject><subject>General aspects, investigation methods</subject><subject>Glass</subject><subject>Globins - genetics</subject><subject>Hot Temperature</subject><subject>Humans</subject><subject>Kinetics</subject><subject>Nucleic Acid Denaturation</subject><subject>Nucleic acids</subject><subject>Taq Polymerase</subject><issn>0305-1048</issn><issn>1362-4962</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1r3DAQxUVpSTdpr70VfCi9eTMjyZJ16CEszQcEemnPYizLXRXb2kpyQ_77OmRZ2lNOM_B-b5jHY-wDwhbBiMuZ0iXqLeJWika8YhsUitfSKP6abUBAUyPI9i07z_kXAEps5Bk741oCx3bDdldLiRMV31eHOD5OPlH2ldtTmKvkyZUQ52rdHR3COFJ6rMrS-Vw9hLKv9rFUFNI79magMfv3x3nBflx__b67re-_3dztru5rJ1sotUL0gJ2ERuhey963TU-kBXbOkB-k4Z0ynHPXm9Y0A0FvpHZKeeN1b0QrLtiX57uHpZt87_xcEo32kMK0PmYjBfu_Moe9_Rn_WIHaCLP6Px_9Kf5efC52Ctn5Ndbs45KtNtACV_xFEI1otDCwgttn0KWYc_LD6RkE-1SPXeuxqC2ifapnNXz8N8IJP_ax6p-OOmVH45BodiGfMC0UKGnEX3nbmR4</recordid><startdate>19890612</startdate><enddate>19890612</enddate><creator>WITTWER, C. 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Psychology</topic><topic>Gene Amplification</topic><topic>General aspects, investigation methods</topic><topic>Glass</topic><topic>Globins - genetics</topic><topic>Hot Temperature</topic><topic>Humans</topic><topic>Kinetics</topic><topic>Nucleic Acid Denaturation</topic><topic>Nucleic acids</topic><topic>Taq Polymerase</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>WITTWER, C. T</creatorcontrib><creatorcontrib>FILLMORE, G. C</creatorcontrib><creatorcontrib>HILLYARD, D. 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| fulltext | fulltext |
| identifier | ISSN: 0305-1048 |
| ispartof | Nucleic acids research, 1989-06, Vol.17 (11), p.4353-4357 |
| issn | 0305-1048 1362-4962 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_317939 |
| source | MEDLINE; PubMed Central; Oxford University Press Journals Digital Archive Legacy |
| subjects | Air Analytical, structural and metabolic biochemistry Autoanalysis - instrumentation Autoanalysis - methods Biological and medical sciences DNA, Bacterial - genetics DNA-Directed DNA Polymerase Fundamental and applied biological sciences. Psychology Gene Amplification General aspects, investigation methods Glass Globins - genetics Hot Temperature Humans Kinetics Nucleic Acid Denaturation Nucleic acids Taq Polymerase |
| title | Automated polymerase chain reaction in capillary tubes with hot air |
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