Perlecan/Hspg2 deficiency alters the pericellular space of the lacunocanalicular system surrounding osteocytic processes in cortical bone
Osteocytes project long, slender processes throughout the mineralized matrix of bone, where they connect and communicate with effector cells. The interconnected cellular projections form the functional lacunocanalicular system, allowing fluid to pass for cell‐to‐cell communication and nutrient and w...
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| Veröffentlicht in: | Journal of bone and mineral research 2011-03, Vol.26 (3), p.618-629 |
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| container_title | Journal of bone and mineral research |
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| creator | Thompson, William R Modla, Shannon Grindel, Brian J Czymmek, Kirk J Kirn‐Safran, Catherine B Wang, Liyun Duncan, Randall L Farach‐Carson, Mary C |
| description | Osteocytes project long, slender processes throughout the mineralized matrix of bone, where they connect and communicate with effector cells. The interconnected cellular projections form the functional lacunocanalicular system, allowing fluid to pass for cell‐to‐cell communication and nutrient and waste exchange. Prevention of mineralization in the pericellular space of the lacunocanalicular pericellular space is crucial for uninhibited interstitial fluid movement. Factors contributing to the ability of the pericellular space of the lacunocanalicular system to remain open and unmineralized are unclear. Immunofluorescence and immunogold localization by transmission electron microscopy demonstrated perlecan/Hspg2 signal localized to the osteocyte lacunocanalicular system of cortical bone, and this proteoglycan was found in the pericellular space of the lacunocanalicular system. In this study we examined osteocyte lacunocanalicular morphology in mice deficient in the large heparan sulfate proteoglycan perlecan/Hspg2 in this tissue. Ultrastructural measurements with electron microscopy of perlecan/Hspg2‐deficient mice demonstrated diminished osteocyte canalicular pericellular area, resulting from a reduction in the total canalicular area. Additionally, perlecan/Hspg2‐deficient mice showed decreased canalicular density and a reduced number of transverse tethering elements per canaliculus. These data indicated that perlecan/Hspg2 contributed to the integrity of the osteocyte lacunocanalicular system by maintaining the size of the pericellular space, an essential task to promote uninhibited interstitial fluid movement in this mechanosensitive environment. This work thus identified a new barrier function for perlecan/Hspg2 in murine cortical bone. © 2011 American Society for Bone and Mineral Research. |
| doi_str_mv | 10.1002/jbmr.236 |
| format | Article |
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The interconnected cellular projections form the functional lacunocanalicular system, allowing fluid to pass for cell‐to‐cell communication and nutrient and waste exchange. Prevention of mineralization in the pericellular space of the lacunocanalicular pericellular space is crucial for uninhibited interstitial fluid movement. Factors contributing to the ability of the pericellular space of the lacunocanalicular system to remain open and unmineralized are unclear. Immunofluorescence and immunogold localization by transmission electron microscopy demonstrated perlecan/Hspg2 signal localized to the osteocyte lacunocanalicular system of cortical bone, and this proteoglycan was found in the pericellular space of the lacunocanalicular system. In this study we examined osteocyte lacunocanalicular morphology in mice deficient in the large heparan sulfate proteoglycan perlecan/Hspg2 in this tissue. Ultrastructural measurements with electron microscopy of perlecan/Hspg2‐deficient mice demonstrated diminished osteocyte canalicular pericellular area, resulting from a reduction in the total canalicular area. Additionally, perlecan/Hspg2‐deficient mice showed decreased canalicular density and a reduced number of transverse tethering elements per canaliculus. These data indicated that perlecan/Hspg2 contributed to the integrity of the osteocyte lacunocanalicular system by maintaining the size of the pericellular space, an essential task to promote uninhibited interstitial fluid movement in this mechanosensitive environment. This work thus identified a new barrier function for perlecan/Hspg2 in murine cortical bone. © 2011 American Society for Bone and Mineral Research.</description><identifier>ISSN: 0884-0431</identifier><identifier>EISSN: 1523-4681</identifier><identifier>DOI: 10.1002/jbmr.236</identifier><identifier>PMID: 20814969</identifier><identifier>CODEN: JBMREJ</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Animals ; Biological and medical sciences ; Bone and Bones - metabolism ; Bone and Bones - pathology ; CORTICAL BONE ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation ; HEPARAN SULFATE ; Heparan Sulfate Proteoglycans - deficiency ; Heparan Sulfate Proteoglycans - genetics ; Heparan Sulfate Proteoglycans - metabolism ; Intracellular Space - metabolism ; LACUNOCANALICULAR SYSTEM ; MECHANOSENSING ; Mice ; Molecular Weight ; Original ; OSTEOCYTE ; Osteocytes - metabolism ; Osteocytes - ultrastructure ; PERLECAN/HSPG2 ; Protein Transport ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Skeleton and joints ; Vertebrates: osteoarticular system, musculoskeletal system</subject><ispartof>Journal of bone and mineral research, 2011-03, Vol.26 (3), p.618-629</ispartof><rights>Copyright © 2011 American Society for Bone and Mineral Research</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2011 American Society for Bone and Mineral Research.</rights><rights>Copyright © 2011 American Society for Bone and Mineral Research 2011</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5986-abb798ff44652a7d2dde45571a1b6779969d38cda7005a3ffa84955545d15ec3</citedby><cites>FETCH-LOGICAL-c5986-abb798ff44652a7d2dde45571a1b6779969d38cda7005a3ffa84955545d15ec3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjbmr.236$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjbmr.236$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>230,314,777,781,882,1412,27905,27906,45555,45556</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=24288760$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20814969$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Thompson, William R</creatorcontrib><creatorcontrib>Modla, Shannon</creatorcontrib><creatorcontrib>Grindel, Brian J</creatorcontrib><creatorcontrib>Czymmek, Kirk J</creatorcontrib><creatorcontrib>Kirn‐Safran, Catherine B</creatorcontrib><creatorcontrib>Wang, Liyun</creatorcontrib><creatorcontrib>Duncan, Randall L</creatorcontrib><creatorcontrib>Farach‐Carson, Mary C</creatorcontrib><title>Perlecan/Hspg2 deficiency alters the pericellular space of the lacunocanalicular system surrounding osteocytic processes in cortical bone</title><title>Journal of bone and mineral research</title><addtitle>J Bone Miner Res</addtitle><description>Osteocytes project long, slender processes throughout the mineralized matrix of bone, where they connect and communicate with effector cells. The interconnected cellular projections form the functional lacunocanalicular system, allowing fluid to pass for cell‐to‐cell communication and nutrient and waste exchange. Prevention of mineralization in the pericellular space of the lacunocanalicular pericellular space is crucial for uninhibited interstitial fluid movement. Factors contributing to the ability of the pericellular space of the lacunocanalicular system to remain open and unmineralized are unclear. Immunofluorescence and immunogold localization by transmission electron microscopy demonstrated perlecan/Hspg2 signal localized to the osteocyte lacunocanalicular system of cortical bone, and this proteoglycan was found in the pericellular space of the lacunocanalicular system. In this study we examined osteocyte lacunocanalicular morphology in mice deficient in the large heparan sulfate proteoglycan perlecan/Hspg2 in this tissue. Ultrastructural measurements with electron microscopy of perlecan/Hspg2‐deficient mice demonstrated diminished osteocyte canalicular pericellular area, resulting from a reduction in the total canalicular area. Additionally, perlecan/Hspg2‐deficient mice showed decreased canalicular density and a reduced number of transverse tethering elements per canaliculus. These data indicated that perlecan/Hspg2 contributed to the integrity of the osteocyte lacunocanalicular system by maintaining the size of the pericellular space, an essential task to promote uninhibited interstitial fluid movement in this mechanosensitive environment. This work thus identified a new barrier function for perlecan/Hspg2 in murine cortical bone. © 2011 American Society for Bone and Mineral Research.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Bone and Bones - metabolism</subject><subject>Bone and Bones - pathology</subject><subject>CORTICAL BONE</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation</subject><subject>HEPARAN SULFATE</subject><subject>Heparan Sulfate Proteoglycans - deficiency</subject><subject>Heparan Sulfate Proteoglycans - genetics</subject><subject>Heparan Sulfate Proteoglycans - metabolism</subject><subject>Intracellular Space - metabolism</subject><subject>LACUNOCANALICULAR SYSTEM</subject><subject>MECHANOSENSING</subject><subject>Mice</subject><subject>Molecular Weight</subject><subject>Original</subject><subject>OSTEOCYTE</subject><subject>Osteocytes - metabolism</subject><subject>Osteocytes - ultrastructure</subject><subject>PERLECAN/HSPG2</subject><subject>Protein Transport</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Skeleton and joints</subject><subject>Vertebrates: osteoarticular system, musculoskeletal system</subject><issn>0884-0431</issn><issn>1523-4681</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFktGK1TAQhoso7nEVfAIJiOhNd5M0SdMbQRd1lRVF9j6k6fRsDmlSk1bpI_jWpp7jrgrqVWDmyz_MP39RPCT4hGBMT3ftEE9oJW4VG8JpVTIhye1ig6VkJWYVOSrupbTDGAsuxN3iiGJJWCOaTfHtI0QHRvvT8zRuKeqgt8aCNwvSboKY0HQFaIRoDTg3Ox1RGrUBFPofHafN7EP-r501-_aSJhhQmmMMs--s36KQK8EskzVojMFASpCQ9ciEmGvaoTZ4uF_c6bVL8ODwHheXr19dnp2XFx_evD17cVEa3khR6ratG9n3jAlOdd3RrgPGeU00aUVdN3mrrpKm0zXGXFd9ryVrOOeMd4SDqY6L53vZcW4H6Az4KWqnxmgHHRcVtFW_d7y9UtvwRVWkbmjDssDTg0AMn2dIkxpsWs3RHsKclBS4kjWt8f9JXlHKGkkz-eyfJMmCMqsSntHHf6C7MMds_0qJ7AkhDN8ImhhSitBfL0iwWiOj1sioHJmMPvrVkGvwZ0Yy8OQA6JSP1UftjU03HKNS1mKdWe65r9bB8teB6t3L95_Wwd8Bgjra9w</recordid><startdate>201103</startdate><enddate>201103</enddate><creator>Thompson, William R</creator><creator>Modla, Shannon</creator><creator>Grindel, Brian J</creator><creator>Czymmek, Kirk J</creator><creator>Kirn‐Safran, Catherine B</creator><creator>Wang, Liyun</creator><creator>Duncan, Randall L</creator><creator>Farach‐Carson, Mary C</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley</general><general>Wiley Subscription Services, Inc</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7TS</scope><scope>K9.</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>201103</creationdate><title>Perlecan/Hspg2 deficiency alters the pericellular space of the lacunocanalicular system surrounding osteocytic processes in cortical bone</title><author>Thompson, William R ; Modla, Shannon ; Grindel, Brian J ; Czymmek, Kirk J ; Kirn‐Safran, Catherine B ; Wang, Liyun ; Duncan, Randall L ; Farach‐Carson, Mary C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5986-abb798ff44652a7d2dde45571a1b6779969d38cda7005a3ffa84955545d15ec3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Bone and Bones - metabolism</topic><topic>Bone and Bones - pathology</topic><topic>CORTICAL BONE</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation</topic><topic>HEPARAN SULFATE</topic><topic>Heparan Sulfate Proteoglycans - deficiency</topic><topic>Heparan Sulfate Proteoglycans - genetics</topic><topic>Heparan Sulfate Proteoglycans - metabolism</topic><topic>Intracellular Space - metabolism</topic><topic>LACUNOCANALICULAR SYSTEM</topic><topic>MECHANOSENSING</topic><topic>Mice</topic><topic>Molecular Weight</topic><topic>Original</topic><topic>OSTEOCYTE</topic><topic>Osteocytes - metabolism</topic><topic>Osteocytes - ultrastructure</topic><topic>PERLECAN/HSPG2</topic><topic>Protein Transport</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Skeleton and joints</topic><topic>Vertebrates: osteoarticular system, musculoskeletal system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Thompson, William R</creatorcontrib><creatorcontrib>Modla, Shannon</creatorcontrib><creatorcontrib>Grindel, Brian J</creatorcontrib><creatorcontrib>Czymmek, Kirk J</creatorcontrib><creatorcontrib>Kirn‐Safran, Catherine B</creatorcontrib><creatorcontrib>Wang, Liyun</creatorcontrib><creatorcontrib>Duncan, Randall L</creatorcontrib><creatorcontrib>Farach‐Carson, Mary C</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Physical Education Index</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of bone and mineral research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Thompson, William R</au><au>Modla, Shannon</au><au>Grindel, Brian J</au><au>Czymmek, Kirk J</au><au>Kirn‐Safran, Catherine B</au><au>Wang, Liyun</au><au>Duncan, Randall L</au><au>Farach‐Carson, Mary C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Perlecan/Hspg2 deficiency alters the pericellular space of the lacunocanalicular system surrounding osteocytic processes in cortical bone</atitle><jtitle>Journal of bone and mineral research</jtitle><addtitle>J Bone Miner Res</addtitle><date>2011-03</date><risdate>2011</risdate><volume>26</volume><issue>3</issue><spage>618</spage><epage>629</epage><pages>618-629</pages><issn>0884-0431</issn><eissn>1523-4681</eissn><coden>JBMREJ</coden><abstract>Osteocytes project long, slender processes throughout the mineralized matrix of bone, where they connect and communicate with effector cells. The interconnected cellular projections form the functional lacunocanalicular system, allowing fluid to pass for cell‐to‐cell communication and nutrient and waste exchange. Prevention of mineralization in the pericellular space of the lacunocanalicular pericellular space is crucial for uninhibited interstitial fluid movement. Factors contributing to the ability of the pericellular space of the lacunocanalicular system to remain open and unmineralized are unclear. Immunofluorescence and immunogold localization by transmission electron microscopy demonstrated perlecan/Hspg2 signal localized to the osteocyte lacunocanalicular system of cortical bone, and this proteoglycan was found in the pericellular space of the lacunocanalicular system. In this study we examined osteocyte lacunocanalicular morphology in mice deficient in the large heparan sulfate proteoglycan perlecan/Hspg2 in this tissue. Ultrastructural measurements with electron microscopy of perlecan/Hspg2‐deficient mice demonstrated diminished osteocyte canalicular pericellular area, resulting from a reduction in the total canalicular area. Additionally, perlecan/Hspg2‐deficient mice showed decreased canalicular density and a reduced number of transverse tethering elements per canaliculus. These data indicated that perlecan/Hspg2 contributed to the integrity of the osteocyte lacunocanalicular system by maintaining the size of the pericellular space, an essential task to promote uninhibited interstitial fluid movement in this mechanosensitive environment. This work thus identified a new barrier function for perlecan/Hspg2 in murine cortical bone. © 2011 American Society for Bone and Mineral Research.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>20814969</pmid><doi>10.1002/jbmr.236</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Oxford University Press Journals All Titles (1996-Current); EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Animals Biological and medical sciences Bone and Bones - metabolism Bone and Bones - pathology CORTICAL BONE Fundamental and applied biological sciences. Psychology Gene Expression Regulation HEPARAN SULFATE Heparan Sulfate Proteoglycans - deficiency Heparan Sulfate Proteoglycans - genetics Heparan Sulfate Proteoglycans - metabolism Intracellular Space - metabolism LACUNOCANALICULAR SYSTEM MECHANOSENSING Mice Molecular Weight Original OSTEOCYTE Osteocytes - metabolism Osteocytes - ultrastructure PERLECAN/HSPG2 Protein Transport RNA, Messenger - genetics RNA, Messenger - metabolism Skeleton and joints Vertebrates: osteoarticular system, musculoskeletal system |
| title | Perlecan/Hspg2 deficiency alters the pericellular space of the lacunocanalicular system surrounding osteocytic processes in cortical bone |
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