Targeted Mutagenesis of Duplicated Genes in Soybean with Zinc-Finger Nucleases

We performed targeted mutagenesis of a transgene and nine endogenous soybean (Glycine max) genes using zinc-finger nucleases (ZFNs). A suite of ZFNs were engineered by the recently described context-dependent assembly platform—a rapid, open-source method for generating zinc-finger arrays. Specific Z...

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Veröffentlicht in:	Plant physiology (Bethesda) 2011-06, Vol.156 (2), p.466-473
Hauptverfasser:	Curtin, Shaun J., Zhang, Feng, Sander, Jeffry D., Haun, William J., Starker, Colby, Baltes, Nicholas J., Reyon, Deepak, Dahlborg, Elizabeth J., Goodwin, Mathew J., Coffman, Andrew P., Dobbs, Drena, Joung, J. Keith, Voytas, Daniel F., Stupar, Robert M.
Format:	Artikel
Sprache:	eng
Schlagworte:	Base Sequence Biological and medical sciences BREAKTHROUGH TECHNOLOGIES Endonucleases - chemistry Endonucleases - metabolism Fundamental and applied biological sciences. Psychology Genes Genes, Duplicate - genetics Genes, Plant - genetics Genetic engineering Genetic mutation Genetic Techniques Genomes Glycine max - genetics Green Fluorescent Proteins - metabolism Inheritance Patterns - genetics Internet Molecular Sequence Data Mutagenesis Mutagenesis - genetics Mutation - genetics Plant physiology and development Plant Roots - genetics Plants Polymerase Chain Reaction Soybeans Transgenes Transgenes - genetics Zinc Zinc Fingers - genetics
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