A novel fluorescence-based assay for the rapid detection and quantification of cellular deoxyribonucleoside triphosphates

Current methods for measuring deoxyribonucleoside triphosphates (dNTPs) employ reagent and labor-intensive assays utilizing radioisotopes in DNA polymerase-based assays and/or chromatography-based approaches. We have developed a rapid and sensitive 96-well fluorescence-based assay to quantify cellul...

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Veröffentlicht in:	Nucleic acids research 2011-09, Vol.39 (17), p.e112-e112
Hauptverfasser:	Wilson, Peter M., LaBonte, Melissa J., Russell, Jared, Louie, Stan, Ghobrial, Andrew A., Ladner, Robert D.
Format:	Artikel
Sprache:	eng
Schlagworte:	Deoxyribonucleotides - analysis Deoxyuracil Nucleotides - analysis DNA-Directed DNA Polymerase Fluorescence Resonance Energy Transfer HCT116 Cells Humans Methods Online Nucleic Acid Hybridization - methods Oligonucleotide Probes Polymerase Chain Reaction Ribonucleotides - chemistry Templates, Genetic
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