DNA ligase activity in human cell lines from normal donors and Bloom's syndrome patients
DNA ligase activity was studied in several untransformed or virus-transformed human cell lines from normal donors and from Bloom's syndrome (BS) patients. This proneness genetic disease is characterized by several cytological abnormalities and cancer proneness and, recently, some transformed ce...
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| Veröffentlicht in: | Nucleic acids research 1989-04, Vol.17 (8), p.3091-3106 |
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| container_title | Nucleic acids research |
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| creator | MEZZINA, M NARDELLI, J NOCENTINI, S RENAULT, G SARASIN, A |
| description | DNA ligase activity was studied in several untransformed or virus-transformed human cell lines from normal donors and from Bloom's syndrome (BS) patients. This proneness genetic disease is characterized by several cytological abnormalities and cancer proneness and, recently, some transformed cell lines from these patients were described to present a reduced activity of DNA ligase I. Results presented in this work indicate that: (i) the total DNA ligase activity in crude extract from untransformed or transformed cell lines from several BS patients was significantly higher than in control cells; (ii) the partial purification of the enzyme after gel filtration on fast protein liquid chromatography of crude extracts from lymphoblastoid BS cells showed that the enzyme activity was eluted in a major 180 kDa form in which activity was higher than in control cells; (iii) the activity gel analysis of these enzyme fractions revealed that DNA ligase of human cells was correlated to a major 130 kDa polypeptide and, in BS cells, the extent of the activity of this band was equal or higher than that in control untransformed or transformed cells. |
| doi_str_mv | 10.1093/nar/17.8.3091 |
| format | Article |
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This proneness genetic disease is characterized by several cytological abnormalities and cancer proneness and, recently, some transformed cell lines from these patients were described to present a reduced activity of DNA ligase I. Results presented in this work indicate that: (i) the total DNA ligase activity in crude extract from untransformed or transformed cell lines from several BS patients was significantly higher than in control cells; (ii) the partial purification of the enzyme after gel filtration on fast protein liquid chromatography of crude extracts from lymphoblastoid BS cells showed that the enzyme activity was eluted in a major 180 kDa form in which activity was higher than in control cells; (iii) the activity gel analysis of these enzyme fractions revealed that DNA ligase of human cells was correlated to a major 130 kDa polypeptide and, in BS cells, the extent of the activity of this band was equal or higher than that in control untransformed or transformed cells.</description><identifier>ISSN: 0305-1048</identifier><identifier>EISSN: 1362-4962</identifier><identifier>DOI: 10.1093/nar/17.8.3091</identifier><identifier>PMID: 2726453</identifier><identifier>CODEN: NARHAD</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>Biological and medical sciences ; Bloom Syndrome - enzymology ; Cell Fractionation - methods ; Cell Line ; Chromatography, High Pressure Liquid ; Chromosome fragility (bloom syndrome, ataxia telangiectasia, fanconi anemia, x-linked mental retardation...) ; Deoxyribonucleases - metabolism ; DNA Ligases - isolation & purification ; DNA Ligases - metabolism ; Humans ; Medical genetics ; Medical sciences ; Molecular Weight ; Polynucleotide Ligases - metabolism</subject><ispartof>Nucleic acids research, 1989-04, Vol.17 (8), p.3091-3106</ispartof><rights>1990 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c472t-15c1b8a147da32d6ce02c93ccf4a0d19b35932dedacaec0b275717db7072436f3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC317716/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC317716/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=6916560$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2726453$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>MEZZINA, M</creatorcontrib><creatorcontrib>NARDELLI, J</creatorcontrib><creatorcontrib>NOCENTINI, S</creatorcontrib><creatorcontrib>RENAULT, G</creatorcontrib><creatorcontrib>SARASIN, A</creatorcontrib><title>DNA ligase activity in human cell lines from normal donors and Bloom's syndrome patients</title><title>Nucleic acids research</title><addtitle>Nucleic Acids Res</addtitle><description>DNA ligase activity was studied in several untransformed or virus-transformed human cell lines from normal donors and from Bloom's syndrome (BS) patients. This proneness genetic disease is characterized by several cytological abnormalities and cancer proneness and, recently, some transformed cell lines from these patients were described to present a reduced activity of DNA ligase I. Results presented in this work indicate that: (i) the total DNA ligase activity in crude extract from untransformed or transformed cell lines from several BS patients was significantly higher than in control cells; (ii) the partial purification of the enzyme after gel filtration on fast protein liquid chromatography of crude extracts from lymphoblastoid BS cells showed that the enzyme activity was eluted in a major 180 kDa form in which activity was higher than in control cells; (iii) the activity gel analysis of these enzyme fractions revealed that DNA ligase of human cells was correlated to a major 130 kDa polypeptide and, in BS cells, the extent of the activity of this band was equal or higher than that in control untransformed or transformed cells.</description><subject>Biological and medical sciences</subject><subject>Bloom Syndrome - enzymology</subject><subject>Cell Fractionation - methods</subject><subject>Cell Line</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Chromosome fragility (bloom syndrome, ataxia telangiectasia, fanconi anemia, x-linked mental retardation...)</subject><subject>Deoxyribonucleases - metabolism</subject><subject>DNA Ligases - isolation & purification</subject><subject>DNA Ligases - metabolism</subject><subject>Humans</subject><subject>Medical genetics</subject><subject>Medical sciences</subject><subject>Molecular Weight</subject><subject>Polynucleotide Ligases - metabolism</subject><issn>0305-1048</issn><issn>1362-4962</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkb1vFDEQxS0ECkegpERygaDai8cf63VBEQIkSFFoQKKzZr3exGjXPuy9SPffx6ecTlClGmveb2b89Ah5C2wNzIiziPkM9LpbC2bgGVmBaHkjTcufkxUTTDXAZPeSvCrlD2MgQckTcsI1b6USK_L7y805ncItFk_RLeE-LDsaIr3bzhip89NU1egLHXOaaUx5xokOqT4KxTjQz1NK88dCyy4OlfB0g0vwcSmvyYsRp-LfHOop-fXt68-Lq-b6x-X3i_PrxknNlwaUg75DkHpAwYfWecadEc6NEtkAphfK1L4f0KF3rOdaadBDr5nmUrSjOCWfHvdutv3sB1dvZ5zsJocZ884mDPZ_JYY7e5vurQCtoa3zHw7zOf3d-rLYOZS9b4w-bYvVnTGglHoSBKNkZZ_eCIp3UF1UsHkEXU6lZD8efw3M7rO1NVsL2nZ2n23l3_1r9Ugfwqz6-4OOxeE0ZowulCPWGmhVy8QD7lCt5A</recordid><startdate>19890425</startdate><enddate>19890425</enddate><creator>MEZZINA, M</creator><creator>NARDELLI, J</creator><creator>NOCENTINI, S</creator><creator>RENAULT, G</creator><creator>SARASIN, A</creator><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19890425</creationdate><title>DNA ligase activity in human cell lines from normal donors and Bloom's syndrome patients</title><author>MEZZINA, M ; NARDELLI, J ; NOCENTINI, S ; RENAULT, G ; SARASIN, A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c472t-15c1b8a147da32d6ce02c93ccf4a0d19b35932dedacaec0b275717db7072436f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>Biological and medical sciences</topic><topic>Bloom Syndrome - enzymology</topic><topic>Cell Fractionation - methods</topic><topic>Cell Line</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Chromosome fragility (bloom syndrome, ataxia telangiectasia, fanconi anemia, x-linked mental retardation...)</topic><topic>Deoxyribonucleases - metabolism</topic><topic>DNA Ligases - isolation & purification</topic><topic>DNA Ligases - metabolism</topic><topic>Humans</topic><topic>Medical genetics</topic><topic>Medical sciences</topic><topic>Molecular Weight</topic><topic>Polynucleotide Ligases - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>MEZZINA, M</creatorcontrib><creatorcontrib>NARDELLI, J</creatorcontrib><creatorcontrib>NOCENTINI, S</creatorcontrib><creatorcontrib>RENAULT, G</creatorcontrib><creatorcontrib>SARASIN, A</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Nucleic acids research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>MEZZINA, M</au><au>NARDELLI, J</au><au>NOCENTINI, S</au><au>RENAULT, G</au><au>SARASIN, A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>DNA ligase activity in human cell lines from normal donors and Bloom's syndrome patients</atitle><jtitle>Nucleic acids research</jtitle><addtitle>Nucleic Acids Res</addtitle><date>1989-04-25</date><risdate>1989</risdate><volume>17</volume><issue>8</issue><spage>3091</spage><epage>3106</epage><pages>3091-3106</pages><issn>0305-1048</issn><eissn>1362-4962</eissn><coden>NARHAD</coden><abstract>DNA ligase activity was studied in several untransformed or virus-transformed human cell lines from normal donors and from Bloom's syndrome (BS) patients. This proneness genetic disease is characterized by several cytological abnormalities and cancer proneness and, recently, some transformed cell lines from these patients were described to present a reduced activity of DNA ligase I. Results presented in this work indicate that: (i) the total DNA ligase activity in crude extract from untransformed or transformed cell lines from several BS patients was significantly higher than in control cells; (ii) the partial purification of the enzyme after gel filtration on fast protein liquid chromatography of crude extracts from lymphoblastoid BS cells showed that the enzyme activity was eluted in a major 180 kDa form in which activity was higher than in control cells; (iii) the activity gel analysis of these enzyme fractions revealed that DNA ligase of human cells was correlated to a major 130 kDa polypeptide and, in BS cells, the extent of the activity of this band was equal or higher than that in control untransformed or transformed cells.</abstract><cop>Oxford</cop><pub>Oxford University Press</pub><pmid>2726453</pmid><doi>10.1093/nar/17.8.3091</doi><tpages>16</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Nucleic acids research, 1989-04, Vol.17 (8), p.3091-3106 |
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| language | eng |
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| source | MEDLINE; Oxford University Press Archive; PubMed Central |
| subjects | Biological and medical sciences Bloom Syndrome - enzymology Cell Fractionation - methods Cell Line Chromatography, High Pressure Liquid Chromosome fragility (bloom syndrome, ataxia telangiectasia, fanconi anemia, x-linked mental retardation...) Deoxyribonucleases - metabolism DNA Ligases - isolation & purification DNA Ligases - metabolism Humans Medical genetics Medical sciences Molecular Weight Polynucleotide Ligases - metabolism |
| title | DNA ligase activity in human cell lines from normal donors and Bloom's syndrome patients |
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