Cell-selective metabolic labeling of proteins
Metabolic labeling of proteins with the methionine surrogate azidonorleucine can be targeted exclusively to specified cells through expression of a mutant methionyl-tRNA synthetase (MetRS). In complex cellular mixtures, proteins made in cells that express the mutant synthetase can be tagged with aff...
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| Veröffentlicht in: | Nature chemical biology 2009-10, Vol.5 (10), p.715-717 |
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| container_title | Nature chemical biology |
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| creator | Ngo, John T Champion, Julie A Mahdavi, Alborz Tanrikulu, I Caglar Beatty, Kimberly E Connor, Rebecca E Yoo, Tae Hyeon Dieterich, Daniela C Schuman, Erin M Tirrell, David A |
| description | Metabolic labeling of proteins with the methionine surrogate azidonorleucine can be targeted exclusively to specified cells through expression of a mutant methionyl-tRNA synthetase (MetRS). In complex cellular mixtures, proteins made in cells that express the mutant synthetase can be tagged with affinity reagents (for detection or enrichment) or fluorescent dyes (for imaging). Proteins made in cells that do not express the mutant synthetase are neither labeled nor detected. |
| doi_str_mv | 10.1038/nchembio.200 |
| format | Article |
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In complex cellular mixtures, proteins made in cells that express the mutant synthetase can be tagged with affinity reagents (for detection or enrichment) or fluorescent dyes (for imaging). Proteins made in cells that do not express the mutant synthetase are neither labeled nor detected.</description><identifier>ISSN: 1552-4450</identifier><identifier>EISSN: 1552-4469</identifier><identifier>DOI: 10.1038/nchembio.200</identifier><identifier>PMID: 19668194</identifier><language>eng</language><publisher>New York: Nature Publishing Group US</publisher><subject>Affinity Labels - metabolism ; Alanine - analogs & derivatives ; Alanine - metabolism ; Amino Acyl-tRNA Synthetases - genetics ; Amino Acyl-tRNA Synthetases - metabolism ; Animals ; Biochemical Engineering ; Biochemistry ; Bioorganic Chemistry ; brief-communication ; Cell Biology ; Cell Line ; Cellular biology ; Chemistry ; Chemistry and Materials Science ; Chemistry/Food Science ; Coculture Techniques ; Dyes ; Electrophoresis, Polyacrylamide Gel ; Escherichia coli - genetics ; Escherichia coli - metabolism ; Macrophages, Alveolar - metabolism ; Macrophages, Alveolar - microbiology ; Metabolism ; Methionine-tRNA Ligase - genetics ; Methionine-tRNA Ligase - metabolism ; Mice ; Mutagenesis, Site-Directed ; Mutants ; Mutation ; Norleucine - analogs & derivatives ; Norleucine - metabolism ; Protein Biosynthesis ; Proteins ; Proteins - metabolism ; Reagents</subject><ispartof>Nature chemical biology, 2009-10, Vol.5 (10), p.715-717</ispartof><rights>Springer Nature America, Inc. 2009</rights><rights>Copyright Nature Publishing Group Oct 2009</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1038/nchembio.200$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1038/nchembio.200$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>230,314,776,780,881,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19668194$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ngo, John T</creatorcontrib><creatorcontrib>Champion, Julie A</creatorcontrib><creatorcontrib>Mahdavi, Alborz</creatorcontrib><creatorcontrib>Tanrikulu, I Caglar</creatorcontrib><creatorcontrib>Beatty, Kimberly E</creatorcontrib><creatorcontrib>Connor, Rebecca E</creatorcontrib><creatorcontrib>Yoo, Tae Hyeon</creatorcontrib><creatorcontrib>Dieterich, Daniela C</creatorcontrib><creatorcontrib>Schuman, Erin M</creatorcontrib><creatorcontrib>Tirrell, David A</creatorcontrib><title>Cell-selective metabolic labeling of proteins</title><title>Nature chemical biology</title><addtitle>Nat Chem Biol</addtitle><addtitle>Nat Chem Biol</addtitle><description>Metabolic labeling of proteins with the methionine surrogate azidonorleucine can be targeted exclusively to specified cells through expression of a mutant methionyl-tRNA synthetase (MetRS). In complex cellular mixtures, proteins made in cells that express the mutant synthetase can be tagged with affinity reagents (for detection or enrichment) or fluorescent dyes (for imaging). Proteins made in cells that do not express the mutant synthetase are neither labeled nor detected.</description><subject>Affinity Labels - metabolism</subject><subject>Alanine - analogs & derivatives</subject><subject>Alanine - metabolism</subject><subject>Amino Acyl-tRNA Synthetases - genetics</subject><subject>Amino Acyl-tRNA Synthetases - metabolism</subject><subject>Animals</subject><subject>Biochemical Engineering</subject><subject>Biochemistry</subject><subject>Bioorganic Chemistry</subject><subject>brief-communication</subject><subject>Cell Biology</subject><subject>Cell Line</subject><subject>Cellular biology</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Chemistry/Food Science</subject><subject>Coculture Techniques</subject><subject>Dyes</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Escherichia coli - genetics</subject><subject>Escherichia coli - metabolism</subject><subject>Macrophages, Alveolar - 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| ispartof | Nature chemical biology, 2009-10, Vol.5 (10), p.715-717 |
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| source | MEDLINE; Nature; SpringerLink Journals - AutoHoldings |
| subjects | Affinity Labels - metabolism Alanine - analogs & derivatives Alanine - metabolism Amino Acyl-tRNA Synthetases - genetics Amino Acyl-tRNA Synthetases - metabolism Animals Biochemical Engineering Biochemistry Bioorganic Chemistry brief-communication Cell Biology Cell Line Cellular biology Chemistry Chemistry and Materials Science Chemistry/Food Science Coculture Techniques Dyes Electrophoresis, Polyacrylamide Gel Escherichia coli - genetics Escherichia coli - metabolism Macrophages, Alveolar - metabolism Macrophages, Alveolar - microbiology Metabolism Methionine-tRNA Ligase - genetics Methionine-tRNA Ligase - metabolism Mice Mutagenesis, Site-Directed Mutants Mutation Norleucine - analogs & derivatives Norleucine - metabolism Protein Biosynthesis Proteins Proteins - metabolism Reagents |
| title | Cell-selective metabolic labeling of proteins |
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