HIV-1 sexual transmission: early events of HIV-1 infection of human cervico-vaginal tissue in an optimized ex vivo model
Infection and dissemination of human immunodeficiency virus (HIV)-1 through the female body after vaginal intercourse depends on the activation/differentiation status of mucosal CD4 T cells. In this study, we investigated this status and the susceptibility to HIV-1 infection of human cervico-vaginal...
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| Veröffentlicht in: | Mucosal immunology 2010-05, Vol.3 (3), p.280-290 |
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| creator | Saba, E Grivel, J-C Vanpouille, C Brichacek, B Fitzgerald, W Margolis, L Lisco, A |
| description | Infection and dissemination of human immunodeficiency virus (HIV)-1 through the female body after vaginal intercourse depends on the activation/differentiation status of mucosal CD4 T cells. In this study, we investigated this status and the susceptibility to HIV-1 infection of human cervico-vaginal tissue
ex vivo
. We found that virtually all T cells are of the effector memory phenotype with broad CC chemokine receptor 5 (CCR5) expression. As it does
in vivo
, human cervico-vaginal tissue
ex vivo
preferentially supports the productive infection of R5 HIV-1 rather than that of X4 HIV-1 in spite of the broad expression of CXC chemokine receptor 4 (CXCR4). X4 HIV-1 replicated only in the few tissues that were enriched in CD27
+
CD28
+
effector memory CD4 T cells. Productive infection of R5 HIV-1 occurred preferentially in activated CD38
+
CD4 T cells and was followed by a similar activation of HIV-1-uninfected (bystander) CD4 T cells that may amplify viral infection. These results provide new insights into the dependence of HIV-1 infection and dissemination on the activation/differentiation of cervico-vaginal lymphocytes. |
| doi_str_mv | 10.1038/mi.2010.2 |
| format | Article |
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ex vivo
. We found that virtually all T cells are of the effector memory phenotype with broad CC chemokine receptor 5 (CCR5) expression. As it does
in vivo
, human cervico-vaginal tissue
ex vivo
preferentially supports the productive infection of R5 HIV-1 rather than that of X4 HIV-1 in spite of the broad expression of CXC chemokine receptor 4 (CXCR4). X4 HIV-1 replicated only in the few tissues that were enriched in CD27
+
CD28
+
effector memory CD4 T cells. Productive infection of R5 HIV-1 occurred preferentially in activated CD38
+
CD4 T cells and was followed by a similar activation of HIV-1-uninfected (bystander) CD4 T cells that may amplify viral infection. These results provide new insights into the dependence of HIV-1 infection and dissemination on the activation/differentiation of cervico-vaginal lymphocytes.</description><identifier>ISSN: 1933-0219</identifier><identifier>EISSN: 1935-3456</identifier><identifier>DOI: 10.1038/mi.2010.2</identifier><identifier>PMID: 20147895</identifier><language>eng</language><publisher>New York: Nature Publishing Group US</publisher><subject>631/326/596/2563 ; 692/698/1543/1565/1597/554/1898 ; 692/699/255/1901 ; ADP-ribosyl Cyclase 1 - immunology ; ADP-ribosyl Cyclase 1 - metabolism ; Allergology ; Antibodies ; Biomedical and Life Sciences ; Biomedicine ; Bystander Effect - immunology ; CD4-Positive T-Lymphocytes - immunology ; CD4-Positive T-Lymphocytes - metabolism ; CD4-Positive T-Lymphocytes - virology ; Cervix Uteri ; Female ; Gastroenterology ; HIV Infections - immunology ; HIV Infections - metabolism ; HIV Infections - transmission ; HIV-1 - immunology ; HIV-1 - metabolism ; Humans ; Immunology ; Male ; Membrane Glycoproteins - immunology ; Membrane Glycoproteins - metabolism ; Receptors, CCR5 - immunology ; Receptors, CCR5 - metabolism ; Receptors, CXCR4 - immunology ; Receptors, CXCR4 - metabolism ; Tissue Culture Techniques ; Vagina ; Virus Replication - immunology</subject><ispartof>Mucosal immunology, 2010-05, Vol.3 (3), p.280-290</ispartof><rights>Society for Mucosal Immunology 2010</rights><rights>Copyright Nature Publishing Group May 2010</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c503t-97a434a0e55a8aaca750110629eed2a98d91b234552fa761de4702d0d4ac4e923</citedby><cites>FETCH-LOGICAL-c503t-97a434a0e55a8aaca750110629eed2a98d91b234552fa761de4702d0d4ac4e923</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.proquest.com/docview/1782998412?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>230,314,780,784,885,27922,27923,64383,64385,64387,72239</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20147895$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Saba, E</creatorcontrib><creatorcontrib>Grivel, J-C</creatorcontrib><creatorcontrib>Vanpouille, C</creatorcontrib><creatorcontrib>Brichacek, B</creatorcontrib><creatorcontrib>Fitzgerald, W</creatorcontrib><creatorcontrib>Margolis, L</creatorcontrib><creatorcontrib>Lisco, A</creatorcontrib><title>HIV-1 sexual transmission: early events of HIV-1 infection of human cervico-vaginal tissue in an optimized ex vivo model</title><title>Mucosal immunology</title><addtitle>Mucosal Immunol</addtitle><addtitle>Mucosal Immunol</addtitle><description>Infection and dissemination of human immunodeficiency virus (HIV)-1 through the female body after vaginal intercourse depends on the activation/differentiation status of mucosal CD4 T cells. In this study, we investigated this status and the susceptibility to HIV-1 infection of human cervico-vaginal tissue
ex vivo
. We found that virtually all T cells are of the effector memory phenotype with broad CC chemokine receptor 5 (CCR5) expression. As it does
in vivo
, human cervico-vaginal tissue
ex vivo
preferentially supports the productive infection of R5 HIV-1 rather than that of X4 HIV-1 in spite of the broad expression of CXC chemokine receptor 4 (CXCR4). X4 HIV-1 replicated only in the few tissues that were enriched in CD27
+
CD28
+
effector memory CD4 T cells. Productive infection of R5 HIV-1 occurred preferentially in activated CD38
+
CD4 T cells and was followed by a similar activation of HIV-1-uninfected (bystander) CD4 T cells that may amplify viral infection. These results provide new insights into the dependence of HIV-1 infection and dissemination on the activation/differentiation of cervico-vaginal lymphocytes.</description><subject>631/326/596/2563</subject><subject>692/698/1543/1565/1597/554/1898</subject><subject>692/699/255/1901</subject><subject>ADP-ribosyl Cyclase 1 - immunology</subject><subject>ADP-ribosyl Cyclase 1 - metabolism</subject><subject>Allergology</subject><subject>Antibodies</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Bystander Effect - immunology</subject><subject>CD4-Positive T-Lymphocytes - immunology</subject><subject>CD4-Positive T-Lymphocytes - metabolism</subject><subject>CD4-Positive T-Lymphocytes - virology</subject><subject>Cervix Uteri</subject><subject>Female</subject><subject>Gastroenterology</subject><subject>HIV Infections - immunology</subject><subject>HIV Infections - metabolism</subject><subject>HIV Infections - transmission</subject><subject>HIV-1 - 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immunology</topic><topic>ADP-ribosyl Cyclase 1 - metabolism</topic><topic>Allergology</topic><topic>Antibodies</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Bystander Effect - immunology</topic><topic>CD4-Positive T-Lymphocytes - immunology</topic><topic>CD4-Positive T-Lymphocytes - metabolism</topic><topic>CD4-Positive T-Lymphocytes - virology</topic><topic>Cervix Uteri</topic><topic>Female</topic><topic>Gastroenterology</topic><topic>HIV Infections - immunology</topic><topic>HIV Infections - metabolism</topic><topic>HIV Infections - transmission</topic><topic>HIV-1 - immunology</topic><topic>HIV-1 - metabolism</topic><topic>Humans</topic><topic>Immunology</topic><topic>Male</topic><topic>Membrane Glycoproteins - immunology</topic><topic>Membrane Glycoproteins - metabolism</topic><topic>Receptors, CCR5 - immunology</topic><topic>Receptors, CCR5 - metabolism</topic><topic>Receptors, CXCR4 - immunology</topic><topic>Receptors, CXCR4 - metabolism</topic><topic>Tissue Culture Techniques</topic><topic>Vagina</topic><topic>Virus Replication - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Mucosal immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Saba, E</au><au>Grivel, J-C</au><au>Vanpouille, C</au><au>Brichacek, B</au><au>Fitzgerald, W</au><au>Margolis, L</au><au>Lisco, A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>HIV-1 sexual transmission: early events of HIV-1 infection of human cervico-vaginal tissue in an optimized ex vivo model</atitle><jtitle>Mucosal immunology</jtitle><stitle>Mucosal Immunol</stitle><addtitle>Mucosal Immunol</addtitle><date>2010-05-01</date><risdate>2010</risdate><volume>3</volume><issue>3</issue><spage>280</spage><epage>290</epage><pages>280-290</pages><issn>1933-0219</issn><eissn>1935-3456</eissn><abstract>Infection and dissemination of human immunodeficiency virus (HIV)-1 through the female body after vaginal intercourse depends on the activation/differentiation status of mucosal CD4 T cells. In this study, we investigated this status and the susceptibility to HIV-1 infection of human cervico-vaginal tissue
ex vivo
. We found that virtually all T cells are of the effector memory phenotype with broad CC chemokine receptor 5 (CCR5) expression. As it does
in vivo
, human cervico-vaginal tissue
ex vivo
preferentially supports the productive infection of R5 HIV-1 rather than that of X4 HIV-1 in spite of the broad expression of CXC chemokine receptor 4 (CXCR4). X4 HIV-1 replicated only in the few tissues that were enriched in CD27
+
CD28
+
effector memory CD4 T cells. Productive infection of R5 HIV-1 occurred preferentially in activated CD38
+
CD4 T cells and was followed by a similar activation of HIV-1-uninfected (bystander) CD4 T cells that may amplify viral infection. These results provide new insights into the dependence of HIV-1 infection and dissemination on the activation/differentiation of cervico-vaginal lymphocytes.</abstract><cop>New York</cop><pub>Nature Publishing Group US</pub><pmid>20147895</pmid><doi>10.1038/mi.2010.2</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Mucosal immunology, 2010-05, Vol.3 (3), p.280-290 |
| issn | 1933-0219 1935-3456 |
| language | eng |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; ProQuest Central UK/Ireland; Alma/SFX Local Collection |
| subjects | 631/326/596/2563 692/698/1543/1565/1597/554/1898 692/699/255/1901 ADP-ribosyl Cyclase 1 - immunology ADP-ribosyl Cyclase 1 - metabolism Allergology Antibodies Biomedical and Life Sciences Biomedicine Bystander Effect - immunology CD4-Positive T-Lymphocytes - immunology CD4-Positive T-Lymphocytes - metabolism CD4-Positive T-Lymphocytes - virology Cervix Uteri Female Gastroenterology HIV Infections - immunology HIV Infections - metabolism HIV Infections - transmission HIV-1 - immunology HIV-1 - metabolism Humans Immunology Male Membrane Glycoproteins - immunology Membrane Glycoproteins - metabolism Receptors, CCR5 - immunology Receptors, CCR5 - metabolism Receptors, CXCR4 - immunology Receptors, CXCR4 - metabolism Tissue Culture Techniques Vagina Virus Replication - immunology |
| title | HIV-1 sexual transmission: early events of HIV-1 infection of human cervico-vaginal tissue in an optimized ex vivo model |
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