TFIID-specific yeast TAF40 is essential for the majority of RNA polymerase II-mediated transcription in vivo
Many questions remain concerning the role of TFIID TBP-associated factors (TAFs) in transcription, including whether TAFs are required at most or only a small subset of promoters. It was shown previously that three histone-like TAFs are broadly required for transcription, but interpretation of this...
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| Veröffentlicht in: | Genes & development 1999-10, Vol.13 (19), p.2484-2489 |
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| creator | Komarnitsky, P B Michel, B Buratowski, S |
| description | Many questions remain concerning the role of TFIID TBP-associated factors (TAFs) in transcription, including whether TAFs are required at most or only a small subset of promoters. It was shown previously that three histone-like TAFs are broadly required for transcription, but interpretation of this observation is complicated because these proteins are components of both TFIID and the SAGA histone acetyltransferase complex. Here we show that mutations in the yeast TFIID-specific protein Taf40 lead to a general cessation of transcription, even in the presence of excess TBP, suggesting that the TFIID complex is required at most promoters in vivo. |
| doi_str_mv | 10.1101/gad.13.19.2484 |
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It was shown previously that three histone-like TAFs are broadly required for transcription, but interpretation of this observation is complicated because these proteins are components of both TFIID and the SAGA histone acetyltransferase complex. Here we show that mutations in the yeast TFIID-specific protein Taf40 lead to a general cessation of transcription, even in the presence of excess TBP, suggesting that the TFIID complex is required at most promoters in vivo.</description><identifier>ISSN: 0890-9369</identifier><identifier>DOI: 10.1101/gad.13.19.2484</identifier><identifier>PMID: 10521393</identifier><language>eng</language><publisher>United States: Cold Spring Harbor Laboratory Press</publisher><subject>DNA-Binding Proteins - genetics ; DNA-Binding Proteins - metabolism ; Gene Expression ; Mutagenesis ; Research Communication ; RNA Polymerase II - metabolism ; Saccharomyces cerevisiae Proteins ; TATA-Binding Protein Associated Factors ; TATA-Box Binding Protein ; Temperature ; Transcription Factor TFIID ; Transcription Factors - genetics ; Transcription Factors - metabolism ; Transcription Factors, TFII - metabolism ; Transcription, Genetic ; Yeasts - genetics</subject><ispartof>Genes & development, 1999-10, Vol.13 (19), p.2484-2489</ispartof><rights>Copyright © 1999, Cold Spring Harbor Laboratory Press 1999</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c482t-f9441776883daa8d3349027c5a317461326317a20af9e82c6bb542eb279ca1ae3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC317065/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC317065/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10521393$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Komarnitsky, P B</creatorcontrib><creatorcontrib>Michel, B</creatorcontrib><creatorcontrib>Buratowski, S</creatorcontrib><title>TFIID-specific yeast TAF40 is essential for the majority of RNA polymerase II-mediated transcription in vivo</title><title>Genes & development</title><addtitle>Genes Dev</addtitle><description>Many questions remain concerning the role of TFIID TBP-associated factors (TAFs) in transcription, including whether TAFs are required at most or only a small subset of promoters. 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Here we show that mutations in the yeast TFIID-specific protein Taf40 lead to a general cessation of transcription, even in the presence of excess TBP, suggesting that the TFIID complex is required at most promoters in vivo.</description><subject>DNA-Binding Proteins - genetics</subject><subject>DNA-Binding Proteins - metabolism</subject><subject>Gene Expression</subject><subject>Mutagenesis</subject><subject>Research Communication</subject><subject>RNA Polymerase II - metabolism</subject><subject>Saccharomyces cerevisiae Proteins</subject><subject>TATA-Binding Protein Associated Factors</subject><subject>TATA-Box Binding Protein</subject><subject>Temperature</subject><subject>Transcription Factor TFIID</subject><subject>Transcription Factors - genetics</subject><subject>Transcription Factors - metabolism</subject><subject>Transcription Factors, TFII - metabolism</subject><subject>Transcription, Genetic</subject><subject>Yeasts - genetics</subject><issn>0890-9369</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUFv1DAQhX0A0VK4ckQ-cUvqsR0nPnBYFZZGqkBCy9madZzWVRIH27vS_ntcbYXKidOMNN8bvadHyAdgNQCD63scahA16JrLTr4il6zTrNJC6QvyNqVHxphiSr0hF8AaDkKLSzLttn3_pUqrs370lp4cpkx3m61k1CfqUnJL9jjRMUSaHxyd8TFEn080jPTn9w1dw3SaXcTkaN9Xsxs8ZjfQHHFJNvo1-7BQv9CjP4Z35PWIU3Lvn-cV-bX9uru5re5-fOtvNneVlR3P1ailhLZVXScGxG4QQmrGW9uggFYqEFyVBTnDUbuOW7XfN5K7PW-1RUAnrsjn89_1sC-ObIkQcTJr9DPGkwnozb-XxT-Y-3A05S1TTdF_etbH8PvgUjazT9ZNEy4uHJJpWVdMsP-D0IoGJNMFrM-gjSGl6Ma_ZoCZp_JMKc-AMKDNU3lF8PFlhBf4uTnxB9mjl9E</recordid><startdate>19991001</startdate><enddate>19991001</enddate><creator>Komarnitsky, P B</creator><creator>Michel, B</creator><creator>Buratowski, S</creator><general>Cold Spring Harbor Laboratory Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19991001</creationdate><title>TFIID-specific yeast TAF40 is essential for the majority of RNA polymerase II-mediated transcription in vivo</title><author>Komarnitsky, P B ; Michel, B ; Buratowski, S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c482t-f9441776883daa8d3349027c5a317461326317a20af9e82c6bb542eb279ca1ae3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>DNA-Binding Proteins - genetics</topic><topic>DNA-Binding Proteins - metabolism</topic><topic>Gene Expression</topic><topic>Mutagenesis</topic><topic>Research Communication</topic><topic>RNA Polymerase II - metabolism</topic><topic>Saccharomyces cerevisiae Proteins</topic><topic>TATA-Binding Protein Associated Factors</topic><topic>TATA-Box Binding Protein</topic><topic>Temperature</topic><topic>Transcription Factor TFIID</topic><topic>Transcription Factors - genetics</topic><topic>Transcription Factors - metabolism</topic><topic>Transcription Factors, TFII - metabolism</topic><topic>Transcription, Genetic</topic><topic>Yeasts - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Komarnitsky, P B</creatorcontrib><creatorcontrib>Michel, B</creatorcontrib><creatorcontrib>Buratowski, S</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Genes & development</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Komarnitsky, P B</au><au>Michel, B</au><au>Buratowski, S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>TFIID-specific yeast TAF40 is essential for the majority of RNA polymerase II-mediated transcription in vivo</atitle><jtitle>Genes & development</jtitle><addtitle>Genes Dev</addtitle><date>1999-10-01</date><risdate>1999</risdate><volume>13</volume><issue>19</issue><spage>2484</spage><epage>2489</epage><pages>2484-2489</pages><issn>0890-9369</issn><abstract>Many questions remain concerning the role of TFIID TBP-associated factors (TAFs) in transcription, including whether TAFs are required at most or only a small subset of promoters. 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| ispartof | Genes & development, 1999-10, Vol.13 (19), p.2484-2489 |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | DNA-Binding Proteins - genetics DNA-Binding Proteins - metabolism Gene Expression Mutagenesis Research Communication RNA Polymerase II - metabolism Saccharomyces cerevisiae Proteins TATA-Binding Protein Associated Factors TATA-Box Binding Protein Temperature Transcription Factor TFIID Transcription Factors - genetics Transcription Factors - metabolism Transcription Factors, TFII - metabolism Transcription, Genetic Yeasts - genetics |
| title | TFIID-specific yeast TAF40 is essential for the majority of RNA polymerase II-mediated transcription in vivo |
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