Pulmonary pathology of pandemic influenza A/H1N1 virus (2009)-infected ferrets upon longitudinal evaluation by computed tomography
We investigated the development of pulmonary lesions in ferrets by means of computed tomography (CT) following infection with the 2009 pandemic A/H1N1 influenza virus and compared the scans with gross pathology, histopathology and immunohistochemistry. Ground-glass opacities observed by CT scanning...
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| Veröffentlicht in: | Journal of general virology 2011-08, Vol.92 (Pt 8), p.1854-1858 |
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| creator | VELDHUIS KROEZE, Edwin J. B AMERONGEN, Geert Van DIJKSHOORN, Marcel L SIMON, James H DE WAAL, Leon HARTMANN, Leneke J. C KRESTIN, Gabriel P KUIKEN, Thijs OSTERHAUS, Albert D. M. E STITTELAAR, Koert J |
| description | We investigated the development of pulmonary lesions in ferrets by means of computed tomography (CT) following infection with the 2009 pandemic A/H1N1 influenza virus and compared the scans with gross pathology, histopathology and immunohistochemistry. Ground-glass opacities observed by CT scanning in all infected lungs corresponded to areas of alveolar oedema at necropsy. These areas were most pronounced on day 3 and gradually decreased from days 4 to 7 post-infection. This pilot study shows that the non-invasive imaging procedure allows quantification and characterization of influenza-induced pulmonary lesions in living animals under biosafety level 3 conditions and can thus be used in pre-clinical pharmaceutical efficacy studies. |
| doi_str_mv | 10.1099/vir.0.032805-0 |
| format | Article |
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B ; AMERONGEN, Geert Van ; DIJKSHOORN, Marcel L ; SIMON, James H ; DE WAAL, Leon ; HARTMANN, Leneke J. C ; KRESTIN, Gabriel P ; KUIKEN, Thijs ; OSTERHAUS, Albert D. M. E ; STITTELAAR, Koert J</creator><creatorcontrib>VELDHUIS KROEZE, Edwin J. B ; AMERONGEN, Geert Van ; DIJKSHOORN, Marcel L ; SIMON, James H ; DE WAAL, Leon ; HARTMANN, Leneke J. C ; KRESTIN, Gabriel P ; KUIKEN, Thijs ; OSTERHAUS, Albert D. M. E ; STITTELAAR, Koert J</creatorcontrib><description>We investigated the development of pulmonary lesions in ferrets by means of computed tomography (CT) following infection with the 2009 pandemic A/H1N1 influenza virus and compared the scans with gross pathology, histopathology and immunohistochemistry. Ground-glass opacities observed by CT scanning in all infected lungs corresponded to areas of alveolar oedema at necropsy. These areas were most pronounced on day 3 and gradually decreased from days 4 to 7 post-infection. This pilot study shows that the non-invasive imaging procedure allows quantification and characterization of influenza-induced pulmonary lesions in living animals under biosafety level 3 conditions and can thus be used in pre-clinical pharmaceutical efficacy studies.</description><identifier>ISSN: 0022-1317</identifier><identifier>EISSN: 1465-2099</identifier><identifier>DOI: 10.1099/vir.0.032805-0</identifier><identifier>PMID: 21543558</identifier><identifier>CODEN: JGVIAY</identifier><language>eng</language><publisher>Reading: Society for General Microbiology</publisher><subject>Animal ; Animals ; Biological and medical sciences ; Disease Models, Animal ; Female ; Ferrets ; Fundamental and applied biological sciences. Psychology ; Humans ; Influenza A Virus, H1N1 Subtype - physiology ; Influenza, Human - diagnostic imaging ; Influenza, Human - epidemiology ; Influenza, Human - pathology ; Influenza, Human - virology ; Lung - pathology ; Lung - virology ; Microbiology ; Miscellaneous ; Pandemics ; Pathology - methods ; Short Communication ; Tomography, X-Ray Computed - methods ; Virology</subject><ispartof>Journal of general virology, 2011-08, Vol.92 (Pt 8), p.1854-1858</ispartof><rights>2015 INIST-CNRS</rights><rights>2011 SGM</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c419t-600c9fad48e88085d75025966670636c8667555984dbdc87badeaa869bdb9633</citedby><cites>FETCH-LOGICAL-c419t-600c9fad48e88085d75025966670636c8667555984dbdc87badeaa869bdb9633</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,3746,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=24349749$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21543558$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>VELDHUIS KROEZE, Edwin J. B</creatorcontrib><creatorcontrib>AMERONGEN, Geert Van</creatorcontrib><creatorcontrib>DIJKSHOORN, Marcel L</creatorcontrib><creatorcontrib>SIMON, James H</creatorcontrib><creatorcontrib>DE WAAL, Leon</creatorcontrib><creatorcontrib>HARTMANN, Leneke J. C</creatorcontrib><creatorcontrib>KRESTIN, Gabriel P</creatorcontrib><creatorcontrib>KUIKEN, Thijs</creatorcontrib><creatorcontrib>OSTERHAUS, Albert D. M. E</creatorcontrib><creatorcontrib>STITTELAAR, Koert J</creatorcontrib><title>Pulmonary pathology of pandemic influenza A/H1N1 virus (2009)-infected ferrets upon longitudinal evaluation by computed tomography</title><title>Journal of general virology</title><addtitle>J Gen Virol</addtitle><description>We investigated the development of pulmonary lesions in ferrets by means of computed tomography (CT) following infection with the 2009 pandemic A/H1N1 influenza virus and compared the scans with gross pathology, histopathology and immunohistochemistry. Ground-glass opacities observed by CT scanning in all infected lungs corresponded to areas of alveolar oedema at necropsy. These areas were most pronounced on day 3 and gradually decreased from days 4 to 7 post-infection. This pilot study shows that the non-invasive imaging procedure allows quantification and characterization of influenza-induced pulmonary lesions in living animals under biosafety level 3 conditions and can thus be used in pre-clinical pharmaceutical efficacy studies.</description><subject>Animal</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Disease Models, Animal</subject><subject>Female</subject><subject>Ferrets</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Influenza A Virus, H1N1 Subtype - physiology</subject><subject>Influenza, Human - diagnostic imaging</subject><subject>Influenza, Human - epidemiology</subject><subject>Influenza, Human - pathology</subject><subject>Influenza, Human - virology</subject><subject>Lung - pathology</subject><subject>Lung - virology</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>Pandemics</subject><subject>Pathology - methods</subject><subject>Short Communication</subject><subject>Tomography, X-Ray Computed - methods</subject><subject>Virology</subject><issn>0022-1317</issn><issn>1465-2099</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkcFvFCEUxonR2HX16tFwMephtjADDFxMmkatSdN66J0wwOxiGBhh2GQ9-pdLs2ttTw_4fnwP3gfAW4w2GAlxvndpgzaoazmiDXoGVpgw2rRVeg5WCLVtgzvcn4FXOf9ECBNC-5fgrMWUdJTyFfjzo_gpBpUOcFbLLvq4PcA41k0wdnIaujD6YsNvBS_Or_ANhrVjyfBji5D41FTV6sUaONqU7JJhmWOAPoatW4pxQXlo98oXtbh6PhygjtNc7i8scYrbpObd4TV4MSqf7ZtTXYO7r1_uLq-a69tv3y8vrhtNsFgahpAWozKEW84Rp6anqKWCMdYj1jHN64JSKjgxg9G8H5SxSnEmBjMI1nVr8PloO5dhskbbsCTl5ZzcVH8vo3LyqRLcTm7jXnaY9Zy31eDDySDFX8XmRU4ua-u9CjaWLHnfEyTqYCu5OZI6xZyTHR-6YCTvY5N1iBLJY2y1rMG7x297wP_lVIH3J0BlrfyYVNAu_-dIR0RPRPcXF-yjLg</recordid><startdate>20110801</startdate><enddate>20110801</enddate><creator>VELDHUIS KROEZE, Edwin J. B</creator><creator>AMERONGEN, Geert Van</creator><creator>DIJKSHOORN, Marcel L</creator><creator>SIMON, James H</creator><creator>DE WAAL, Leon</creator><creator>HARTMANN, Leneke J. C</creator><creator>KRESTIN, Gabriel P</creator><creator>KUIKEN, Thijs</creator><creator>OSTERHAUS, Albert D. M. E</creator><creator>STITTELAAR, Koert J</creator><general>Society for General Microbiology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20110801</creationdate><title>Pulmonary pathology of pandemic influenza A/H1N1 virus (2009)-infected ferrets upon longitudinal evaluation by computed tomography</title><author>VELDHUIS KROEZE, Edwin J. B ; AMERONGEN, Geert Van ; DIJKSHOORN, Marcel L ; SIMON, James H ; DE WAAL, Leon ; HARTMANN, Leneke J. C ; KRESTIN, Gabriel P ; KUIKEN, Thijs ; OSTERHAUS, Albert D. 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Psychology</topic><topic>Humans</topic><topic>Influenza A Virus, H1N1 Subtype - physiology</topic><topic>Influenza, Human - diagnostic imaging</topic><topic>Influenza, Human - epidemiology</topic><topic>Influenza, Human - pathology</topic><topic>Influenza, Human - virology</topic><topic>Lung - pathology</topic><topic>Lung - virology</topic><topic>Microbiology</topic><topic>Miscellaneous</topic><topic>Pandemics</topic><topic>Pathology - methods</topic><topic>Short Communication</topic><topic>Tomography, X-Ray Computed - methods</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>VELDHUIS KROEZE, Edwin J. B</creatorcontrib><creatorcontrib>AMERONGEN, Geert Van</creatorcontrib><creatorcontrib>DIJKSHOORN, Marcel L</creatorcontrib><creatorcontrib>SIMON, James H</creatorcontrib><creatorcontrib>DE WAAL, Leon</creatorcontrib><creatorcontrib>HARTMANN, Leneke J. 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C</au><au>KRESTIN, Gabriel P</au><au>KUIKEN, Thijs</au><au>OSTERHAUS, Albert D. M. E</au><au>STITTELAAR, Koert J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Pulmonary pathology of pandemic influenza A/H1N1 virus (2009)-infected ferrets upon longitudinal evaluation by computed tomography</atitle><jtitle>Journal of general virology</jtitle><addtitle>J Gen Virol</addtitle><date>2011-08-01</date><risdate>2011</risdate><volume>92</volume><issue>Pt 8</issue><spage>1854</spage><epage>1858</epage><pages>1854-1858</pages><issn>0022-1317</issn><eissn>1465-2099</eissn><coden>JGVIAY</coden><abstract>We investigated the development of pulmonary lesions in ferrets by means of computed tomography (CT) following infection with the 2009 pandemic A/H1N1 influenza virus and compared the scans with gross pathology, histopathology and immunohistochemistry. Ground-glass opacities observed by CT scanning in all infected lungs corresponded to areas of alveolar oedema at necropsy. These areas were most pronounced on day 3 and gradually decreased from days 4 to 7 post-infection. This pilot study shows that the non-invasive imaging procedure allows quantification and characterization of influenza-induced pulmonary lesions in living animals under biosafety level 3 conditions and can thus be used in pre-clinical pharmaceutical efficacy studies.</abstract><cop>Reading</cop><pub>Society for General Microbiology</pub><pmid>21543558</pmid><doi>10.1099/vir.0.032805-0</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animal Animals Biological and medical sciences Disease Models, Animal Female Ferrets Fundamental and applied biological sciences. Psychology Humans Influenza A Virus, H1N1 Subtype - physiology Influenza, Human - diagnostic imaging Influenza, Human - epidemiology Influenza, Human - pathology Influenza, Human - virology Lung - pathology Lung - virology Microbiology Miscellaneous Pandemics Pathology - methods Short Communication Tomography, X-Ray Computed - methods Virology |
| title | Pulmonary pathology of pandemic influenza A/H1N1 virus (2009)-infected ferrets upon longitudinal evaluation by computed tomography |
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