Human telomerase contains evolutionarily conserved catalytic and structural subunits
We have cloned and characterized a human gene encoding TP2 (telomerase-associated protein 2), a protein with similarity to reverse transcriptases and the catalytic telomerase subunits from Saccharomyces cerevisiae and Euplotes aediculatus. Indirect immunofluorescence revealed that TP2 was localized...
Gespeichert in:
| Veröffentlicht in: | Genes & development 1997-12, Vol.11 (23), p.3109-3115 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 3115 |
|---|---|
| container_issue | 23 |
| container_start_page | 3109 |
| container_title | Genes & development |
| container_volume | 11 |
| creator | Harrington, L Zhou, W McPhail, T Oulton, R Yeung, D S Mar, V Bass, M B Robinson, M O |
| description | We have cloned and characterized a human gene encoding TP2 (telomerase-associated protein 2), a protein with similarity to reverse transcriptases and the catalytic telomerase subunits from Saccharomyces cerevisiae and Euplotes aediculatus. Indirect immunofluorescence revealed that TP2 was localized to the nucleus. Using antibodies to endogenous and epitope-tagged TP2, we found that TP2 was associated specifically with human telomerase activity and the recently identified telomerase-associated protein TP1. Mutation of conserved residues within the reverse transcriptase domain of TP2 severely reduced associated telomerase activity. These results suggest that telomerase is an evolutionarily conserved multisubunit complex composed of both structural and catalytic subunits. |
| doi_str_mv | 10.1101/gad.11.23.3109 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_316744</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>16298785</sourcerecordid><originalsourceid>FETCH-LOGICAL-c481t-25961f00f7d0e6776cbe5f8d2aaac297d465e249d985d6e1d9e44579283332513</originalsourceid><addsrcrecordid>eNqFkbtPHDEQxq0IRI5HSxdpq3R7-O11kSJCSUBCooHa8tmzxGjXJn6cdP89e-KEQkU1o5nfN_pGH0KXBK8JweTqyfqlWVO2ZgTrL2hFBNe94EodoRUeNO41k_orOi3lGWMssZQn6ESzQUvOVujhps02dhWmNEO2BTqXYrUhlg62aWo1pGhzmHb7eYG8Bd85W-20q8F1Nvqu1NxcbdlOXWmbFkMt5-h4tFOBi0M9Q4-_fz1c3_R3939ur3_e9Y4PpPZUaElGjEflMUilpNuAGAdPrbWOauW5FEC59noQXgLxGjgXStOBMUYFYWfox9vdl7aZwTuIdbFhXnKYbd6ZZIP5uInhr3lKW8OIVJwv-u8HfU7_GpRq5lAcTJONkFoxSnOutaSfgkRSPahBLOD6DXQ5lZJhfDdDsNnnZZa8lsZQZvZ5LYJv_7_wjh8CYq_NZpPw</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>16298785</pqid></control><display><type>article</type><title>Human telomerase contains evolutionarily conserved catalytic and structural subunits</title><source>MEDLINE</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><creator>Harrington, L ; Zhou, W ; McPhail, T ; Oulton, R ; Yeung, D S ; Mar, V ; Bass, M B ; Robinson, M O</creator><creatorcontrib>Harrington, L ; Zhou, W ; McPhail, T ; Oulton, R ; Yeung, D S ; Mar, V ; Bass, M B ; Robinson, M O</creatorcontrib><description>We have cloned and characterized a human gene encoding TP2 (telomerase-associated protein 2), a protein with similarity to reverse transcriptases and the catalytic telomerase subunits from Saccharomyces cerevisiae and Euplotes aediculatus. Indirect immunofluorescence revealed that TP2 was localized to the nucleus. Using antibodies to endogenous and epitope-tagged TP2, we found that TP2 was associated specifically with human telomerase activity and the recently identified telomerase-associated protein TP1. Mutation of conserved residues within the reverse transcriptase domain of TP2 severely reduced associated telomerase activity. These results suggest that telomerase is an evolutionarily conserved multisubunit complex composed of both structural and catalytic subunits.</description><identifier>ISSN: 0890-9369</identifier><identifier>EISSN: 1549-5477</identifier><identifier>DOI: 10.1101/gad.11.23.3109</identifier><identifier>PMID: 9389643</identifier><language>eng</language><publisher>United States: Cold Spring Harbor Laboratory Press</publisher><subject>Amino Acid Sequence ; Binding Sites ; Carrier Proteins - genetics ; Carrier Proteins - metabolism ; Catalysis ; Cell Nucleus - metabolism ; Conserved Sequence ; DNA-Binding Proteins ; Evolution, Molecular ; HeLa Cells ; Humans ; Molecular Sequence Data ; Proteins - genetics ; Proteins - metabolism ; Research Communication ; RNA ; RNA-Directed DNA Polymerase - genetics ; Sequence Homology, Amino Acid ; Space life sciences ; Telomerase - metabolism ; Tumor Cells, Cultured</subject><ispartof>Genes & development, 1997-12, Vol.11 (23), p.3109-3115</ispartof><rights>Copyright © 1997, Cold Spring Harbor Laboratory Press 1997</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c481t-25961f00f7d0e6776cbe5f8d2aaac297d465e249d985d6e1d9e44579283332513</citedby><cites>FETCH-LOGICAL-c481t-25961f00f7d0e6776cbe5f8d2aaac297d465e249d985d6e1d9e44579283332513</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC316744/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC316744/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9389643$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Harrington, L</creatorcontrib><creatorcontrib>Zhou, W</creatorcontrib><creatorcontrib>McPhail, T</creatorcontrib><creatorcontrib>Oulton, R</creatorcontrib><creatorcontrib>Yeung, D S</creatorcontrib><creatorcontrib>Mar, V</creatorcontrib><creatorcontrib>Bass, M B</creatorcontrib><creatorcontrib>Robinson, M O</creatorcontrib><title>Human telomerase contains evolutionarily conserved catalytic and structural subunits</title><title>Genes & development</title><addtitle>Genes Dev</addtitle><description>We have cloned and characterized a human gene encoding TP2 (telomerase-associated protein 2), a protein with similarity to reverse transcriptases and the catalytic telomerase subunits from Saccharomyces cerevisiae and Euplotes aediculatus. Indirect immunofluorescence revealed that TP2 was localized to the nucleus. Using antibodies to endogenous and epitope-tagged TP2, we found that TP2 was associated specifically with human telomerase activity and the recently identified telomerase-associated protein TP1. Mutation of conserved residues within the reverse transcriptase domain of TP2 severely reduced associated telomerase activity. These results suggest that telomerase is an evolutionarily conserved multisubunit complex composed of both structural and catalytic subunits.</description><subject>Amino Acid Sequence</subject><subject>Binding Sites</subject><subject>Carrier Proteins - genetics</subject><subject>Carrier Proteins - metabolism</subject><subject>Catalysis</subject><subject>Cell Nucleus - metabolism</subject><subject>Conserved Sequence</subject><subject>DNA-Binding Proteins</subject><subject>Evolution, Molecular</subject><subject>HeLa Cells</subject><subject>Humans</subject><subject>Molecular Sequence Data</subject><subject>Proteins - genetics</subject><subject>Proteins - metabolism</subject><subject>Research Communication</subject><subject>RNA</subject><subject>RNA-Directed DNA Polymerase - genetics</subject><subject>Sequence Homology, Amino Acid</subject><subject>Space life sciences</subject><subject>Telomerase - metabolism</subject><subject>Tumor Cells, Cultured</subject><issn>0890-9369</issn><issn>1549-5477</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkbtPHDEQxq0IRI5HSxdpq3R7-O11kSJCSUBCooHa8tmzxGjXJn6cdP89e-KEQkU1o5nfN_pGH0KXBK8JweTqyfqlWVO2ZgTrL2hFBNe94EodoRUeNO41k_orOi3lGWMssZQn6ESzQUvOVujhps02dhWmNEO2BTqXYrUhlg62aWo1pGhzmHb7eYG8Bd85W-20q8F1Nvqu1NxcbdlOXWmbFkMt5-h4tFOBi0M9Q4-_fz1c3_R3939ur3_e9Y4PpPZUaElGjEflMUilpNuAGAdPrbWOauW5FEC59noQXgLxGjgXStOBMUYFYWfox9vdl7aZwTuIdbFhXnKYbd6ZZIP5uInhr3lKW8OIVJwv-u8HfU7_GpRq5lAcTJONkFoxSnOutaSfgkRSPahBLOD6DXQ5lZJhfDdDsNnnZZa8lsZQZvZ5LYJv_7_wjh8CYq_NZpPw</recordid><startdate>19971201</startdate><enddate>19971201</enddate><creator>Harrington, L</creator><creator>Zhou, W</creator><creator>McPhail, T</creator><creator>Oulton, R</creator><creator>Yeung, D S</creator><creator>Mar, V</creator><creator>Bass, M B</creator><creator>Robinson, M O</creator><general>Cold Spring Harbor Laboratory Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19971201</creationdate><title>Human telomerase contains evolutionarily conserved catalytic and structural subunits</title><author>Harrington, L ; Zhou, W ; McPhail, T ; Oulton, R ; Yeung, D S ; Mar, V ; Bass, M B ; Robinson, M O</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c481t-25961f00f7d0e6776cbe5f8d2aaac297d465e249d985d6e1d9e44579283332513</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Amino Acid Sequence</topic><topic>Binding Sites</topic><topic>Carrier Proteins - genetics</topic><topic>Carrier Proteins - metabolism</topic><topic>Catalysis</topic><topic>Cell Nucleus - metabolism</topic><topic>Conserved Sequence</topic><topic>DNA-Binding Proteins</topic><topic>Evolution, Molecular</topic><topic>HeLa Cells</topic><topic>Humans</topic><topic>Molecular Sequence Data</topic><topic>Proteins - genetics</topic><topic>Proteins - metabolism</topic><topic>Research Communication</topic><topic>RNA</topic><topic>RNA-Directed DNA Polymerase - genetics</topic><topic>Sequence Homology, Amino Acid</topic><topic>Space life sciences</topic><topic>Telomerase - metabolism</topic><topic>Tumor Cells, Cultured</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Harrington, L</creatorcontrib><creatorcontrib>Zhou, W</creatorcontrib><creatorcontrib>McPhail, T</creatorcontrib><creatorcontrib>Oulton, R</creatorcontrib><creatorcontrib>Yeung, D S</creatorcontrib><creatorcontrib>Mar, V</creatorcontrib><creatorcontrib>Bass, M B</creatorcontrib><creatorcontrib>Robinson, M O</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Genes & development</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Harrington, L</au><au>Zhou, W</au><au>McPhail, T</au><au>Oulton, R</au><au>Yeung, D S</au><au>Mar, V</au><au>Bass, M B</au><au>Robinson, M O</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Human telomerase contains evolutionarily conserved catalytic and structural subunits</atitle><jtitle>Genes & development</jtitle><addtitle>Genes Dev</addtitle><date>1997-12-01</date><risdate>1997</risdate><volume>11</volume><issue>23</issue><spage>3109</spage><epage>3115</epage><pages>3109-3115</pages><issn>0890-9369</issn><eissn>1549-5477</eissn><abstract>We have cloned and characterized a human gene encoding TP2 (telomerase-associated protein 2), a protein with similarity to reverse transcriptases and the catalytic telomerase subunits from Saccharomyces cerevisiae and Euplotes aediculatus. Indirect immunofluorescence revealed that TP2 was localized to the nucleus. Using antibodies to endogenous and epitope-tagged TP2, we found that TP2 was associated specifically with human telomerase activity and the recently identified telomerase-associated protein TP1. Mutation of conserved residues within the reverse transcriptase domain of TP2 severely reduced associated telomerase activity. These results suggest that telomerase is an evolutionarily conserved multisubunit complex composed of both structural and catalytic subunits.</abstract><cop>United States</cop><pub>Cold Spring Harbor Laboratory Press</pub><pmid>9389643</pmid><doi>10.1101/gad.11.23.3109</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0890-9369 |
| ispartof | Genes & development, 1997-12, Vol.11 (23), p.3109-3115 |
| issn | 0890-9369 1549-5477 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_316744 |
| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Amino Acid Sequence Binding Sites Carrier Proteins - genetics Carrier Proteins - metabolism Catalysis Cell Nucleus - metabolism Conserved Sequence DNA-Binding Proteins Evolution, Molecular HeLa Cells Humans Molecular Sequence Data Proteins - genetics Proteins - metabolism Research Communication RNA RNA-Directed DNA Polymerase - genetics Sequence Homology, Amino Acid Space life sciences Telomerase - metabolism Tumor Cells, Cultured |
| title | Human telomerase contains evolutionarily conserved catalytic and structural subunits |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-26T23%3A07%3A01IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Human%20telomerase%20contains%20evolutionarily%20conserved%20catalytic%20and%20structural%20subunits&rft.jtitle=Genes%20&%20development&rft.au=Harrington,%20L&rft.date=1997-12-01&rft.volume=11&rft.issue=23&rft.spage=3109&rft.epage=3115&rft.pages=3109-3115&rft.issn=0890-9369&rft.eissn=1549-5477&rft_id=info:doi/10.1101/gad.11.23.3109&rft_dat=%3Cproquest_pubme%3E16298785%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=16298785&rft_id=info:pmid/9389643&rfr_iscdi=true |