Drosophila SNS, a member of the immunoglobulin superfamily that is essential for myoblast fusion

The Drosophila sticks-and-stones (sns) locus was identified on the basis of its mutant phenotype, the complete absence of body wall muscles and corresponding presence of unfused myoblasts. The genetic location of the mutation responsible for this apparent defect in myoblast fusion was determined by...

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Veröffentlicht in:Genes & development 2000-06, Vol.14 (12), p.1498-1511
Hauptverfasser: Bour, B A, Chakravarti, M, West, J M, Abmayr, S M
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Chakravarti, M
West, J M
Abmayr, S M
description The Drosophila sticks-and-stones (sns) locus was identified on the basis of its mutant phenotype, the complete absence of body wall muscles and corresponding presence of unfused myoblasts. The genetic location of the mutation responsible for this apparent defect in myoblast fusion was determined by recombination and deficiency mapping, and the corresponding wild-type gene was isolated in a molecular walk. Identification of the SNS coding sequence revealed a putative member of the immunoglobulin superfamily (IgSF) of cell adhesion molecules. As anticipated from this homology, SNS is enriched at the membrane and clusters at discrete sites, coincident with the occurrence of myoblast fusion. Both the sns transcript and the encoded protein are expressed in precursors of the somatic and visceral musculature of the embryo. Within the presumptive somatic musculature, SNS expression is restricted to the putative fusion-competent cells and is not detected in unfused founder cells. Thus, SNS represents the first known marker for this subgroup of myoblasts, and provides an opportunity to identify pathways specifying this cell type as well as transcriptional regulators of fusion-specific genes. To these ends, we demonstrate that the presence of SNS-expressing cells is absolutely dependent on Notch, and that expression of SNS does not require the myogenic regulatory protein MEF2.
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The genetic location of the mutation responsible for this apparent defect in myoblast fusion was determined by recombination and deficiency mapping, and the corresponding wild-type gene was isolated in a molecular walk. Identification of the SNS coding sequence revealed a putative member of the immunoglobulin superfamily (IgSF) of cell adhesion molecules. As anticipated from this homology, SNS is enriched at the membrane and clusters at discrete sites, coincident with the occurrence of myoblast fusion. Both the sns transcript and the encoded protein are expressed in precursors of the somatic and visceral musculature of the embryo. Within the presumptive somatic musculature, SNS expression is restricted to the putative fusion-competent cells and is not detected in unfused founder cells. Thus, SNS represents the first known marker for this subgroup of myoblasts, and provides an opportunity to identify pathways specifying this cell type as well as transcriptional regulators of fusion-specific genes. To these ends, we demonstrate that the presence of SNS-expressing cells is absolutely dependent on Notch, and that expression of SNS does not require the myogenic regulatory protein MEF2.</abstract><cop>United States</cop><pub>Cold Spring Harbor Laboratory Press</pub><pmid>10859168</pmid><doi>10.1101/gad.14.12.1498</doi><tpages>14</tpages><oa>free_for_read</oa></addata></record>
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subjects Alleles
Amino Acid Sequence
Animals
Base Sequence
Cell Adhesion - genetics
Cell Membrane - metabolism
Chromosome Mapping
DNA-Binding Proteins - metabolism
Drosophila
Drosophila - embryology
Drosophila - genetics
Drosophila - immunology
Drosophila Proteins
Embryo, Nonmammalian - metabolism
Genes, Reporter - genetics
Immunoglobulins - biosynthesis
Immunoglobulins - chemistry
Immunoglobulins - genetics
Immunoglobulins - physiology
MEF2 Transcription Factors
Membrane Proteins - genetics
Membrane Proteins - metabolism
Models, Genetic
Molecular Sequence Data
Muscle Fibers, Skeletal - chemistry
Muscles - embryology
Mutation
Myogenic Regulatory Factors
Phenotype
Receptors, Notch
Research Paper
Sequence Homology, Amino Acid
sns gene
Time Factors
Transcription Factors - metabolism
title Drosophila SNS, a member of the immunoglobulin superfamily that is essential for myoblast fusion
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