$Frozen tissue can provide reproducible proteomic results of subcellular fractionation$

Frozen tissue can provide reproducible proteomic results of subcellular fractionation

Differential detergent fractionation (DDF) is frequently used to partition fresh cells and tissues into distinct compartments. We have tested whether DDF can reproducibly extract and fractionate cellular protein components from frozen tissues. Frozen kidneys were sequentially extracted with three di...

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Veröffentlicht in:	Analytical biochemistry 2011-11, Vol.418 (1), p.78-84
Hauptverfasser:	Lim, Jihyeon, Menon, Vilas, Bitzer, Markus, Miller, Leah M., Madrid-Aliste, Carlos, Weiss, Louis M., Fiser, Andras, Angeletti, Ruth H.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Cell Fractionation - methods cell membranes correlation Detergents - chemistry Differential detergent fractionation fractionation Freezing Frozen Sections Frozen tissue kidneys liquid chromatography Mice Mice, Inbred C57BL Normalized spectral index nuclear proteins proteome Proteome - analysis proteomics Proteomics - methods Subcellular Fractions - chemistry Subcellular location Tandem Mass Spectrometry tissues
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