Measuring the Bending Stiffness of Bacterial Cells Using an Optical Trap

We developed a protocol to measure the bending rigidity of filamentous rod-shaped bacteria. Forces are applied with an optical trap, a microscopic three-dimensional spring made of light that is formed when a high-intensity laser beam is focused to a very small spot by a microscope's objective l...

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Veröffentlicht in:	Journal of Visualized Experiments 2010-04 (38)
Hauptverfasser:	Wang, Siyuan, Arellano-Santoyo, Hugo, Combs, Peter A., Shaevitz, Joshua W.
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacteriological Techniques - methods Cephalexin - pharmacology Escherichia coli - drug effects Escherichia coli - growth & development Escherichia coli - physiology Microbiology Optical Tweezers Polylysine - chemistry
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