The effect of scaffold architecture on odontogenic differentiation of human dental pulp stem cells
Abstract Previous studies have shown the superiority of nanofibrous (NF) poly( l -lactic acid) (PLLA) scaffolds in supporting the osteogenic differentiation of a few cell types and bone regeneration. The aim of the current study was to investigate whether NF-PLLA scaffolds are advantageous for the o...
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| Veröffentlicht in: | Biomaterials 2011-11, Vol.32 (31), p.7822-7830 |
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| creator | Wang, Jing Ma, Haiyun Jin, Xiaobing Hu, Jiang Liu, Xiaohua Ni, Longxing Ma, Peter X |
| description | Abstract Previous studies have shown the superiority of nanofibrous (NF) poly( l -lactic acid) (PLLA) scaffolds in supporting the osteogenic differentiation of a few cell types and bone regeneration. The aim of the current study was to investigate whether NF-PLLA scaffolds are advantageous for the odontogenic differentiation and mineralization of human dental pulp stem cells (DPSCs) over solid-walled (SW) PLLA scaffolds. The in vitro studies demonstrated that, compared with SW scaffolds, NF scaffolds enhanced attachment and proliferation as well as odontogenic differentiation of human DPSCs. The alkaline phosphatase (ALP) activity and the expression of odontogenic genes of human DPSCs were increased on NF scaffolds compared with that on SW scaffolds. In addition, more mineral deposition was observed on the NF scaffolds, as demonstrated by von Kossa staining, calcium content measurement and scanning electron microscopy. Consistent with the in vitro studies, NF scaffolds promoted odontogenic differentiation and hard tissue formation compared with SW scaffolds after 8 weeks of ectopic transplantation in nude mice, as confirmed by von Kossa staining, Masson’s trichrome staining and immunohistochemical staining for dentin sialoprotein. In conclusion, NF-PLLA scaffolds enhanced the odontogenic differentiation of human DPSCs and mineralization both in vitro and in vivo , and are promising scaffolds for dentin regeneration. |
| doi_str_mv | 10.1016/j.biomaterials.2011.04.034 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3159766</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>1_s2_0_S0142961211004418</els_id><sourcerecordid>1710232649</sourcerecordid><originalsourceid>FETCH-LOGICAL-c672t-be972cb95bfabbbbbe8b467d352388f11f8cfac95aa8f695d93e55fe5b8975733</originalsourceid><addsrcrecordid>eNqNksFu1DAQhi0EosvCKyCLC1wSPE6c2BwqVYUCUiUOlLPlOOOulyRe7KRS3x5H21aFA8IXyzPfjGfmH0LeACuBQfN-X3Y-jGbG6M2QSs4ASlaXrKqfkA3IVhZCMfGUbBjUvFAN8BPyIqU9y29W8-fkhEPTVKrhG9Jd7ZCic2hnGhxN1jgXhp6aaHd-ztYlIg0TDX2Y5nCNk7e095mPOM3ezH71ObpbRjPRPtvMQA_LcKBpxpFaHIb0kjxzuU58dXdvyY-LT1fnX4rLb5-_np9dFrZp-Vx0qFpuOyU6Z7r1oOzqpu0rwSspHYCT1hmrhDHSNUr0qkIhHIpOqla0VbUlp8e8h6Ubsbe5mGgGfYh-NPFWB-P1n57J7_R1uNEVCNXmgWzJ27sEMfxaMM169GltwUwYlqQVa0EKzlgm3_2ThBYYr3hTq4x-OKI2hpQiuoeCgOlVTr3Xj-XUq5ya1TrLmYNfP27pIfRevwx8PAKYB3vjMepkPU4Wex-zeLoP_v_-Of0rjR18ltoMP_EW0z4scVpjQCeumf6-Lta6VwCM1TXI6jdXfNA8</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1710232649</pqid></control><display><type>article</type><title>The effect of scaffold architecture on odontogenic differentiation of human dental pulp stem cells</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Wang, Jing ; Ma, Haiyun ; Jin, Xiaobing ; Hu, Jiang ; Liu, Xiaohua ; Ni, Longxing ; Ma, Peter X</creator><creatorcontrib>Wang, Jing ; Ma, Haiyun ; Jin, Xiaobing ; Hu, Jiang ; Liu, Xiaohua ; Ni, Longxing ; Ma, Peter X</creatorcontrib><description>Abstract Previous studies have shown the superiority of nanofibrous (NF) poly( l -lactic acid) (PLLA) scaffolds in supporting the osteogenic differentiation of a few cell types and bone regeneration. The aim of the current study was to investigate whether NF-PLLA scaffolds are advantageous for the odontogenic differentiation and mineralization of human dental pulp stem cells (DPSCs) over solid-walled (SW) PLLA scaffolds. The in vitro studies demonstrated that, compared with SW scaffolds, NF scaffolds enhanced attachment and proliferation as well as odontogenic differentiation of human DPSCs. The alkaline phosphatase (ALP) activity and the expression of odontogenic genes of human DPSCs were increased on NF scaffolds compared with that on SW scaffolds. In addition, more mineral deposition was observed on the NF scaffolds, as demonstrated by von Kossa staining, calcium content measurement and scanning electron microscopy. Consistent with the in vitro studies, NF scaffolds promoted odontogenic differentiation and hard tissue formation compared with SW scaffolds after 8 weeks of ectopic transplantation in nude mice, as confirmed by von Kossa staining, Masson’s trichrome staining and immunohistochemical staining for dentin sialoprotein. In conclusion, NF-PLLA scaffolds enhanced the odontogenic differentiation of human DPSCs and mineralization both in vitro and in vivo , and are promising scaffolds for dentin regeneration.</description><identifier>ISSN: 0142-9612</identifier><identifier>EISSN: 1878-5905</identifier><identifier>DOI: 10.1016/j.biomaterials.2011.04.034</identifier><identifier>PMID: 21663962</identifier><language>eng</language><publisher>Netherlands: Elsevier Ltd</publisher><subject>Advanced Basic Science ; alkaline phosphatase ; Alkaline Phosphatase - metabolism ; Animals ; bone formation ; bone types ; Calcification, Physiologic - drug effects ; calcium ; Calcium - metabolism ; Cell Differentiation - drug effects ; Cell Proliferation - drug effects ; Cells, Cultured ; Dental Pulp - cytology ; Dental pulp stem cells (DPSCs) ; Dentistry ; Extracellular Matrix - drug effects ; Extracellular Matrix - metabolism ; Gene Expression Regulation - drug effects ; genes ; Humans ; Immunohistochemistry ; in vitro studies ; Lactic Acid - pharmacology ; Mice ; Mice, Nude ; Microscopy, Electron, Scanning ; Mineralization ; Nanofibrous poly( l-lactic acid) (NF-PLLA) scaffold ; Odontogenesis - drug effects ; Odontogenic differentiation ; Polyesters ; Polymers - pharmacology ; scanning electron microscopy ; Solid-walled (SW-PLLA) scaffold ; Staining and Labeling ; stem cells ; Stem Cells - cytology ; Stem Cells - drug effects ; Stem Cells - enzymology ; Stem Cells - ultrastructure ; Tissue Scaffolds - chemistry ; tooth pulp</subject><ispartof>Biomaterials, 2011-11, Vol.32 (31), p.7822-7830</ispartof><rights>Elsevier Ltd</rights><rights>2011 Elsevier Ltd</rights><rights>Copyright © 2011 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c672t-be972cb95bfabbbbbe8b467d352388f11f8cfac95aa8f695d93e55fe5b8975733</citedby><cites>FETCH-LOGICAL-c672t-be972cb95bfabbbbbe8b467d352388f11f8cfac95aa8f695d93e55fe5b8975733</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0142961211004418$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,776,780,881,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21663962$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Jing</creatorcontrib><creatorcontrib>Ma, Haiyun</creatorcontrib><creatorcontrib>Jin, Xiaobing</creatorcontrib><creatorcontrib>Hu, Jiang</creatorcontrib><creatorcontrib>Liu, Xiaohua</creatorcontrib><creatorcontrib>Ni, Longxing</creatorcontrib><creatorcontrib>Ma, Peter X</creatorcontrib><title>The effect of scaffold architecture on odontogenic differentiation of human dental pulp stem cells</title><title>Biomaterials</title><addtitle>Biomaterials</addtitle><description>Abstract Previous studies have shown the superiority of nanofibrous (NF) poly( l -lactic acid) (PLLA) scaffolds in supporting the osteogenic differentiation of a few cell types and bone regeneration. The aim of the current study was to investigate whether NF-PLLA scaffolds are advantageous for the odontogenic differentiation and mineralization of human dental pulp stem cells (DPSCs) over solid-walled (SW) PLLA scaffolds. The in vitro studies demonstrated that, compared with SW scaffolds, NF scaffolds enhanced attachment and proliferation as well as odontogenic differentiation of human DPSCs. The alkaline phosphatase (ALP) activity and the expression of odontogenic genes of human DPSCs were increased on NF scaffolds compared with that on SW scaffolds. In addition, more mineral deposition was observed on the NF scaffolds, as demonstrated by von Kossa staining, calcium content measurement and scanning electron microscopy. Consistent with the in vitro studies, NF scaffolds promoted odontogenic differentiation and hard tissue formation compared with SW scaffolds after 8 weeks of ectopic transplantation in nude mice, as confirmed by von Kossa staining, Masson’s trichrome staining and immunohistochemical staining for dentin sialoprotein. In conclusion, NF-PLLA scaffolds enhanced the odontogenic differentiation of human DPSCs and mineralization both in vitro and in vivo , and are promising scaffolds for dentin regeneration.</description><subject>Advanced Basic Science</subject><subject>alkaline phosphatase</subject><subject>Alkaline Phosphatase - metabolism</subject><subject>Animals</subject><subject>bone formation</subject><subject>bone types</subject><subject>Calcification, Physiologic - drug effects</subject><subject>calcium</subject><subject>Calcium - metabolism</subject><subject>Cell Differentiation - drug effects</subject><subject>Cell Proliferation - drug effects</subject><subject>Cells, Cultured</subject><subject>Dental Pulp - cytology</subject><subject>Dental pulp stem cells (DPSCs)</subject><subject>Dentistry</subject><subject>Extracellular Matrix - drug effects</subject><subject>Extracellular Matrix - metabolism</subject><subject>Gene Expression Regulation - drug effects</subject><subject>genes</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>in vitro studies</subject><subject>Lactic Acid - pharmacology</subject><subject>Mice</subject><subject>Mice, Nude</subject><subject>Microscopy, Electron, Scanning</subject><subject>Mineralization</subject><subject>Nanofibrous poly( l-lactic acid) (NF-PLLA) scaffold</subject><subject>Odontogenesis - drug effects</subject><subject>Odontogenic differentiation</subject><subject>Polyesters</subject><subject>Polymers - pharmacology</subject><subject>scanning electron microscopy</subject><subject>Solid-walled (SW-PLLA) scaffold</subject><subject>Staining and Labeling</subject><subject>stem cells</subject><subject>Stem Cells - cytology</subject><subject>Stem Cells - drug effects</subject><subject>Stem Cells - enzymology</subject><subject>Stem Cells - ultrastructure</subject><subject>Tissue Scaffolds - chemistry</subject><subject>tooth pulp</subject><issn>0142-9612</issn><issn>1878-5905</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNksFu1DAQhi0EosvCKyCLC1wSPE6c2BwqVYUCUiUOlLPlOOOulyRe7KRS3x5H21aFA8IXyzPfjGfmH0LeACuBQfN-X3Y-jGbG6M2QSs4ASlaXrKqfkA3IVhZCMfGUbBjUvFAN8BPyIqU9y29W8-fkhEPTVKrhG9Jd7ZCic2hnGhxN1jgXhp6aaHd-ztYlIg0TDX2Y5nCNk7e095mPOM3ezH71ObpbRjPRPtvMQA_LcKBpxpFaHIb0kjxzuU58dXdvyY-LT1fnX4rLb5-_np9dFrZp-Vx0qFpuOyU6Z7r1oOzqpu0rwSspHYCT1hmrhDHSNUr0qkIhHIpOqla0VbUlp8e8h6Ubsbe5mGgGfYh-NPFWB-P1n57J7_R1uNEVCNXmgWzJ27sEMfxaMM169GltwUwYlqQVa0EKzlgm3_2ThBYYr3hTq4x-OKI2hpQiuoeCgOlVTr3Xj-XUq5ya1TrLmYNfP27pIfRevwx8PAKYB3vjMepkPU4Wex-zeLoP_v_-Of0rjR18ltoMP_EW0z4scVpjQCeumf6-Lta6VwCM1TXI6jdXfNA8</recordid><startdate>20111101</startdate><enddate>20111101</enddate><creator>Wang, Jing</creator><creator>Ma, Haiyun</creator><creator>Jin, Xiaobing</creator><creator>Hu, Jiang</creator><creator>Liu, Xiaohua</creator><creator>Ni, Longxing</creator><creator>Ma, Peter X</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7S9</scope><scope>L.6</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>5PM</scope></search><sort><creationdate>20111101</creationdate><title>The effect of scaffold architecture on odontogenic differentiation of human dental pulp stem cells</title><author>Wang, Jing ; Ma, Haiyun ; Jin, Xiaobing ; Hu, Jiang ; Liu, Xiaohua ; Ni, Longxing ; Ma, Peter X</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c672t-be972cb95bfabbbbbe8b467d352388f11f8cfac95aa8f695d93e55fe5b8975733</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Advanced Basic Science</topic><topic>alkaline phosphatase</topic><topic>Alkaline Phosphatase - metabolism</topic><topic>Animals</topic><topic>bone formation</topic><topic>bone types</topic><topic>Calcification, Physiologic - drug effects</topic><topic>calcium</topic><topic>Calcium - metabolism</topic><topic>Cell Differentiation - drug effects</topic><topic>Cell Proliferation - drug effects</topic><topic>Cells, Cultured</topic><topic>Dental Pulp - cytology</topic><topic>Dental pulp stem cells (DPSCs)</topic><topic>Dentistry</topic><topic>Extracellular Matrix - drug effects</topic><topic>Extracellular Matrix - metabolism</topic><topic>Gene Expression Regulation - drug effects</topic><topic>genes</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>in vitro studies</topic><topic>Lactic Acid - pharmacology</topic><topic>Mice</topic><topic>Mice, Nude</topic><topic>Microscopy, Electron, Scanning</topic><topic>Mineralization</topic><topic>Nanofibrous poly( l-lactic acid) (NF-PLLA) scaffold</topic><topic>Odontogenesis - drug effects</topic><topic>Odontogenic differentiation</topic><topic>Polyesters</topic><topic>Polymers - pharmacology</topic><topic>scanning electron microscopy</topic><topic>Solid-walled (SW-PLLA) scaffold</topic><topic>Staining and Labeling</topic><topic>stem cells</topic><topic>Stem Cells - cytology</topic><topic>Stem Cells - drug effects</topic><topic>Stem Cells - enzymology</topic><topic>Stem Cells - ultrastructure</topic><topic>Tissue Scaffolds - chemistry</topic><topic>tooth pulp</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Jing</creatorcontrib><creatorcontrib>Ma, Haiyun</creatorcontrib><creatorcontrib>Jin, Xiaobing</creatorcontrib><creatorcontrib>Hu, Jiang</creatorcontrib><creatorcontrib>Liu, Xiaohua</creatorcontrib><creatorcontrib>Ni, Longxing</creatorcontrib><creatorcontrib>Ma, Peter X</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Biomaterials</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Jing</au><au>Ma, Haiyun</au><au>Jin, Xiaobing</au><au>Hu, Jiang</au><au>Liu, Xiaohua</au><au>Ni, Longxing</au><au>Ma, Peter X</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The effect of scaffold architecture on odontogenic differentiation of human dental pulp stem cells</atitle><jtitle>Biomaterials</jtitle><addtitle>Biomaterials</addtitle><date>2011-11-01</date><risdate>2011</risdate><volume>32</volume><issue>31</issue><spage>7822</spage><epage>7830</epage><pages>7822-7830</pages><issn>0142-9612</issn><eissn>1878-5905</eissn><abstract>Abstract Previous studies have shown the superiority of nanofibrous (NF) poly( l -lactic acid) (PLLA) scaffolds in supporting the osteogenic differentiation of a few cell types and bone regeneration. The aim of the current study was to investigate whether NF-PLLA scaffolds are advantageous for the odontogenic differentiation and mineralization of human dental pulp stem cells (DPSCs) over solid-walled (SW) PLLA scaffolds. The in vitro studies demonstrated that, compared with SW scaffolds, NF scaffolds enhanced attachment and proliferation as well as odontogenic differentiation of human DPSCs. The alkaline phosphatase (ALP) activity and the expression of odontogenic genes of human DPSCs were increased on NF scaffolds compared with that on SW scaffolds. In addition, more mineral deposition was observed on the NF scaffolds, as demonstrated by von Kossa staining, calcium content measurement and scanning electron microscopy. Consistent with the in vitro studies, NF scaffolds promoted odontogenic differentiation and hard tissue formation compared with SW scaffolds after 8 weeks of ectopic transplantation in nude mice, as confirmed by von Kossa staining, Masson’s trichrome staining and immunohistochemical staining for dentin sialoprotein. In conclusion, NF-PLLA scaffolds enhanced the odontogenic differentiation of human DPSCs and mineralization both in vitro and in vivo , and are promising scaffolds for dentin regeneration.</abstract><cop>Netherlands</cop><pub>Elsevier Ltd</pub><pmid>21663962</pmid><doi>10.1016/j.biomaterials.2011.04.034</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Advanced Basic Science alkaline phosphatase Alkaline Phosphatase - metabolism Animals bone formation bone types Calcification, Physiologic - drug effects calcium Calcium - metabolism Cell Differentiation - drug effects Cell Proliferation - drug effects Cells, Cultured Dental Pulp - cytology Dental pulp stem cells (DPSCs) Dentistry Extracellular Matrix - drug effects Extracellular Matrix - metabolism Gene Expression Regulation - drug effects genes Humans Immunohistochemistry in vitro studies Lactic Acid - pharmacology Mice Mice, Nude Microscopy, Electron, Scanning Mineralization Nanofibrous poly( l-lactic acid) (NF-PLLA) scaffold Odontogenesis - drug effects Odontogenic differentiation Polyesters Polymers - pharmacology scanning electron microscopy Solid-walled (SW-PLLA) scaffold Staining and Labeling stem cells Stem Cells - cytology Stem Cells - drug effects Stem Cells - enzymology Stem Cells - ultrastructure Tissue Scaffolds - chemistry tooth pulp |
| title | The effect of scaffold architecture on odontogenic differentiation of human dental pulp stem cells |
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