Quantification of collagen organization using fractal dimensions and Fourier transforms
Collagen fibers and fibrils that comprise tendons and ligaments are disrupted or damaged during injury. Fibrillogenesis during healing produces a matrix that is initially quite disorganized, but remodels over time to resemble, but not replicate, the original roughly parallel microstructure. Quantifi...
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| Veröffentlicht in: | Acta histochemica 2012-02, Vol.114 (2), p.140-144 |
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| container_title | Acta histochemica |
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| creator | Frisch, Kayt E. Duenwald-Kuehl, Sarah E. Kobayashi, Hirohito Chamberlain, Connie S. Lakes, Roderic S. Vanderby, Ray |
| description | Collagen fibers and fibrils that comprise tendons and ligaments are disrupted or damaged during injury. Fibrillogenesis during healing produces a matrix that is initially quite disorganized, but remodels over time to resemble, but not replicate, the original roughly parallel microstructure. Quantification of these changes is traditionally a laborious and subjective task. In this work we applied two automated techniques, fast Fourier transformation (FFT) and fractal dimension analysis (FA) to quantify the organization of collagen fibers or fibrils. Using multi-photon images of collagen fibers obtained from rat ligament we showed that for healing ligaments, FA differentiates more clearly between the different time-points during healing. Using scanning electron microscopy images of overstretched porcine flexor tendon, we showed that combining FFT and FA measures distinguishes the damaged and undamaged groups more clearly than either method separately. |
| doi_str_mv | 10.1016/j.acthis.2011.03.010 |
| format | Article |
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Fibrillogenesis during healing produces a matrix that is initially quite disorganized, but remodels over time to resemble, but not replicate, the original roughly parallel microstructure. Quantification of these changes is traditionally a laborious and subjective task. In this work we applied two automated techniques, fast Fourier transformation (FFT) and fractal dimension analysis (FA) to quantify the organization of collagen fibers or fibrils. Using multi-photon images of collagen fibers obtained from rat ligament we showed that for healing ligaments, FA differentiates more clearly between the different time-points during healing. 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All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c462t-80d47f613caa4a1a174dbdf6513d0397cae6eb95deef196b521822496766c2dc3</citedby><cites>FETCH-LOGICAL-c462t-80d47f613caa4a1a174dbdf6513d0397cae6eb95deef196b521822496766c2dc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.acthis.2011.03.010$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,780,784,885,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21529898$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Frisch, Kayt E.</creatorcontrib><creatorcontrib>Duenwald-Kuehl, Sarah E.</creatorcontrib><creatorcontrib>Kobayashi, Hirohito</creatorcontrib><creatorcontrib>Chamberlain, Connie S.</creatorcontrib><creatorcontrib>Lakes, Roderic S.</creatorcontrib><creatorcontrib>Vanderby, Ray</creatorcontrib><title>Quantification of collagen organization using fractal dimensions and Fourier transforms</title><title>Acta histochemica</title><addtitle>Acta Histochem</addtitle><description>Collagen fibers and fibrils that comprise tendons and ligaments are disrupted or damaged during injury. Fibrillogenesis during healing produces a matrix that is initially quite disorganized, but remodels over time to resemble, but not replicate, the original roughly parallel microstructure. Quantification of these changes is traditionally a laborious and subjective task. In this work we applied two automated techniques, fast Fourier transformation (FFT) and fractal dimension analysis (FA) to quantify the organization of collagen fibers or fibrils. Using multi-photon images of collagen fibers obtained from rat ligament we showed that for healing ligaments, FA differentiates more clearly between the different time-points during healing. Using scanning electron microscopy images of overstretched porcine flexor tendon, we showed that combining FFT and FA measures distinguishes the damaged and undamaged groups more clearly than either method separately.</description><subject>Animals</subject><subject>Collagen</subject><subject>Collagen - metabolism</subject><subject>Collagen - ultrastructure</subject><subject>FFT</subject><subject>Fourier Analysis</subject><subject>Fourier transform</subject><subject>Fractal</subject><subject>Fractals</subject><subject>Ligament</subject><subject>Male</subject><subject>Medial Collateral Ligament, Knee - metabolism</subject><subject>Medial Collateral Ligament, Knee - pathology</subject><subject>Medial Collateral Ligament, Knee - ultrastructure</subject><subject>Microscopy, Electron, Scanning</subject><subject>Microscopy, Fluorescence, Multiphoton</subject><subject>Models, Biological</subject><subject>Pig</subject><subject>Rat</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Tendon</subject><subject>Wound Healing</subject><issn>0065-1281</issn><issn>1618-0372</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU9r3DAQxUVpaLZpv0EpvvVkRyPZsnUJlJA_hUAIpPQotNJoo8WWEskONJ8-WjZN20tOEpqZ90bvR8gXoA1QEMfbRpv5zueGUYCG8oYCfUdWIGCoKe_Ze7KiVHQ1sAEOycect5RSSTn7QA4ZdEwOcliRXzeLDrN33ujZx1BFV5k4jnqD5Z42OvinfWHJPmwql4qpHivrJwy5vOdKB1udxyV5TNWcdMgupil_IgdOjxk_v5xH5Of52e3pZX11ffHj9PtVbVrB5nqgtu2dAG60bjVo6Fu7tk50wC3lsjcaBa5lZxEdSLHuGAyMtVL0QhhmDT8iJ3vd-2U9oTUYyg6juk9-0um3itqr_yvB36lNfFQcukF2rAh8exFI8WHBPKvJZ4MlgoBxyUqCZGxo5a6z3XeaFHNO6F5dgKodErVVeyRqh0RRrgqSMvb13w1fh_4w-PsFLDk9lhhVNh6DQesTmlnZ6N92eAbsGaIU</recordid><startdate>201202</startdate><enddate>201202</enddate><creator>Frisch, Kayt E.</creator><creator>Duenwald-Kuehl, Sarah E.</creator><creator>Kobayashi, Hirohito</creator><creator>Chamberlain, Connie S.</creator><creator>Lakes, Roderic S.</creator><creator>Vanderby, Ray</creator><general>Elsevier GmbH</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>201202</creationdate><title>Quantification of collagen organization using fractal dimensions and Fourier transforms</title><author>Frisch, Kayt E. ; Duenwald-Kuehl, Sarah E. ; Kobayashi, Hirohito ; Chamberlain, Connie S. ; Lakes, Roderic S. ; Vanderby, Ray</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c462t-80d47f613caa4a1a174dbdf6513d0397cae6eb95deef196b521822496766c2dc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Animals</topic><topic>Collagen</topic><topic>Collagen - metabolism</topic><topic>Collagen - ultrastructure</topic><topic>FFT</topic><topic>Fourier Analysis</topic><topic>Fourier transform</topic><topic>Fractal</topic><topic>Fractals</topic><topic>Ligament</topic><topic>Male</topic><topic>Medial Collateral Ligament, Knee - metabolism</topic><topic>Medial Collateral Ligament, Knee - pathology</topic><topic>Medial Collateral Ligament, Knee - ultrastructure</topic><topic>Microscopy, Electron, Scanning</topic><topic>Microscopy, Fluorescence, Multiphoton</topic><topic>Models, Biological</topic><topic>Pig</topic><topic>Rat</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Tendon</topic><topic>Wound Healing</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Frisch, Kayt E.</creatorcontrib><creatorcontrib>Duenwald-Kuehl, Sarah E.</creatorcontrib><creatorcontrib>Kobayashi, Hirohito</creatorcontrib><creatorcontrib>Chamberlain, Connie S.</creatorcontrib><creatorcontrib>Lakes, Roderic S.</creatorcontrib><creatorcontrib>Vanderby, Ray</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Acta histochemica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Frisch, Kayt E.</au><au>Duenwald-Kuehl, Sarah E.</au><au>Kobayashi, Hirohito</au><au>Chamberlain, Connie S.</au><au>Lakes, Roderic S.</au><au>Vanderby, Ray</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Quantification of collagen organization using fractal dimensions and Fourier transforms</atitle><jtitle>Acta histochemica</jtitle><addtitle>Acta Histochem</addtitle><date>2012-02</date><risdate>2012</risdate><volume>114</volume><issue>2</issue><spage>140</spage><epage>144</epage><pages>140-144</pages><issn>0065-1281</issn><eissn>1618-0372</eissn><abstract>Collagen fibers and fibrils that comprise tendons and ligaments are disrupted or damaged during injury. 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| identifier | ISSN: 0065-1281 |
| ispartof | Acta histochemica, 2012-02, Vol.114 (2), p.140-144 |
| issn | 0065-1281 1618-0372 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Animals Collagen Collagen - metabolism Collagen - ultrastructure FFT Fourier Analysis Fourier transform Fractal Fractals Ligament Male Medial Collateral Ligament, Knee - metabolism Medial Collateral Ligament, Knee - pathology Medial Collateral Ligament, Knee - ultrastructure Microscopy, Electron, Scanning Microscopy, Fluorescence, Multiphoton Models, Biological Pig Rat Rats Rats, Wistar Tendon Wound Healing |
| title | Quantification of collagen organization using fractal dimensions and Fourier transforms |
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