An Examination by Site-Directed Mutagenesis of Putative Key Residues in the Determination of Coenzyme Specificity in Clostridial NAD^+-Dependent Glutamate Dehydrogenase

Sequence and structure comparisons of various glutamate dehydrogenases (GDH) and other nicotinamide nucleotide-dependent dehydrogenases have potentially implicated certain residues in coenzyme binding and discrimination. We have mutated key residues in Clostridium symbiosum NAD+-specific GDH to inve...

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Veröffentlicht in:	Enzyme Research 2011, Vol.2011 (2011), p.623-631
Hauptverfasser:	Griffin, Joanna, Engel, Paul C.
Format:	Artikel
Sprache:	eng
Online-Zugang:	Volltext
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