Characterization of Human Fungiform Papillae Cells in Culture
The ability to maintain human fungiform papillae cells in culture for multiple cell cycles would be of considerable utility for characterizing the molecular, regenerative, and functional properties of these unique sensory cells. Here we describe a method for enzymatically isolating human cells from...
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| Veröffentlicht in: | Chemical senses 2011-09, Vol.36 (7), p.601-612 |
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| container_title | Chemical senses |
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| creator | Ozdener, Mehmet Hakan Brand, Joseph G Spielman, Andrew I Lischka, Fritz W Teeter, John H Breslin, Paul A.S Rawson, Nancy E |
| description | The ability to maintain human fungiform papillae cells in culture for multiple cell cycles would be of considerable utility for characterizing the molecular, regenerative, and functional properties of these unique sensory cells. Here we describe a method for enzymatically isolating human cells from fungiform papillae obtained by biopsy and maintaining them in culture for more than 7 passages (7 months) without loss of viability and while retaining many of the functional properties of acutely isolated taste cells. Cells in these cultures exhibited increases in intracellular calcium when stimulated with perceptually appropriate concentrations of several taste stimuli, indicating that at least some of the native signaling pathways were present. This system can provide a useful model for molecular studies of the proliferation, differentiation, and physiological function of human fungiform papillae cells. |
| doi_str_mv | 10.1093/chemse/bjr012 |
| format | Article |
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Here we describe a method for enzymatically isolating human cells from fungiform papillae obtained by biopsy and maintaining them in culture for more than 7 passages (7 months) without loss of viability and while retaining many of the functional properties of acutely isolated taste cells. Cells in these cultures exhibited increases in intracellular calcium when stimulated with perceptually appropriate concentrations of several taste stimuli, indicating that at least some of the native signaling pathways were present. This system can provide a useful model for molecular studies of the proliferation, differentiation, and physiological function of human fungiform papillae cells.</description><identifier>ISSN: 0379-864X</identifier><identifier>EISSN: 1464-3553</identifier><identifier>DOI: 10.1093/chemse/bjr012</identifier><identifier>PMID: 21471186</identifier><identifier>CODEN: CHSED8</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>Adult ; Biological and medical sciences ; biopsy ; calcium ; Cell Cycle ; Cells, Cultured ; cytology ; Female ; functional properties ; Fundamental and applied biological sciences. Psychology ; Humans ; Male ; Middle Aged ; Olfaction. Taste ; Perception ; physiology ; Psychology. Psychoanalysis. Psychiatry ; Psychology. Psychophysiology ; signal transduction ; Taste ; Taste Buds ; Taste Buds - cytology ; Taste Buds - physiology ; Tissue Donors ; viability</subject><ispartof>Chemical senses, 2011-09, Vol.36 (7), p.601-612</ispartof><rights>The Author 2011. Published by Oxford University Press. All rights reserved. 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Here we describe a method for enzymatically isolating human cells from fungiform papillae obtained by biopsy and maintaining them in culture for more than 7 passages (7 months) without loss of viability and while retaining many of the functional properties of acutely isolated taste cells. Cells in these cultures exhibited increases in intracellular calcium when stimulated with perceptually appropriate concentrations of several taste stimuli, indicating that at least some of the native signaling pathways were present. This system can provide a useful model for molecular studies of the proliferation, differentiation, and physiological function of human fungiform papillae cells.</description><subject>Adult</subject><subject>Biological and medical sciences</subject><subject>biopsy</subject><subject>calcium</subject><subject>Cell Cycle</subject><subject>Cells, Cultured</subject><subject>cytology</subject><subject>Female</subject><subject>functional properties</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Olfaction. Taste</subject><subject>Perception</subject><subject>physiology</subject><subject>Psychology. Psychoanalysis. Psychiatry</subject><subject>Psychology. Psychophysiology</subject><subject>signal transduction</subject><subject>Taste</subject><subject>Taste Buds</subject><subject>Taste Buds - cytology</subject><subject>Taste Buds - physiology</subject><subject>Tissue Donors</subject><subject>viability</subject><issn>0379-864X</issn><issn>1464-3553</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFks1rFTEUxYNY7LO6dKuzKboZm-9JFgplsK1QUNCCu3AnL3kvZWbymswU6l9vZJ6tbuwqi_vj5Jx7LkKvCH5PsGYnduuG7E6664QJfYJWhEteMyHYU7TCrNG1kvzHIXqe8zXGhDOqnqFDSnhDiJIr9KHdQgI7uRR-whTiWEVfXcwDjNXZPG6Cj2movsIu9D24qnV9n6swVu3cT3NyL9CBhz67l_v3CF2dffreXtSXX84_t6eXtRVMTXVHKFOqA82s7horuezomhAoQ0cAgDIPQnZM0mJc-wbIWlIvhFWgnbKeHaGPi-5u7ga3tm6cEvRml8IA6c5ECObfyRi2ZhNvDSNlE5oUgbd7gRRvZpcnM4RsSxoYXZyz0ZiyBmvRPEoqxZQgXPFCvvsvSaQsiTDnrKD1gtoUc07O31sn2Pyu0Sw1mqXGwr_-O-89_ae3AhzvAcgWep9gtCE_cJzTRnH54DHOu0f_fLOgHqKBTSpyV99oOZlyNqxRArNfdD--Vw</recordid><startdate>20110901</startdate><enddate>20110901</enddate><creator>Ozdener, Mehmet Hakan</creator><creator>Brand, Joseph G</creator><creator>Spielman, Andrew I</creator><creator>Lischka, Fritz W</creator><creator>Teeter, John H</creator><creator>Breslin, Paul A.S</creator><creator>Rawson, Nancy E</creator><general>Oxford University Press</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7S9</scope><scope>L.6</scope><scope>7X8</scope><scope>7QR</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>5PM</scope></search><sort><creationdate>20110901</creationdate><title>Characterization of Human Fungiform Papillae Cells in Culture</title><author>Ozdener, Mehmet Hakan ; Brand, Joseph G ; Spielman, Andrew I ; Lischka, Fritz W ; Teeter, John H ; Breslin, Paul A.S ; Rawson, Nancy E</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c538t-b12388ba93c9b7c646b2d11a538e1aaa23fa56b3623559f7a1d62f55c8a9e8cf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Adult</topic><topic>Biological and medical sciences</topic><topic>biopsy</topic><topic>calcium</topic><topic>Cell Cycle</topic><topic>Cells, Cultured</topic><topic>cytology</topic><topic>Female</topic><topic>functional properties</topic><topic>Fundamental and applied biological sciences. 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Here we describe a method for enzymatically isolating human cells from fungiform papillae obtained by biopsy and maintaining them in culture for more than 7 passages (7 months) without loss of viability and while retaining many of the functional properties of acutely isolated taste cells. Cells in these cultures exhibited increases in intracellular calcium when stimulated with perceptually appropriate concentrations of several taste stimuli, indicating that at least some of the native signaling pathways were present. This system can provide a useful model for molecular studies of the proliferation, differentiation, and physiological function of human fungiform papillae cells.</abstract><cop>Oxford</cop><pub>Oxford University Press</pub><pmid>21471186</pmid><doi>10.1093/chemse/bjr012</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
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| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Adult Biological and medical sciences biopsy calcium Cell Cycle Cells, Cultured cytology Female functional properties Fundamental and applied biological sciences. Psychology Humans Male Middle Aged Olfaction. Taste Perception physiology Psychology. Psychoanalysis. Psychiatry Psychology. Psychophysiology signal transduction Taste Taste Buds Taste Buds - cytology Taste Buds - physiology Tissue Donors viability |
| title | Characterization of Human Fungiform Papillae Cells in Culture |
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