Microfluidic cell counter with embedded optical fibers fabricated by femtosecond laser ablation and anodic bonding
A simple fabrication technique to create all silicon/glass microfluidic devices is demonstrated using femtosecond laser ablation and anodic bonding. In a first application, we constructed a cell counting device based on small angle light scattering. The counter featured embedded optical fibers for m...
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| Veröffentlicht in: | Optics express 2009-04, Vol.17 (8), p.6068-6073 |
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| container_issue | 8 |
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| container_title | Optics express |
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| creator | Schafer, Dawn Gibson, Emily A Salim, Evan A Palmer, Amy E Jimenez, Ralph Squier, Jeff |
| description | A simple fabrication technique to create all silicon/glass microfluidic devices is demonstrated using femtosecond laser ablation and anodic bonding. In a first application, we constructed a cell counting device based on small angle light scattering. The counter featured embedded optical fibers for multiangle excitation and detection of scattered light and/or fluorescence. The performance of the microfluidic cell counter was benchmarked against a commercial fluorescence-activated cell sorter. |
| doi_str_mv | 10.1364/OE.17.006068 |
| format | Article |
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| identifier | ISSN: 1094-4087 |
| ispartof | Optics express, 2009-04, Vol.17 (8), p.6068-6073 |
| issn | 1094-4087 1094-4087 |
| language | eng |
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| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Cell Count - instrumentation Cell Separation - instrumentation Electrodes Equipment Design Equipment Failure Analysis Fiber Optic Technology - instrumentation Lasers Microfluidic Analytical Techniques - instrumentation Refractometry - instrumentation Reproducibility of Results Sensitivity and Specificity |
| title | Microfluidic cell counter with embedded optical fibers fabricated by femtosecond laser ablation and anodic bonding |
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