Determination of nucleoside analog mono-, di-, and tri-phosphates in cellular matrix by solid phase extraction and ultra-sensitive LC–MS/MS detection

An ultra-sensitive liquid chromatography tandem mass spectrometry (LC–MS/MS) assay was developed and validated to facilitate the assessment of clinical pharmacokinetics of nucleotide analogs from lysed intracellular matrix. The method utilized a strong anion exchange isolation of mono-(MP), di-(DP),...

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Veröffentlicht in:	Journal of pharmaceutical and biomedical analysis 2011-09, Vol.56 (2), p.390-401
Hauptverfasser:	Bushman, Lane R., Kiser, Jennifer J., Rower, Joseph E., Klein, Brandon, Zheng, Jia-Hua, Ray, Michelle L., Anderson, Peter L.
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenine - analogs & derivatives Adenine - isolation & purification Analysis Analytical methods Analytical, structural and metabolic biochemistry anion exchange Anion Exchange Resins Anions Antiviral Agents - isolation & purification Biological and medical sciences Calibration Chromatography, Liquid - standards Clinical pharmacology Deoxycytidine - analogs & derivatives Deoxycytidine - isolation & purification Diphosphates - isolation & purification Emtricitabine Fundamental and applied biological sciences. Psychology General pharmacology Humans Intracellular pharmacology Lamivudine Lamivudine - isolation & purification LC–MS/MS Leukocytes, Mononuclear - chemistry Liquid chromatography Mass spectroscopy Medical sciences Nucleoside analogs nucleosides Nucleosides - isolation & purification Nucleosides - pharmacokinetics Nucleotide analogs Nucleotides Organophosphonates - isolation & purification Pharmacokinetics Pharmacology. Drug treatments Phosphates - isolation & purification Phosphates - pharmacokinetics Reference Standards Reproducibility of Results Sensitivity and Specificity solid phase extraction Solid Phase Extraction - standards tandem mass spectrometry Tandem Mass Spectrometry - standards Tenofovir Zidovudine Zidovudine - isolation & purification
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