Osteogenic oxysterol, 20(S)‐hydroxycholesterol, induces notch target gene expression in bone marrow stromal cells
We previously reported that specific oxysterols stimulate osteogenic differentiation of pluripotent bone marrow stromal cells (MSCs) through activation of hedgehog (Hh) signaling and may serve as potential future therapies for intervention in osteopenia and osteoporosis. In this study we report that...
Gespeichert in:
| Veröffentlicht in: | Journal of bone and mineral research 2010-04, Vol.25 (4), p.782-795 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 795 |
|---|---|
| container_issue | 4 |
| container_start_page | 782 |
| container_title | Journal of bone and mineral research |
| container_volume | 25 |
| creator | Kim, Woo‐Kyun Meliton, Vicente Tetradis, Sotirios Weinmaster, Gerry Hahn, Theodore J Carlson, Marc Nelson, Stanley F Parhami, Farhad |
| description | We previously reported that specific oxysterols stimulate osteogenic differentiation of pluripotent bone marrow stromal cells (MSCs) through activation of hedgehog (Hh) signaling and may serve as potential future therapies for intervention in osteopenia and osteoporosis. In this study we report that the osteogenic oxysterol 20(S)‐hydroxycholesterol (20S) induces the expression of genes associated with Notch signaling. Using M2‐10B4 (M2) MSCs, we found that 20S significantly induced HES‐1, HEY‐1, and HEY‐2 mRNA expression compared with untreated cells, with maximal induction after 48 hours, whereas the nonosteogenic oxysterols did not. Similar observations were made when M2 cells were treated with sonic hedgehog (Shh), and the specific Hh pathway inhibitor cyclopamine blocked 20S‐induced Notch target gene expression. 20S did not induce Notch target genes in Smo−/− mouse embryonic fibroblasts, further confirming the role of Hh signaling in 20S‐induced expression of Notch target genes. Despite the inability of liver X‐receptor (LXR) synthetic ligand TO901317 to induce Notch target genes in M2 cells, LXR knockdown studies using siRNA showed inhibition of 20S‐induced HEY‐1 but not HES‐1 expression, suggesting the partial role of LXR signaling in MSC responses to 20S. Moreover, 20S‐induced Notch target gene expression was independent of canonical Notch signaling because neither 20S nor Shh induced CBF1 luciferase reporter activity or NICD protein accumulation in the nucleus, which are hallmarks of canonical Notch signaling activation. Finally, HES‐1 and HEY‐1 siRNA transfection significantly inhibited 20S‐induced osteogenic genes, suggesting that the pro‐osteogenic effects of 20S are regulated in part by HES‐1 and HEY‐1. © 2010 American Society for Bone and Mineral Research |
| doi_str_mv | 10.1359/jbmr.091024 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3153332</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>745928447</sourcerecordid><originalsourceid>FETCH-LOGICAL-c6124-e1aca504e0cb5914ca518f165cf9efdd4f4e8ebee66ccf717275f98a65352fd73</originalsourceid><addsrcrecordid>eNp9kctu1DAUhi0EokNhxR5FQhVFkOJ7nA0SVFxVVInL2nKc4xmPkniwE9rZ8Qg8I0-CRxnKZdHV0Tnn038uP0L3CT4hTNTP1k0fT3BNMOU30IIIykouFbmJFlgpXmLOyAG6k9IaYyyFlLfRAakVq6tKLlA6TyOEJQzeFuFym5MYuqcFxcefHv_8_mO1bWMu21Xo4HfPD-1kIRVDGO2qGE1cwlhkBSjgchMhJR-GDBVNyKXexBguijTG0JuusNB16S665UyX4N4-HqIvr199Pn1bnp2_eXf64qy0klBeAjHWCMwB20bUhOeEKEeksK4G17bccVDQAEhpratIRSvhamWkYIK6tmKH6Pmsu5maHloLwxhNpzfR5622Ohiv_-0MfqWX4ZtmRDDGaBZ4tBeI4euUH6B7n3YnmAHClHTFRU0V57tRx9eSRFVUMSWFyujD_9B1mOKQH5EpKQUhhOJMPZkpG0NKEdzV2gTrne16Z7uebc_0g78v_cPufc7A0R4wyZrORTNYn644ShUV-YGZozN34TvYXjdTv3_54eM8_Rewp8jk</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1866511120</pqid></control><display><type>article</type><title>Osteogenic oxysterol, 20(S)‐hydroxycholesterol, induces notch target gene expression in bone marrow stromal cells</title><source>MEDLINE</source><source>Access via Wiley Online Library</source><source>Oxford University Press Journals All Titles (1996-Current)</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Alma/SFX Local Collection</source><creator>Kim, Woo‐Kyun ; Meliton, Vicente ; Tetradis, Sotirios ; Weinmaster, Gerry ; Hahn, Theodore J ; Carlson, Marc ; Nelson, Stanley F ; Parhami, Farhad</creator><creatorcontrib>Kim, Woo‐Kyun ; Meliton, Vicente ; Tetradis, Sotirios ; Weinmaster, Gerry ; Hahn, Theodore J ; Carlson, Marc ; Nelson, Stanley F ; Parhami, Farhad</creatorcontrib><description>We previously reported that specific oxysterols stimulate osteogenic differentiation of pluripotent bone marrow stromal cells (MSCs) through activation of hedgehog (Hh) signaling and may serve as potential future therapies for intervention in osteopenia and osteoporosis. In this study we report that the osteogenic oxysterol 20(S)‐hydroxycholesterol (20S) induces the expression of genes associated with Notch signaling. Using M2‐10B4 (M2) MSCs, we found that 20S significantly induced HES‐1, HEY‐1, and HEY‐2 mRNA expression compared with untreated cells, with maximal induction after 48 hours, whereas the nonosteogenic oxysterols did not. Similar observations were made when M2 cells were treated with sonic hedgehog (Shh), and the specific Hh pathway inhibitor cyclopamine blocked 20S‐induced Notch target gene expression. 20S did not induce Notch target genes in Smo−/− mouse embryonic fibroblasts, further confirming the role of Hh signaling in 20S‐induced expression of Notch target genes. Despite the inability of liver X‐receptor (LXR) synthetic ligand TO901317 to induce Notch target genes in M2 cells, LXR knockdown studies using siRNA showed inhibition of 20S‐induced HEY‐1 but not HES‐1 expression, suggesting the partial role of LXR signaling in MSC responses to 20S. Moreover, 20S‐induced Notch target gene expression was independent of canonical Notch signaling because neither 20S nor Shh induced CBF1 luciferase reporter activity or NICD protein accumulation in the nucleus, which are hallmarks of canonical Notch signaling activation. Finally, HES‐1 and HEY‐1 siRNA transfection significantly inhibited 20S‐induced osteogenic genes, suggesting that the pro‐osteogenic effects of 20S are regulated in part by HES‐1 and HEY‐1. © 2010 American Society for Bone and Mineral Research</description><identifier>ISSN: 0884-0431</identifier><identifier>EISSN: 1523-4681</identifier><identifier>DOI: 10.1359/jbmr.091024</identifier><identifier>PMID: 19839776</identifier><identifier>CODEN: JBMREJ</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Animals ; Basic Helix-Loop-Helix Transcription Factors - analysis ; Basic Helix-Loop-Helix Transcription Factors - metabolism ; Biological and medical sciences ; Bone Marrow Cells - drug effects ; Bone Marrow Cells - metabolism ; Cell Culture Techniques ; Cell Cycle Proteins - analysis ; Cell Cycle Proteins - metabolism ; Fibroblasts - drug effects ; Fibroblasts - metabolism ; Fundamental and applied biological sciences. Psychology ; Gene Expression - drug effects ; hedgehog ; Hedgehog Proteins - metabolism ; Homeodomain Proteins - analysis ; Homeodomain Proteins - metabolism ; Hydroxycholesterols - pharmacology ; Liver X Receptors ; mesenchymal stem cells ; Mice ; Notch ; Original ; Orphan Nuclear Receptors - analysis ; Orphan Nuclear Receptors - metabolism ; osteogenesis ; Osteogenesis - drug effects ; oxysterol ; Receptors, Notch - genetics ; RNA, Messenger - analysis ; RNA, Messenger - metabolism ; Skeleton and joints ; Stromal Cells - drug effects ; Stromal Cells - metabolism ; Transcription Factor HES-1 ; Veratrum Alkaloids - pharmacology ; Vertebrates: osteoarticular system, musculoskeletal system</subject><ispartof>Journal of bone and mineral research, 2010-04, Vol.25 (4), p.782-795</ispartof><rights>Copyright © 2010 American Society for Bone and Mineral Research</rights><rights>2015 INIST-CNRS</rights><rights>Copyright 2010 American Society for Bone and Mineral Research.</rights><rights>Copyright © 2010 American Society for Bone and Mineral Research 2010</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c6124-e1aca504e0cb5914ca518f165cf9efdd4f4e8ebee66ccf717275f98a65352fd73</citedby><cites>FETCH-LOGICAL-c6124-e1aca504e0cb5914ca518f165cf9efdd4f4e8ebee66ccf717275f98a65352fd73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1359%2Fjbmr.091024$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1359%2Fjbmr.091024$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>230,315,781,785,886,1418,27929,27930,45579,45580</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=22825172$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19839776$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kim, Woo‐Kyun</creatorcontrib><creatorcontrib>Meliton, Vicente</creatorcontrib><creatorcontrib>Tetradis, Sotirios</creatorcontrib><creatorcontrib>Weinmaster, Gerry</creatorcontrib><creatorcontrib>Hahn, Theodore J</creatorcontrib><creatorcontrib>Carlson, Marc</creatorcontrib><creatorcontrib>Nelson, Stanley F</creatorcontrib><creatorcontrib>Parhami, Farhad</creatorcontrib><title>Osteogenic oxysterol, 20(S)‐hydroxycholesterol, induces notch target gene expression in bone marrow stromal cells</title><title>Journal of bone and mineral research</title><addtitle>J Bone Miner Res</addtitle><description>We previously reported that specific oxysterols stimulate osteogenic differentiation of pluripotent bone marrow stromal cells (MSCs) through activation of hedgehog (Hh) signaling and may serve as potential future therapies for intervention in osteopenia and osteoporosis. In this study we report that the osteogenic oxysterol 20(S)‐hydroxycholesterol (20S) induces the expression of genes associated with Notch signaling. Using M2‐10B4 (M2) MSCs, we found that 20S significantly induced HES‐1, HEY‐1, and HEY‐2 mRNA expression compared with untreated cells, with maximal induction after 48 hours, whereas the nonosteogenic oxysterols did not. Similar observations were made when M2 cells were treated with sonic hedgehog (Shh), and the specific Hh pathway inhibitor cyclopamine blocked 20S‐induced Notch target gene expression. 20S did not induce Notch target genes in Smo−/− mouse embryonic fibroblasts, further confirming the role of Hh signaling in 20S‐induced expression of Notch target genes. Despite the inability of liver X‐receptor (LXR) synthetic ligand TO901317 to induce Notch target genes in M2 cells, LXR knockdown studies using siRNA showed inhibition of 20S‐induced HEY‐1 but not HES‐1 expression, suggesting the partial role of LXR signaling in MSC responses to 20S. Moreover, 20S‐induced Notch target gene expression was independent of canonical Notch signaling because neither 20S nor Shh induced CBF1 luciferase reporter activity or NICD protein accumulation in the nucleus, which are hallmarks of canonical Notch signaling activation. Finally, HES‐1 and HEY‐1 siRNA transfection significantly inhibited 20S‐induced osteogenic genes, suggesting that the pro‐osteogenic effects of 20S are regulated in part by HES‐1 and HEY‐1. © 2010 American Society for Bone and Mineral Research</description><subject>Animals</subject><subject>Basic Helix-Loop-Helix Transcription Factors - analysis</subject><subject>Basic Helix-Loop-Helix Transcription Factors - metabolism</subject><subject>Biological and medical sciences</subject><subject>Bone Marrow Cells - drug effects</subject><subject>Bone Marrow Cells - metabolism</subject><subject>Cell Culture Techniques</subject><subject>Cell Cycle Proteins - analysis</subject><subject>Cell Cycle Proteins - metabolism</subject><subject>Fibroblasts - drug effects</subject><subject>Fibroblasts - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression - drug effects</subject><subject>hedgehog</subject><subject>Hedgehog Proteins - metabolism</subject><subject>Homeodomain Proteins - analysis</subject><subject>Homeodomain Proteins - metabolism</subject><subject>Hydroxycholesterols - pharmacology</subject><subject>Liver X Receptors</subject><subject>mesenchymal stem cells</subject><subject>Mice</subject><subject>Notch</subject><subject>Original</subject><subject>Orphan Nuclear Receptors - analysis</subject><subject>Orphan Nuclear Receptors - metabolism</subject><subject>osteogenesis</subject><subject>Osteogenesis - drug effects</subject><subject>oxysterol</subject><subject>Receptors, Notch - genetics</subject><subject>RNA, Messenger - analysis</subject><subject>RNA, Messenger - metabolism</subject><subject>Skeleton and joints</subject><subject>Stromal Cells - drug effects</subject><subject>Stromal Cells - metabolism</subject><subject>Transcription Factor HES-1</subject><subject>Veratrum Alkaloids - pharmacology</subject><subject>Vertebrates: osteoarticular system, musculoskeletal system</subject><issn>0884-0431</issn><issn>1523-4681</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kctu1DAUhi0EokNhxR5FQhVFkOJ7nA0SVFxVVInL2nKc4xmPkniwE9rZ8Qg8I0-CRxnKZdHV0Tnn038uP0L3CT4hTNTP1k0fT3BNMOU30IIIykouFbmJFlgpXmLOyAG6k9IaYyyFlLfRAakVq6tKLlA6TyOEJQzeFuFym5MYuqcFxcefHv_8_mO1bWMu21Xo4HfPD-1kIRVDGO2qGE1cwlhkBSjgchMhJR-GDBVNyKXexBguijTG0JuusNB16S665UyX4N4-HqIvr199Pn1bnp2_eXf64qy0klBeAjHWCMwB20bUhOeEKEeksK4G17bccVDQAEhpratIRSvhamWkYIK6tmKH6Pmsu5maHloLwxhNpzfR5622Ohiv_-0MfqWX4ZtmRDDGaBZ4tBeI4euUH6B7n3YnmAHClHTFRU0V57tRx9eSRFVUMSWFyujD_9B1mOKQH5EpKQUhhOJMPZkpG0NKEdzV2gTrne16Z7uebc_0g78v_cPufc7A0R4wyZrORTNYn644ShUV-YGZozN34TvYXjdTv3_54eM8_Rewp8jk</recordid><startdate>201004</startdate><enddate>201004</enddate><creator>Kim, Woo‐Kyun</creator><creator>Meliton, Vicente</creator><creator>Tetradis, Sotirios</creator><creator>Weinmaster, Gerry</creator><creator>Hahn, Theodore J</creator><creator>Carlson, Marc</creator><creator>Nelson, Stanley F</creator><creator>Parhami, Farhad</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley</general><general>Wiley Subscription Services, Inc</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7TS</scope><scope>K9.</scope><scope>5PM</scope></search><sort><creationdate>201004</creationdate><title>Osteogenic oxysterol, 20(S)‐hydroxycholesterol, induces notch target gene expression in bone marrow stromal cells</title><author>Kim, Woo‐Kyun ; Meliton, Vicente ; Tetradis, Sotirios ; Weinmaster, Gerry ; Hahn, Theodore J ; Carlson, Marc ; Nelson, Stanley F ; Parhami, Farhad</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c6124-e1aca504e0cb5914ca518f165cf9efdd4f4e8ebee66ccf717275f98a65352fd73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Animals</topic><topic>Basic Helix-Loop-Helix Transcription Factors - analysis</topic><topic>Basic Helix-Loop-Helix Transcription Factors - metabolism</topic><topic>Biological and medical sciences</topic><topic>Bone Marrow Cells - drug effects</topic><topic>Bone Marrow Cells - metabolism</topic><topic>Cell Culture Techniques</topic><topic>Cell Cycle Proteins - analysis</topic><topic>Cell Cycle Proteins - metabolism</topic><topic>Fibroblasts - drug effects</topic><topic>Fibroblasts - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression - drug effects</topic><topic>hedgehog</topic><topic>Hedgehog Proteins - metabolism</topic><topic>Homeodomain Proteins - analysis</topic><topic>Homeodomain Proteins - metabolism</topic><topic>Hydroxycholesterols - pharmacology</topic><topic>Liver X Receptors</topic><topic>mesenchymal stem cells</topic><topic>Mice</topic><topic>Notch</topic><topic>Original</topic><topic>Orphan Nuclear Receptors - analysis</topic><topic>Orphan Nuclear Receptors - metabolism</topic><topic>osteogenesis</topic><topic>Osteogenesis - drug effects</topic><topic>oxysterol</topic><topic>Receptors, Notch - genetics</topic><topic>RNA, Messenger - analysis</topic><topic>RNA, Messenger - metabolism</topic><topic>Skeleton and joints</topic><topic>Stromal Cells - drug effects</topic><topic>Stromal Cells - metabolism</topic><topic>Transcription Factor HES-1</topic><topic>Veratrum Alkaloids - pharmacology</topic><topic>Vertebrates: osteoarticular system, musculoskeletal system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kim, Woo‐Kyun</creatorcontrib><creatorcontrib>Meliton, Vicente</creatorcontrib><creatorcontrib>Tetradis, Sotirios</creatorcontrib><creatorcontrib>Weinmaster, Gerry</creatorcontrib><creatorcontrib>Hahn, Theodore J</creatorcontrib><creatorcontrib>Carlson, Marc</creatorcontrib><creatorcontrib>Nelson, Stanley F</creatorcontrib><creatorcontrib>Parhami, Farhad</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Physical Education Index</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of bone and mineral research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kim, Woo‐Kyun</au><au>Meliton, Vicente</au><au>Tetradis, Sotirios</au><au>Weinmaster, Gerry</au><au>Hahn, Theodore J</au><au>Carlson, Marc</au><au>Nelson, Stanley F</au><au>Parhami, Farhad</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Osteogenic oxysterol, 20(S)‐hydroxycholesterol, induces notch target gene expression in bone marrow stromal cells</atitle><jtitle>Journal of bone and mineral research</jtitle><addtitle>J Bone Miner Res</addtitle><date>2010-04</date><risdate>2010</risdate><volume>25</volume><issue>4</issue><spage>782</spage><epage>795</epage><pages>782-795</pages><issn>0884-0431</issn><eissn>1523-4681</eissn><coden>JBMREJ</coden><abstract>We previously reported that specific oxysterols stimulate osteogenic differentiation of pluripotent bone marrow stromal cells (MSCs) through activation of hedgehog (Hh) signaling and may serve as potential future therapies for intervention in osteopenia and osteoporosis. In this study we report that the osteogenic oxysterol 20(S)‐hydroxycholesterol (20S) induces the expression of genes associated with Notch signaling. Using M2‐10B4 (M2) MSCs, we found that 20S significantly induced HES‐1, HEY‐1, and HEY‐2 mRNA expression compared with untreated cells, with maximal induction after 48 hours, whereas the nonosteogenic oxysterols did not. Similar observations were made when M2 cells were treated with sonic hedgehog (Shh), and the specific Hh pathway inhibitor cyclopamine blocked 20S‐induced Notch target gene expression. 20S did not induce Notch target genes in Smo−/− mouse embryonic fibroblasts, further confirming the role of Hh signaling in 20S‐induced expression of Notch target genes. Despite the inability of liver X‐receptor (LXR) synthetic ligand TO901317 to induce Notch target genes in M2 cells, LXR knockdown studies using siRNA showed inhibition of 20S‐induced HEY‐1 but not HES‐1 expression, suggesting the partial role of LXR signaling in MSC responses to 20S. Moreover, 20S‐induced Notch target gene expression was independent of canonical Notch signaling because neither 20S nor Shh induced CBF1 luciferase reporter activity or NICD protein accumulation in the nucleus, which are hallmarks of canonical Notch signaling activation. Finally, HES‐1 and HEY‐1 siRNA transfection significantly inhibited 20S‐induced osteogenic genes, suggesting that the pro‐osteogenic effects of 20S are regulated in part by HES‐1 and HEY‐1. © 2010 American Society for Bone and Mineral Research</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>19839776</pmid><doi>10.1359/jbmr.091024</doi><tpages>14</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0884-0431 |
| ispartof | Journal of bone and mineral research, 2010-04, Vol.25 (4), p.782-795 |
| issn | 0884-0431 1523-4681 |
| language | eng |
| recordid | cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3153332 |
| source | MEDLINE; Access via Wiley Online Library; Oxford University Press Journals All Titles (1996-Current); EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Animals Basic Helix-Loop-Helix Transcription Factors - analysis Basic Helix-Loop-Helix Transcription Factors - metabolism Biological and medical sciences Bone Marrow Cells - drug effects Bone Marrow Cells - metabolism Cell Culture Techniques Cell Cycle Proteins - analysis Cell Cycle Proteins - metabolism Fibroblasts - drug effects Fibroblasts - metabolism Fundamental and applied biological sciences. Psychology Gene Expression - drug effects hedgehog Hedgehog Proteins - metabolism Homeodomain Proteins - analysis Homeodomain Proteins - metabolism Hydroxycholesterols - pharmacology Liver X Receptors mesenchymal stem cells Mice Notch Original Orphan Nuclear Receptors - analysis Orphan Nuclear Receptors - metabolism osteogenesis Osteogenesis - drug effects oxysterol Receptors, Notch - genetics RNA, Messenger - analysis RNA, Messenger - metabolism Skeleton and joints Stromal Cells - drug effects Stromal Cells - metabolism Transcription Factor HES-1 Veratrum Alkaloids - pharmacology Vertebrates: osteoarticular system, musculoskeletal system |
| title | Osteogenic oxysterol, 20(S)‐hydroxycholesterol, induces notch target gene expression in bone marrow stromal cells |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-14T01%3A23%3A11IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Osteogenic%20oxysterol,%2020(S)%E2%80%90hydroxycholesterol,%20induces%20notch%20target%20gene%20expression%20in%20bone%20marrow%20stromal%20cells&rft.jtitle=Journal%20of%20bone%20and%20mineral%20research&rft.au=Kim,%20Woo%E2%80%90Kyun&rft.date=2010-04&rft.volume=25&rft.issue=4&rft.spage=782&rft.epage=795&rft.pages=782-795&rft.issn=0884-0431&rft.eissn=1523-4681&rft.coden=JBMREJ&rft_id=info:doi/10.1359/jbmr.091024&rft_dat=%3Cproquest_pubme%3E745928447%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1866511120&rft_id=info:pmid/19839776&rfr_iscdi=true |