Deep and Highly Sensitive Proteome Coverage by LC-MS/MS Without Prefractionation

In-depth MS-based proteomics has necessitated fractionation of either proteins or peptides or both, often requiring considerable analysis time. Here we employ long liquid chromatography runs with high resolution coupled to an instrument with fast sequencing speed to investigate how much of the prote...

Ausführliche Beschreibung

Bibliographische Detailangaben

Veröffentlicht in:	Molecular & cellular proteomics 2011-08, Vol.10 (8), p.M110.003699-M110.003699, Article M110.003699
Hauptverfasser:	Thakur, Suman S., Geiger, Tamar, Chatterjee, Bhaswati, Bandilla, Peter, Fröhlich, Florian, Cox, Juergen, Mann, Matthias
Format:	Artikel
Sprache:	eng
Schlagworte:	Cell Culture Techniques Cell Fractionation Chromatography, High Pressure Liquid HEK293 Cells Humans Isotope Labeling Limit of Detection Metabolic Networks and Pathways Proteome - isolation & purification Proteome - metabolism Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins - isolation & purification Saccharomyces cerevisiae Proteins - metabolism Tandem Mass Spectrometry Technological Innovation and Resources
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!