An Experimental Model of Meningoencephalomyelitis by Rocio Flavivirus in Balb/C Mice: Inflammatory Response, Cytokine Production, and Histopathology
Rocio virus (ROCV) is a flavivirus, probably transmitted by Culex mosquitoes and maintained in nature as a zoonosis of wild birds. Rocio virus caused a human epidemic of severe encephalitis that lasted from 1973 to 1980 in the Ribeira valley, in the southeastern coast of Brazil. After this outbreak,...
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| Veröffentlicht in: | The American journal of tropical medicine and hygiene 2011-08, Vol.85 (2), p.363-373 |
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| creator | DIAS DE BARROS, Veridiana Ester SAGGIORO, Fabiano P NEDER, Luciano DE OLIVEIRA FRANCA, Rafael Freitas MARIGUELA, Viviane HELENA CHAVEZ, Juliana PENHARVEL, Sandra FORJAZ, Jorge LOPES DA FONSECA, Benedito Antônio MORAES FIGUEIREDO, Luiz Tadeu |
| description | Rocio virus (ROCV) is a flavivirus, probably transmitted by Culex mosquitoes and maintained in nature as a zoonosis of wild birds. Rocio virus caused a human epidemic of severe encephalitis that lasted from 1973 to 1980 in the Ribeira valley, in the southeastern coast of Brazil. After this outbreak, serologic evidence of ROCV circulation has been reported and public health authorities are concerned about a return of ROCV outbreaks in Brazil. We show here a study on the pathogenesis and the physiopathology of ROCV disease in the central nervous system of a Balb/C young adult mice experimental model. The animals were intraperitoneally infected by ROCV and followed from 0 to 9 days after infection, when all of them died. Nervous tissue samples were collected from infected animals for immunohistochemistry and molecular biology analysis. We observed the virus in the central nervous system, the inflammatory changes induced by Th1 and Th2 cytokines, and the final irreversible damage of nervous tissues by neuronal degeneration and apoptosis. These findings can help to better understand the pathogenesis and physiopathology of the human meningoencephalomyelitis by ROCV and other flaviviruses. |
| doi_str_mv | 10.4269/ajtmh.2011.10-0246 |
| format | Article |
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Rocio virus caused a human epidemic of severe encephalitis that lasted from 1973 to 1980 in the Ribeira valley, in the southeastern coast of Brazil. After this outbreak, serologic evidence of ROCV circulation has been reported and public health authorities are concerned about a return of ROCV outbreaks in Brazil. We show here a study on the pathogenesis and the physiopathology of ROCV disease in the central nervous system of a Balb/C young adult mice experimental model. The animals were intraperitoneally infected by ROCV and followed from 0 to 9 days after infection, when all of them died. Nervous tissue samples were collected from infected animals for immunohistochemistry and molecular biology analysis. We observed the virus in the central nervous system, the inflammatory changes induced by Th1 and Th2 cytokines, and the final irreversible damage of nervous tissues by neuronal degeneration and apoptosis. These findings can help to better understand the pathogenesis and physiopathology of the human meningoencephalomyelitis by ROCV and other flaviviruses.</description><identifier>ISSN: 0002-9637</identifier><identifier>EISSN: 1476-1645</identifier><identifier>DOI: 10.4269/ajtmh.2011.10-0246</identifier><identifier>PMID: 21813860</identifier><identifier>CODEN: AJTHAB</identifier><language>eng</language><publisher>Deerfield, IL: American Society of Tropical Medecine and Hygiene</publisher><subject>Animals ; Biological and medical sciences ; Brain - cytology ; Culex ; Cytokines - genetics ; Cytokines - metabolism ; Disease Models, Animal ; Encephalomyelitis - pathology ; Flaviviridae - classification ; Flaviviridae Infections - metabolism ; Flaviviridae Infections - pathology ; Flaviviridae Infections - virology ; Flavivirus ; Gene Expression Regulation - physiology ; Infectious diseases ; Inflammation - virology ; Lymphocytes - physiology ; Medical sciences ; Mice ; Mice, Inbred BALB C ; Neutrophils - physiology ; Polymerase Chain Reaction - methods ; RNA, Viral ; Rocio virus ; Spinal Cord - cytology</subject><ispartof>The American journal of tropical medicine and hygiene, 2011-08, Vol.85 (2), p.363-373</ispartof><rights>2015 INIST-CNRS</rights><rights>The American Society of Tropical Medicine and Hygiene 2011</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c464t-3ebe80743e277df30e7ef3f5c1d51c0ee7720bc351c5712849a836a067a794833</citedby><cites>FETCH-LOGICAL-c464t-3ebe80743e277df30e7ef3f5c1d51c0ee7720bc351c5712849a836a067a794833</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3144838/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3144838/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,728,781,785,886,27925,27926,53792,53794</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=24424613$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21813860$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>DIAS DE BARROS, Veridiana Ester</creatorcontrib><creatorcontrib>SAGGIORO, Fabiano P</creatorcontrib><creatorcontrib>NEDER, Luciano</creatorcontrib><creatorcontrib>DE OLIVEIRA FRANCA, Rafael Freitas</creatorcontrib><creatorcontrib>MARIGUELA, Viviane</creatorcontrib><creatorcontrib>HELENA CHAVEZ, Juliana</creatorcontrib><creatorcontrib>PENHARVEL, Sandra</creatorcontrib><creatorcontrib>FORJAZ, Jorge</creatorcontrib><creatorcontrib>LOPES DA FONSECA, Benedito Antônio</creatorcontrib><creatorcontrib>MORAES FIGUEIREDO, Luiz Tadeu</creatorcontrib><title>An Experimental Model of Meningoencephalomyelitis by Rocio Flavivirus in Balb/C Mice: Inflammatory Response, Cytokine Production, and Histopathology</title><title>The American journal of tropical medicine and hygiene</title><addtitle>Am J Trop Med Hyg</addtitle><description>Rocio virus (ROCV) is a flavivirus, probably transmitted by Culex mosquitoes and maintained in nature as a zoonosis of wild birds. 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Rocio virus caused a human epidemic of severe encephalitis that lasted from 1973 to 1980 in the Ribeira valley, in the southeastern coast of Brazil. After this outbreak, serologic evidence of ROCV circulation has been reported and public health authorities are concerned about a return of ROCV outbreaks in Brazil. We show here a study on the pathogenesis and the physiopathology of ROCV disease in the central nervous system of a Balb/C young adult mice experimental model. The animals were intraperitoneally infected by ROCV and followed from 0 to 9 days after infection, when all of them died. Nervous tissue samples were collected from infected animals for immunohistochemistry and molecular biology analysis. We observed the virus in the central nervous system, the inflammatory changes induced by Th1 and Th2 cytokines, and the final irreversible damage of nervous tissues by neuronal degeneration and apoptosis. These findings can help to better understand the pathogenesis and physiopathology of the human meningoencephalomyelitis by ROCV and other flaviviruses.</abstract><cop>Deerfield, IL</cop><pub>American Society of Tropical Medecine and Hygiene</pub><pmid>21813860</pmid><doi>10.4269/ajtmh.2011.10-0246</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Alma/SFX Local Collection |
| subjects | Animals Biological and medical sciences Brain - cytology Culex Cytokines - genetics Cytokines - metabolism Disease Models, Animal Encephalomyelitis - pathology Flaviviridae - classification Flaviviridae Infections - metabolism Flaviviridae Infections - pathology Flaviviridae Infections - virology Flavivirus Gene Expression Regulation - physiology Infectious diseases Inflammation - virology Lymphocytes - physiology Medical sciences Mice Mice, Inbred BALB C Neutrophils - physiology Polymerase Chain Reaction - methods RNA, Viral Rocio virus Spinal Cord - cytology |
| title | An Experimental Model of Meningoencephalomyelitis by Rocio Flavivirus in Balb/C Mice: Inflammatory Response, Cytokine Production, and Histopathology |
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