Microgenomics of Ameloblastoma
Gene expression profiles of human ameloblastoma microdissected cells were characterized with the purpose of identifying genes and their protein products that could be targeted as diagnostic and prognostic markers as well as for potential therapeutic interventions. Five formalin-fixed, decalcified, p...
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| Veröffentlicht in: | Journal of dental research 2011-04, Vol.90 (4), p.463-469 |
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| creator | DeVilliers, P. Suggs, C. Simmons, D. Murrah, V. Wright, J.T. |
| description | Gene expression profiles of human ameloblastoma microdissected cells were characterized with the purpose of identifying genes and their protein products that could be targeted as diagnostic and prognostic markers as well as for potential therapeutic interventions. Five formalin-fixed, decalcified, paraffin-embedded samples of ameloblastoma were subjected to laser capture microdissection, linear mRNA amplification, and hybridization to oligonucleotide human 41,000 RNA arrays and compared with universal human reference RNA, to determine the gene expression signature. Assessment of the data by Significance Analysis of Microarrays (SAM) and cluster analysis showed that 38 genes were highly expressed (two-fold increase) in all samples, while 41 genes were underexpressed (two-fold reduction). Elements of the sonic hedgehog pathway and Wingless type MMTV integration site family were validated by immunohistochemistry. We have identified the expression of multiple genes and protein products that could serve as potential diagnostic, prognostic, and therapeutic targets. |
| doi_str_mv | 10.1177/0022034510391791 |
| format | Article |
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Five formalin-fixed, decalcified, paraffin-embedded samples of ameloblastoma were subjected to laser capture microdissection, linear mRNA amplification, and hybridization to oligonucleotide human 41,000 RNA arrays and compared with universal human reference RNA, to determine the gene expression signature. Assessment of the data by Significance Analysis of Microarrays (SAM) and cluster analysis showed that 38 genes were highly expressed (two-fold increase) in all samples, while 41 genes were underexpressed (two-fold reduction). Elements of the sonic hedgehog pathway and Wingless type MMTV integration site family were validated by immunohistochemistry. We have identified the expression of multiple genes and protein products that could serve as potential diagnostic, prognostic, and therapeutic targets.</description><identifier>ISSN: 0022-0345</identifier><identifier>EISSN: 1544-0591</identifier><identifier>DOI: 10.1177/0022034510391791</identifier><identifier>PMID: 21282726</identifier><language>eng</language><publisher>Los Angeles, CA: SAGE Publications</publisher><subject>Ameloblastoma ; Ameloblastoma - genetics ; Amelogenin - genetics ; Archives & records ; Biological and medical sciences ; Biomarkers, Tumor - genetics ; Calbindin 2 ; Cell growth ; Dental Enamel Proteins - genetics ; Dentistry ; DNA microarrays ; Extracellular Matrix Proteins ; Facial bones, jaws, teeth, parodontium: diseases, semeiology ; Gene expression ; Gene Expression Profiling - methods ; Gene therapy ; Genomics - methods ; Hedgehog Proteins - genetics ; Humans ; Hybridization ; Immunohistochemistry ; Kallikreins - genetics ; Lasers ; Lasers, Semiconductor ; Matrix Metalloproteinase 20 - genetics ; Medical sciences ; Metastasis ; Microdissection - methods ; Morphology ; Neoplasm Proteins - genetics ; Nucleic Acid Hybridization ; Oligonucleotide Array Sequence Analysis ; Oligonucleotides ; Otorhinolaryngology. Stomatology ; Paraffin ; Patched Receptors ; Pathology ; Proteins ; Proteins - genetics ; Receptors, Cell Surface - genetics ; Receptors, G-Protein-Coupled - genetics ; Research Reports ; RNA, Messenger - genetics ; RNA, Neoplasm - genetics ; S100 Calcium Binding Protein G - genetics ; Signal transduction ; Smoothened Receptor ; Studies ; Therapeutic applications ; Tumor necrosis factor-TNF ; Tumorigenesis ; Tumors ; Wnt Proteins - genetics</subject><ispartof>Journal of dental research, 2011-04, Vol.90 (4), p.463-469</ispartof><rights>2011 International & American Associations for Dental Research</rights><rights>2015 INIST-CNRS</rights><rights>2011 International & American Associations for Dental Research 2011 International & American Associations for Dental Research</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c586t-bad9b0d5a00cd4c088965a658b2717b59fe112b0d86478ab3dac3fcfda06bc6c3</citedby><cites>FETCH-LOGICAL-c586t-bad9b0d5a00cd4c088965a658b2717b59fe112b0d86478ab3dac3fcfda06bc6c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://journals.sagepub.com/doi/pdf/10.1177/0022034510391791$$EPDF$$P50$$Gsage$$H</linktopdf><linktohtml>$$Uhttps://journals.sagepub.com/doi/10.1177/0022034510391791$$EHTML$$P50$$Gsage$$H</linktohtml><link.rule.ids>230,314,780,784,885,21819,27924,27925,43621,43622</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=24158157$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21282726$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>DeVilliers, P.</creatorcontrib><creatorcontrib>Suggs, C.</creatorcontrib><creatorcontrib>Simmons, D.</creatorcontrib><creatorcontrib>Murrah, V.</creatorcontrib><creatorcontrib>Wright, J.T.</creatorcontrib><title>Microgenomics of Ameloblastoma</title><title>Journal of dental research</title><addtitle>J Dent Res</addtitle><description>Gene expression profiles of human ameloblastoma microdissected cells were characterized with the purpose of identifying genes and their protein products that could be targeted as diagnostic and prognostic markers as well as for potential therapeutic interventions. Five formalin-fixed, decalcified, paraffin-embedded samples of ameloblastoma were subjected to laser capture microdissection, linear mRNA amplification, and hybridization to oligonucleotide human 41,000 RNA arrays and compared with universal human reference RNA, to determine the gene expression signature. Assessment of the data by Significance Analysis of Microarrays (SAM) and cluster analysis showed that 38 genes were highly expressed (two-fold increase) in all samples, while 41 genes were underexpressed (two-fold reduction). Elements of the sonic hedgehog pathway and Wingless type MMTV integration site family were validated by immunohistochemistry. We have identified the expression of multiple genes and protein products that could serve as potential diagnostic, prognostic, and therapeutic targets.</description><subject>Ameloblastoma</subject><subject>Ameloblastoma - genetics</subject><subject>Amelogenin - genetics</subject><subject>Archives & records</subject><subject>Biological and medical sciences</subject><subject>Biomarkers, Tumor - genetics</subject><subject>Calbindin 2</subject><subject>Cell growth</subject><subject>Dental Enamel Proteins - genetics</subject><subject>Dentistry</subject><subject>DNA microarrays</subject><subject>Extracellular Matrix Proteins</subject><subject>Facial bones, jaws, teeth, parodontium: diseases, semeiology</subject><subject>Gene expression</subject><subject>Gene Expression Profiling - methods</subject><subject>Gene therapy</subject><subject>Genomics - methods</subject><subject>Hedgehog Proteins - genetics</subject><subject>Humans</subject><subject>Hybridization</subject><subject>Immunohistochemistry</subject><subject>Kallikreins - genetics</subject><subject>Lasers</subject><subject>Lasers, Semiconductor</subject><subject>Matrix Metalloproteinase 20 - genetics</subject><subject>Medical sciences</subject><subject>Metastasis</subject><subject>Microdissection - methods</subject><subject>Morphology</subject><subject>Neoplasm Proteins - genetics</subject><subject>Nucleic Acid Hybridization</subject><subject>Oligonucleotide Array Sequence Analysis</subject><subject>Oligonucleotides</subject><subject>Otorhinolaryngology. Stomatology</subject><subject>Paraffin</subject><subject>Patched Receptors</subject><subject>Pathology</subject><subject>Proteins</subject><subject>Proteins - genetics</subject><subject>Receptors, Cell Surface - genetics</subject><subject>Receptors, G-Protein-Coupled - genetics</subject><subject>Research Reports</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Neoplasm - genetics</subject><subject>S100 Calcium Binding Protein G - genetics</subject><subject>Signal transduction</subject><subject>Smoothened Receptor</subject><subject>Studies</subject><subject>Therapeutic applications</subject><subject>Tumor necrosis factor-TNF</subject><subject>Tumorigenesis</subject><subject>Tumors</subject><subject>Wnt Proteins - genetics</subject><issn>0022-0345</issn><issn>1544-0591</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kM1LAzEQxYMotlbvnkQQ8bQ6k2w2yUUQ8QsUL3oOs9lsXdnd1E0r-N-7pbXVgqc5vN-8mfcYO0Q4R1TqAoBzEKlEEAaVwS02RJmmCUiD22w4l5O5PmB7Mb4DoOFa7LIBR6654tmQHT1Vrgtj34amcvE4lMdXja9DXlOchob22U5JdfQHyzlir7c3L9f3yePz3cP11WPipM6mSU6FyaGQBOCK1IHWJpOUSZ1zhSqXpvSIvCd0lipNuSjIidKVBUGWu8yJEbtc-E5meeML59tpR7WddFVD3ZcNVNm_Slu92XH4tALTFIXsDc6WBl34mPk4tU0Vna9ran2YRaulAakVNz15skG-h1nX9uksFwAZatBzChZU306MnS9XvyDYefd2s_t-5eh3htXCT9k9cLoEKDqqy45aV8U1l6LUKFXPJQsu0tivv_v38DfvO5eU</recordid><startdate>20110401</startdate><enddate>20110401</enddate><creator>DeVilliers, P.</creator><creator>Suggs, C.</creator><creator>Simmons, D.</creator><creator>Murrah, V.</creator><creator>Wright, J.T.</creator><general>SAGE Publications</general><general>International Association for Dental Research</general><general>SAGE PUBLICATIONS, INC</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>NAPCQ</scope><scope>U9A</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20110401</creationdate><title>Microgenomics of Ameloblastoma</title><author>DeVilliers, P. ; Suggs, C. ; Simmons, D. ; Murrah, V. ; Wright, J.T.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c586t-bad9b0d5a00cd4c088965a658b2717b59fe112b0d86478ab3dac3fcfda06bc6c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Ameloblastoma</topic><topic>Ameloblastoma - genetics</topic><topic>Amelogenin - genetics</topic><topic>Archives & records</topic><topic>Biological and medical sciences</topic><topic>Biomarkers, Tumor - genetics</topic><topic>Calbindin 2</topic><topic>Cell growth</topic><topic>Dental Enamel Proteins - genetics</topic><topic>Dentistry</topic><topic>DNA microarrays</topic><topic>Extracellular Matrix Proteins</topic><topic>Facial bones, jaws, teeth, parodontium: diseases, semeiology</topic><topic>Gene expression</topic><topic>Gene Expression Profiling - methods</topic><topic>Gene therapy</topic><topic>Genomics - methods</topic><topic>Hedgehog Proteins - genetics</topic><topic>Humans</topic><topic>Hybridization</topic><topic>Immunohistochemistry</topic><topic>Kallikreins - genetics</topic><topic>Lasers</topic><topic>Lasers, Semiconductor</topic><topic>Matrix Metalloproteinase 20 - genetics</topic><topic>Medical sciences</topic><topic>Metastasis</topic><topic>Microdissection - methods</topic><topic>Morphology</topic><topic>Neoplasm Proteins - genetics</topic><topic>Nucleic Acid Hybridization</topic><topic>Oligonucleotide Array Sequence Analysis</topic><topic>Oligonucleotides</topic><topic>Otorhinolaryngology. Stomatology</topic><topic>Paraffin</topic><topic>Patched Receptors</topic><topic>Pathology</topic><topic>Proteins</topic><topic>Proteins - genetics</topic><topic>Receptors, Cell Surface - genetics</topic><topic>Receptors, G-Protein-Coupled - genetics</topic><topic>Research Reports</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Neoplasm - genetics</topic><topic>S100 Calcium Binding Protein G - genetics</topic><topic>Signal transduction</topic><topic>Smoothened Receptor</topic><topic>Studies</topic><topic>Therapeutic applications</topic><topic>Tumor necrosis factor-TNF</topic><topic>Tumorigenesis</topic><topic>Tumors</topic><topic>Wnt Proteins - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>DeVilliers, P.</creatorcontrib><creatorcontrib>Suggs, C.</creatorcontrib><creatorcontrib>Simmons, D.</creatorcontrib><creatorcontrib>Murrah, V.</creatorcontrib><creatorcontrib>Wright, J.T.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Premium</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of dental research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>DeVilliers, P.</au><au>Suggs, C.</au><au>Simmons, D.</au><au>Murrah, V.</au><au>Wright, J.T.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Microgenomics of Ameloblastoma</atitle><jtitle>Journal of dental research</jtitle><addtitle>J Dent Res</addtitle><date>2011-04-01</date><risdate>2011</risdate><volume>90</volume><issue>4</issue><spage>463</spage><epage>469</epage><pages>463-469</pages><issn>0022-0345</issn><eissn>1544-0591</eissn><abstract>Gene expression profiles of human ameloblastoma microdissected cells were characterized with the purpose of identifying genes and their protein products that could be targeted as diagnostic and prognostic markers as well as for potential therapeutic interventions. Five formalin-fixed, decalcified, paraffin-embedded samples of ameloblastoma were subjected to laser capture microdissection, linear mRNA amplification, and hybridization to oligonucleotide human 41,000 RNA arrays and compared with universal human reference RNA, to determine the gene expression signature. Assessment of the data by Significance Analysis of Microarrays (SAM) and cluster analysis showed that 38 genes were highly expressed (two-fold increase) in all samples, while 41 genes were underexpressed (two-fold reduction). Elements of the sonic hedgehog pathway and Wingless type MMTV integration site family were validated by immunohistochemistry. We have identified the expression of multiple genes and protein products that could serve as potential diagnostic, prognostic, and therapeutic targets.</abstract><cop>Los Angeles, CA</cop><pub>SAGE Publications</pub><pmid>21282726</pmid><doi>10.1177/0022034510391791</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Ameloblastoma Ameloblastoma - genetics Amelogenin - genetics Archives & records Biological and medical sciences Biomarkers, Tumor - genetics Calbindin 2 Cell growth Dental Enamel Proteins - genetics Dentistry DNA microarrays Extracellular Matrix Proteins Facial bones, jaws, teeth, parodontium: diseases, semeiology Gene expression Gene Expression Profiling - methods Gene therapy Genomics - methods Hedgehog Proteins - genetics Humans Hybridization Immunohistochemistry Kallikreins - genetics Lasers Lasers, Semiconductor Matrix Metalloproteinase 20 - genetics Medical sciences Metastasis Microdissection - methods Morphology Neoplasm Proteins - genetics Nucleic Acid Hybridization Oligonucleotide Array Sequence Analysis Oligonucleotides Otorhinolaryngology. Stomatology Paraffin Patched Receptors Pathology Proteins Proteins - genetics Receptors, Cell Surface - genetics Receptors, G-Protein-Coupled - genetics Research Reports RNA, Messenger - genetics RNA, Neoplasm - genetics S100 Calcium Binding Protein G - genetics Signal transduction Smoothened Receptor Studies Therapeutic applications Tumor necrosis factor-TNF Tumorigenesis Tumors Wnt Proteins - genetics |
| title | Microgenomics of Ameloblastoma |
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