Proteases of an Early Colonizer Can Hinder Streptococcus mutans Colonization in vitro
Streptococcus mutans is the primary cariogen that produces several virulence factors that are modulated by a competence-stimulating peptide (CSP) signaling system. In this study, we sought to determine if proteases produced by early dental plaque colonizers such as Streptococcus gordonii interfere w...
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| Veröffentlicht in: | Journal of dental research 2011-04, Vol.90 (4), p.501-505 |
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| creator | Wang, B.-Y. Deutch, A. Hong, J. Kuramitsu, H.K. |
| description | Streptococcus mutans is the primary cariogen that produces several virulence factors that are modulated by a competence-stimulating peptide (CSP) signaling system. In this study, we sought to determine if proteases produced by early dental plaque colonizers such as Streptococcus gordonii interfere with the subsequent colonization of S. mutans BM71 on the existing streptococcal biofilms. We demonstrated that S. mutans BM71 colonized much less efficiently in vitro on streptococcal biofilms than on Actinomyces naeslundii biofilms. Several oral streptococci, relative to A. naeslundii, produced proteases that inactivated the S. mutans CSP. We further demonstrated that cell protein extracts from S. gordonii, but not from A. naeslundii, interfered with S. mutans BM71 colonization. In addition, S. mutans BM71 colonized more efficiently on the sgc protease knockout mutant of S. gordonii than on the parent biofilms. In conclusion, proteases of early colonizers can interfere with subsequent colonization by S. mutans
in vitro. |
| doi_str_mv | 10.1177/0022034510388808 |
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in vitro.</description><identifier>ISSN: 0022-0345</identifier><identifier>EISSN: 1544-0591</identifier><identifier>DOI: 10.1177/0022034510388808</identifier><identifier>PMID: 21088146</identifier><language>eng</language><publisher>Los Angeles, CA: SAGE Publications</publisher><subject>Actinomyces - physiology ; Antibiosis ; Bacteria ; Bacterial Adhesion - drug effects ; Bacterial Proteins - drug effects ; Bacterial Proteins - pharmacology ; Bacteriocins - analysis ; Bacteriological Techniques ; Biofilms ; Biological and medical sciences ; Caseins - drug effects ; Colonization ; Dental caries ; Dental plaque ; Dental Plaque - microbiology ; Dentistry ; Facial bones, jaws, teeth, parodontium: diseases, semeiology ; Gene Knockout Techniques ; Gram-positive bacteria ; Humans ; Laboratory animals ; Medical sciences ; Non tumoral diseases ; Otorhinolaryngology. Stomatology ; Oxidation ; Peptide Hydrolases - pharmacology ; Research Reports ; Serine Proteases - pharmacology ; Signal transduction ; Streptococcus - enzymology ; Streptococcus gordonii - enzymology ; Streptococcus gordonii - genetics ; Streptococcus gordonii - physiology ; Streptococcus infections ; Streptococcus mitis - enzymology ; Streptococcus mutans ; Streptococcus mutans - drug effects ; Streptococcus mutans - physiology ; Streptococcus oralis - enzymology ; Studies ; Virulence factors</subject><ispartof>Journal of dental research, 2011-04, Vol.90 (4), p.501-505</ispartof><rights>2011 International & American Associations for Dental Research</rights><rights>2015 INIST-CNRS</rights><rights>2011 International & American Associations for Dental Research 2011 International & American Associations for Dental Research</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c586t-e7560390486043209a8332323ddbaeb093cd995bc4de563d11e30056cee5d4db3</citedby><cites>FETCH-LOGICAL-c586t-e7560390486043209a8332323ddbaeb093cd995bc4de563d11e30056cee5d4db3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://journals.sagepub.com/doi/pdf/10.1177/0022034510388808$$EPDF$$P50$$Gsage$$H</linktopdf><linktohtml>$$Uhttps://journals.sagepub.com/doi/10.1177/0022034510388808$$EHTML$$P50$$Gsage$$H</linktohtml><link.rule.ids>230,314,776,780,881,21798,27901,27902,43597,43598</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=24158163$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21088146$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, B.-Y.</creatorcontrib><creatorcontrib>Deutch, A.</creatorcontrib><creatorcontrib>Hong, J.</creatorcontrib><creatorcontrib>Kuramitsu, H.K.</creatorcontrib><title>Proteases of an Early Colonizer Can Hinder Streptococcus mutans Colonization in vitro</title><title>Journal of dental research</title><addtitle>J Dent Res</addtitle><description>Streptococcus mutans is the primary cariogen that produces several virulence factors that are modulated by a competence-stimulating peptide (CSP) signaling system. In this study, we sought to determine if proteases produced by early dental plaque colonizers such as Streptococcus gordonii interfere with the subsequent colonization of S. mutans BM71 on the existing streptococcal biofilms. We demonstrated that S. mutans BM71 colonized much less efficiently in vitro on streptococcal biofilms than on Actinomyces naeslundii biofilms. Several oral streptococci, relative to A. naeslundii, produced proteases that inactivated the S. mutans CSP. We further demonstrated that cell protein extracts from S. gordonii, but not from A. naeslundii, interfered with S. mutans BM71 colonization. In addition, S. mutans BM71 colonized more efficiently on the sgc protease knockout mutant of S. gordonii than on the parent biofilms. In conclusion, proteases of early colonizers can interfere with subsequent colonization by S. mutans
in vitro.</description><subject>Actinomyces - physiology</subject><subject>Antibiosis</subject><subject>Bacteria</subject><subject>Bacterial Adhesion - drug effects</subject><subject>Bacterial Proteins - drug effects</subject><subject>Bacterial Proteins - pharmacology</subject><subject>Bacteriocins - analysis</subject><subject>Bacteriological Techniques</subject><subject>Biofilms</subject><subject>Biological and medical sciences</subject><subject>Caseins - drug effects</subject><subject>Colonization</subject><subject>Dental caries</subject><subject>Dental plaque</subject><subject>Dental Plaque - microbiology</subject><subject>Dentistry</subject><subject>Facial bones, jaws, teeth, parodontium: diseases, semeiology</subject><subject>Gene Knockout Techniques</subject><subject>Gram-positive bacteria</subject><subject>Humans</subject><subject>Laboratory animals</subject><subject>Medical sciences</subject><subject>Non tumoral diseases</subject><subject>Otorhinolaryngology. Stomatology</subject><subject>Oxidation</subject><subject>Peptide Hydrolases - pharmacology</subject><subject>Research Reports</subject><subject>Serine Proteases - pharmacology</subject><subject>Signal transduction</subject><subject>Streptococcus - enzymology</subject><subject>Streptococcus gordonii - enzymology</subject><subject>Streptococcus gordonii - genetics</subject><subject>Streptococcus gordonii - physiology</subject><subject>Streptococcus infections</subject><subject>Streptococcus mitis - enzymology</subject><subject>Streptococcus mutans</subject><subject>Streptococcus mutans - drug effects</subject><subject>Streptococcus mutans - physiology</subject><subject>Streptococcus oralis - enzymology</subject><subject>Studies</subject><subject>Virulence factors</subject><issn>0022-0345</issn><issn>1544-0591</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kd1rFDEUxYNY7Lr13ScZEPFp6s3kY5IXQZZqhYKC7XPIJHdrymyyJjOF-tebZbetFiQPCTm_e3NuDiGvKZxS2vcfALoOGBcUmFIK1DOyoILzFoSmz8liJ7c7_Zi8LOUGgOpOsRfkuKOgFOVyQa6-5zShLViatG5sbM5sHu-aVRpTDL8xN6t6dx6ir8cfU8btlFxybi7NZp5sLPeknUKKTYjNbZhyOiFHazsWfHXYl-Tq89nl6ry9-Pbl6-rTReuEklOLvZDANHAlgbMOtFWMdXV5P1gcQDPntRaD4x6FZJ5SZABCOkThuR_Yknzc993Owwa9wzhlO5ptDhub70yywfyrxPDTXKdbwyjntP7Fkrw_NMjp14xlMptQHI6jjZjmYpTQIBSjupJvn5A3ac6xTme6akrSXvY7CvaUy6mUjOsHLxTMLjLzNLJa8ubvGR4K7jOqwLsDYIuz4zrb6EJ55DgVikpWuXbPFXuNj-7--_AfOuWqyQ</recordid><startdate>20110401</startdate><enddate>20110401</enddate><creator>Wang, B.-Y.</creator><creator>Deutch, A.</creator><creator>Hong, J.</creator><creator>Kuramitsu, H.K.</creator><general>SAGE Publications</general><general>International Association for Dental Research</general><general>SAGE PUBLICATIONS, INC</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>NAPCQ</scope><scope>U9A</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20110401</creationdate><title>Proteases of an Early Colonizer Can Hinder Streptococcus mutans Colonization in vitro</title><author>Wang, B.-Y. ; Deutch, A. ; Hong, J. ; Kuramitsu, H.K.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c586t-e7560390486043209a8332323ddbaeb093cd995bc4de563d11e30056cee5d4db3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Actinomyces - physiology</topic><topic>Antibiosis</topic><topic>Bacteria</topic><topic>Bacterial Adhesion - drug effects</topic><topic>Bacterial Proteins - drug effects</topic><topic>Bacterial Proteins - pharmacology</topic><topic>Bacteriocins - analysis</topic><topic>Bacteriological Techniques</topic><topic>Biofilms</topic><topic>Biological and medical sciences</topic><topic>Caseins - drug effects</topic><topic>Colonization</topic><topic>Dental caries</topic><topic>Dental plaque</topic><topic>Dental Plaque - microbiology</topic><topic>Dentistry</topic><topic>Facial bones, jaws, teeth, parodontium: diseases, semeiology</topic><topic>Gene Knockout Techniques</topic><topic>Gram-positive bacteria</topic><topic>Humans</topic><topic>Laboratory animals</topic><topic>Medical sciences</topic><topic>Non tumoral diseases</topic><topic>Otorhinolaryngology. Stomatology</topic><topic>Oxidation</topic><topic>Peptide Hydrolases - pharmacology</topic><topic>Research Reports</topic><topic>Serine Proteases - pharmacology</topic><topic>Signal transduction</topic><topic>Streptococcus - enzymology</topic><topic>Streptococcus gordonii - enzymology</topic><topic>Streptococcus gordonii - genetics</topic><topic>Streptococcus gordonii - physiology</topic><topic>Streptococcus infections</topic><topic>Streptococcus mitis - enzymology</topic><topic>Streptococcus mutans</topic><topic>Streptococcus mutans - drug effects</topic><topic>Streptococcus mutans - physiology</topic><topic>Streptococcus oralis - enzymology</topic><topic>Studies</topic><topic>Virulence factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, B.-Y.</creatorcontrib><creatorcontrib>Deutch, A.</creatorcontrib><creatorcontrib>Hong, J.</creatorcontrib><creatorcontrib>Kuramitsu, H.K.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Premium</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of dental research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, B.-Y.</au><au>Deutch, A.</au><au>Hong, J.</au><au>Kuramitsu, H.K.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Proteases of an Early Colonizer Can Hinder Streptococcus mutans Colonization in vitro</atitle><jtitle>Journal of dental research</jtitle><addtitle>J Dent Res</addtitle><date>2011-04-01</date><risdate>2011</risdate><volume>90</volume><issue>4</issue><spage>501</spage><epage>505</epage><pages>501-505</pages><issn>0022-0345</issn><eissn>1544-0591</eissn><abstract>Streptococcus mutans is the primary cariogen that produces several virulence factors that are modulated by a competence-stimulating peptide (CSP) signaling system. In this study, we sought to determine if proteases produced by early dental plaque colonizers such as Streptococcus gordonii interfere with the subsequent colonization of S. mutans BM71 on the existing streptococcal biofilms. We demonstrated that S. mutans BM71 colonized much less efficiently in vitro on streptococcal biofilms than on Actinomyces naeslundii biofilms. Several oral streptococci, relative to A. naeslundii, produced proteases that inactivated the S. mutans CSP. We further demonstrated that cell protein extracts from S. gordonii, but not from A. naeslundii, interfered with S. mutans BM71 colonization. In addition, S. mutans BM71 colonized more efficiently on the sgc protease knockout mutant of S. gordonii than on the parent biofilms. In conclusion, proteases of early colonizers can interfere with subsequent colonization by S. mutans
in vitro.</abstract><cop>Los Angeles, CA</cop><pub>SAGE Publications</pub><pmid>21088146</pmid><doi>10.1177/0022034510388808</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Actinomyces - physiology Antibiosis Bacteria Bacterial Adhesion - drug effects Bacterial Proteins - drug effects Bacterial Proteins - pharmacology Bacteriocins - analysis Bacteriological Techniques Biofilms Biological and medical sciences Caseins - drug effects Colonization Dental caries Dental plaque Dental Plaque - microbiology Dentistry Facial bones, jaws, teeth, parodontium: diseases, semeiology Gene Knockout Techniques Gram-positive bacteria Humans Laboratory animals Medical sciences Non tumoral diseases Otorhinolaryngology. Stomatology Oxidation Peptide Hydrolases - pharmacology Research Reports Serine Proteases - pharmacology Signal transduction Streptococcus - enzymology Streptococcus gordonii - enzymology Streptococcus gordonii - genetics Streptococcus gordonii - physiology Streptococcus infections Streptococcus mitis - enzymology Streptococcus mutans Streptococcus mutans - drug effects Streptococcus mutans - physiology Streptococcus oralis - enzymology Studies Virulence factors |
| title | Proteases of an Early Colonizer Can Hinder Streptococcus mutans Colonization in vitro |
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