Lectin-mediated microfluidic capture and release of leukemic lymphocytes from whole blood
Lectins are a group of proteins that bind specifically and reversibly to mono- and oligosaccharide carbohydrate structures that are present on the surfaces of mammalian cells. The use of lectins as capture agents in microfluidic channels was examined with a focus on cells associated with T and B lym...
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| Veröffentlicht in: | Biomedical microdevices 2011-06, Vol.13 (3), p.565-571 |
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| creator | Vickers, Dwayne A. L. Hincapie, Marina Hancock, William S. Murthy, Shashi K. |
| description | Lectins are a group of proteins that bind specifically and reversibly to mono- and oligosaccharide carbohydrate structures that are present on the surfaces of mammalian cells. The use of lectins as capture agents in microfluidic channels was examined with a focus on cells associated with T and B lymphocytic leukemia. In addition to examining the adhesion of Jurkat T and Raji B lymphocytes to a broad panel of lectins, this work also examined the capture of these cells from whole blood. Captured T and B lymphocytes were eluted from the microfluidic devices with a solution of the lectin’s inhibiting sugar. The capture and release steps were accomplished in under 1 h. The significance of this work lies within the realm of low-cost capture of abundant target cells with non-stimulatory elution capability. |
| doi_str_mv | 10.1007/s10544-011-9527-5 |
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L.</creatorcontrib><creatorcontrib>Hincapie, Marina</creatorcontrib><creatorcontrib>Hancock, William S.</creatorcontrib><creatorcontrib>Murthy, Shashi K.</creatorcontrib><title>Lectin-mediated microfluidic capture and release of leukemic lymphocytes from whole blood</title><title>Biomedical microdevices</title><addtitle>Biomed Microdevices</addtitle><addtitle>Biomed Microdevices</addtitle><description>Lectins are a group of proteins that bind specifically and reversibly to mono- and oligosaccharide carbohydrate structures that are present on the surfaces of mammalian cells. The use of lectins as capture agents in microfluidic channels was examined with a focus on cells associated with T and B lymphocytic leukemia. In addition to examining the adhesion of Jurkat T and Raji B lymphocytes to a broad panel of lectins, this work also examined the capture of these cells from whole blood. Captured T and B lymphocytes were eluted from the microfluidic devices with a solution of the lectin’s inhibiting sugar. The capture and release steps were accomplished in under 1 h. The significance of this work lies within the realm of low-cost capture of abundant target cells with non-stimulatory elution capability.</description><subject>B-Lymphocytes - cytology</subject><subject>B-Lymphocytes - metabolism</subject><subject>Biological and Medical Physics</subject><subject>Biomedical engineering</subject><subject>Biomedical Engineering and Bioengineering</subject><subject>Biophysics</subject><subject>Blood</subject><subject>Cell Adhesion</subject><subject>Cell adhesion & migration</subject><subject>Cell Separation - instrumentation</subject><subject>Engineering</subject><subject>Engineering Fluid Dynamics</subject><subject>Fluid dynamics</subject><subject>Humans</subject><subject>Immobilized Proteins - chemistry</subject><subject>Immobilized Proteins - metabolism</subject><subject>Jurkat Cells</subject><subject>Leukemia</subject><subject>Leukemia - blood</subject><subject>Lymphocytes</subject><subject>Microelectromechanical systems</subject><subject>Microfluidic Analytical Techniques - instrumentation</subject><subject>Nanotechnology</subject><subject>Plant Lectins - chemistry</subject><subject>Plant Lectins - metabolism</subject><subject>T-Lymphocytes - cytology</subject><subject>T-Lymphocytes - metabolism</subject><issn>1387-2176</issn><issn>1572-8781</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNp1kUuLFDEUhYMozjj6A9xIcOOqNDfvbAQZfEGDG124CunUrekaU5U2qRrpf2-aHscHuMol97snOfcQ8hTYS2DMvKrAlJQdA-ic4qZT98g5KMM7ayzcb7WwpuNg9Bl5VOs1Y-C01g_JGQeplFH6nHzdYFzGuZuwH8OCPZ3GWPKQ1rEfI41hv6wFaZh7WjBhqEjzQBOu37CBNB2m_S7Hw4KVDiVP9McuJ6TblHP_mDwYQqr45Pa8IF_evf18-aHbfHr_8fLNpotS26XTg7A2YJSG6XYDGpzaCgQtmLUanXZWah6Fc5ZvGUdnmeU9s1FLLnsQ4oK8Punu121zEXFeSkh-X8YplIPPYfR_d-Zx56_yjRcgheW8Cby4FSj5-4p18dNYI6YUZsxr9VZLY5xy0Mjn_5DXeS1zc3eEwBptWYPgBLU91lpwuPsKMH-MzZ9i8y02f4zNqzbz7E8PdxO_cmoAPwG1teYrLL9f_r_qTymLoso</recordid><startdate>20110601</startdate><enddate>20110601</enddate><creator>Vickers, Dwayne A. 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| ispartof | Biomedical microdevices, 2011-06, Vol.13 (3), p.565-571 |
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| subjects | B-Lymphocytes - cytology B-Lymphocytes - metabolism Biological and Medical Physics Biomedical engineering Biomedical Engineering and Bioengineering Biophysics Blood Cell Adhesion Cell adhesion & migration Cell Separation - instrumentation Engineering Engineering Fluid Dynamics Fluid dynamics Humans Immobilized Proteins - chemistry Immobilized Proteins - metabolism Jurkat Cells Leukemia Leukemia - blood Lymphocytes Microelectromechanical systems Microfluidic Analytical Techniques - instrumentation Nanotechnology Plant Lectins - chemistry Plant Lectins - metabolism T-Lymphocytes - cytology T-Lymphocytes - metabolism |
| title | Lectin-mediated microfluidic capture and release of leukemic lymphocytes from whole blood |
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