A Comprehensive GC–MS Sub-Microscale Assay for Fatty Acids and its Applications
Fatty acid analysis is essential to a broad range of applications including those associated with the nascent algal biofuel and algal bioproduct industries. Current fatty acid profiling methods require lengthy, sequential extraction and transesterification steps necessitating significant quantities...
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| Veröffentlicht in: | Journal of the American Oil Chemists' Society 2011-09, Vol.88 (9), p.1329-1338 |
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| container_title | Journal of the American Oil Chemists' Society |
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| creator | Bigelow, Nicholas W. Hardin, William R. Barker, James P. Ryken, Scott A. MacRae, Alex C. Cattolico, Rose Ann |
| description | Fatty acid analysis is essential to a broad range of applications including those associated with the nascent algal biofuel and algal bioproduct industries. Current fatty acid profiling methods require lengthy, sequential extraction and transesterification steps necessitating significant quantities of analyte. We report the development of a rapid, microscale, single-step, in situ protocol for GC–MS lipid analysis that requires only 250 μg dry mass per sample. We furthermore demonstrate the broad applications of this technique by profiling the fatty acids of several algal species, small aquatic organisms, insects and terrestrial plant material. When combined with fluorescent techniques utilizing the BODIPY dye family and flow cytometry, this micro-assay serves as a powerful tool for analyzing fatty acids in laboratory and field collected samples, for high-throughput screening, and for crop assessment. Additionally, the high sensitivity of the technique allows for population analyses across a wide variety of taxa. |
| doi_str_mv | 10.1007/s11746-011-1799-7 |
| format | Article |
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Current fatty acid profiling methods require lengthy, sequential extraction and transesterification steps necessitating significant quantities of analyte. We report the development of a rapid, microscale, single-step, in situ protocol for GC–MS lipid analysis that requires only 250 μg dry mass per sample. We furthermore demonstrate the broad applications of this technique by profiling the fatty acids of several algal species, small aquatic organisms, insects and terrestrial plant material. When combined with fluorescent techniques utilizing the BODIPY dye family and flow cytometry, this micro-assay serves as a powerful tool for analyzing fatty acids in laboratory and field collected samples, for high-throughput screening, and for crop assessment. 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Current fatty acid profiling methods require lengthy, sequential extraction and transesterification steps necessitating significant quantities of analyte. We report the development of a rapid, microscale, single-step, in situ protocol for GC–MS lipid analysis that requires only 250 μg dry mass per sample. We furthermore demonstrate the broad applications of this technique by profiling the fatty acids of several algal species, small aquatic organisms, insects and terrestrial plant material. When combined with fluorescent techniques utilizing the BODIPY dye family and flow cytometry, this micro-assay serves as a powerful tool for analyzing fatty acids in laboratory and field collected samples, for high-throughput screening, and for crop assessment. 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Current fatty acid profiling methods require lengthy, sequential extraction and transesterification steps necessitating significant quantities of analyte. We report the development of a rapid, microscale, single-step, in situ protocol for GC–MS lipid analysis that requires only 250 μg dry mass per sample. We furthermore demonstrate the broad applications of this technique by profiling the fatty acids of several algal species, small aquatic organisms, insects and terrestrial plant material. When combined with fluorescent techniques utilizing the BODIPY dye family and flow cytometry, this micro-assay serves as a powerful tool for analyzing fatty acids in laboratory and field collected samples, for high-throughput screening, and for crop assessment. 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| ispartof | Journal of the American Oil Chemists' Society, 2011-09, Vol.88 (9), p.1329-1338 |
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| source | SpringerNature Journals; Access via Wiley Online Library |
| subjects | Agriculture Algae Aquatic insects Aquatic organisms Biobased products Biodiesel fuels Biofuel production Biofuels Biofuels (Energy) Biological and medical sciences Biomaterials Biotechnology Biotechnology biocatalysis Breeding Chemistry Chemistry and Materials Science Chromatography Energy Fat industries Fats and oils Fatty acids Food and feed science Food industries Food Science Functional foods Fundamental and applied biological sciences. Psychology Genetics Genomics Industrial applications and implications. Economical aspects Industrial Chemistry/Chemical Engineering Lipid analysis Lipid chemistry Nutraceuticals Nutrition and health Oils & fats Original Paper |
| title | A Comprehensive GC–MS Sub-Microscale Assay for Fatty Acids and its Applications |
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