Maternal/fetal mortality and fetal growth restriction: role of nitric oxide and virulence factors in intrauterine infection in rats
Objective The mechanism of infection-related deaths of pregnant rats and intrauterine growth restriction are not understood. We assessed whether nitric oxide (NO) has differential effects on infection with Escherichia coli Dr/Afa mutants that lack either AfaE or AfaD invasins. Study Design Sprague-D...
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| Veröffentlicht in: | American journal of obstetrics and gynecology 2011-07, Vol.205 (1), p.83.e1-83.e7 |
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| container_title | American journal of obstetrics and gynecology |
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| creator | Wroblewska-Seniuk, Katarzyna, MD Nowicki, Stella, DDS Le Bouguénec, Chantal, PhD Nowicki, Bogdan, PhD Yallampalli, Chandra, PhD |
| description | Objective The mechanism of infection-related deaths of pregnant rats and intrauterine growth restriction are not understood. We assessed whether nitric oxide (NO) has differential effects on infection with Escherichia coli Dr/Afa mutants that lack either AfaE or AfaD invasins. Study Design Sprague-Dawley rats were infected intrauterinally with the clinical strain of E coli AfaE+ D+ or 1 of its isogenic mutants in the presence or absence of the NO synthesis inhibitor NG -nitro-L-arginine methyl ester (L-NAME). Maternal/fetal mortality rates, fetoplacental weight, and infection rates were evaluated. Results Maternal and/or fetal death was associated with the presence of at least 1 virulence factor (AfaE+ D+ >AfaE+ D− >AfaE− D+ ) and was increased by L-NAME treatment. The fetal and placental weights were lower than controls and were further reduced by L-NAME treatment. Conclusion These results demonstrate that NO enhanced AfaE- and AfaD-mediated virulence and plays an important role in Dr/Afa+ E coli gestational tropism. |
| doi_str_mv | 10.1016/j.ajog.2011.02.049 |
| format | Article |
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We assessed whether nitric oxide (NO) has differential effects on infection with Escherichia coli Dr/Afa mutants that lack either AfaE or AfaD invasins. Study Design Sprague-Dawley rats were infected intrauterinally with the clinical strain of E coli AfaE+ D+ or 1 of its isogenic mutants in the presence or absence of the NO synthesis inhibitor NG -nitro-L-arginine methyl ester (L-NAME). Maternal/fetal mortality rates, fetoplacental weight, and infection rates were evaluated. Results Maternal and/or fetal death was associated with the presence of at least 1 virulence factor (AfaE+ D+ >AfaE+ D− >AfaE− D+ ) and was increased by L-NAME treatment. The fetal and placental weights were lower than controls and were further reduced by L-NAME treatment. Conclusion These results demonstrate that NO enhanced AfaE- and AfaD-mediated virulence and plays an important role in Dr/Afa+ E coli gestational tropism.</description><identifier>ISSN: 0002-9378</identifier><identifier>ISSN: 1097-6868</identifier><identifier>EISSN: 1097-6868</identifier><identifier>DOI: 10.1016/j.ajog.2011.02.049</identifier><identifier>PMID: 21481839</identifier><language>eng</language><publisher>United States: Mosby, Inc</publisher><subject>adhesive structure ; Animals ; Dr family ; Enzyme Inhibitors - pharmacology ; Escherichia coli ; Escherichia coli - drug effects ; Escherichia coli Infections - chemically induced ; Escherichia coli Infections - mortality ; Female ; Fetal Growth Retardation - chemically induced ; Fetal Growth Retardation - microbiology ; Fetal Growth Retardation - mortality ; Fetal Mortality ; Fetus - drug effects ; growth restriction ; intrauterine infection ; L-NAME ; Maternal Mortality ; mortality rate ; NG-Nitroarginine Methyl Ester - pharmacology ; nitric oxide ; Nitric Oxide - antagonists & inhibitors ; Nitric Oxide - biosynthesis ; Nitric Oxide Synthase - antagonists & inhibitors ; Obstetrics and Gynecology ; Pregnancy ; Pregnancy Complications, Infectious - chemically induced ; rat ; Rats ; Rats, Sprague-Dawley ; Uterine Diseases - chemically induced ; Uterine Diseases - microbiology ; Uterine Diseases - mortality ; Virulence Factors - metabolism</subject><ispartof>American journal of obstetrics and gynecology, 2011-07, Vol.205 (1), p.83.e1-83.e7</ispartof><rights>Mosby, Inc.</rights><rights>2011 Mosby, Inc.</rights><rights>Copyright © 2011 Mosby, Inc. All rights reserved.</rights><rights>2011 Mosby, Inc. All rights reserved. 2011</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c509t-671b1cafd1bf8f1da9795c2e5f2b16f067f23ef88566832b50f893d614bcb4f43</citedby><cites>FETCH-LOGICAL-c509t-671b1cafd1bf8f1da9795c2e5f2b16f067f23ef88566832b50f893d614bcb4f43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0002937811002390$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,776,780,881,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21481839$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wroblewska-Seniuk, Katarzyna, MD</creatorcontrib><creatorcontrib>Nowicki, Stella, DDS</creatorcontrib><creatorcontrib>Le Bouguénec, Chantal, PhD</creatorcontrib><creatorcontrib>Nowicki, Bogdan, PhD</creatorcontrib><creatorcontrib>Yallampalli, Chandra, PhD</creatorcontrib><title>Maternal/fetal mortality and fetal growth restriction: role of nitric oxide and virulence factors in intrauterine infection in rats</title><title>American journal of obstetrics and gynecology</title><addtitle>Am J Obstet Gynecol</addtitle><description>Objective The mechanism of infection-related deaths of pregnant rats and intrauterine growth restriction are not understood. We assessed whether nitric oxide (NO) has differential effects on infection with Escherichia coli Dr/Afa mutants that lack either AfaE or AfaD invasins. Study Design Sprague-Dawley rats were infected intrauterinally with the clinical strain of E coli AfaE+ D+ or 1 of its isogenic mutants in the presence or absence of the NO synthesis inhibitor NG -nitro-L-arginine methyl ester (L-NAME). Maternal/fetal mortality rates, fetoplacental weight, and infection rates were evaluated. Results Maternal and/or fetal death was associated with the presence of at least 1 virulence factor (AfaE+ D+ >AfaE+ D− >AfaE− D+ ) and was increased by L-NAME treatment. The fetal and placental weights were lower than controls and were further reduced by L-NAME treatment. Conclusion These results demonstrate that NO enhanced AfaE- and AfaD-mediated virulence and plays an important role in Dr/Afa+ E coli gestational tropism.</description><subject>adhesive structure</subject><subject>Animals</subject><subject>Dr family</subject><subject>Enzyme Inhibitors - pharmacology</subject><subject>Escherichia coli</subject><subject>Escherichia coli - drug effects</subject><subject>Escherichia coli Infections - chemically induced</subject><subject>Escherichia coli Infections - mortality</subject><subject>Female</subject><subject>Fetal Growth Retardation - chemically induced</subject><subject>Fetal Growth Retardation - microbiology</subject><subject>Fetal Growth Retardation - mortality</subject><subject>Fetal Mortality</subject><subject>Fetus - drug effects</subject><subject>growth restriction</subject><subject>intrauterine infection</subject><subject>L-NAME</subject><subject>Maternal Mortality</subject><subject>mortality rate</subject><subject>NG-Nitroarginine Methyl Ester - pharmacology</subject><subject>nitric oxide</subject><subject>Nitric Oxide - antagonists & inhibitors</subject><subject>Nitric Oxide - biosynthesis</subject><subject>Nitric Oxide Synthase - antagonists & inhibitors</subject><subject>Obstetrics and Gynecology</subject><subject>Pregnancy</subject><subject>Pregnancy Complications, Infectious - chemically induced</subject><subject>rat</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Uterine Diseases - chemically induced</subject><subject>Uterine Diseases - microbiology</subject><subject>Uterine Diseases - mortality</subject><subject>Virulence Factors - metabolism</subject><issn>0002-9378</issn><issn>1097-6868</issn><issn>1097-6868</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9Uktv1DAQjhCILoU_wAH5ximpH4ljI1QJVbykIg7A2XKc8dbBGxc7Wdgzfxy7WyrggGRpPI_vm9F8U1VPCW4IJvxsavQUtg3FhDSYNriV96oNwbKvueDifrXBGNNasl6cVI9SmopLJX1YnVDSCiKY3FQ_P-gF4qz9mYVFe7QLMRu3HJCeR3SMbWP4vlyhCGmJziwuzC9QDB5QsGh2JYbCDzfCDWTv4uphNoCsNkuICbk5vyXqNTdyM2THwg1LyUS9pMfVA6t9gie39rT68ub154t39eXHt-8vXl3WpsNyqXlPBmK0HclghSWjlr3sDIXO0oFwi3lvKQMrRMe5YHTosBWSjZy0gxla27LT6vzIe70OOxgNlKm8uo5up-NBBe3U35nZXalt2CtGWkZbngme3xLE8G3N61A7lwx4r2cIa1ISd7xnrMW5kh4rTQwpRbB3XQhWRTw1qSKeKuIpTFUWL4Oe_TnfHeS3Wrng5bEA8pb2DqJKxpVdjy7mlaoxuP_zn_8DN97Nzmj_FQ6QprCWQ0iKqJQB6lO5l3I9hOQPk5j9Ao2BxGU</recordid><startdate>20110701</startdate><enddate>20110701</enddate><creator>Wroblewska-Seniuk, Katarzyna, MD</creator><creator>Nowicki, Stella, DDS</creator><creator>Le Bouguénec, Chantal, PhD</creator><creator>Nowicki, Bogdan, PhD</creator><creator>Yallampalli, Chandra, PhD</creator><general>Mosby, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20110701</creationdate><title>Maternal/fetal mortality and fetal growth restriction: role of nitric oxide and virulence factors in intrauterine infection in rats</title><author>Wroblewska-Seniuk, Katarzyna, MD ; Nowicki, Stella, DDS ; Le Bouguénec, Chantal, PhD ; Nowicki, Bogdan, PhD ; Yallampalli, Chandra, PhD</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c509t-671b1cafd1bf8f1da9795c2e5f2b16f067f23ef88566832b50f893d614bcb4f43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>adhesive structure</topic><topic>Animals</topic><topic>Dr family</topic><topic>Enzyme Inhibitors - pharmacology</topic><topic>Escherichia coli</topic><topic>Escherichia coli - drug effects</topic><topic>Escherichia coli Infections - chemically induced</topic><topic>Escherichia coli Infections - mortality</topic><topic>Female</topic><topic>Fetal Growth Retardation - chemically induced</topic><topic>Fetal Growth Retardation - microbiology</topic><topic>Fetal Growth Retardation - mortality</topic><topic>Fetal Mortality</topic><topic>Fetus - drug effects</topic><topic>growth restriction</topic><topic>intrauterine infection</topic><topic>L-NAME</topic><topic>Maternal Mortality</topic><topic>mortality rate</topic><topic>NG-Nitroarginine Methyl Ester - pharmacology</topic><topic>nitric oxide</topic><topic>Nitric Oxide - antagonists & inhibitors</topic><topic>Nitric Oxide - biosynthesis</topic><topic>Nitric Oxide Synthase - antagonists & inhibitors</topic><topic>Obstetrics and Gynecology</topic><topic>Pregnancy</topic><topic>Pregnancy Complications, Infectious - chemically induced</topic><topic>rat</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Uterine Diseases - chemically induced</topic><topic>Uterine Diseases - microbiology</topic><topic>Uterine Diseases - mortality</topic><topic>Virulence Factors - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wroblewska-Seniuk, Katarzyna, MD</creatorcontrib><creatorcontrib>Nowicki, Stella, DDS</creatorcontrib><creatorcontrib>Le Bouguénec, Chantal, PhD</creatorcontrib><creatorcontrib>Nowicki, Bogdan, PhD</creatorcontrib><creatorcontrib>Yallampalli, Chandra, PhD</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>American journal of obstetrics and gynecology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wroblewska-Seniuk, Katarzyna, MD</au><au>Nowicki, Stella, DDS</au><au>Le Bouguénec, Chantal, PhD</au><au>Nowicki, Bogdan, PhD</au><au>Yallampalli, Chandra, PhD</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Maternal/fetal mortality and fetal growth restriction: role of nitric oxide and virulence factors in intrauterine infection in rats</atitle><jtitle>American journal of obstetrics and gynecology</jtitle><addtitle>Am J Obstet Gynecol</addtitle><date>2011-07-01</date><risdate>2011</risdate><volume>205</volume><issue>1</issue><spage>83.e1</spage><epage>83.e7</epage><pages>83.e1-83.e7</pages><issn>0002-9378</issn><issn>1097-6868</issn><eissn>1097-6868</eissn><abstract>Objective The mechanism of infection-related deaths of pregnant rats and intrauterine growth restriction are not understood. We assessed whether nitric oxide (NO) has differential effects on infection with Escherichia coli Dr/Afa mutants that lack either AfaE or AfaD invasins. Study Design Sprague-Dawley rats were infected intrauterinally with the clinical strain of E coli AfaE+ D+ or 1 of its isogenic mutants in the presence or absence of the NO synthesis inhibitor NG -nitro-L-arginine methyl ester (L-NAME). Maternal/fetal mortality rates, fetoplacental weight, and infection rates were evaluated. Results Maternal and/or fetal death was associated with the presence of at least 1 virulence factor (AfaE+ D+ >AfaE+ D− >AfaE− D+ ) and was increased by L-NAME treatment. The fetal and placental weights were lower than controls and were further reduced by L-NAME treatment. Conclusion These results demonstrate that NO enhanced AfaE- and AfaD-mediated virulence and plays an important role in Dr/Afa+ E coli gestational tropism.</abstract><cop>United States</cop><pub>Mosby, Inc</pub><pmid>21481839</pmid><doi>10.1016/j.ajog.2011.02.049</doi><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | adhesive structure Animals Dr family Enzyme Inhibitors - pharmacology Escherichia coli Escherichia coli - drug effects Escherichia coli Infections - chemically induced Escherichia coli Infections - mortality Female Fetal Growth Retardation - chemically induced Fetal Growth Retardation - microbiology Fetal Growth Retardation - mortality Fetal Mortality Fetus - drug effects growth restriction intrauterine infection L-NAME Maternal Mortality mortality rate NG-Nitroarginine Methyl Ester - pharmacology nitric oxide Nitric Oxide - antagonists & inhibitors Nitric Oxide - biosynthesis Nitric Oxide Synthase - antagonists & inhibitors Obstetrics and Gynecology Pregnancy Pregnancy Complications, Infectious - chemically induced rat Rats Rats, Sprague-Dawley Uterine Diseases - chemically induced Uterine Diseases - microbiology Uterine Diseases - mortality Virulence Factors - metabolism |
| title | Maternal/fetal mortality and fetal growth restriction: role of nitric oxide and virulence factors in intrauterine infection in rats |
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