Characterization of the oxysterol-binding protein gene family in the yellow fever mosquito, Aedes aegypti
The oxysterol-binding protein (OSBP) and OSBP-related proteins (ORPs) are sterol-binding proteins that may be involved in cellular sterol transportation, sterol metabolism and signal transduction pathways. Four ORP genes were cloned from Aedes aegypti. Based on amino acid sequence homology to human...
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| Veröffentlicht in: | Insect molecular biology 2011-08, Vol.20 (4), p.541-552 |
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| creator | Fu, Q Lynn-Miller, A Lan, Q |
| description | The oxysterol-binding protein (OSBP) and OSBP-related proteins (ORPs) are sterol-binding proteins that may be involved in cellular sterol transportation, sterol metabolism and signal transduction pathways. Four ORP genes were cloned from Aedes aegypti. Based on amino acid sequence homology to human proteins, they are AeOSBP, AeORP1, AeORP8 and AeORP9. Splicing variants of AeOSBP and AeORP8 were identified. The temporal and spatial transcription patterns of members of the AeOSBP gene family through developmental stages and the gonotrophic cycle were profiled. AeORP1 transcription seemed to be head tissue-specific, whereas AeOSBP and AeORP9 expression was induced by a bloodmeal. Furthermore, over-expression of AeORPs facilitated [³H]-cholesterol uptake in Ae. aegypti cultured Aag -2 cells. |
| doi_str_mv | 10.1111/j.1365-2583.2011.01087.x |
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Four ORP genes were cloned from Aedes aegypti. Based on amino acid sequence homology to human proteins, they are AeOSBP, AeORP1, AeORP8 and AeORP9. Splicing variants of AeOSBP and AeORP8 were identified. The temporal and spatial transcription patterns of members of the AeOSBP gene family through developmental stages and the gonotrophic cycle were profiled. AeORP1 transcription seemed to be head tissue-specific, whereas AeOSBP and AeORP9 expression was induced by a bloodmeal. Furthermore, over-expression of AeORPs facilitated [³H]-cholesterol uptake in Ae. aegypti cultured Aag -2 cells.</description><identifier>ISSN: 0962-1075</identifier><identifier>EISSN: 1365-2583</identifier><identifier>DOI: 10.1111/j.1365-2583.2011.01087.x</identifier><identifier>PMID: 21699592</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Aedes - genetics ; Aedes - metabolism ; Aedes aegypti ; amino acid sequences ; Animals ; Cell Line ; cholesterol ; Cholesterol - metabolism ; developmental stages ; Female ; gene expression ; genes ; gonotrophic cycle ; head ; humans ; Insect Vectors - genetics ; Insect Vectors - metabolism ; Male ; metabolism ; Multigene Family ; oxysterol-binding protein ; proteins ; Receptors, Steroid - genetics ; Receptors, Steroid - metabolism ; sequence homology ; signal transduction ; sterol transport ; Transcription, Genetic ; Up-Regulation ; Yellow Fever - transmission</subject><ispartof>Insect molecular biology, 2011-08, Vol.20 (4), p.541-552</ispartof><rights>2011 The Authors. Insect Molecular Biology © 2011 The Royal Entomological Society</rights><rights>2011 The Authors. Insect Molecular Biology © 2011 The Royal Entomological Society.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5697-952db3d89ff185a745d0bf11091ba4f4309151d1031db95dbefc99e0d216c0193</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1365-2583.2011.01087.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1365-2583.2011.01087.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>230,314,776,780,881,1411,27903,27904,45553,45554</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21699592$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fu, Q</creatorcontrib><creatorcontrib>Lynn-Miller, A</creatorcontrib><creatorcontrib>Lan, Q</creatorcontrib><title>Characterization of the oxysterol-binding protein gene family in the yellow fever mosquito, Aedes aegypti</title><title>Insect molecular biology</title><addtitle>Insect Mol Biol</addtitle><description>The oxysterol-binding protein (OSBP) and OSBP-related proteins (ORPs) are sterol-binding proteins that may be involved in cellular sterol transportation, sterol metabolism and signal transduction pathways. Four ORP genes were cloned from Aedes aegypti. Based on amino acid sequence homology to human proteins, they are AeOSBP, AeORP1, AeORP8 and AeORP9. Splicing variants of AeOSBP and AeORP8 were identified. The temporal and spatial transcription patterns of members of the AeOSBP gene family through developmental stages and the gonotrophic cycle were profiled. AeORP1 transcription seemed to be head tissue-specific, whereas AeOSBP and AeORP9 expression was induced by a bloodmeal. Furthermore, over-expression of AeORPs facilitated [³H]-cholesterol uptake in Ae. aegypti cultured Aag -2 cells.</description><subject>Aedes - genetics</subject><subject>Aedes - metabolism</subject><subject>Aedes aegypti</subject><subject>amino acid sequences</subject><subject>Animals</subject><subject>Cell Line</subject><subject>cholesterol</subject><subject>Cholesterol - metabolism</subject><subject>developmental stages</subject><subject>Female</subject><subject>gene expression</subject><subject>genes</subject><subject>gonotrophic cycle</subject><subject>head</subject><subject>humans</subject><subject>Insect Vectors - genetics</subject><subject>Insect Vectors - metabolism</subject><subject>Male</subject><subject>metabolism</subject><subject>Multigene Family</subject><subject>oxysterol-binding protein</subject><subject>proteins</subject><subject>Receptors, Steroid - genetics</subject><subject>Receptors, Steroid - metabolism</subject><subject>sequence homology</subject><subject>signal transduction</subject><subject>sterol transport</subject><subject>Transcription, Genetic</subject><subject>Up-Regulation</subject><subject>Yellow Fever - transmission</subject><issn>0962-1075</issn><issn>1365-2583</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkk1v1DAQhiMEotvCXwCLCxcSPHEcxweQyhZKUSmoUHocOYmz620Sb52k3fDrcdiyAg7gi8eeZ17NVxAQoBH483IVAUt5GPOMRTEFiCjQTESbe8Fs57gfzKhM4xCo4HvBftetKKWZTOXDYC-GVEou41lg5kvlVNFrZ76r3tiW2Ir0S03sZuz8r63D3LSlaRdk7WyvTUsWutWkUo2pR-KfEzzqura3pNI32pHGdteD6e0LcqhL3RGlF-O6N4-CB5WqO_347j4ILt69_Tp_H55-Oj6ZH56GBU-lCCWPy5yVmawqyLgSCS9pXgFQCblKqoR5g0MJlEGZS17muiqk1LT0NRUUJDsIXm9110Pe6LLQbe9UjWtnGuVGtMrgn57WLHFhb5ABk75DXuD5nYCz14PuemxMV_gKVavt0GGWZb6xINP_kyKjjApgnnz2F7myg2t9HzwkkoylIvHQk98z36X8a1oeeLUFbk2tx50fKE5bgSucho_T8HHaCvy5FbjBk49vJsvHh9t440e72cUrd4WpYILj5dkxnn8-h2-XZ0f4wfNPt3ylLKqFMx1efPHKid8klnBO_0mAoL7yH6cHzY8</recordid><startdate>201108</startdate><enddate>201108</enddate><creator>Fu, Q</creator><creator>Lynn-Miller, A</creator><creator>Lan, Q</creator><general>Blackwell Publishing Ltd</general><scope>FBQ</scope><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7QR</scope><scope>7SS</scope><scope>7TK</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>7U9</scope><scope>C1K</scope><scope>F1W</scope><scope>H94</scope><scope>H95</scope><scope>H97</scope><scope>L.G</scope><scope>5PM</scope></search><sort><creationdate>201108</creationdate><title>Characterization of the oxysterol-binding protein gene family in the yellow fever mosquito, Aedes aegypti</title><author>Fu, Q ; Lynn-Miller, A ; Lan, Q</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5697-952db3d89ff185a745d0bf11091ba4f4309151d1031db95dbefc99e0d216c0193</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Aedes - genetics</topic><topic>Aedes - metabolism</topic><topic>Aedes aegypti</topic><topic>amino acid sequences</topic><topic>Animals</topic><topic>Cell Line</topic><topic>cholesterol</topic><topic>Cholesterol - metabolism</topic><topic>developmental stages</topic><topic>Female</topic><topic>gene expression</topic><topic>genes</topic><topic>gonotrophic cycle</topic><topic>head</topic><topic>humans</topic><topic>Insect Vectors - genetics</topic><topic>Insect Vectors - metabolism</topic><topic>Male</topic><topic>metabolism</topic><topic>Multigene Family</topic><topic>oxysterol-binding protein</topic><topic>proteins</topic><topic>Receptors, Steroid - genetics</topic><topic>Receptors, Steroid - metabolism</topic><topic>sequence homology</topic><topic>signal transduction</topic><topic>sterol transport</topic><topic>Transcription, Genetic</topic><topic>Up-Regulation</topic><topic>Yellow Fever - transmission</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fu, Q</creatorcontrib><creatorcontrib>Lynn-Miller, A</creatorcontrib><creatorcontrib>Lan, Q</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Chemoreception Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Virology and AIDS Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Insect molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fu, Q</au><au>Lynn-Miller, A</au><au>Lan, Q</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of the oxysterol-binding protein gene family in the yellow fever mosquito, Aedes aegypti</atitle><jtitle>Insect molecular biology</jtitle><addtitle>Insect Mol Biol</addtitle><date>2011-08</date><risdate>2011</risdate><volume>20</volume><issue>4</issue><spage>541</spage><epage>552</epage><pages>541-552</pages><issn>0962-1075</issn><eissn>1365-2583</eissn><abstract>The oxysterol-binding protein (OSBP) and OSBP-related proteins (ORPs) are sterol-binding proteins that may be involved in cellular sterol transportation, sterol metabolism and signal transduction pathways. Four ORP genes were cloned from Aedes aegypti. Based on amino acid sequence homology to human proteins, they are AeOSBP, AeORP1, AeORP8 and AeORP9. Splicing variants of AeOSBP and AeORP8 were identified. The temporal and spatial transcription patterns of members of the AeOSBP gene family through developmental stages and the gonotrophic cycle were profiled. AeORP1 transcription seemed to be head tissue-specific, whereas AeOSBP and AeORP9 expression was induced by a bloodmeal. Furthermore, over-expression of AeORPs facilitated [³H]-cholesterol uptake in Ae. aegypti cultured Aag -2 cells.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>21699592</pmid><doi>10.1111/j.1365-2583.2011.01087.x</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Insect molecular biology, 2011-08, Vol.20 (4), p.541-552 |
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| subjects | Aedes - genetics Aedes - metabolism Aedes aegypti amino acid sequences Animals Cell Line cholesterol Cholesterol - metabolism developmental stages Female gene expression genes gonotrophic cycle head humans Insect Vectors - genetics Insect Vectors - metabolism Male metabolism Multigene Family oxysterol-binding protein proteins Receptors, Steroid - genetics Receptors, Steroid - metabolism sequence homology signal transduction sterol transport Transcription, Genetic Up-Regulation Yellow Fever - transmission |
| title | Characterization of the oxysterol-binding protein gene family in the yellow fever mosquito, Aedes aegypti |
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