Endogenous siRNAs Derived from a Pair of Natural cis-Antisense Transcripts Regulate Salt Tolerance in Arabidopsis
In higher eukaryotes, miRNAs and siRNAs guide translational inhibition, mRNA cleavage, or chromatin regulation. We found that the antisense overlapping gene pair of Δ 1-pyrroline-5-carboxylate dehydrogenase ( P5CDH), a stress-related gene, and SRO5, a gene of unknown function, generates two types of...
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| Veröffentlicht in: | Cell 2005-12, Vol.123 (7), p.1279-1291 |
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| creator | Borsani, Omar Zhu, Jianhua Verslues, Paul E. Sunkar, Ramanjulu Zhu, Jian-Kang |
| description | In higher eukaryotes, miRNAs and siRNAs guide translational inhibition, mRNA cleavage, or chromatin regulation. We found that the antisense overlapping gene pair of Δ
1-pyrroline-5-carboxylate dehydrogenase (
P5CDH), a stress-related gene, and
SRO5, a gene of unknown function, generates two types of siRNAs. When both transcripts are present, a 24-nt siRNA is formed by a biogenesis pathway dependent on DCL2, RDR6, SGS3, and NRPD1A. Initial cleavage of the
P5CDH transcript guided by the 24-nt siRNA establishes a phase for the subsequent generation of 21-nt siRNAs by DCL1 and further cleavage of
P5CDH transcripts. The expression of
SRO5 is induced by salt, and this induction is required to initiate siRNA formation. Our data suggest that the P5CDH and SRO5 proteins are also functionally related, and that the
P5CDH-SRO5 gene pair defines a mode of siRNA function and biogenesis that may be applied to other natural
cis-antisense gene pairs in eukaryotic genomes. |
| doi_str_mv | 10.1016/j.cell.2005.11.035 |
| format | Article |
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1-pyrroline-5-carboxylate dehydrogenase (
P5CDH), a stress-related gene, and
SRO5, a gene of unknown function, generates two types of siRNAs. When both transcripts are present, a 24-nt siRNA is formed by a biogenesis pathway dependent on DCL2, RDR6, SGS3, and NRPD1A. Initial cleavage of the
P5CDH transcript guided by the 24-nt siRNA establishes a phase for the subsequent generation of 21-nt siRNAs by DCL1 and further cleavage of
P5CDH transcripts. The expression of
SRO5 is induced by salt, and this induction is required to initiate siRNA formation. Our data suggest that the P5CDH and SRO5 proteins are also functionally related, and that the
P5CDH-SRO5 gene pair defines a mode of siRNA function and biogenesis that may be applied to other natural
cis-antisense gene pairs in eukaryotic genomes.</description><identifier>ISSN: 0092-8674</identifier><identifier>EISSN: 1097-4172</identifier><identifier>DOI: 10.1016/j.cell.2005.11.035</identifier><identifier>PMID: 16377568</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>1-Pyrroline-5-Carboxylate Dehydrogenase - genetics ; 1-Pyrroline-5-Carboxylate Dehydrogenase - metabolism ; Arabidopsis ; Arabidopsis - drug effects ; Arabidopsis - genetics ; Arabidopsis - physiology ; Arabidopsis Proteins - genetics ; Arabidopsis Proteins - metabolism ; Base Sequence ; Down-Regulation - drug effects ; Down-Regulation - physiology ; Models, Biological ; Molecular Sequence Data ; Mutation ; Proline - metabolism ; RNA, Antisense - genetics ; RNA, Small Interfering - biosynthesis ; RNA, Small Interfering - physiology ; Salts - metabolism ; Salts - pharmacology ; Up-Regulation - drug effects ; Up-Regulation - physiology</subject><ispartof>Cell, 2005-12, Vol.123 (7), p.1279-1291</ispartof><rights>2005 Elsevier Inc.</rights><rights>2005 Elsevier Inc. 2005</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c484t-b84fe7acf65b4adfdfbb825c953b4bdd185c699ffea48e969af5abd8c61d12da3</citedby><cites>FETCH-LOGICAL-c484t-b84fe7acf65b4adfdfbb825c953b4bdd185c699ffea48e969af5abd8c61d12da3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0092867405013309$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>230,314,776,780,881,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16377568$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Borsani, Omar</creatorcontrib><creatorcontrib>Zhu, Jianhua</creatorcontrib><creatorcontrib>Verslues, Paul E.</creatorcontrib><creatorcontrib>Sunkar, Ramanjulu</creatorcontrib><creatorcontrib>Zhu, Jian-Kang</creatorcontrib><title>Endogenous siRNAs Derived from a Pair of Natural cis-Antisense Transcripts Regulate Salt Tolerance in Arabidopsis</title><title>Cell</title><addtitle>Cell</addtitle><description>In higher eukaryotes, miRNAs and siRNAs guide translational inhibition, mRNA cleavage, or chromatin regulation. We found that the antisense overlapping gene pair of Δ
1-pyrroline-5-carboxylate dehydrogenase (
P5CDH), a stress-related gene, and
SRO5, a gene of unknown function, generates two types of siRNAs. When both transcripts are present, a 24-nt siRNA is formed by a biogenesis pathway dependent on DCL2, RDR6, SGS3, and NRPD1A. Initial cleavage of the
P5CDH transcript guided by the 24-nt siRNA establishes a phase for the subsequent generation of 21-nt siRNAs by DCL1 and further cleavage of
P5CDH transcripts. The expression of
SRO5 is induced by salt, and this induction is required to initiate siRNA formation. Our data suggest that the P5CDH and SRO5 proteins are also functionally related, and that the
P5CDH-SRO5 gene pair defines a mode of siRNA function and biogenesis that may be applied to other natural
cis-antisense gene pairs in eukaryotic genomes.</description><subject>1-Pyrroline-5-Carboxylate Dehydrogenase - genetics</subject><subject>1-Pyrroline-5-Carboxylate Dehydrogenase - metabolism</subject><subject>Arabidopsis</subject><subject>Arabidopsis - drug effects</subject><subject>Arabidopsis - genetics</subject><subject>Arabidopsis - physiology</subject><subject>Arabidopsis Proteins - genetics</subject><subject>Arabidopsis Proteins - metabolism</subject><subject>Base Sequence</subject><subject>Down-Regulation - drug effects</subject><subject>Down-Regulation - physiology</subject><subject>Models, Biological</subject><subject>Molecular Sequence Data</subject><subject>Mutation</subject><subject>Proline - metabolism</subject><subject>RNA, Antisense - genetics</subject><subject>RNA, Small Interfering - biosynthesis</subject><subject>RNA, Small Interfering - physiology</subject><subject>Salts - metabolism</subject><subject>Salts - pharmacology</subject><subject>Up-Regulation - drug effects</subject><subject>Up-Regulation - physiology</subject><issn>0092-8674</issn><issn>1097-4172</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUuP0zAUhS0EYjqFP8ACecUuwU5sJ5EQUjUPQBoNaChry4_r4iqNO7ZTiX-Pq1Y8NrC6i_udo3vPQegVJTUlVLzd1gbGsW4I4TWlNWn5E7SgZOgqRrvmKVoQMjRVLzp2gS5T2hJCes75c3RBRdt1XPQL9Hgz2bCBKcwJJ_9wv0r4GqI_gMUuhh1W-IvyEQeH71Weoxqx8alaTdknmBLgdVRTMtHvc8IPsJlHlQF_VWPG6zBCWRrAfsKrqLS3YZ98eoGeOTUmeHmeS_Tt9mZ99bG6-_zh09XqrjKsZ7nSPXPQKeME10xZZ53WfcPNwFvNtLW050YMg3OgWA-DGJTjStveCGppY1W7RO9PvvtZ78AamHI5X-6j36n4Qwbl5d-byX-Xm3CQLW07XhJaojdngxgeZ0hZ7nw6Jq4mKHFJMRDWtoT-F2xIwxgnvIDNCTQxpBTB_bqGEnmsVG7lUSePlUpKZam0iF7_-cdvybnDArw7AVDSPHiIMhkPJXjrI5gsbfD_8v8JpYy2Xw</recordid><startdate>20051229</startdate><enddate>20051229</enddate><creator>Borsani, Omar</creator><creator>Zhu, Jianhua</creator><creator>Verslues, Paul E.</creator><creator>Sunkar, Ramanjulu</creator><creator>Zhu, Jian-Kang</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20051229</creationdate><title>Endogenous siRNAs Derived from a Pair of Natural cis-Antisense Transcripts Regulate Salt Tolerance in Arabidopsis</title><author>Borsani, Omar ; Zhu, Jianhua ; Verslues, Paul E. ; Sunkar, Ramanjulu ; Zhu, Jian-Kang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c484t-b84fe7acf65b4adfdfbb825c953b4bdd185c699ffea48e969af5abd8c61d12da3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>1-Pyrroline-5-Carboxylate Dehydrogenase - genetics</topic><topic>1-Pyrroline-5-Carboxylate Dehydrogenase - metabolism</topic><topic>Arabidopsis</topic><topic>Arabidopsis - drug effects</topic><topic>Arabidopsis - genetics</topic><topic>Arabidopsis - physiology</topic><topic>Arabidopsis Proteins - genetics</topic><topic>Arabidopsis Proteins - metabolism</topic><topic>Base Sequence</topic><topic>Down-Regulation - drug effects</topic><topic>Down-Regulation - physiology</topic><topic>Models, Biological</topic><topic>Molecular Sequence Data</topic><topic>Mutation</topic><topic>Proline - metabolism</topic><topic>RNA, Antisense - genetics</topic><topic>RNA, Small Interfering - biosynthesis</topic><topic>RNA, Small Interfering - physiology</topic><topic>Salts - metabolism</topic><topic>Salts - pharmacology</topic><topic>Up-Regulation - drug effects</topic><topic>Up-Regulation - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Borsani, Omar</creatorcontrib><creatorcontrib>Zhu, Jianhua</creatorcontrib><creatorcontrib>Verslues, Paul E.</creatorcontrib><creatorcontrib>Sunkar, Ramanjulu</creatorcontrib><creatorcontrib>Zhu, Jian-Kang</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Cell</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Borsani, Omar</au><au>Zhu, Jianhua</au><au>Verslues, Paul E.</au><au>Sunkar, Ramanjulu</au><au>Zhu, Jian-Kang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Endogenous siRNAs Derived from a Pair of Natural cis-Antisense Transcripts Regulate Salt Tolerance in Arabidopsis</atitle><jtitle>Cell</jtitle><addtitle>Cell</addtitle><date>2005-12-29</date><risdate>2005</risdate><volume>123</volume><issue>7</issue><spage>1279</spage><epage>1291</epage><pages>1279-1291</pages><issn>0092-8674</issn><eissn>1097-4172</eissn><abstract>In higher eukaryotes, miRNAs and siRNAs guide translational inhibition, mRNA cleavage, or chromatin regulation. We found that the antisense overlapping gene pair of Δ
1-pyrroline-5-carboxylate dehydrogenase (
P5CDH), a stress-related gene, and
SRO5, a gene of unknown function, generates two types of siRNAs. When both transcripts are present, a 24-nt siRNA is formed by a biogenesis pathway dependent on DCL2, RDR6, SGS3, and NRPD1A. Initial cleavage of the
P5CDH transcript guided by the 24-nt siRNA establishes a phase for the subsequent generation of 21-nt siRNAs by DCL1 and further cleavage of
P5CDH transcripts. The expression of
SRO5 is induced by salt, and this induction is required to initiate siRNA formation. Our data suggest that the P5CDH and SRO5 proteins are also functionally related, and that the
P5CDH-SRO5 gene pair defines a mode of siRNA function and biogenesis that may be applied to other natural
cis-antisense gene pairs in eukaryotic genomes.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>16377568</pmid><doi>10.1016/j.cell.2005.11.035</doi><tpages>13</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Cell Press Free Archives; Elsevier ScienceDirect Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | 1-Pyrroline-5-Carboxylate Dehydrogenase - genetics 1-Pyrroline-5-Carboxylate Dehydrogenase - metabolism Arabidopsis Arabidopsis - drug effects Arabidopsis - genetics Arabidopsis - physiology Arabidopsis Proteins - genetics Arabidopsis Proteins - metabolism Base Sequence Down-Regulation - drug effects Down-Regulation - physiology Models, Biological Molecular Sequence Data Mutation Proline - metabolism RNA, Antisense - genetics RNA, Small Interfering - biosynthesis RNA, Small Interfering - physiology Salts - metabolism Salts - pharmacology Up-Regulation - drug effects Up-Regulation - physiology |
| title | Endogenous siRNAs Derived from a Pair of Natural cis-Antisense Transcripts Regulate Salt Tolerance in Arabidopsis |
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