Dioscorea Phytocompounds Enhance Murine Splenocyte Proliferation Ex Vivo and Improve Regeneration of Bone Marrow Cells In Vivo
Specific cytokines have been tested clinically for immunotherapy of cancers; however, cytotoxicity has often impaired their usefulness. Consequently, alternative approaches are increasingly desirable. Dioscorea spp. tuber is a widely used traditional Chinese medicinal herb claimed to confer immunost...
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| Veröffentlicht in: | Evidence-based complementary and alternative medicine 2011-01, Vol.2011 (2011), p.1-11 |
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| creator | Su, Pei-Fen Li, Chin-Jin Hsu, Chih-Chien Benson, Spencer Wang, Sheng-Yang Aravindaram, Kandan Chan, Sunney I. Wu, Shih-Hsiung Yang, Feng-Ling Huang, Wen-Ching Shyur, Lie-Fen Yang, Ning-Sun |
| description | Specific cytokines have been tested clinically for immunotherapy of cancers; however, cytotoxicity has often impaired their usefulness. Consequently, alternative approaches are increasingly desirable. Dioscorea spp. tuber is a widely used traditional Chinese medicinal herb claimed to confer immunostimulatory activity. In this study, we evaluated Dioscorea as an adjuvant therapy for use alongside chemotherapy for cancer. Phytocompounds from Dioscorea tubers were ethanol fractioned and used for ex vivo splenocyte proliferation assay or in vivo force-feeding of mice pre-treated with the chemotherapy agent 5-fluorouracil. Co-treatment with a 50–75% ethanol-partitioned fraction of the tuber extract of D. batatas (DsCE-II) and interleukin (IL)-2 resulted in a significantly higher rate of murine splenocyte cell proliferation ex vivo than treatment with DsCE-II or IL-2 alone. This DsCE-II fraction, which contains a polysaccharide with a high proportion of β-1,4-linkage mannose (≥64%), also promoted the regeneration of specific progenitor cell populations in damaged bone marrow tissues of 5-fluorouracil-treated mice. Colony-forming unit (CFU) analyses demonstrated that the population of CFU-GM cells, but not CFU-GEMM or BFU-E cells, preferentially recovered to ~67% in the bone marrow of immune-suppressed mice fed with DsCE-II. DsCE-II efficacy level was ~85% of that obtained by subcutaneous administration of recombinant G-CSF proteins (5 μg kg−1) in mice tested in parallel. This study suggests that the DsCE-II fraction of D. batatas extract may be considered for further development as a dietary supplement for use alongside chemotherapy during cancer treatment. |
| doi_str_mv | 10.1093/ecam/neq032 |
| format | Article |
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Consequently, alternative approaches are increasingly desirable. Dioscorea spp. tuber is a widely used traditional Chinese medicinal herb claimed to confer immunostimulatory activity. In this study, we evaluated Dioscorea as an adjuvant therapy for use alongside chemotherapy for cancer. Phytocompounds from Dioscorea tubers were ethanol fractioned and used for ex vivo splenocyte proliferation assay or in vivo force-feeding of mice pre-treated with the chemotherapy agent 5-fluorouracil. Co-treatment with a 50–75% ethanol-partitioned fraction of the tuber extract of D. batatas (DsCE-II) and interleukin (IL)-2 resulted in a significantly higher rate of murine splenocyte cell proliferation ex vivo than treatment with DsCE-II or IL-2 alone. This DsCE-II fraction, which contains a polysaccharide with a high proportion of β-1,4-linkage mannose (≥64%), also promoted the regeneration of specific progenitor cell populations in damaged bone marrow tissues of 5-fluorouracil-treated mice. Colony-forming unit (CFU) analyses demonstrated that the population of CFU-GM cells, but not CFU-GEMM or BFU-E cells, preferentially recovered to ~67% in the bone marrow of immune-suppressed mice fed with DsCE-II. DsCE-II efficacy level was ~85% of that obtained by subcutaneous administration of recombinant G-CSF proteins (5 μg kg−1) in mice tested in parallel. This study suggests that the DsCE-II fraction of D. batatas extract may be considered for further development as a dietary supplement for use alongside chemotherapy during cancer treatment.</description><identifier>ISSN: 1741-427X</identifier><identifier>EISSN: 1741-4288</identifier><identifier>DOI: 10.1093/ecam/neq032</identifier><identifier>PMID: 21799689</identifier><language>eng</language><publisher>Cairo, Egypt: Hindawi Puplishing Corporation</publisher><subject>5-Fluorouracil ; Animal tissues ; Bone growth ; Bone marrow ; Cancer ; Cell growth ; Cell proliferation ; Chemotherapy ; Cytotoxicity ; Dietary supplements ; Dioscorea ; Dioscorea alata ; Ethanol ; Force-feeding ; Granulocyte colony-stimulating factor ; Herbal medicine ; Immunology ; Immunostimulation ; Immunotherapy ; Interleukin 2 ; Laboratory animals ; Mannose ; Medical research ; Medicinal plants ; Medicine ; Mice ; Original ; Polysaccharides ; Progenitor cells ; Proteins ; Regeneration ; Science ; Stem cells ; Tubers</subject><ispartof>Evidence-based complementary and alternative medicine, 2011-01, Vol.2011 (2011), p.1-11</ispartof><rights>Copyright © 2011 Pei-Fen Su et al.</rights><rights>Copyright © 2011 Pei-Fen Su et al. This is an open access article distributed under the Creative Commons Attribution License (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. https://creativecommons.org/licenses/by/4.0</rights><rights>Copyright © 2011 Pei-Fen Su et al. 2011</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c428t-fed8454336d9c4425a53b7df2187de793e6020d34f0a9322f9adfe883991ae553</citedby><cites>FETCH-LOGICAL-c428t-fed8454336d9c4425a53b7df2187de793e6020d34f0a9322f9adfe883991ae553</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3137395/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3137395/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21799689$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Su, Pei-Fen</creatorcontrib><creatorcontrib>Li, Chin-Jin</creatorcontrib><creatorcontrib>Hsu, Chih-Chien</creatorcontrib><creatorcontrib>Benson, Spencer</creatorcontrib><creatorcontrib>Wang, Sheng-Yang</creatorcontrib><creatorcontrib>Aravindaram, Kandan</creatorcontrib><creatorcontrib>Chan, Sunney I.</creatorcontrib><creatorcontrib>Wu, Shih-Hsiung</creatorcontrib><creatorcontrib>Yang, Feng-Ling</creatorcontrib><creatorcontrib>Huang, Wen-Ching</creatorcontrib><creatorcontrib>Shyur, Lie-Fen</creatorcontrib><creatorcontrib>Yang, Ning-Sun</creatorcontrib><title>Dioscorea Phytocompounds Enhance Murine Splenocyte Proliferation Ex Vivo and Improve Regeneration of Bone Marrow Cells In Vivo</title><title>Evidence-based complementary and alternative medicine</title><addtitle>Evid Based Complement Alternat Med</addtitle><description>Specific cytokines have been tested clinically for immunotherapy of cancers; however, cytotoxicity has often impaired their usefulness. Consequently, alternative approaches are increasingly desirable. Dioscorea spp. tuber is a widely used traditional Chinese medicinal herb claimed to confer immunostimulatory activity. In this study, we evaluated Dioscorea as an adjuvant therapy for use alongside chemotherapy for cancer. Phytocompounds from Dioscorea tubers were ethanol fractioned and used for ex vivo splenocyte proliferation assay or in vivo force-feeding of mice pre-treated with the chemotherapy agent 5-fluorouracil. Co-treatment with a 50–75% ethanol-partitioned fraction of the tuber extract of D. batatas (DsCE-II) and interleukin (IL)-2 resulted in a significantly higher rate of murine splenocyte cell proliferation ex vivo than treatment with DsCE-II or IL-2 alone. This DsCE-II fraction, which contains a polysaccharide with a high proportion of β-1,4-linkage mannose (≥64%), also promoted the regeneration of specific progenitor cell populations in damaged bone marrow tissues of 5-fluorouracil-treated mice. Colony-forming unit (CFU) analyses demonstrated that the population of CFU-GM cells, but not CFU-GEMM or BFU-E cells, preferentially recovered to ~67% in the bone marrow of immune-suppressed mice fed with DsCE-II. DsCE-II efficacy level was ~85% of that obtained by subcutaneous administration of recombinant G-CSF proteins (5 μg kg−1) in mice tested in parallel. This study suggests that the DsCE-II fraction of D. batatas extract may be considered for further development as a dietary supplement for use alongside chemotherapy during cancer treatment.</description><subject>5-Fluorouracil</subject><subject>Animal tissues</subject><subject>Bone growth</subject><subject>Bone marrow</subject><subject>Cancer</subject><subject>Cell growth</subject><subject>Cell proliferation</subject><subject>Chemotherapy</subject><subject>Cytotoxicity</subject><subject>Dietary supplements</subject><subject>Dioscorea</subject><subject>Dioscorea alata</subject><subject>Ethanol</subject><subject>Force-feeding</subject><subject>Granulocyte colony-stimulating factor</subject><subject>Herbal medicine</subject><subject>Immunology</subject><subject>Immunostimulation</subject><subject>Immunotherapy</subject><subject>Interleukin 2</subject><subject>Laboratory animals</subject><subject>Mannose</subject><subject>Medical research</subject><subject>Medicinal plants</subject><subject>Medicine</subject><subject>Mice</subject><subject>Original</subject><subject>Polysaccharides</subject><subject>Progenitor cells</subject><subject>Proteins</subject><subject>Regeneration</subject><subject>Science</subject><subject>Stem 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Chih-Chien</au><au>Benson, Spencer</au><au>Wang, Sheng-Yang</au><au>Aravindaram, Kandan</au><au>Chan, Sunney I.</au><au>Wu, Shih-Hsiung</au><au>Yang, Feng-Ling</au><au>Huang, Wen-Ching</au><au>Shyur, Lie-Fen</au><au>Yang, Ning-Sun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Dioscorea Phytocompounds Enhance Murine Splenocyte Proliferation Ex Vivo and Improve Regeneration of Bone Marrow Cells In Vivo</atitle><jtitle>Evidence-based complementary and alternative medicine</jtitle><addtitle>Evid Based Complement Alternat Med</addtitle><date>2011-01-01</date><risdate>2011</risdate><volume>2011</volume><issue>2011</issue><spage>1</spage><epage>11</epage><pages>1-11</pages><issn>1741-427X</issn><eissn>1741-4288</eissn><abstract>Specific cytokines have been tested clinically for immunotherapy of cancers; however, cytotoxicity has often impaired their usefulness. Consequently, alternative approaches are increasingly desirable. Dioscorea spp. tuber is a widely used traditional Chinese medicinal herb claimed to confer immunostimulatory activity. In this study, we evaluated Dioscorea as an adjuvant therapy for use alongside chemotherapy for cancer. Phytocompounds from Dioscorea tubers were ethanol fractioned and used for ex vivo splenocyte proliferation assay or in vivo force-feeding of mice pre-treated with the chemotherapy agent 5-fluorouracil. Co-treatment with a 50–75% ethanol-partitioned fraction of the tuber extract of D. batatas (DsCE-II) and interleukin (IL)-2 resulted in a significantly higher rate of murine splenocyte cell proliferation ex vivo than treatment with DsCE-II or IL-2 alone. This DsCE-II fraction, which contains a polysaccharide with a high proportion of β-1,4-linkage mannose (≥64%), also promoted the regeneration of specific progenitor cell populations in damaged bone marrow tissues of 5-fluorouracil-treated mice. Colony-forming unit (CFU) analyses demonstrated that the population of CFU-GM cells, but not CFU-GEMM or BFU-E cells, preferentially recovered to ~67% in the bone marrow of immune-suppressed mice fed with DsCE-II. DsCE-II efficacy level was ~85% of that obtained by subcutaneous administration of recombinant G-CSF proteins (5 μg kg−1) in mice tested in parallel. This study suggests that the DsCE-II fraction of D. batatas extract may be considered for further development as a dietary supplement for use alongside chemotherapy during cancer treatment.</abstract><cop>Cairo, Egypt</cop><pub>Hindawi Puplishing Corporation</pub><pmid>21799689</pmid><doi>10.1093/ecam/neq032</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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| source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central Open Access; Wiley Online Library (Open Access Collection); PubMed Central; Alma/SFX Local Collection |
| subjects | 5-Fluorouracil Animal tissues Bone growth Bone marrow Cancer Cell growth Cell proliferation Chemotherapy Cytotoxicity Dietary supplements Dioscorea Dioscorea alata Ethanol Force-feeding Granulocyte colony-stimulating factor Herbal medicine Immunology Immunostimulation Immunotherapy Interleukin 2 Laboratory animals Mannose Medical research Medicinal plants Medicine Mice Original Polysaccharides Progenitor cells Proteins Regeneration Science Stem cells Tubers |
| title | Dioscorea Phytocompounds Enhance Murine Splenocyte Proliferation Ex Vivo and Improve Regeneration of Bone Marrow Cells In Vivo |
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