Native Electrospray and Electron-Capture Dissociation FTICR Mass Spectrometry for Top-Down Studies of Protein Assemblies

The high sensitivity, extended mass range, and fast data acquisition/processing of mass spectrometry and its coupling with native electrospray ionization (ESI) make the combination complementary to other biophysical methods of protein analysis. Protein assemblies with molecular masses up to MDa are...

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Veröffentlicht in:	Analytical chemistry (Washington) 2011-07, Vol.83 (14), p.5598-5606
Hauptverfasser:	Zhang, Hao, Cui, Weidong, Wen, Jianzhong, Blankenship, Robert E, Gross, Michael L
Format:	Artikel
Sprache:	eng
Schlagworte:	Alcohol Dehydrogenase - chemistry Amino Acid Sequence Amino acids Analytical chemistry Bacterial Proteins - chemistry BASIC BIOLOGICAL SCIENCES Binding sites Biological and medical sciences Canavalia - chemistry Chemistry Chlorobi - chemistry Concanavalin A - chemistry Crystallography Diverse techniques Electrons Equipment Design Exact sciences and technology Fourier Analysis Fundamental and applied biological sciences. Psychology Light-Harvesting Protein Complexes - chemistry Mass spectrometry Mass Spectrometry - instrumentation Mass Spectrometry - methods Models, Molecular Molecular and cellular biology Molecular Sequence Data Plant Proteins - chemistry Proteins Proteins - chemistry Saccharomyces cerevisiae - enzymology solar (fuels), photosynthesis (natural and artificial), biofuels (including algae and biomass), bio-inspired, charge transport, membrane, synthesis (novel materials), synthesis (self-assembly) Spectrometric and optical methods Spectrometry, Mass, Electrospray Ionization - instrumentation Spectrometry, Mass, Electrospray Ionization - methods
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